Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,525,950 C→T 5.7% intergenic (‑152/‑227) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,525,9500CT 5.7% 1077.8 / 32.3 297intergenic (‑152/‑227)yhgE/pckDUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  ref base C (150/130);  new base T (10/7);  total (160/137)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 297 total aligned reads displayed.

GGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAA  >  CP009273/3525802‑3526097
                                                                                                                                                    |                                                                                                                                                   
ggTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt                                                                                                                                                    >  1:914449/1‑150 (MQ=255)
ggTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt                                                                                                                                                    >  1:788695/1‑150 (MQ=255)
ggTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt                                                                                                                                                    <  1:2606240/150‑1 (MQ=255)
 gTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTTGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATTAATTTCATTCAGGAATGCGATTCCACTc                                                                                                                                                   <  2:3867709/150‑1 (MQ=255)
   tATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTcac                                                                                                                                                 <  1:2047284/150‑1 (MQ=255)
            tCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTccc                                                                                                                                        >  1:185869/1‑150 (MQ=255)
            tCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTccc                                                                                                                                        <  2:3957378/150‑1 (MQ=255)
             cAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCg                                                                                                                                       >  2:231629/1‑150 (MQ=255)
             cAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCg                                                                                                                                       >  1:4072600/1‑150 (MQ=255)
              aaaaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGc                                                                                                                                      <  1:670529/150‑1 (MQ=255)
              aaaaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGc                                                                                                                                      >  1:2324549/1‑150 (MQ=255)
               aaaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGTATATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCAATTCCATTCACAATATTCCCGcc                                                                                                                                     <  1:2455716/150‑1 (MQ=255)
               aaaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGcc                                                                                                                                     >  1:3096232/1‑150 (MQ=255)
                  ggTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCata                                                                                                                                  >  1:1129386/1‑150 (MQ=255)
                  ggTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCata                                                                                                                                  >  2:2387475/1‑150 (MQ=255)
                  ggTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCata                                                                                                                                  <  1:741999/150‑1 (MQ=255)
                    tGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCAtata                                                                                                                                >  2:56218/1‑150 (MQ=255)
                     gggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATaa                                                                                                                               <  1:1735772/150‑1 (MQ=255)
                         tAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCa                                                                                                                           >  1:1586846/1‑150 (MQ=255)
                          aaTTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGACTCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATTCGATTCCACTCACAATATTCCCGCCATATAAACCaa                                                                                                                          >  1:4183415/1‑150 (MQ=255)
                           aTTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAg                                                                                                                         >  1:4299423/1‑150 (MQ=255)
                             tATCGCATCCGGGCAGTAGTATTTTGATTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATAATTTACCGGTTTAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAatat                                                                                                                       <  1:819518/150‑1 (MQ=255)
                              aTCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAtt                                                                                                                      <  2:226544/150‑1 (MQ=255)
                              aTCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAtt                                                                                                                      <  2:2542560/150‑1 (MQ=255)
                              aTCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTATACCGGTTGAATTTGCAACAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAtt                                                                                                                      >  2:4244935/1‑150 (MQ=255)
                               tCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGAttt                                                                                                                     <  2:4585354/150‑1 (MQ=255)
                               tCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAttt                                                                                                                     <  2:4443835/150‑1 (MQ=255)
                                 gCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTaa                                                                                                                   >  2:1458039/1‑150 (MQ=255)
                                  cATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAAc                                                                                                                  <  2:4332181/150‑1 (MQ=255)
                                   aTCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAAcc                                                                                                                 >  1:4310112/1‑150 (MQ=255)
                                     ccGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCtt                                                                                                               <  2:181115/150‑1 (MQ=255)
                                         gaagtagtAATTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAAAAGATACTTTACCGTTTGAATTTTCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCAAATAAACCAAGATTAAACCttttta                                                                                                           >  1:3171109/3‑148 (MQ=255)
                                         gCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTga                                                                                                           >  1:3171159/1‑150 (MQ=255)
                                               gtaTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAt                                                                                                     >  2:3095089/1‑150 (MQ=255)
                                                taTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAtt                                                                                                    >  2:2801908/1‑150 (MQ=255)
                                                 aTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTAAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAttt                                                                                                   <  1:2927536/150‑1 (MQ=255)
                                                   tttGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTc                                                                                                 >  1:3937489/1‑150 (MQ=255)
                                                   tttGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTc                                                                                                 >  2:873598/1‑150 (MQ=255)
                                                     tGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCca                                                                                               <  2:639704/150‑1 (MQ=255)
                                                       cTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCcaca                                                                                             >  1:3801462/1‑150 (MQ=255)
                                                        ttttttCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCacac                                                                                            <  2:4135087/150‑1 (MQ=255)
                                                           tttCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTa                                                                                         <  1:3708029/150‑1 (MQ=255)
                                                                      aatCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAttt                                                                              >  2:1577294/1‑150 (MQ=255)
                                                                      aatCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAttt                                                                              >  2:3302605/1‑150 (MQ=255)
                                                                       atCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTc                                                                             <  1:273222/150‑1 (MQ=255)
                                                                        tCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCt                                                                            >  1:1448083/1‑150 (MQ=255)
                                                                         cAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGTTTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTg                                                                           <  2:2086010/150‑1 (MQ=255)
                                                                         cAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTg                                                                           <  2:4416108/150‑1 (MQ=255)
                                                                         cAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTg                                                                           <  1:2494476/150‑1 (MQ=255)
                                                                         cAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTg                                                                           >  1:3625043/1‑150 (MQ=255)
                                                                         cAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTg                                                                           >  1:3164/1‑150 (MQ=255)
                                                                          aaaaaaaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGc                                                                          >  1:3592645/1‑150 (MQ=255)
                                                                            aaaaaGTTAGCGTGGTGACTCGATACTATAACGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATAAAAACCAAGATTAAACCTTTTGAGAACATTATCCACAACTAAAATCCTATATATGCGa                                                                        >  1:4625628/1‑150 (MQ=255)
                                                                               aaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataa                                                                     >  2:471836/1‑150 (MQ=255)
                                                                                aGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataat                                                                    >  1:3314937/1‑150 (MQ=255)
                                                                                aGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataat                                                                    >  2:244508/1‑150 (MQ=255)
                                                                                aGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataat                                                                    >  2:1740167/1‑150 (MQ=255)
                                                                                 gTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                   <  2:3839316/150‑1 (MQ=255)
                                                                                 gTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                   >  1:4360854/1‑150 (MQ=255)
                                                                                  ttAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAg                                                                  <  2:3657341/150‑1 (MQ=255)
                                                                                      cGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAAc                                                              >  2:3523459/1‑150 (MQ=255)
                                                                                      cGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAAc                                                              >  1:2169865/1‑150 (MQ=255)
                                                                                       gtggtgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAAcc                                                             >  1:361390/1‑150 (MQ=255)
                                                                                          gtgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGtt                                                          >  1:3903678/1‑150 (MQ=255)
                                                                                           tgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGttt                                                         <  1:4632015/150‑1 (MQ=255)
                                                                                           tgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGttt                                                         <  1:1323938/150‑1 (MQ=255)
                                                                                           tgAATCGATACTTTACAGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGttt                                                         <  2:2677136/150‑1 (MQ=255)
                                                                                            gAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTc                                                        <  2:3897461/150‑1 (MQ=255)
                                                                                                cGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGa                                                    >  1:101597/1‑150 (MQ=255)
                                                                                                    aCTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGTTTTAACCTTTTGAGAACATTTTCCACACCTAACATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAgg                                                <  2:850904/150‑1 (MQ=255)
                                                                                                      tttACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGaa                                              <  1:4397401/150‑1 (MQ=255)
                                                                                                      tttACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGaa                                              <  2:1102786/150‑1 (MQ=255)
                                                                                                           cGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACg                                         >  1:295307/1‑150 (MQ=255)
                                                                                                            ggTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACgg                                        <  2:1965428/150‑1 (MQ=255)
                                                                                                            ggTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACgg                                        >  2:148516/1‑150 (MQ=255)
                                                                                                             gTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGa                                       >  2:771451/1‑150 (MQ=255)
                                                                                                              ttGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATCTTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAg                                      <  2:3650024/150‑1 (MQ=255)
                                                                                                                 aaTTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGttt                                   >  2:1327119/1‑150 (MQ=255)
                                                                                                                  aTTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGtttt                                  <  1:1742737/150‑1 (MQ=255)
                                                                                                                   tttGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGttttt                                 <  1:4136943/150‑1 (MQ=255)
                                                                                                                   tttGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGttttt                                 <  2:1266711/150‑1 (MQ=255)
                                                                                                                      gCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTGTTGAGAACATTTTCCACACCTAAAATGCAATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGt                              >  1:3521609/1‑150 (MQ=255)
                                                                                                                       cATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTc                             >  2:4202358/1‑150 (MQ=255)
                                                                                                                          cAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCaaa                          <  2:914449/150‑1 (MQ=255)
                                                                                                                             tttCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAatat                       <  2:1046816/150‑1 (MQ=255)
                                                                                                                               tcattcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGa                     <  2:4301647/150‑1 (MQ=255)
                                                                                                                               tcattcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGa                     <  2:1624198/150‑1 (MQ=255)
                                                                                                                                cattcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGaa                    <  2:2480936/150‑1 (MQ=255)
                                                                                                                                  ttcaGGAATGCGACTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATTAAtt                  >  2:3754997/1‑150 (MQ=255)
                                                                                                                                    caGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtc                >  2:4177677/1‑150 (MQ=255)
                                                                                                                                    caGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtc                <  2:3592674/150‑1 (MQ=255)
                                                                                                                                    caGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtc                >  2:1847093/1‑150 (MQ=255)
                                                                                                                                     aGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtct               <  2:2000640/150‑1 (MQ=255)
                                                                                                                                      ggAATGCGATTCCACTCACAATATTCCAGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtctc              <  2:3407028/150‑1 (MQ=255)
                                                                                                                                       gAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTcc             <  1:500410/150‑1 (MQ=255)
                                                                                                                                         aTGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAg           >  2:1159214/1‑150 (MQ=255)
                                                                                                                                           gCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGAt         <  2:3093345/150‑1 (MQ=255)
                                                                                                                                            cGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATa        >  1:3455761/1‑150 (MQ=255)
                                                                                                                                                 ccACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTaa   <  1:2879951/150‑1 (MQ=255)
                                                                                                                                                  cACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTaaa  >  2:1176800/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAA  >  CP009273/3525802‑3526097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑