Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,829,113 C→A 38.2% A59A (GCG→GCT)  yicJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,829,1130CA38.2% 432.8 / 306.7 295A59A (GCG→GCT) yicJputative transporter
Reads supporting (aligned to +/- strand):  ref base C (94/88);  new base A (64/49);  total (158/137)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

ATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTC  >  CP009273/3828964‑3829260
                                                                                                                                                     |                                                                                                                                                   
aTTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATc                                                                                                                                                     <  2:3179247/150‑1 (MQ=255)
  tttGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGc                                                                                                                                                   >  1:4338533/1‑150 (MQ=255)
    tGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCAt                                                                                                                                                 >  2:678362/1‑150 (MQ=255)
    tGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCAt                                                                                                                                                 >  2:4630169/1‑150 (MQ=255)
     gCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCACGGATCGGAAATAGCATc                                                                                                                                                >  2:1656785/1‑150 (MQ=255)
      ccGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATcc                                                                                                                                               >  1:886445/1‑150 (MQ=255)
      ccGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATcc                                                                                                                                               >  1:3414106/1‑150 (MQ=255)
       cGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCa                                                                                                                                              >  1:2908483/1‑150 (MQ=255)
       cGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCa                                                                                                                                              <  1:1439987/150‑1 (MQ=255)
        gTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAg                                                                                                                                             <  1:474035/150‑1 (MQ=255)
           cATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGc                                                                                                                                          >  2:3473492/1‑150 (MQ=255)
            aTACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCa                                                                                                                                         >  2:1909570/1‑150 (MQ=255)
            aTACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCa                                                                                                                                         >  2:4346836/1‑150 (MQ=255)
             tACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCAc                                                                                                                                        >  1:282580/1‑150 (MQ=255)
             tACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCAc                                                                                                                                        >  2:391188/1‑150 (MQ=255)
              aCTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACg                                                                                                                                       >  2:3271023/1‑150 (MQ=255)
                   gaTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGa                                                                                                                                  >  1:2382103/1‑150 (MQ=255)
                    aTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCAAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGAGCACGAGCGAc                                                                                                                                 >  1:814549/1‑150 (MQ=255)
                       tGGCGTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCaa                                                                                                                              <  2:2013024/150‑1 (MQ=255)
                       tGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCaa                                                                                                                              <  1:3622173/150‑1 (MQ=255)
                       tGGCGTGCTATAGGCCAGTACACAGAAGATCCCTAATGGCAGTGCGCCAAACAGGACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCaa                                                                                                                              <  2:4078946/150‑1 (MQ=255)
                           gTGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCCAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAAc                                                                                                                          >  2:3514227/1‑150 (MQ=255)
                           gTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAAc                                                                                                                          >  1:1847794/1‑150 (MQ=255)
                            tGCTATAGGCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACa                                                                                                                         >  1:187974/1‑150 (MQ=255)
                             gCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACAt                                                                                                                        <  1:1815470/150‑1 (MQ=255)
                                   ggCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTcc                                                                                                                  <  1:2920108/150‑1 (MQ=255)
                                   ggCCAGTACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTcc                                                                                                                  >  2:3107141/1‑150 (MQ=255)
                                     ccAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGa                                                                                                                >  2:4219118/1‑150 (MQ=255)
                                         tACACAGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACaaa                                                                                                            >  1:1297865/1‑150 (MQ=255)
                                            acaGACGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAAcc                                                                                                         <  1:3133982/150‑1 (MQ=255)
                                            acaGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAAcc                                                                                                         >  2:2324510/1‑150 (MQ=255)
                                              aGACGATCCCTCATGGCAGTGCGCCAAACAGTTCCCTCGGACGTAATTTACACCAGCGAGAGCTCGTTCGATCGGCCAACAACCCCATGCAAGGTTCGGAAATAGCATCCTTTGCATGAGCGACGAAATCCATGGTTCAGAAAAAACCgg                                                                                                       <  2:1366300/150‑1 (MQ=255)
                                                 cGATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAg                                                                                                    >  2:3137374/1‑150 (MQ=255)
                                                  gATCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAgg                                                                                                   >  2:148880/1‑150 (MQ=255)
                                                   aTCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGa                                                                                                  >  2:289952/1‑150 (MQ=255)
                                                   aTCCCTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGa                                                                                                  >  2:2760834/1‑150 (MQ=255)
                                                     cccTAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAAt                                                                                                <  1:2737139/150‑1 (MQ=255)
                                                     cccGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAAt                                                                                                <  1:2197624/150‑1 (MQ=255)
                                                     cccGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAAt                                                                                                <  1:827229/150‑1 (MQ=255)
                                                      cctaaTGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATg                                                                                               >  1:749725/4‑150 (MQ=255)
                                                      cctaaTGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATg                                                                                               >  1:1514631/4‑150 (MQ=255)
                                                      cctaaTGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATg                                                                                               <  1:253462/147‑1 (MQ=255)
                                                      ccGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATg                                                                                               <  1:1225057/150‑1 (MQ=255)
                                                       cGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGc                                                                                              >  2:766540/1‑150 (MQ=255)
                                                         aaTGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCa                                                                                            <  1:2120920/150‑1 (MQ=255)
                                                                gTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGAGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAatat                                                                                     <  1:3848997/150‑1 (MQ=255)
                                                                 tGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATc                                                                                    <  1:3114329/150‑1 (MQ=255)
                                                                  gcgcCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCg                                                                                   <  1:416340/150‑1 (MQ=255)
                                                                  gcgcCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCg                                                                                   <  2:2823700/150‑1 (MQ=255)
                                                                    gcCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAACCATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGt                                                                                 <  2:1306929/150‑1 (MQ=255)
                                                                    gcCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGt                                                                                 <  1:1680869/150‑1 (MQ=255)
                                                                     ccAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGta                                                                                <  1:781425/150‑1 (MQ=255)
                                                                          cAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATaaaa                                                                           >  1:225578/1‑150 (MQ=255)
                                                                          cAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATaaaa                                                                           >  2:1720210/1‑150 (MQ=255)
                                                                           aGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAg                                                                          <  2:1588399/150‑1 (MQ=255)
                                                                                ccACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAAcat                                                                     >  1:1009967/1‑150 (MQ=255)
                                                                                ccACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAAcat                                                                     <  1:1421405/150‑1 (MQ=255)
                                                                                    ggACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCata                                                                 <  2:3576816/150‑1 (MQ=255)
                                                                                     gACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCatat                                                                <  2:1885823/150‑1 (MQ=255)
                                                                                      aCGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCatata                                                               >  2:1519494/1‑150 (MQ=255)
                                                                                        gAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCAtatata                                                             >  2:4421987/1‑150 (MQ=255)
                                                                                           aTTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACa                                                          >  1:4052543/1‑150 (MQ=255)
                                                                                           aTTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACa                                                          >  1:1774536/1‑150 (MQ=255)
                                                                                            tttACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACAt                                                         <  1:669337/150‑1 (MQ=255)
                                                                                              tACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTa                                                       <  2:2603162/150‑1 (MQ=255)
                                                                                              tACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTa                                                       >  1:413121/1‑150 (MQ=255)
                                                                                                     gCGAGAGCTCGTTCGATCGGCCAACAACCACATGCTAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGGTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATc                                                <  2:287199/150‑1 (MQ=255)
                                                                                                      cGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCg                                               <  2:648100/150‑1 (MQ=255)
                                                                                                       gagaGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGa                                              >  2:38011/1‑150 (MQ=255)
                                                                                                        agagCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGaa                                             <  2:3772146/150‑1 (MQ=255)
                                                                                                        agagCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGaa                                             <  2:1724083/150‑1 (MQ=255)
                                                                                                          agCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGaaaa                                           >  1:1491697/1‑150 (MQ=255)
                                                                                                             gcgTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAaataa                                        <  1:106451/150‑1 (MQ=255)
                                                                                                             gcgTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAaataa                                        <  1:3054955/150‑1 (MQ=255)
                                                                                                              cgTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAaataat                                       <  1:4228909/150‑1 (MQ=255)
                                                                                                               gTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAAtg                                      <  2:3154623/150‑1 (MQ=255)
                                                                                                                   gATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggc                                  >  1:388976/1‑150 (MQ=255)
                                                                                                                   gATCGGCCAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggc                                  >  1:3698551/1‑150 (MQ=255)
                                                                                                                    aTCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggct                                 >  1:1040013/1‑150 (MQ=255)
                                                                                                                     tCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggctg                                <  2:1090365/150‑1 (MQ=255)
                                                                                                                         ccAACAACCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcgg                            >  1:998338/1‑150 (MQ=255)
                                                                                                                          caacaaCCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcggc                           <  2:187966/150‑1 (MQ=255)
                                                                                                                           aacaacCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGgcggcg                          <  2:3413224/150‑1 (MQ=255)
                                                                                                                            acaacCCCATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGt                         <  1:4538448/150‑1 (MQ=255)
                                                                                                                             caacCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGtc                        <  2:3697456/150‑1 (MQ=255)
                                                                                                                                ccccATGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTcc                     >  1:4404983/1‑150 (MQ=255)
                                                                                                                                 cccATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTccc                    >  2:2815866/1‑150 (MQ=255)
                                                                                                                                  ccATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa                   >  1:3155124/1‑150 (MQ=255)
                                                                                                                                    aTGCAAGGATCGGAAATCGCATCTAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATg                 <  2:4257375/150‑1 (MQ=255)
                                                                                                                                     tGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGc                >  2:3209296/1‑150 (MQ=255)
                                                                                                                                     tGCAAGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGc                <  1:2645968/150‑1 (MQ=255)
                                                                                                                                      gCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGcc               >  2:2446735/1‑150 (MQ=255)
                                                                                                                                       cAAGGATCGGAAATCGCATCCAGTGCACGATAGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCa              <  1:3360223/150‑1 (MQ=255)
                                                                                                                                         aGGATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATa            >  2:1136889/1‑150 (MQ=255)
                                                                                                                                          ggATCGGAAATAGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAGGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATaa           <  1:1179518/150‑1 (MQ=255)
                                                                                                                                           gATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAAc          <  1:1370091/150‑1 (MQ=255)
                                                                                                                                            aTCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAAcc         >  1:533492/1‑150 (MQ=255)
                                                                                                                                              cGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCaa       >  1:2028528/1‑150 (MQ=255)
                                                                                                                                                   aTCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTtc  <  1:3454501/150‑1 (MQ=255)
                                                                                                                                                   aTCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTtc  <  1:4629999/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATTTTGCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAGTGCACGAGCGACCAAAAACATGGTTCCGACAAAACCGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTC  >  CP009273/3828964‑3829260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑