Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,568,708 C→A 18.2% S93* (TCA→TAA)  yhgN → UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,568,7080CA18.2% 920.8 / 124.7 297S93* (TCA→TAA) yhgNUPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (137/105);  new base A (36/19);  total (173/124)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTCTGC  >  CP009273/3568560‑3568857
                                                                                                                                                    |                                                                                                                                                     
ttGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCa                                                                                                                                                      >  1:87662/1‑150 (MQ=255)
  gCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAgg                                                                                                                                                    <  1:2414740/150‑1 (MQ=255)
   cTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGa                                                                                                                                                   >  1:547877/1‑150 (MQ=255)
   cTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGa                                                                                                                                                   >  2:2231955/1‑150 (MQ=255)
     tATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGaaa                                                                                                                                                 >  2:2750716/1‑150 (MQ=255)
      aTTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAAt                                                                                                                                                >  2:4004918/1‑150 (MQ=255)
       ttGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATa                                                                                                                                               >  1:3400519/1‑150 (MQ=255)
          ctctCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGATAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATagca                                                                                                                                            <  1:3915625/150‑1 (MQ=255)
          ctctCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATagca                                                                                                                                            <  2:1478137/150‑1 (MQ=255)
             tcCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCg                                                                                                                                         <  1:970990/150‑1 (MQ=255)
                tGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGc                                                                                                                                      <  2:433134/150‑1 (MQ=255)
                tGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGc                                                                                                                                      >  2:850646/1‑150 (MQ=255)
                 ggTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCt                                                                                                                                     <  1:1198701/150‑1 (MQ=255)
                    gATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTcc                                                                                                                                  <  1:1869101/150‑1 (MQ=255)
                    gATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTcc                                                                                                                                  >  1:3824064/1‑150 (MQ=255)
                    gATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTcc                                                                                                                                  <  2:486942/150‑1 (MQ=255)
                     aTGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCg                                                                                                                                 >  2:4060590/1‑150 (MQ=255)
                     aTGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCg                                                                                                                                 >  1:4037545/1‑150 (MQ=255)
                      tGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCgg                                                                                                                                <  1:974614/150‑1 (MQ=255)
                        cTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCa                                                                                                                              >  1:2055087/1‑150 (MQ=255)
                          ggTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAgg                                                                                                                            <  1:1930023/150‑1 (MQ=255)
                           gtgtTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGt                                                                                                                           <  2:1431672/150‑1 (MQ=255)
                            tgtTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTg                                                                                                                          <  2:1827993/150‑1 (MQ=255)
                            tgtTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTg                                                                                                                          <  2:3809524/150‑1 (MQ=255)
                            tgtTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTg                                                                                                                          <  1:1376343/150‑1 (MQ=255)
                              ttCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGaa                                                                                                                        >  1:2808549/1‑150 (MQ=255)
                               tCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAag                                                                                                                       <  2:3435270/150‑1 (MQ=255)
                                 cTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAagag                                                                                                                     >  1:952177/1‑150 (MQ=255)
                                       gCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCAttt                                                                                                               <  1:3287478/150‑1 (MQ=255)
                                       gCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCAttt                                                                                                               <  1:3319003/150‑1 (MQ=255)
                                        cGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTa                                                                                                              >  2:4533554/1‑150 (MQ=255)
                                         gggTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTAt                                                                                                             >  2:2271364/1‑150 (MQ=255)
                                            tGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGt                                                                                                          >  1:609854/1‑150 (MQ=255)
                                             gagaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTg                                                                                                         >  1:3074874/1‑150 (MQ=255)
                                             gagaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTg                                                                                                         >  1:1637126/1‑150 (MQ=255)
                                              agaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGc                                                                                                        >  2:198485/1‑150 (MQ=255)
                                               gaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGcc                                                                                                       >  1:1776732/1‑150 (MQ=255)
                                               gaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGcc                                                                                                       <  1:1419798/150‑1 (MQ=255)
                                               gaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGcc                                                                                                       >  1:726521/1‑150 (MQ=255)
                                                 aaaTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGt                                                                                                     <  2:2062982/150‑1 (MQ=255)
                                                  aaTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGtt                                                                                                    <  2:2027828/150‑1 (MQ=255)
                                                   aTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTg                                                                                                   >  2:424533/1‑150 (MQ=255)
                                                   aTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTg                                                                                                   >  2:4569381/1‑150 (MQ=255)
                                                    ttCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTgg                                                                                                  <  2:322036/150‑1 (MQ=255)
                                                        ggCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCTTCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAAt                                                                                              <  1:3969400/150‑1 (MQ=255)
                                                          cATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTc                                                                                            >  1:803605/1‑150 (MQ=255)
                                                           aTTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTcc                                                                                           >  1:2112517/1‑150 (MQ=255)
                                                               cTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTa                                                                                       >  1:721211/1‑150 (MQ=255)
                                                                ttAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAg                                                                                      <  1:3032575/150‑1 (MQ=255)
                                                                 tAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGt                                                                                     >  1:1007705/1‑150 (MQ=255)
                                                                  aGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTc                                                                                    >  2:3513751/1‑150 (MQ=255)
                                                                  aGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTc                                                                                    <  2:4358179/150‑1 (MQ=255)
                                                                         gAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGc                                                                             <  1:4236026/150‑1 (MQ=255)
                                                                          aGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGcc                                                                            >  2:3235080/1‑150 (MQ=255)
                                                                            cAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGa                                                                          >  1:2585049/1‑150 (MQ=255)
                                                                            cAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGa                                                                          >  1:3624444/1‑150 (MQ=255)
                                                                              gAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACt                                                                        >  2:4486788/1‑150 (MQ=255)
                                                                               aaaCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATAAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGATAGTCGCCGGGCCGACTa                                                                       >  1:2513289/1‑150 (MQ=255)
                                                                                aaCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTAt                                                                      <  1:1338050/150‑1 (MQ=255)
                                                                                aaCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTAt                                                                      >  1:3624917/1‑150 (MQ=255)
                                                                                 aCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAAGAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTAtt                                                                     <  2:651235/150‑1 (MQ=255)
                                                                                  ccGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtc                                                                    >  2:2941715/1‑150 (MQ=255)
                                                                                  ccGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtc                                                                    >  1:2290088/1‑150 (MQ=255)
                                                                                   cGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtct                                                                   >  2:91663/1‑150 (MQ=255)
                                                                                   cGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtct                                                                   <  1:1510253/150‑1 (MQ=255)
                                                                                   cGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtct                                                                   >  1:2656493/1‑150 (MQ=255)
                                                                                    gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                  >  2:2384602/1‑150 (MQ=255)
                                                                                       tcCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGcc                                                               >  2:1033825/1‑150 (MQ=255)
                                                                                          aTTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACg                                                            >  1:3052530/1‑150 (MQ=255)
                                                                                                ggcggcATCATTCTGTTTCTGATCGCCATTAAAATGATTGTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtg                                                      <  2:1373615/150‑1 (MQ=255)
                                                                                                  cggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTTCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgtt                                                    <  2:3058549/150‑1 (MQ=255)
                                                                                                  cggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgtt                                                    >  2:1768786/1‑150 (MQ=255)
                                                                                                    gcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgt                                                  <  1:2532495/150‑1 (MQ=255)
                                                                                                           tCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtca                                           <  1:1067523/150‑1 (MQ=255)
                                                                                                            cTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcat                                          <  1:474037/150‑1 (MQ=255)
                                                                                                             tGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcatc                                         >  2:2396458/1‑150 (MQ=255)
                                                                                                              gTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcatca                                        <  2:3571301/150‑1 (MQ=255)
                                                                                                              gTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcatca                                        <  2:547877/150‑1 (MQ=255)
                                                                                                              gTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcatca                                        >  2:1104657/1‑150 (MQ=255)
                                                                                                                        gCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAAt                              <  1:2879056/150‑1 (MQ=255)
                                                                                                                         ccATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATc                             >  1:2184081/1‑150 (MQ=255)
                                                                                                                             tAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGAt                         <  2:2865058/150‑1 (MQ=255)
                                                                                                                             tAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGAt                         >  1:3109819/1‑150 (MQ=255)
                                                                                                                              aaaaTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATg                        <  1:2004924/150‑1 (MQ=255)
                                                                                                                                 aTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATgggg                     <  2:3161075/150‑1 (MQ=255)
                                                                                                                                 aTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATgggg                     >  1:2998965/1‑150 (MQ=255)
                                                                                                                                  tGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGc                    >  1:2316450/1‑150 (MQ=255)
                                                                                                                                    aTTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCAt                  >  2:428821/1‑150 (MQ=255)
                                                                                                                                    aTTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTAGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCAt                  >  2:698972/1‑150 (MQ=255)
                                                                                                                                     ttttCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATc                 <  2:1707037/150‑1 (MQ=255)
                                                                                                                                      tttCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCt                <  2:1539579/150‑1 (MQ=255)
                                                                                                                                       ttCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTg               <  1:4533554/150‑1 (MQ=255)
                                                                                                                                        tCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTgg              >  2:313134/1‑150 (MQ=255)
                                                                                                                                        tCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTgg              <  1:4314606/150‑1 (MQ=255)
                                                                                                                                             aGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGAtt         >  2:3321115/1‑150 (MQ=255)
                                                                                                                                             aGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGAtt         >  2:2069288/1‑150 (MQ=255)
                                                                                                                                                gcTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGct      >  1:3422035/1‑150 (MQ=255)
                                                                                                                                                gcTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGct      >  1:3686364/1‑150 (MQ=255)
                                                                                                                                                  ttCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGctct    <  2:1169390/150‑1 (MQ=255)
                                                                                                                                                    cAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTctgc  <  2:4197268/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTCTGC  >  CP009273/3568560‑3568857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑