Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,819,936 C→A 13.1% T456K (ACG→AAG)  recG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,819,9360CA13.1% 1111.6 / 89.9 319T456K (ACG→AAG) recGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (160/117);  new base A (14/28);  total (174/145)
Fisher's exact test for biased strand distribution p-value = 4.30e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 323 total aligned reads displayed.

GCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAA  >  CP009273/3819787‑3820084
                                                                                                                                                     |                                                                                                                                                    
gCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAc                                                                                                                                                      <  1:1042512/150‑1 (MQ=255)
gCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAc                                                                                                                                                      <  1:1786426/150‑1 (MQ=255)
 cGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACg                                                                                                                                                     >  2:1331810/1‑150 (MQ=255)
       gCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTg                                                                                                                                               <  1:4438163/150‑1 (MQ=255)
       gCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTg                                                                                                                                               <  1:2093471/150‑1 (MQ=255)
         aTTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTAGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGAc                                                                                                                                             <  1:3953739/150‑1 (MQ=255)
             tgGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACg                                                                                                                                         <  1:1971526/150‑1 (MQ=255)
              gggAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACgg                                                                                                                                        >  1:1822613/1‑150 (MQ=255)
               ggAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGt                                                                                                                                       >  2:342413/1‑150 (MQ=255)
                    aaGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTa                                                                                                                                  >  1:3566793/1‑150 (MQ=255)
                    aaGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTa                                                                                                                                  >  2:3541433/1‑150 (MQ=255)
                      ggCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACTCCGATCCCCCGCACGCTGGCAAAGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCAGCACGCCAGTGACTACGGTCGCTAtt                                                                                                                                >  2:2568238/1‑150 (MQ=255)
                          agcagcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTg                                                                                                                            <  1:4084200/150‑1 (MQ=255)
                             agcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATa                                                                                                                         >  2:3170746/1‑150 (MQ=255)
                             agcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATa                                                                                                                         >  2:4068606/1‑150 (MQ=255)
                             agcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATa                                                                                                                         >  2:4221704/1‑150 (MQ=255)
                                agGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATAccc                                                                                                                      >  1:4330743/1‑150 (MQ=255)
                                 gggCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACccg                                                                                                                     >  2:2737041/1‑150 (MQ=255)
                                    cTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCACTGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACccgccg                                                                                                                  >  2:3438959/1‑150 (MQ=255)
                                        cATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTAcc                                                                                                              <  2:2705800/150‑1 (MQ=255)
                                              cATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGAcatc                                                                                                        >  1:3715636/1‑150 (MQ=255)
                                                  aGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTg                                                                                                    >  2:1662341/1‑150 (MQ=255)
                                                   gTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGa                                                                                                   <  2:1757825/150‑1 (MQ=255)
                                                    ttGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGAc                                                                                                  <  1:691671/150‑1 (MQ=255)
                                                     tGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGAcc                                                                                                 <  1:2799981/150‑1 (MQ=255)
                                                     tGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGAcc                                                                                                 <  1:2167483/150‑1 (MQ=255)
                                                     tGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGAcc                                                                                                 >  1:2543958/1‑150 (MQ=255)
                                                      gATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACcg                                                                                                <  1:904266/150‑1 (MQ=255)
                                                        tCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACAACATTGACcgcg                                                                                              >  1:4217374/1‑150 (MQ=255)
                                                           tGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGc                                                                                           <  2:3990081/150‑1 (MQ=255)
                                                             aCCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcac                                                                                         >  2:3703920/1‑150 (MQ=255)
                                                             aCCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcac                                                                                         >  1:4576240/1‑150 (MQ=255)
                                                              ccGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcacc                                                                                        >  2:192178/1‑150 (MQ=255)
                                                               cGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcacca                                                                                       <  1:1346558/150‑1 (MQ=255)
                                                                gCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcaccac                                                                                      <  2:4215528/150‑1 (MQ=255)
                                                                  cACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGc                                                                                    >  1:584035/1‑150 (MQ=255)
                                                                      ccGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGc                                                                                <  1:3140322/150‑1 (MQ=255)
                                                                        gATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCAt                                                                              >  1:468270/1‑150 (MQ=255)
                                                                        gATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCAt                                                                              >  2:3778626/1‑150 (MQ=255)
                                                                         aTCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATa                                                                             <  2:290898/150‑1 (MQ=255)
                                                                          tCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATaa                                                                            >  2:2612505/1‑150 (MQ=255)
                                                                          tCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATaa                                                                            >  2:236833/1‑150 (MQ=255)
                                                                          tCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATaa                                                                            <  2:1969915/150‑1 (MQ=255)
                                                                                   cGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtc                                                                   <  1:556681/150‑1 (MQ=255)
                                                                                   cGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtc                                                                   <  1:3114896/150‑1 (MQ=255)
                                                                                    gCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtcg                                                                  >  2:470017/1‑150 (MQ=255)
                                                                                      tGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGAAAGATGAGCTGCCGCCAGGCCGCACGCCAGTTACTACGTTCGCTATTCCTGATACCCGCCGGACCGACATCAATGACCGCGTGCACCACGCCTTCATAACTGAAGgtcgtc                                                                >  1:1591740/1‑150 (MQ=255)
                                                                                        gCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAg                                                              <  1:3713082/150‑1 (MQ=255)
                                                                                           aTGACTGCGTATGCCGATCTCGATACCTCGGTGATTGTTGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCa                                                           <  1:4503350/150‑1 (MQ=255)
                                                                                            tGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCAt                                                          <  2:3342594/150‑1 (MQ=255)
                                                                                             gACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATa                                                         >  1:3530620/1‑150 (MQ=255)
                                                                                              aCTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATAc                                                        <  2:718065/150‑1 (MQ=255)
                                                                                               cTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACt                                                       <  1:342413/150‑1 (MQ=255)
                                                                                                tGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTAATCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTg                                                      >  1:1541459/1‑150 (MQ=255)
                                                                                                 gagaaTGCAGATCTCGATACCTGGTTTATTTATGAGCTGCCGCCAGTCGGCACGCAAGTGACTACGGTCGCTATTCCTGTTACCCTACGTACCGACATCCTTGACCGCGTGCACCACGCCTGCTTAACTGAAGGTCGTCAGGCTTTCTgg                                                     <  2:577753/146‑1 (MQ=255)
                                                                                                    tATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGtt                                                  >  1:1269997/1‑150 (MQ=255)
                                                                                                    tATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGtt                                                  >  2:1749665/1‑150 (MQ=255)
                                                                                                    tATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTAATCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGtt                                                  >  1:2420699/1‑150 (MQ=255)
                                                                                                     aTGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGttt                                                 <  2:3031642/150‑1 (MQ=255)
                                                                                                         cGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTAc                                             <  1:991952/150‑1 (MQ=255)
                                                                                                         cGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACAGGGTTTGTAc                                             >  1:2678028/1‑150 (MQ=255)
                                                                                                          gATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACg                                            >  2:1425225/1‑150 (MQ=255)
                                                                                                           aTCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGt                                           <  2:2253931/150‑1 (MQ=255)
                                                                                                           aTCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGt                                           <  1:4391318/150‑1 (MQ=255)
                                                                                                            tctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGtt                                          <  2:1341113/150‑1 (MQ=255)
                                                                                                            tctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGtt                                          >  2:3475633/1‑150 (MQ=255)
                                                                                                              tcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttga                                        >  1:1222382/1‑150 (MQ=255)
                                                                                                                gATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgatt                                      >  2:1351002/1‑150 (MQ=255)
                                                                                                                gATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgatt                                      >  1:679088/1‑150 (MQ=255)
                                                                                                                gATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgatt                                      <  1:1085006/150‑1 (MQ=255)
                                                                                                                 aTACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgattg                                     <  2:4443393/150‑1 (MQ=255)
                                                                                                                 aTACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgattg                                     >  2:3078659/1‑150 (MQ=255)
                                                                                                                  tACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgattga                                    >  2:3246767/1‑150 (MQ=255)
                                                                                                                   aCCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGaa                                   <  1:2271200/150‑1 (MQ=255)
                                                                                                                   aCCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGaa                                   >  1:1867366/1‑150 (MQ=255)
                                                                                                                   aCCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACAGCGTTCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGaa                                   <  1:4605358/150‑1 (MQ=255)
                                                                                                                    ccTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAag                                  >  2:875802/1‑150 (MQ=255)
                                                                                                                    ccTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAag                                  <  1:2069426/150‑1 (MQ=255)
                                                                                                                      tCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCAGATACCCGCCGTACCGACCTCATTGACCGCGTGCACCACGCCTGCAAAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAagag                                >  1:4239372/1‑150 (MQ=255)
                                                                                                                       cGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGt                               <  2:1455866/150‑1 (MQ=255)
                                                                                                                        ggTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTc                              >  1:3765823/1‑150 (MQ=255)
                                                                                                                          tGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCgg                            <  1:626673/150‑1 (MQ=255)
                                                                                                                           gATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGa                           >  2:1285523/1‑150 (MQ=255)
                                                                                                                             tAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAt                         <  1:768559/150‑1 (MQ=255)
                                                                                                                             tAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAt                         <  1:517796/150‑1 (MQ=255)
                                                                                                                              aGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAtt                        <  1:3475967/150‑1 (MQ=255)
                                                                                                                              aGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAtt                        >  1:2968053/1‑150 (MQ=255)
                                                                                                                                aTGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGc                      >  1:2898211/1‑150 (MQ=255)
                                                                                                                                aTGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGc                      <  2:3806754/150‑1 (MQ=255)
                                                                                                                                aTGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGGTTGTACGTTGATTGAAGAGTCGGAATTGc                      >  1:1747062/1‑150 (MQ=255)
                                                                                                                                  gAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTg                    <  1:1464593/150‑1 (MQ=255)
                                                                                                                                   aGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTgg                   <  1:2573216/150‑1 (MQ=255)
                                                                                                                                       gccgccAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAgc               >  1:379304/1‑150 (MQ=255)
                                                                                                                                        ccgccAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGTAATTGCTGGAAgcg              <  2:4071573/150‑1 (MQ=255)
                                                                                                                                          gccAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCa            >  2:310948/1‑150 (MQ=255)
                                                                                                                                          gccAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCa            >  1:3868114/1‑150 (MQ=255)
                                                                                                                                            cAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAgg          <  1:862883/150‑1 (MQ=255)
                                                                                                                                            cAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAgg          >  2:771448/1‑150 (MQ=255)
                                                                                                                                              ggCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggcg        <  2:3937097/150‑1 (MQ=255)
                                                                                                                                                    aCGCCAGTGACTACGGTCGCTATTACTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCAGCATAACTGTAGGTCGTCAGGCCTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGaa  >  1:1194180/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAA  >  CP009273/3819787‑3820084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑