BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,829,020	G→T	59.0%	F90L (TTC→TTA)	yicJ ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,829,020	0	G	T	59.0%	59.7 / inf	310	F90L (TTC→TTA)	yicJ	putative transporter
Reads supporting (aligned to +/- strand): ref base G (70/57); new base T (102/80); total (173/137)
Fisher's exact test for biased strand distribution p-value = 9.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 311 total aligned reads displayed.

CATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCC  >  CP009273/3828872‑3829169

cATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGa                                                                                                                                                      >  1:1327757/1‑150 (MQ=255)

    ggTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATgg                                                                                                                                                  <  2:494309/150‑1 (MQ=255)

      tGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCa                                                                                                                                                >  2:861187/1‑150 (MQ=255)

          tACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTgc                                                                                                                                            <  1:3639495/150‑1 (MQ=255)

           acacCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTgcg                                                                                                                                           <  1:743554/150‑1 (MQ=255)

            caccacCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTgcgc                                                                                                                                          >  2:437049/1‑150 (MQ=255)

              ccaccCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCa                                                                                                                                        <  2:1593075/150‑1 (MQ=255)

                    aaTGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGt                                                                                                                                  <  2:4401084/150‑1 (MQ=255)

                      tGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTAc                                                                                                                                <  2:4088058/150‑1 (MQ=255)

                      tGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTAc                                                                                                                                >  1:1752690/1‑150 (MQ=255)

                         gcAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCa                                                                                                                             <  1:2154652/150‑1 (MQ=255)

                          cAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAAATGCTGCATAGATCATTTTGCCGTTCATACTTAGATCTGGCGAGCTATAGTCCAGAACACAGACGAACCCTAATGGCAGTGCGCCAAACAGTACCCAc                                                                                                                            >  2:3072054/1‑150 (MQ=255)

                              aaGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGAc                                                                                                                        <  1:4361074/150‑1 (MQ=255)

                              aaGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGAc                                                                                                                        >  2:106451/1‑150 (MQ=255)

                                    tatTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAAtt                                                                                                                  <  2:704802/150‑1 (MQ=255)

                                        gacgacGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTAcc                                                                                                              <  2:2125706/150‑1 (MQ=255)

                                           gacgGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCa                                                                                                           <  1:3176748/150‑1 (MQ=255)

                                           gacgGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCa                                                                                                           <  2:776836/150‑1 (MQ=255)

                                             cgGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGc                                                                                                         >  1:4037276/1‑150 (MQ=255)

                                              ggcataAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCg                                                                                                        <  1:685474/147‑1 (MQ=255)

                                              ggTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCg                                                                                                        >  2:1225057/1‑150 (MQ=255)

                                               gTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCga                                                                                                       <  1:1127931/150‑1 (MQ=255)

                                                 ataAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCgaga                                                                                                     <  1:3128497/150‑1 (MQ=255)

                                                     aGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAgcgc                                                                                                 >  2:2687870/1‑150 (MQ=255)

                                                       taaggtaagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGt                                                                                               >  1:3521977/1‑150 (MQ=255)

                                                        aaggtaagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGtt                                                                                              >  2:3035019/1‑150 (MQ=255)

                                                         aggtaagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTc                                                                                             >  2:1843306/1‑150 (MQ=255)

                                                         aggtaagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTc                                                                                             <  1:3932696/150‑1 (MQ=255)

                                                          ggtaagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCg                                                                                            <  1:2945563/150‑1 (MQ=255)

                                                            taagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGAt                                                                                          <  2:1931524/150‑1 (MQ=255)

                                                            taagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGAt                                                                                          >  1:3246163/1‑150 (MQ=255)

                                                            taagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGAt                                                                                          <  2:3649674/150‑1 (MQ=255)

                                                             aagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATc                                                                                         >  2:1179518/1‑150 (MQ=255)

                                                             aagTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATc                                                                                         <  2:3942964/150‑1 (MQ=255)

                                                              agTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGCTCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCg                                                                                        <  2:1070267/150‑1 (MQ=255)

                                                              agTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCg                                                                                        <  2:1674481/150‑1 (MQ=255)

                                                                   ggTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCcaa                                                                                   <  1:3559153/150‑1 (MQ=255)

                                                                     tgtAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCcaaca                                                                                 <  2:4248992/150‑1 (MQ=255)

                                                                     tgtAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCcaaca                                                                                 <  1:4169502/150‑1 (MQ=255)

                                                                        aagtaaTTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAAcc                                                                              >  2:4538438/1‑150 (MQ=255)

                                                                          gtaaTTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAAcccc                                                                            >  2:3354927/1‑150 (MQ=255)

                                                                           taaTTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCa                                                                           >  1:112385/1‑150 (MQ=255)

                                                                              ttGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGc                                                                        <  1:2425577/150‑1 (MQ=255)

                                                                               tgctgcATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCa                                                                       <  1:2698389/150‑1 (MQ=255)

                                                                                gctgcATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCaa                                                                      >  1:1558792/1‑150 (MQ=255)

                                                                                  tgcATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCNCGTTCGATCGGCCAACAACCCCATGCAAgg                                                                    <  1:4284173/150‑1 (MQ=255)

                                                                                  tgcATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAgg                                                                    >  2:4313076/1‑150 (MQ=255)

                                                                                  tgcATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAgg                                                                    >  2:1370091/1‑150 (MQ=255)

                                                                                   gcATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGa                                                                   <  1:2471051/150‑1 (MQ=255)

                                                                                   gcATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGa                                                                   <  1:3694771/150‑1 (MQ=255)

                                                                                    cATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGAt                                                                  >  2:2574962/1‑150 (MQ=255)

                                                                                    cATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGAt                                                                  >  2:3365712/1‑150 (MQ=255)

                                                                                     aTAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATc                                                                 >  2:828769/1‑150 (MQ=255)

                                                                                       aGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCgg                                                               >  1:2310987/1‑150 (MQ=255)

                                                                                        gATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGa                                                              >  2:2507957/1‑150 (MQ=255)

                                                                                          tCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGaaa                                                            <  1:861185/150‑1 (MQ=255)

                                                                                           cATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAAt                                                           >  2:2899081/1‑150 (MQ=255)

                                                                                             ttttGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAAtag                                                         <  1:437862/150‑3 (MQ=255)

                                                                                              tttGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGaaaaagc                                                        >  1:4338485/1‑146 (MQ=255)

                                                                                                tGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCAt                                                      >  1:826124/1‑150 (MQ=255)

                                                                                                tGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCAt                                                      >  2:4629999/1‑150 (MQ=255)

                                                                                                tGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCAt                                                      >  2:3454501/1‑150 (MQ=255)

                                                                                                 gCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATc                                                     >  2:1656194/1‑150 (MQ=255)

                                                                                                  ccGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATcc                                                    >  1:886445/1‑150 (MQ=255)

                                                                                                  ccGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATcc                                                    >  1:3414106/1‑150 (MQ=255)

                                                                                                   cGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCa                                                   >  1:2908483/1‑150 (MQ=255)

                                                                                                   cGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCa                                                   >  2:651560/1‑150 (MQ=255)

                                                                                                    gTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAg                                                  <  1:473966/150‑1 (MQ=255)

                                                                                                     ttCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGt                                                 >  1:3775749/1‑150 (MQ=255)

                                                                                                       cATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGc                                               >  2:1884140/1‑150 (MQ=255)

                                                                                                       cATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGc                                               >  2:3129371/1‑150 (MQ=255)

                                                                                                       cATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGc                                               >  2:3779245/1‑150 (MQ=255)

                                                                                                        aTACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCa                                              <  1:1431707/150‑1 (MQ=255)

                                                                                                         tACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCAc                                             >  2:391188/1‑150 (MQ=255)

                                                                                                          aCTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACg                                            >  2:3271023/1‑150 (MQ=255)

                                                                                                              agaTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCg                                        <  1:1786895/150‑1 (MQ=255)

                                                                                                                   tGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCaa                                   <  2:2013024/150‑1 (MQ=255)

                                                                                                                   tGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCaa                                   <  1:3622173/150‑1 (MQ=255)

                                                                                                                      cGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCaaaaa                                >  2:3169160/1‑150 (MQ=255)

                                                                                                                      cGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCaaaaa                                <  1:4222914/150‑1 (MQ=255)

                                                                                                                       gTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCCAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAAc                               >  2:3514227/1‑150 (MQ=255)

                                                                                                                       gTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAAc                               >  1:1847794/1‑150 (MQ=255)

                                                                                                                        tGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACa                              >  1:187966/1‑150 (MQ=255)

                                                                                                                          cTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATg                            >  1:645064/1‑150 (MQ=255)

                                                                                                                          cTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATg                            >  1:2117394/1‑150 (MQ=255)

                                                                                                                          cTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATg                            >  1:376232/1‑150 (MQ=255)

                                                                                                                            ataGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGt                          <  2:2737680/150‑1 (MQ=255)

                                                                                                                            ataGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGt                          <  2:838930/150‑1 (MQ=255)

                                                                                                                               ggCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTcc                       >  2:3107141/1‑150 (MQ=255)

                                                                                                                                gCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCg                      >  1:2810900/1‑150 (MQ=255)

                                                                                                                                 ccAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGa                     >  2:4226249/1‑150 (MQ=255)

                                                                                                                                  cAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGAc                    <  1:1018277/150‑1 (MQ=255)

                                                                                                                                   aGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACa                   >  1:2737341/1‑150 (MQ=255)

                                                                                                                                     tACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACaaa                 >  1:1297865/1‑150 (MQ=255)

                                                                                                                                        acaGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAAcc              <  2:2391148/150‑1 (MQ=255)

                                                                                                                                             cGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAg         >  2:3137374/1‑150 (MQ=255)

                                                                                                                                              gATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAgg        >  2:148880/1‑150 (MQ=255)

                                                                                                                                                  ccGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATg    <  1:1225057/150‑1 (MQ=255)

                                                                                                                                                  ccGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATg    <  1:1651412/150‑1 (MQ=255)

                                                                                                                                                  ccGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATg    >  1:1964270/1‑150 (MQ=255)

CATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCATAGATCATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCC  >  CP009273/3828872‑3829169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`