BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,761,602	G→A	20.1%	V22V (GTG→GTA)	yibG →	TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,761,602	0	G	A	20.1%	610.3 / 140.2	258	V22V (GTG→GTA)	yibG	TPR‑like repeat protein
Reads supporting (aligned to +/- strand): ref base G (109/97); new base A (31/21); total (140/118)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.10e-02

Only 100 of 262 total aligned reads displayed.

AACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTAAAAA  >  CP009273/3761453‑3761751

aaCATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGAtgtg                                                                                                                                                       >  2:1955139/1‑150 (MQ=255)

   atatGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGaaa                                                                                                                                                    <  2:1545778/150‑1 (MQ=255)

     atGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAAt                                                                                                                                                  <  1:1593739/150‑1 (MQ=255)

      tGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATa                                                                                                                                                 <  1:350289/150‑1 (MQ=255)

       gggCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaa                                                                                                                                                >  1:3818159/1‑150 (MQ=255)

           cATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATaaaaca                                                                                                                                            >  2:658847/1‑150 (MQ=255)

            aTCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaa                                                                                                                                           >  1:2116669/1‑150 (MQ=255)

               tGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaaaac                                                                                                                                        >  2:16189/1‑150 (MQ=255)

                gggCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACg                                                                                                                                       >  1:3406310/1‑150 (MQ=255)

                gggCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACg                                                                                                                                       >  1:3447688/1‑150 (MQ=255)

                  gCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGaa                                                                                                                                     >  2:2657104/1‑150 (MQ=255)

                    aCCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAg                                                                                                                                   >  1:4446564/1‑150 (MQ=255)

                        cATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTatg                                                                                                                               <  1:3751559/150‑1 (MQ=255)

                          tttCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTatgat                                                                                                                             <  2:2356176/150‑1 (MQ=255)

                          tttCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTatgat                                                                                                                             <  2:296734/150‑1 (MQ=255)

                             cTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATggg                                                                                                                          <  1:596783/150‑1 (MQ=255)

                                atatGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTc                                                                                                                       <  2:3480392/150‑1 (MQ=255)

                                atatGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTc                                                                                                                       >  2:2733459/1‑150 (MQ=255)

                                 tatGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCt                                                                                                                      >  1:4069142/1‑150 (MQ=255)

                                 tatGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCt                                                                                                                      >  1:1311147/1‑150 (MQ=255)

                                  atGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTa                                                                                                                     >  2:1781292/1‑150 (MQ=255)

                                  atGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTa                                                                                                                     <  1:3200191/150‑1 (MQ=255)

                                   tGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAc                                                                                                                    <  2:4185739/150‑1 (MQ=255)

                                    gCAAGTTACGCCAGAAAAACCCAGTTATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTAAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACa                                                                                                                   <  1:2149856/150‑1 (MQ=255)

                                      aaGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGc                                                                                                                 <  2:3515904/150‑1 (MQ=255)

                                       aGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCa                                                                                                                >  2:326366/1‑150 (MQ=255)

                                       aGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCa                                                                                                                <  1:2685558/150‑1 (MQ=255)

                                           aCGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGa                                                                                                            <  2:3592956/150‑1 (MQ=255)

                                           aCGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGa                                                                                                            >  1:2718616/1‑150 (MQ=255)

                                            cGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGAc                                                                                                           >  2:2781796/1‑150 (MQ=255)

                                             gCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAGATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGAcc                                                                                                          >  2:2336418/1‑150 (MQ=255)

                                             gCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGAcc                                                                                                          >  2:3113599/1‑150 (MQ=255)

                                               cAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTa                                                                                                        >  2:2918683/1‑150 (MQ=255)

                                                aGAAAAACCCATTGATATAAGATTGGTTGTTGATTTTTGATAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCATTTGCATGGTGCTTATGTGAAAATAAAACAAAACGATAGTATGATGGGGTCTACAGCAATGTCCTAt                                                                                                       <  2:4453609/150‑1 (MQ=255)

                                                aGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTAt                                                                                                       >  2:1370405/1‑150 (MQ=255)

                                                aGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTAt                                                                                                       >  1:552647/1‑150 (MQ=255)

                                                    aaaCCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGAtt                                                                                                   >  2:3884916/1‑150 (MQ=255)

                                                        ccAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAg                                                                                               <  1:1267454/150‑1 (MQ=255)

                                                          aGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTg                                                                                             <  2:627704/150‑1 (MQ=255)

                                                           gTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTga                                                                                            <  1:25242/150‑1 (MQ=255)

                                                             gATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTgaag                                                                                          <  2:3925762/150‑1 (MQ=255)

                                                                ataAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑ATTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGaaa                                                                                       >  1:2248776/1‑150 (MQ=255)

                                                                  aaGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAg                                                                                     >  2:2612501/1‑150 (MQ=255)

                                                                  aaGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAg                                                                                     <  1:3836062/150‑1 (MQ=255)

                                                                       aGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTtgat                                                                                <  2:1196786/150‑1 (MQ=255)

                                                                       aGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGATGATTTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGAAATGACCTATGATTTAAGTGAAGAAAAGTtgat                                                                                >  1:4525349/1‑150 (MQ=255)

                                                                        ggTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTtgatg                                                                               <  2:1997550/150‑1 (MQ=255)

                                                                            gTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAAc                                                                           >  1:3063520/1‑150 (MQ=255)

                                                                               gATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGa                                                                        <  1:2648397/150‑1 (MQ=255)

                                                                               gATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGa                                                                        <  1:1446783/150‑1 (MQ=255)

                                                                               gATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGa                                                                        <  1:2648353/150‑1 (MQ=255)

                                                                                aTGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGaa                                                                       >  1:1822814/1‑150 (MQ=255)

                                                                                    aTGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGtat                                                                   <  1:1973143/150‑1 (MQ=255)

                                                                                     tGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGtata                                                                  >  2:4416065/1‑150 (MQ=255)

                                                                                      gAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATaa                                                                 >  1:1255025/1‑150 (MQ=255)

                                                                                        aaGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCACTGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAAt                                                               >  1:961673/1‑150 (MQ=255)

                                                                                        aaGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAAt                                                               >  1:2417178/1‑150 (MQ=255)

                                                                                        aaGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAAt                                                               >  1:1255676/1‑150 (MQ=255)

                                                                                        aaGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAAt                                                               >  2:3569117/1‑150 (MQ=255)

                                                                                          gCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATca                                                             >  1:3090030/1‑150 (MQ=255)

                                                                                           cATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATcac                                                            >  1:993121/1‑150 (MQ=255)

                                                                                                ttGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACAt                                                       >  1:1196459/1‑150 (MQ=255)

                                                                                                 tGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATg                                                      >  1:284192/1‑150 (MQ=255)

                                                                                                  gTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATgg                                                     >  1:718181/1‑150 (MQ=255)

                                                                                                   ttACTATTTTTTTTATTTCTATTTTATTTGTCAATTGCATGGTGATTATGTGAAAATAAAAAAAAACTAAGGTTTGATGGGGACTAAAGCAATGACCTATTATTTAAGTGAAGAAAAGTTGATGAAAAGGAAGTATAAATCAAAACATgg                                                     >  2:862520/1‑150 (MQ=255)

                                                                                                   ttACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGt                                                    >  1:3930340/1‑150 (MQ=255)

                                                                                                    tACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTg                                                   >  1:862651/1‑150 (MQ=255)

                                                                                                     aCTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGa                                                  >  2:2024953/1‑150 (MQ=255)

                                                                                                     aCTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGa                                                  >  1:891000/1‑150 (MQ=255)

                                                                                                     aCTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGa                                                  >  1:148020/1‑150 (MQ=255)

                                                                                                      cTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGAt                                                 <  2:750738/150‑1 (MQ=255)

                                                                                                      cTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGAt                                                 >  2:226862/1‑150 (MQ=255)

                                                                                                           ttttttATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCag                                             <  2:82784/150‑1 (MQ=255)

                                                                                                             ttttCTTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGACACTGAAGTATAAATCACAACATGGTGATTCagag                                           <  1:2060897/150‑1 (MQ=255)

                                                                                                              tttATTTCTCTTTTATTTGTCAATTTCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAgg                                          <  2:409518/150‑1 (MQ=255)

                                                                                                                 aTTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcat                                       <  1:4528682/150‑1 (MQ=255)

                                                                                                                 aTTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcat                                       >  2:1436652/1‑150 (MQ=255)

                                                                                                                 aTTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcat                                       >  2:2984445/1‑150 (MQ=255)

                                                                                                                 aTTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcat                                       >  2:1277216/1‑150 (MQ=255)

                                                                                                                   ttCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcatca                                     >  1:4145272/1‑150 (MQ=255)

                                                                                                                    tctcTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcatcat                                    <  2:2913129/150‑1 (MQ=255)

                                                                                                                       ctTTTATTTGTCAAGTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTc                                 <  1:2978329/150‑1 (MQ=255)

                                                                                                                        ttttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCg                                >  2:521271/1‑150 (MQ=255)

                                                                                                                          ttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGAc                              <  2:1862180/150‑1 (MQ=255)

                                                                                                                           tATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACt                             <  2:781376/150‑1 (MQ=255)

                                                                                                                           tATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACt                             <  2:2873397/150‑1 (MQ=255)

                                                                                                                           tATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACt                             <  2:2873507/150‑1 (MQ=255)

                                                                                                                             tttGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACtat                           <  2:2123123/150‑1 (MQ=255)

                                                                                                                                   aaTTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAat                     >  1:1577296/1‑150 (MQ=255)

                                                                                                                                     ttGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAatat                   >  1:1920948/1‑150 (MQ=255)

                                                                                                                                       gCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATACAGAGGCATCATTTCGACTATATCAATATTa                 >  2:4159424/1‑150 (MQ=255)

                                                                                                                                         aTGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACt               >  1:3666547/1‑150 (MQ=255)

                                                                                                                                           ggTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGc             >  2:4510062/1‑150 (MQ=255)

                                                                                                                                            gTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCt            <  1:171533/150‑1 (MQ=255)

                                                                                                                                              gCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCttt          <  2:1895735/150‑1 (MQ=255)

                                                                                                                                              gCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCttt          <  1:1614986/150‑1 (MQ=255)

                                                                                                                                                tGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTAc        <  2:515895/150‑1 (MQ=255)

                                                                                                                                                  aTGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTa      <  2:3032402/150‑1 (MQ=255)

                                                                                                                                                     tgAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTaaaa   >  1:1727736/1‑150 (MQ=255)

                                                                                                                                                      gAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTaaaaa  <  1:326366/150‑1 (MQ=255)

AACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTA‑TTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTAAAAA  >  CP009273/3761453‑3761751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`