Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,810,195 C→A 100% R54S (CGT→AGT)  yicC → UPF0701 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,810,1950CA97.4% 818.4 / 9.0 245R54S (CGT→AGT) yicCUPF0701 family protein
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (128/110);  total (130/115)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 245 total aligned reads displayed.

CCGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTCGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAGGG  >  CP009273/3810052‑3810341
                                                                                                                                               |                                                                                                                                                  
ccGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTtctc                                                                                                                                              <  2:3152863/150‑1 (MQ=255)
 cGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCg                                                                                                                                             >  1:1932669/1‑150 (MQ=255)
        gCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGAcg                                                                                                                                      <  1:584785/150‑1 (MQ=255)
         cccGGCGTGAAATCCAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTGATCTGGAAACTTACTTTCGGCTGCCGGAGCAGTGCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTCTTAGTTCTCGCCTGAcgc                                                                                                                                     >  2:791560/1‑150 (MQ=255)
         cccGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGAcgc                                                                                                                                     >  1:3772599/1‑150 (MQ=255)
          ccGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGAcgcg                                                                                                                                    >  2:2487779/1‑150 (MQ=255)
          ccGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGAcgcg                                                                                                                                    >  1:3680229/1‑150 (MQ=255)
           cGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGAcgcgc                                                                                                                                   >  1:1201878/1‑150 (MQ=255)
             gCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCgg                                                                                                                                 <  2:3627003/150‑1 (MQ=255)
               gTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTa                                                                                                                               >  2:3040475/1‑150 (MQ=255)
               gTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTa                                                                                                                               <  2:183449/150‑1 (MQ=255)
                    aTCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTg                                                                                                                          >  1:1780847/1‑150 (MQ=255)
                    aTCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTg                                                                                                                          >  1:3973802/1‑150 (MQ=255)
                     tCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTgg                                                                                                                         >  2:1733059/1‑150 (MQ=255)
                      cAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGa                                                                                                                        <  2:824598/150‑1 (MQ=255)
                       aaGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGaa                                                                                                                       >  1:1517812/1‑150 (MQ=255)
                        aGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTCGTTCTCGCCTGACGCGCGGTAAAGTGGAAt                                                                                                                      <  2:3933775/150‑1 (MQ=255)
                           gTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTa                                                                                                                   >  1:4116115/1‑150 (MQ=255)
                             gAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTAcc                                                                                                                 >  2:3954654/1‑150 (MQ=255)
                             gAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTAcc                                                                                                                 >  2:424519/1‑150 (MQ=255)
                             gAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTAcc                                                                                                                 >  2:1117029/1‑150 (MQ=255)
                               aTGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCt                                                                                                               <  2:1958472/150‑1 (MQ=255)
                               aTGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCt                                                                                                               <  2:1546313/150‑1 (MQ=255)
                                  ggggAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTgcg                                                                                                            <  2:2173354/150‑1 (MQ=255)
                                  ggggAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTgcg                                                                                                            <  2:2173316/150‑1 (MQ=255)
                                     gAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTa                                                                                                         <  2:3577239/150‑1 (MQ=255)
                                     gAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTa                                                                                                         >  2:4079098/1‑150 (MQ=255)
                                     gAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTa                                                                                                         >  2:4230223/1‑150 (MQ=255)
                                           aaCCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGcc                                                                                                   <  2:3821323/150‑1 (MQ=255)
                                               tGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGAt                                                                                               >  2:915314/1‑150 (MQ=255)
                                                gggAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATg                                                                                              >  2:1452483/1‑150 (MQ=255)
                                                 ggAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGt                                                                                             >  2:2663193/1‑150 (MQ=255)
                                                  gAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGtt                                                                                            <  1:2724443/150‑1 (MQ=255)
                                                   aaaTGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTa                                                                                           >  2:3330920/1‑150 (MQ=255)
                                                   aaaTGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTa                                                                                           >  2:2313297/1‑150 (MQ=255)
                                                              gTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGt                                                                                <  2:384614/150‑1 (MQ=255)
                                                              gTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGt                                                                                >  2:1036892/1‑150 (MQ=255)
                                                                aaaCCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGa                                                                              >  2:3229925/1‑150 (MQ=255)
                                                                 aaCCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAg                                                                             <  2:4301689/150‑1 (MQ=255)
                                                                 aaCCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAg                                                                             <  1:1023344/150‑1 (MQ=255)
                                                                  aCCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGc                                                                            <  2:594052/150‑1 (MQ=255)
                                                                   ccAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCt                                                                           <  2:941158/150‑1 (MQ=255)
                                                                    cAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTg                                                                          <  1:875676/150‑1 (MQ=255)
                                                                    cAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTg                                                                          >  1:4448379/1‑150 (MQ=255)
                                                                    cAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTg                                                                          >  2:2380002/1‑150 (MQ=255)
                                                                    cAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTg                                                                          >  1:3978637/1‑150 (MQ=255)
                                                                         ttATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCt                                                                     <  1:3254904/150‑1 (MQ=255)
                                                                         ttATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCt                                                                     >  2:649243/1‑150 (MQ=255)
                                                                         ttATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCt                                                                     <  1:2792762/150‑1 (MQ=255)
                                                                          tATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTc                                                                    <  2:1211143/150‑1 (MQ=255)
                                                                          tATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTc                                                                    <  1:1649431/150‑1 (MQ=255)
                                                                           aTCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCa                                                                   <  1:3477017/150‑1 (MQ=255)
                                                                              tGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACg                                                                >  2:859093/1‑150 (MQ=255)
                                                                               ggAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGa                                                               >  1:551915/1‑150 (MQ=255)
                                                                                gtaaCTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGaa                                                              <  1:4094694/148‑1 (MQ=255)
                                                                                 aaaCTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGaaa                                                             >  1:1491993/1‑150 (MQ=255)
                                                                                  aaCTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGaaaa                                                            >  2:2372459/1‑150 (MQ=255)
                                                                                    cttacttTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAAc                                                          >  2:1540656/1‑150 (MQ=255)
                                                                                      tacttTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTg                                                        <  2:577088/150‑1 (MQ=255)
                                                                                         tttCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCt                                                     <  2:1491993/150‑1 (MQ=255)
                                                                                         tttCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCt                                                     <  2:2884197/150‑1 (MQ=255)
                                                                                          ttCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTa                                                    >  1:2670459/1‑150 (MQ=255)
                                                                                             gTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAAc                                                 <  2:620/150‑1 (MQ=255)
                                                                                                 gCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCt                                             <  1:2016836/150‑1 (MQ=255)
                                                                                                 gCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCt                                             <  2:3378946/150‑1 (MQ=255)
                                                                                                    ggAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGt                                          <  1:2395850/150‑1 (MQ=255)
                                                                                                    ggAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGt                                          <  1:1453943/150‑1 (MQ=255)
                                                                                                     gAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTa                                         >  2:1748787/1‑150 (MQ=255)
                                                                                                     gAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTa                                         <  1:3967265/150‑1 (MQ=255)
                                                                                                      aGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTaa                                        >  1:1212078/1‑150 (MQ=255)
                                                                                                      aGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGAGTATTAGTTCTCGCATTACGCGCGGTAAAGTGGAATGTAACCTGCGCTAATAGCCAGAAGTTATCGCGCAAGGTGAGCTGATCCTAAACGAAAAACTGGAGAAACAGCATGTaa                                        >  1:138492/1‑150 (MQ=255)
                                                                                                         aGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTg                                     <  1:3259355/150‑1 (MQ=255)
                                                                                                         aGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTg                                     <  1:258164/150‑1 (MQ=255)
                                                                                                         aGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTg                                     <  2:4181720/150‑1 (MQ=255)
                                                                                                              cGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCcgcg                                >  2:744837/1‑150 (MQ=255)
                                                                                                                   ccTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTg                           <  1:2634214/150‑1 (MQ=255)
                                                                                                                   ccTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTg                           >  2:397648/1‑150 (MQ=255)
                                                                                                                      tGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGt                        >  1:820070/1‑150 (MQ=255)
                                                                                                                       gcaCCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACACTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTa                       <  2:1427914/148‑1 (MQ=255)
                                                                                                                        aaCCTGTCGTTCGCGAGCGTATTCGTTCTCNCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTaa                      >  1:4280969/1‑150 (MQ=255)
                                                                                                                         aCCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTaaa                     >  1:2851449/1‑150 (MQ=255)
                                                                                                                         aCCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTaaa                     >  2:4407012/1‑150 (MQ=255)
                                                                                                                         aCCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTaaa                     <  2:3772599/150‑1 (MQ=255)
                                                                                                                              tCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGc                >  1:4161001/1‑150 (MQ=255)
                                                                                                                               cGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCa               >  2:3313343/1‑150 (MQ=255)
                                                                                                                                 ttCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCaga             >  2:2872000/1‑150 (MQ=255)
                                                                                                                                   cgcgAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGt           >  1:3883551/1‑150 (MQ=255)
                                                                                                                                    gcgAGCGTATTCGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTg          <  2:2385362/150‑1 (MQ=255)
                                                                                                                                    gcgAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTg          <  1:2343149/150‑1 (MQ=255)
                                                                                                                                    gcgAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTg          <  1:1414155/150‑1 (MQ=255)
                                                                                                                                     gtaGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGa         <  1:1166479/148‑1 (MQ=255)
                                                                                                                                      gAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGAc        >  2:2552305/1‑150 (MQ=255)
                                                                                                                                      gAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGAc        <  1:51044/150‑1 (MQ=255)
                                                                                                                                        gCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGa      <  1:4106914/150‑1 (MQ=255)
                                                                                                                                          gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCTCTATGAGCCAGATGTTAGAGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    <  1:3954654/150‑1 (MQ=255)
                                                                                                                                          gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    >  1:649440/1‑150 (MQ=255)
                                                                                                                                          gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    >  1:3065269/1‑150 (MQ=255)
                                                                                                                                           tATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAgg   >  1:1154953/1‑150 (MQ=255)
                                                                                                                                           tATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTACCGCGAACTGGGTAAAAATGCAGAGTGACGAAgg   >  2:3655603/1‑150 (MQ=255)
                                                                                                                                            aTTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAggg  >  1:3391727/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CCGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTCGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAGGG  >  CP009273/3810052‑3810341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑