Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,679,324 C→A 100% M1070I (ATG→ATT)  bcsC ← cellulose synthase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,679,3240CA97.8% 919.5 / 8.7 271M1070I (ATG→ATT) bcsCcellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (151/114);  total (156/115)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

CTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCC  >  CP009273/3679177‑3679470
                                                                                                                                                   |                                                                                                                                                  
ctgGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaat                                                                                                                                                  >  1:316270/1‑150 (MQ=255)
ctgGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaat                                                                                                                                                  >  1:3102906/1‑150 (MQ=255)
  ggATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaatca                                                                                                                                                <  1:2887850/150‑1 (MQ=255)
     tatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCg                                                                                                                                             >  2:2990417/1‑150 (MQ=255)
      atCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGACCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGACGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCgg                                                                                                                                            >  1:1426787/1‑150 (MQ=255)
       tCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGa                                                                                                                                           >  1:1979987/1‑150 (MQ=255)
       tCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGa                                                                                                                                           >  2:41195/1‑150 (MQ=255)
        cAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGAt                                                                                                                                          >  1:4206260/1‑150 (MQ=255)
         aaTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATa                                                                                                                                         >  1:2303258/1‑150 (MQ=255)
          aTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATaa                                                                                                                                        <  1:151574/150‑1 (MQ=255)
           ttGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAAc                                                                                                                                       <  2:1668403/150‑1 (MQ=255)
              ccGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAAcgcg                                                                                                                                    >  2:4269226/1‑150 (MQ=255)
              ccGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAAcgcg                                                                                                                                    >  1:4340633/1‑150 (MQ=255)
               cGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCgg                                                                                                                                   <  1:3279299/150‑1 (MQ=255)
               cGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCgg                                                                                                                                   <  1:3827090/150‑1 (MQ=255)
               cGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCgg                                                                                                                                   <  2:2279462/150‑1 (MQ=255)
                gTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGc                                                                                                                                  <  1:2581121/150‑1 (MQ=255)
                gTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGc                                                                                                                                  >  2:182145/1‑150 (MQ=255)
                    caacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATgg                                                                                                                              >  1:2959319/1‑150 (MQ=255)
                     aacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGt                                                                                                                             >  1:419081/1‑150 (MQ=255)
                       caaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGttt                                                                                                                           >  1:805453/1‑150 (MQ=255)
                       caaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGttt                                                                                                                           >  1:4546783/1‑150 (MQ=255)
                        aaaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                          <  2:3470266/150‑1 (MQ=255)
                        aaaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                          <  2:1382830/150‑1 (MQ=255)
                        aaaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                          >  2:4186985/1‑150 (MQ=255)
                        aaaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                          <  1:1238542/150‑1 (MQ=255)
                        aaaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                          <  2:519306/150‑1 (MQ=255)
                         aaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTg                                                                                                                         >  2:3716503/1‑150 (MQ=255)
                         aaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTg                                                                                                                         >  1:1170312/1‑150 (MQ=255)
                            cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      <  2:1006138/150‑1 (MQ=255)
                            cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      >  2:242289/1‑150 (MQ=255)
                            cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      <  1:4329477/150‑1 (MQ=255)
                            cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      <  1:1602327/150‑1 (MQ=255)
                            cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      >  2:2409450/1‑150 (MQ=255)
                                  gAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGaa                                                                                                                >  2:3539443/1‑150 (MQ=255)
                                  gAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGaa                                                                                                                >  2:2157034/1‑150 (MQ=255)
                                     gTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGc                                                                                                             >  1:4063601/1‑150 (MQ=255)
                                     gTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGc                                                                                                             <  2:2570802/150‑1 (MQ=255)
                                      tAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                            >  2:1547544/1‑150 (MQ=255)
                                      tAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                            <  1:2808692/150‑1 (MQ=255)
                                         cacGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGAcc                                                                                                         <  2:835236/150‑1 (MQ=255)
                                          acGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCa                                                                                                        <  2:3722827/150‑1 (MQ=255)
                                           cgtcgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCAc                                                                                                       >  2:2630752/1‑150 (MQ=255)
                                           cgtcgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCAc                                                                                                       <  1:2098598/150‑1 (MQ=255)
                                              cgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAg                                                                                                    >  2:1674814/1‑150 (MQ=255)
                                              cgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAg                                                                                                    <  1:2745678/150‑1 (MQ=255)
                                               gtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGc                                                                                                   >  2:4586065/1‑150 (MQ=255)
                                                 tCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCa                                                                                                 <  2:233844/150‑1 (MQ=255)
                                                  cAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAg                                                                                                >  2:3926848/1‑150 (MQ=255)
                                                  cAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAg                                                                                                <  1:2533252/150‑1 (MQ=255)
                                                    aGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGAc                                                                                              <  2:1949556/150‑1 (MQ=255)
                                                      tAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGc                                                                                            >  1:1797020/1‑150 (MQ=255)
                                                      tAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGc                                                                                            >  1:1796988/1‑150 (MQ=255)
                                                          gCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAccc                                                                                        >  2:3089411/1‑150 (MQ=255)
                                                           cccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCa                                                                                       >  2:1041522/1‑150 (MQ=255)
                                                           cccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCa                                                                                       >  2:1419392/1‑150 (MQ=255)
                                                           cccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCa                                                                                       >  2:3743541/1‑150 (MQ=255)
                                                            ccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAg                                                                                      <  1:4169851/150‑1 (MQ=255)
                                                                  cGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCa                                                                                >  1:2828230/1‑150 (MQ=255)
                                                                   gtgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                               >  2:3959991/1‑150 (MQ=255)
                                                                   gtgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                               >  2:2370246/1‑150 (MQ=255)
                                                                       aGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGAcc                                                                           <  1:4334337/150‑1 (MQ=255)
                                                                           gAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                       <  2:6390/150‑1 (MQ=255)
                                                                              gCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTc                                                                    >  2:3293121/1‑150 (MQ=255)
                                                                               cccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTcc                                                                   >  1:2386077/1‑150 (MQ=255)
                                                                               cccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTcc                                                                   >  1:73604/1‑150 (MQ=255)
                                                                                  tGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTg                                                                >  1:3541953/1‑150 (MQ=255)
                                                                                  tGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTg                                                                >  1:3632649/1‑150 (MQ=255)
                                                                                     ctgctgCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgcgc                                                             <  2:852839/150‑1 (MQ=255)
                                                                                      tgctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCt                                                            <  1:2342951/150‑1 (MQ=255)
                                                                                         tgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTccc                                                         >  1:341243/1‑150 (MQ=255)
                                                                                           ccgccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGc                                                       >  1:1370618/1‑150 (MQ=255)
                                                                                            cgccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGcc                                                      >  2:3680637/1‑150 (MQ=255)
                                                                                              ccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCcac                                                    >  1:1764963/1‑150 (MQ=255)
                                                                                               cATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCcaca                                                   <  1:1011560/150‑1 (MQ=255)
                                                                                               cATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCcaca                                                   >  2:336993/1‑150 (MQ=255)
                                                                                                   gTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAc                                               <  1:460204/150‑1 (MQ=255)
                                                                                                     tGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCg                                             >  2:2051197/1‑150 (MQ=255)
                                                                                                         ttGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTAt                                         <  2:2107031/150‑1 (MQ=255)
                                                                                                          tGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATg                                        >  2:4469373/1‑150 (MQ=255)
                                                                                                             cgcgCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCa                                     >  1:3070383/1‑150 (MQ=255)
                                                                                                                gcAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAAc                                  <  2:3248568/150‑1 (MQ=255)
                                                                                                                  aGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGa                                <  2:1483183/150‑1 (MQ=255)
                                                                                                                       cttcCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGt                           >  1:1096324/1‑150 (MQ=255)
                                                                                                                          ccggCCAGTCGGTCGGGATCAGATTAATCCGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTAtt                        <  1:3951478/147‑1 (MQ=255)
                                                                                                                          ccggCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTAtt                        <  1:3017278/147‑1 (MQ=255)
                                                                                                                             gCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCt                     >  1:1036761/1‑150 (MQ=255)
                                                                                                                               cAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGc                   <  1:2975225/150‑1 (MQ=255)
                                                                                                                               cAGTCGGTCGGGATCAGATTAATCAGCCGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTACCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGc                   >  1:1683605/1‑150 (MQ=255)
                                                                                                                                  tcggtcggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGa                >  2:350679/1‑150 (MQ=255)
                                                                                                                                  tcggtcggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGa                >  2:4020062/1‑150 (MQ=255)
                                                                                                                                   cggtcggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGAc               >  1:2511708/1‑150 (MQ=255)
                                                                                                                                    ggtcggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACt              >  1:1173953/1‑150 (MQ=255)
                                                                                                                                      tcggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgt            <  2:1110319/150‑1 (MQ=255)
                                                                                                                                         ggATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtagt         >  1:3446054/1‑150 (MQ=255)
                                                                                                                                          gATCAGATTACTCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCATTTTTCCGTGCGCTCCCGCCACATCACGGGTATGGCAAACGACAGGTATTCCTGCGGACTgtagta        <  2:532475/150‑1 (MQ=255)
                                                                                                                                             cAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc     <  2:316270/150‑1 (MQ=255)
                                                                                                                                               gATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGc   <  2:4389649/150‑1 (MQ=255)
                                                                                                                                                aTTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGct  >  2:282928/1‑149 (MQ=255)
                                                                                                                                                aTTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGcc  >  1:3375479/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCC  >  CP009273/3679177‑3679470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑