Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,740,770 C→A 14.4% I109I (ATC→ATA)  lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,740,7700CA14.4% 1036.7 / 98.5 305I109I (ATC→ATA) lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base C (134/127);  new base A (23/21);  total (157/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 326 total aligned reads displayed.

CGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGA  >  CP009273/3740621‑3740918
                                                                                                                                                     |                                                                                                                                                    
cgcgCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtc                                                                                                                                                      <  2:3335058/150‑1 (MQ=255)
 gcgcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcg                                                                                                                                                     >  1:2621132/1‑150 (MQ=255)
 gcgcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcg                                                                                                                                                     <  1:3998943/150‑1 (MQ=255)
 gcgcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcg                                                                                                                                                     <  1:4154978/150‑1 (MQ=255)
    cccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttc                                                                                                                                                  <  1:4405893/150‑1 (MQ=255)
    cccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttc                                                                                                                                                  <  2:2901967/150‑1 (MQ=255)
      cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttcgt                                                                                                                                                >  2:4557742/1‑150 (MQ=255)
      cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtagttcgt                                                                                                                                                >  2:701560/1‑150 (MQ=255)
       tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTtcgtc                                                                                                                                               <  2:1836313/150‑1 (MQ=255)
        gCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTtcgtcg                                                                                                                                              <  1:963755/150‑1 (MQ=255)
          ttACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCt                                                                                                                                            >  1:1429341/1‑150 (MQ=255)
           tACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTg                                                                                                                                           >  2:3928784/1‑150 (MQ=255)
           tACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTg                                                                                                                                           <  2:3370799/150‑1 (MQ=255)
                aTTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAgg                                                                                                                                      >  1:3159801/1‑150 (MQ=255)
                 ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAATGGGCTTGTTTTGGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGa                                                                                                                                     <  1:2325593/150‑1 (MQ=255)
                   cTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAg                                                                                                                                   <  2:2131633/150‑1 (MQ=255)
                   cTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAg                                                                                                                                   >  1:3431853/1‑150 (MQ=255)
                    tGGTGTTAGCGGGGAACAACTTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGa                                                                                                                                  <  2:4358550/150‑1 (MQ=255)
                    tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGa                                                                                                                                  <  2:1136167/150‑1 (MQ=255)
                     gttgtTAGCGGGTAACAAATTTTCGTTATCTGAATCTCCTCACAGGTAATGGGGTTTTTTTTGCTTGATAAAAACTACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCATGAAGAt                                                                                                                                 <  1:899058/148‑1 (MQ=255)
                     ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGAt                                                                                                                                 <  2:4266337/150‑1 (MQ=255)
                     ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGAt                                                                                                                                 >  2:4307977/1‑150 (MQ=255)
                      gtgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATg                                                                                                                                <  1:1829525/150‑1 (MQ=255)
                      gtgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATg                                                                                                                                <  1:85801/150‑1 (MQ=255)
                         ttAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCa                                                                                                                             <  2:2560166/150‑1 (MQ=255)
                          tAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCAt                                                                                                                            <  1:1024114/150‑1 (MQ=255)
                           aGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATc                                                                                                                           >  1:3294942/1‑150 (MQ=255)
                              ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCg                                                                                                                        <  1:3758389/150‑1 (MQ=255)
                              ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCg                                                                                                                        <  1:2565893/150‑1 (MQ=255)
                                   aCAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaa                                                                                                                   <  2:1991340/150‑1 (MQ=255)
                                     aaaTTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAAc                                                                                                                 >  2:2646239/1‑150 (MQ=255)
                                      aaTTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAAct                                                                                                                >  2:4020846/1‑150 (MQ=255)
                                       aTTGTCGGTATCGGCATCCCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAActc                                                                                                               >  2:4108419/1‑150 (MQ=255)
                                         tGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTa                                                                                                             <  1:740541/150‑1 (MQ=255)
                                           tCGGTATCGGCATCTCCGCACAGGGAAATGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTAcc                                                                                                           <  1:4536880/150‑1 (MQ=255)
                                            cGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTAccc                                                                                                          <  2:2797252/150‑1 (MQ=255)
                                             ggTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACACACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCg                                                                                                         <  1:1679855/150‑1 (MQ=255)
                                               tATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCt                                                                                                       <  1:3380445/150‑1 (MQ=255)
                                               tATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCt                                                                                                       >  1:845182/1‑150 (MQ=255)
                                                  cGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGAc                                                                                                    >  1:58681/1‑150 (MQ=255)
                                                   ggCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGAcc                                                                                                   >  2:837471/1‑150 (MQ=255)
                                                        ctcCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACa                                                                                              >  2:1963024/1‑150 (MQ=255)
                                                         tcCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACaa                                                                                             >  2:3153827/1‑150 (MQ=255)
                                                           cGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAAc                                                                                           >  2:1438882/1‑150 (MQ=255)
                                                            gCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAAcc                                                                                          >  1:2688297/1‑150 (MQ=255)
                                                            gCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAAcc                                                                                          >  2:1893470/1‑150 (MQ=255)
                                                             cacaGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCt                                                                                         >  1:1824994/1‑150 (MQ=255)
                                                              acaGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCtt                                                                                        >  2:1904827/1‑150 (MQ=255)
                                                              acaGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCtt                                                                                        >  1:1326677/1‑150 (MQ=255)
                                                              acaGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCtt                                                                                        >  2:3315148/1‑150 (MQ=255)
                                                                 gggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTtgtg                                                                                     >  1:2008060/1‑150 (MQ=255)
                                                                 gggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTtgtg                                                                                     <  2:2763831/150‑1 (MQ=255)
                                                                 gggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTtgtg                                                                                     <  2:759544/150‑1 (MQ=255)
                                                                   gAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGa                                                                                   >  1:1058747/1‑150 (MQ=255)
                                                                        ggCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCggg                                                                              >  1:1659512/1‑150 (MQ=255)
                                                                        ggCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCggg                                                                              <  1:4250731/150‑1 (MQ=255)
                                                                           ttGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCAt                                                                           <  1:4452429/150‑1 (MQ=255)
                                                                               ttttGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCgg                                                                       <  1:1723891/150‑1 (MQ=255)
                                                                                   gCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTc                                                                   <  1:1140333/150‑1 (MQ=255)
                                                                                   gCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTc                                                                   <  2:2363933/150‑1 (MQ=255)
                                                                                   gCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTc                                                                   >  2:1690450/1‑150 (MQ=255)
                                                                                     tGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGc                                                                 >  1:1207513/1‑150 (MQ=255)
                                                                                     tGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGc                                                                 >  1:3623269/1‑150 (MQ=255)
                                                                                       gATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTg                                                               >  2:3563439/1‑150 (MQ=255)
                                                                                         tAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGtt                                                             <  2:1319437/150‑1 (MQ=255)
                                                                                          aaaaaCGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTa                                                            >  2:1250710/1‑150 (MQ=255)
                                                                                          aaaaaCGACAAACAGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTa                                                            <  2:1086545/150‑1 (MQ=255)
                                                                                           aaaaCGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTAc                                                           <  1:1496997/150‑1 (MQ=255)
                                                                                            aaaCGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACg                                                          >  1:292440/1‑150 (MQ=255)
                                                                                             aaCGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgc                                                         >  2:2217867/1‑150 (MQ=255)
                                                                                               cGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctg                                                       >  2:2368233/1‑150 (MQ=255)
                                                                                                gACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctgg                                                      >  1:2442871/1‑150 (MQ=255)
                                                                                                gACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctgg                                                      >  1:2183131/1‑150 (MQ=255)
                                                                                                 aCAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggc                                                     >  2:4213805/1‑150 (MQ=255)
                                                                                                   aaaCCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggctg                                                   >  1:3313673/1‑150 (MQ=255)
                                                                                                     aCCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGaa                                                 >  2:3681638/1‑150 (MQ=255)
                                                                                                         cTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAagag                                             >  2:532544/1‑150 (MQ=255)
                                                                                                          tCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGc                                            >  2:661191/1‑150 (MQ=255)
                                                                                                            gggAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCAc                                          <  1:1438037/150‑1 (MQ=255)
                                                                                                             ggAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACg                                         >  1:609846/1‑150 (MQ=255)
                                                                                                              taaTGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCATGAAGATGGCATCCATGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTTTTACGCTGGCTGAAATAGCACGa                                        <  2:2084578/149‑1 (MQ=255)
                                                                                                               aaTGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGaa                                       >  1:4045938/1‑150 (MQ=255)
                                                                                                                aTGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAAc                                      <  1:2981033/150‑1 (MQ=255)
                                                                                                                  gCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCa                                    <  1:63474/150‑1 (MQ=255)
                                                                                                                   cTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAg                                   >  1:1286160/1‑150 (MQ=255)
                                                                                                                     aTTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGaa                                 <  1:2156241/150‑1 (MQ=255)
                                                                                                                     aTTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGaa                                 >  2:1359486/1‑150 (MQ=255)
                                                                                                                      ttttGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAAc                                <  1:1867321/150‑1 (MQ=255)
                                                                                                                      ttttGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAAc                                <  1:991530/150‑1 (MQ=255)
                                                                                                                       tttGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACg                               >  2:2227950/1‑150 (MQ=255)
                                                                                                                           tCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTAc                           <  1:1835502/150‑1 (MQ=255)
                                                                                                                                         ggCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCTGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACTCGCAAATTGGCTg             <  2:1491294/150‑1 (MQ=255)
                                                                                                                                               ggAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgat       <  1:3927614/150‑1 (MQ=255)
                                                                                                                                               ggAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgat       >  1:4414402/1‑150 (MQ=255)
                                                                                                                                               ggAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgat       >  2:2477132/1‑150 (MQ=255)
                                                                                                                                               ggAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgat       <  1:353357/150‑1 (MQ=255)
                                                                                                                                                gAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgatg      <  2:2290157/150‑1 (MQ=255)
                                                                                                                                                 aaaTAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgatga     <  2:4474704/150‑1 (MQ=255)
                                                                                                                                                 aaaTAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgatga     <  2:1746441/150‑1 (MQ=255)
                                                                                                                                                    tcgttcgtCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGtgatgatga  <  2:552738/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGCTACGCGCAAATTGGCTGCGTGATGATGA  >  CP009273/3740621‑3740918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑