Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,507,749 G→T 92.5% H224N (CAT→AAT)  rpe ← D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,507,7490GT92.5% 812.1 / 60.1 280H224N (CAT→AAT) rpeD‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (8/13);  new base T (120/139);  total (128/152)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

Only 100 of 282 total aligned reads displayed.

CCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTC  >  CP009273/3507602‑3507896
                                                                                                                                                   |                                                                                                                                                   
 cAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACt                                                                                                                                                  <  2:21130/150‑1 (MQ=255)
   ggTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTa                                                                                                                                                <  2:4435742/150‑1 (MQ=255)
   ggTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTa                                                                                                                                                <  1:2316188/150‑1 (MQ=255)
   ggTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTa                                                                                                                                                <  2:3496709/150‑1 (MQ=255)
       ataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCtt                                                                                                                                            <  2:1041949/150‑1 (MQ=255)
            gcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCa                                                                                                                                       <  1:3283663/150‑1 (MQ=255)
               ttcttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGtt                                                                                                                                    >  2:4200022/1‑150 (MQ=255)
                 cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                  <  2:3471885/150‑1 (MQ=255)
                 cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                  >  2:1531403/1‑150 (MQ=255)
                 cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                  <  2:1874253/150‑1 (MQ=255)
                  ttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCTGCGTACAATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCAc                                                                                                                                 <  2:1733865/150‑1 (MQ=255)
                   tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                                <  2:1280672/150‑1 (MQ=255)
                   tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                                <  2:426781/150‑1 (MQ=255)
                     aCCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTgc                                                                                                                              <  1:4516772/150‑1 (MQ=255)
                       cTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACAGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTgcgc                                                                                                                            >  1:2723275/1‑150 (MQ=255)
                          cGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAtt                                                                                                                         <  1:2208929/150‑1 (MQ=255)
                           gACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                        >  1:166789/1‑150 (MQ=255)
                               ggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcatc                                                                                                                    <  1:1236415/150‑1 (MQ=255)
                               ggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcatc                                                                                                                    <  1:690912/150‑1 (MQ=255)
                                gCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcatca                                                                                                                   <  1:2922928/150‑1 (MQ=255)
                                 cAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCACGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTAGCCAGTTCACTGCGCATTTCATCaa                                                                                                                  >  2:2539532/1‑150 (MQ=255)
                                  aaCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAAt                                                                                                                 >  1:4562981/1‑150 (MQ=255)
                                  aaCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAAt                                                                                                                 >  1:2135246/1‑150 (MQ=255)
                                  aaCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAAt                                                                                                                 >  2:550985/1‑150 (MQ=255)
                                   aCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATg                                                                                                                <  2:3987189/150‑1 (MQ=255)
                                   aCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATg                                                                                                                <  2:4388287/150‑1 (MQ=255)
                                    cTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGa                                                                                                               >  1:2251285/1‑150 (MQ=255)
                                        aGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACttt                                                                                                           <  2:2557792/150‑1 (MQ=255)
                                             aTACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGt                                                                                                      >  2:867/1‑150 (MQ=255)
                                                cAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGt                                                                                                   <  2:2451196/150‑1 (MQ=255)
                                                    gcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctgg                                                                                               >  1:1977755/1‑150 (MQ=255)
                                                      cATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggct                                                                                             >  2:3543672/1‑150 (MQ=255)
                                                      cATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggct                                                                                             <  2:665716/150‑1 (MQ=255)
                                                       atatCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctg                                                                                            >  1:1405341/1‑150 (MQ=255)
                                                           cTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCg                                                                                        <  1:2354075/150‑1 (MQ=255)
                                                           cTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCg                                                                                        <  1:2821337/150‑1 (MQ=255)
                                                           cTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCg                                                                                        <  1:4249221/150‑1 (MQ=255)
                                                            tACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGa                                                                                       >  2:3029231/1‑150 (MQ=255)
                                                            tACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGa                                                                                       <  2:111354/150‑1 (MQ=255)
                                                             aCCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGaa                                                                                      >  1:402842/1‑150 (MQ=255)
                                                             aCCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGaa                                                                                      <  1:2623406/150‑1 (MQ=255)
                                                             aCCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGaa                                                                                      >  1:3562986/1‑150 (MQ=255)
                                                              ccGCAGCAGCAAGACCAGGAGCACTTTCGACAAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATAAATGACTTTTTTGTAGTCTGGCTGGTCGAAg                                                                                     >  2:811624/1‑150 (MQ=255)
                                                                gcagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAt                                                                                   <  1:3201402/150‑1 (MQ=255)
                                                                gcagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAt                                                                                   <  1:1009013/150‑1 (MQ=255)
                                                                 cagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAtt                                                                                  >  2:2914440/1‑150 (MQ=255)
                                                                  agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTg                                                                                 >  2:2698914/1‑150 (MQ=255)
                                                                  agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTg                                                                                 >  1:156172/1‑150 (MQ=255)
                                                                   gcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGc                                                                                >  2:1690874/1‑150 (MQ=255)
                                                                        aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAc                                                                           >  1:3649738/1‑150 (MQ=255)
                                                                        aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAc                                                                           >  1:3649736/1‑150 (MQ=255)
                                                                         aGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTTTAGTCTGGCTGGTCGAAGATTGCCGAAcc                                                                          <  2:3431726/150‑1 (MQ=255)
                                                                            ccAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGc                                                                       <  2:4377824/150‑1 (MQ=255)
                                                                            ccAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGc                                                                       <  2:715876/150‑1 (MQ=255)
                                                                             cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcg                                                                      <  2:3171721/150‑1 (MQ=255)
                                                                             cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcg                                                                      <  1:2469059/150‑1 (MQ=255)
                                                                              aGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAAAGACTTTTTTTTAGTCTGTCTGGTCGAAGATTGCCGAACCGGcga                                                                     >  1:1976117/1‑150 (MQ=255)
                                                                                   cACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAAc                                                                <  2:2427282/150‑1 (MQ=255)
                                                                                      tGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACata                                                             <  2:157958/150‑1 (MQ=255)
                                                                                             cAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCgcgc                                                      >  1:81505/1‑150 (MQ=255)
                                                                                              aGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                     >  2:4088791/1‑150 (MQ=255)
                                                                                              aGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                     <  1:3142923/150‑1 (MQ=255)
                                                                                                cGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccg                                                   >  1:654708/1‑150 (MQ=255)
                                                                                                  tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgcc                                                 <  2:2723010/150‑1 (MQ=255)
                                                                                                  tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgcc                                                 <  2:2723342/150‑1 (MQ=255)
                                                                                                  tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgcc                                                 <  2:3287439/150‑1 (MQ=255)
                                                                                                       tCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGc                                            >  2:2329723/1‑150 (MQ=255)
                                                                                                        cAAGATCAAAAGCGACGCAGCGAAAAACTTCAAACTAAATCATTACTTACCTTTGCCAGTTCACTGCGCCAATCATCAATGACTTTTTTGTAGTCAGGCTGGTCGAAGATTGCCGAACCGGCGACGAACAAATCCGCGCCCGCCGCAGCg                                           >  2:734955/1‑150 (MQ=255)
                                                                                                         aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGa                                          >  1:3694093/1‑150 (MQ=255)
                                                                                                         aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGa                                          >  2:1307910/1‑150 (MQ=255)
                                                                                                                aaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGcc                                   >  1:273239/1‑150 (MQ=255)
                                                                                                                   gCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAt                                <  1:1027339/150‑1 (MQ=255)
                                                                                                                    cGACGCCGCGACTATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTGTAGTAGTCAGGCTGGTCGAAGATTGCCGAACCGGCCACGAACATATACGCGCCCGCCGCAGCGATTACGCCACtg                               >  1:3489552/1‑150 (MQ=255)
                                                                                                                    cGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtg                               >  2:3399224/1‑150 (MQ=255)
                                                                                                                     gACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgt                              >  1:764532/1‑150 (MQ=255)
                                                                                                                     gACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgt                              >  2:4028672/1‑150 (MQ=255)
                                                                                                                     gACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgt                              >  2:3781628/1‑150 (MQ=255)
                                                                                                                       cgccgcGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgttg                            <  2:3109986/150‑1 (MQ=255)
                                                                                                                        gccgcGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgttgt                           <  2:138161/150‑1 (MQ=255)
                                                                                                                         ccgcGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATgttgtt                          >  1:3252271/1‑150 (MQ=255)
                                                                                                                          cgcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                         <  1:3121111/150‑1 (MQ=255)
                                                                                                                          cgcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                         <  1:2495580/150‑1 (MQ=255)
                                                                                                                            cgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAc                       >  2:4562437/1‑150 (MQ=255)
                                                                                                                              aaTATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCt                     <  2:3508131/150‑1 (MQ=255)
                                                                                                                              aaTATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCt                     <  2:4338692/150‑1 (MQ=255)
                                                                                                                              aaTATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCt                     <  1:4228474/150‑1 (MQ=255)
                                                                                                                                 atCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCa                  >  2:1950718/1‑150 (MQ=255)
                                                                                                                                  tCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCAc                 >  1:3607426/1‑150 (MQ=255)
                                                                                                                                   cTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACg                <  1:475750/150‑1 (MQ=255)
                                                                                                                                    ttCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGc               >  2:2930598/1‑150 (MQ=255)
                                                                                                                                     tCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGcc              >  1:1306684/1‑150 (MQ=255)
                                                                                                                                       aaaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAc            >  1:1577843/1‑150 (MQ=255)
                                                                                                                                        aaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAcc           <  1:3364218/150‑1 (MQ=255)
                                                                                                                                        aaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAcc           >  1:4100141/1‑150 (MQ=255)
                                                                                                                                         aCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCg          >  2:1457279/1‑150 (MQ=255)
                                                                                                                                           ttATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTc        <  2:3310609/150‑1 (MQ=255)
                                                                                                                                            tATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTcc       >  1:2446391/1‑150 (MQ=255)
                                                                                                                                               tCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACt    <  2:602028/150‑1 (MQ=255)
                                                                                                                                                cATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACtt   >  1:691854/1‑150 (MQ=255)
                                                                                                                                                 aTGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTc  >  1:2905956/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTC  >  CP009273/3507602‑3507896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑