Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,851,696 G→T 5.2% S23R (AGC→AGA)  yidJ ← sulfatase/phosphatase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,851,6960GT 5.2% 1040.1 / 19.5 268S23R (AGC→AGA) yidJsulfatase/phosphatase superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (122/132);  new base T (7/7);  total (129/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 273 total aligned reads displayed.

TTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATC  >  CP009273/3851547‑3851845
                                                                                                                                                     |                                                                                                                                                     
ttgGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCg                                                                                                                                                       >  1:1579167/1‑150 (MQ=255)
  ggTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCt                                                                                                                                                     <  1:2086741/150‑1 (MQ=255)
  ggTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCt                                                                                                                                                     <  1:1737158/150‑1 (MQ=255)
    tCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCtat                                                                                                                                                   >  2:3401391/1‑150 (MQ=255)
         gggCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCaa                                                                                                                                              <  1:2535895/150‑1 (MQ=255)
         gggCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCaa                                                                                                                                              <  2:1983683/150‑1 (MQ=255)
           gCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAAcc                                                                                                                                            >  1:2826087/1‑150 (MQ=255)
            ccGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCg                                                                                                                                           <  2:3297647/150‑1 (MQ=255)
              ggACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGAc                                                                                                                                         <  2:556499/150‑1 (MQ=255)
                 cTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCat                                                                                                                                      >  2:1094765/1‑150 (MQ=255)
                  tGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCata                                                                                                                                     >  1:3530007/1‑150 (MQ=255)
                     ttAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATtg                                                                                                                                  >  1:2396115/1‑150 (MQ=255)
                      tAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATtgg                                                                                                                                 >  2:706941/1‑150 (MQ=255)
                          gTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCTCTATAGCAACCGACCATATTGGTGGc                                                                                                                             <  2:3372742/150‑1 (MQ=255)
                           tAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGcc                                                                                                                            >  1:3364997/1‑150 (MQ=255)
                            aGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCt                                                                                                                           >  2:262756/1‑150 (MQ=255)
                            aGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCt                                                                                                                           <  2:1582426/150‑1 (MQ=255)
                                 ccGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTa                                                                                                                      <  1:3488009/150‑1 (MQ=255)
                                  cGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTAt                                                                                                                     >  2:2413600/1‑150 (MQ=255)
                                      aaaTAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGt                                                                                                                 <  2:4092277/150‑1 (MQ=255)
                                       aaTAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTc                                                                                                                <  2:191112/150‑1 (MQ=255)
                                         tAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCAt                                                                                                              <  1:343170/150‑1 (MQ=255)
                                          aGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATg                                                                                                             >  2:4503102/1‑150 (MQ=255)
                                          aGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATg                                                                                                             >  1:190322/1‑150 (MQ=255)
                                          aGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGAATTCAGCGGTTTACCGCAATAGCAACCGACCATATTGGTGGCCTGGGTCTCGGTCATg                                                                                                             >  1:2686585/1‑150 (MQ=255)
                                            tCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGAc                                                                                                           >  2:4158177/1‑150 (MQ=255)
                                            tCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGAc                                                                                                           <  2:1760106/150‑1 (MQ=255)
                                              cGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGTCCTGGGTATCGGTCATGACGa                                                                                                         >  1:997688/1‑150 (MQ=255)
                                              cGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGa                                                                                                         <  1:593906/150‑1 (MQ=255)
                                              cGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGa                                                                                                         <  1:593654/150‑1 (MQ=255)
                                                gCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAAc                                                                                                       <  1:663699/150‑1 (MQ=255)
                                                 cccGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACa                                                                                                      <  2:2509963/150‑1 (MQ=255)
                                                  ccGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAg                                                                                                     >  1:1322755/1‑150 (MQ=255)
                                                  ccGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAg                                                                                                     <  1:1737300/150‑1 (MQ=255)
                                                      gCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGaaaa                                                                                                 >  2:23456/1‑150 (MQ=255)
                                                           cGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTggg                                                                                            >  2:4324949/1‑150 (MQ=255)
                                                            gTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCAGCAAGACTATCAATATTATGCGTATTCAGCGGATTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCCTGACGAACAGAAAATTgggg                                                                                           >  1:1182402/1‑150 (MQ=255)
                                                             tACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGc                                                                                          <  1:1231102/150‑1 (MQ=255)
                                                                aaaCCGGTGAACAGGTGTAGGCGCAATTAAAGCGAATAACTTCCGCCTCAAGACTATCAATATTTAGCGTATACAGCGGTTTACAGCTATAGCAACCGACCATATTGGTGGCATGGGGATCGGTCATGACGTACAAAAAATTGGGTCGtt                                                                                       >  2:4600987/1‑150 (MQ=255)
                                                                 aaCCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGttt                                                                                      >  2:557985/1‑150 (MQ=255)
                                                                  aCCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTc                                                                                     >  2:3088465/1‑150 (MQ=255)
                                                                  aCCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTc                                                                                     <  1:1087852/150‑1 (MQ=255)
                                                                  aCCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTc                                                                                     <  2:401295/150‑1 (MQ=255)
                                                                    cGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCAt                                                                                   <  1:4355174/150‑1 (MQ=255)
                                                                     ggTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCTCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATa                                                                                  <  1:840576/150‑1 (MQ=255)
                                                                     ggTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATa                                                                                  >  1:476286/1‑150 (MQ=255)
                                                                      gTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAg                                                                                 <  1:3051876/150‑1 (MQ=255)
                                                                      gTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAg                                                                                 <  2:2846659/150‑1 (MQ=255)
                                                                       tGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAgg                                                                                >  2:286530/1‑150 (MQ=255)
                                                                        gAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGc                                                                               >  1:2576925/1‑150 (MQ=255)
                                                                           cAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCTCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAACTTGGGGCGTTTCATAGGCTAt                                                                            <  2:925178/150‑1 (MQ=255)
                                                                               tgtAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCtt                                                                        <  1:957916/150‑1 (MQ=255)
                                                                                 tAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTcc                                                                      >  2:2690659/1‑150 (MQ=255)
                                                                                 tAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTcc                                                                      <  2:2014156/150‑1 (MQ=255)
                                                                                   ggCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCg                                                                    >  1:2280058/1‑150 (MQ=255)
                                                                                        aaTTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCggg                                                               <  2:1327194/150‑1 (MQ=255)
                                                                                          ttAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAg                                                             >  1:4161840/1‑150 (MQ=255)
                                                                                           tAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTATACAGCTATAGCACCCGACCATAATGGTGGACTGGGTATCGGTCAAGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGt                                                            >  1:1039385/1‑150 (MQ=255)
                                                                                            aaaGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTa                                                           >  2:4246691/1‑150 (MQ=255)
                                                                                             aaGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTaa                                                          <  2:4339685/150‑1 (MQ=255)
                                                                                              aGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGTAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTaaa                                                         <  1:4509182/150‑1 (MQ=255)
                                                                                                 gAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGt                                                      >  1:2145589/1‑150 (MQ=255)
                                                                                                    tACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTAc                                                   <  1:515061/150‑1 (MQ=255)
                                                                                                     aCCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACg                                                  <  1:401250/150‑1 (MQ=255)
                                                                                                      ccTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACgg                                                 >  2:815824/1‑150 (MQ=255)
                                                                                                       cTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGc                                                >  1:2354645/1‑150 (MQ=255)
                                                                                                        ttCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCTCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCt                                               >  1:3827726/1‑150 (MQ=255)
                                                                                                         tCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTg                                              <  2:2100009/150‑1 (MQ=255)
                                                                                                             ccgccAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTc                                          >  1:1498318/1‑150 (MQ=255)
                                                                                                                ccAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAATGGTTACGGCTGGGTCGTg                                       >  2:3977532/1‑150 (MQ=255)
                                                                                                                ccAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTg                                       <  1:740402/150‑1 (MQ=255)
                                                                                                                ccAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTg                                       <  1:4116228/150‑1 (MQ=255)
                                                                                                                 cAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGa                                      >  1:979777/1‑150 (MQ=255)
                                                                                                                  aGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGAc                                     <  1:2027586/150‑1 (MQ=255)
                                                                                                                  aGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGAc                                     >  2:1852766/1‑150 (MQ=255)
                                                                                                                   gACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACg                                    <  2:2978870/150‑1 (MQ=255)
                                                                                                                    aCTATGAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGa                                   <  2:4618297/150‑1 (MQ=255)
                                                                                                                     cTATCAATATTTTGCGTATTCAGCGGTTTACCTCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGAt                                  >  1:2256329/1‑150 (MQ=255)
                                                                                                                     cTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGAt                                  >  1:4406729/1‑150 (MQ=255)
                                                                                                                     cTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCGTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGAt                                  <  1:1822121/150‑1 (MQ=255)
                                                                                                                      tATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATg                                 >  1:3930244/1‑150 (MQ=255)
                                                                                                                         cAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATgccg                              <  1:4259167/150‑1 (MQ=255)
                                                                                                                         cAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATgccg                              >  2:1419729/1‑150 (MQ=255)
                                                                                                                          aaTATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATgccgc                             >  1:4040617/1‑150 (MQ=255)
                                                                                                                            tatTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTTGTGGCCTGGGTATCGGTAATGACGAACAGAAAATTGTGTCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATgccgcaa                           >  2:1702998/1‑148 (MQ=255)
                                                                                                                             atTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAg                          >  2:3879710/1‑150 (MQ=255)
                                                                                                                                   cGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCa                    >  1:2469963/1‑150 (MQ=255)
                                                                                                                                    gTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCaa                   >  1:3247581/1‑150 (MQ=255)
                                                                                                                                     tATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCaaa                  >  1:3200875/1‑150 (MQ=255)
                                                                                                                                        tCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAAt               >  2:3544193/1‑150 (MQ=255)
                                                                                                                                        tCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAAt               >  2:1799763/1‑150 (MQ=255)
                                                                                                                                          aGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa             >  2:1250382/1‑150 (MQ=255)
                                                                                                                                          aGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa             <  2:37318/150‑1 (MQ=255)
                                                                                                                                          aGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa             <  2:2162046/150‑1 (MQ=255)
                                                                                                                                             ggTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGt          <  1:3719880/150‑1 (MQ=255)
                                                                                                                                             ggTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGt          >  2:2603142/1‑150 (MQ=255)
                                                                                                                                             ggTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGt          >  2:793063/1‑150 (MQ=255)
                                                                                                                                                 tACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGAc      <  2:384340/150‑1 (MQ=255)
                                                                                                                                                 tACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGAc      <  1:3621636/150‑1 (MQ=255)
                                                                                                                                                   ccGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACaa    <  2:2356212/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGCAGGCGTACAAACCGGTGAACAGGTGTAGGCGGAATTAAAGCGAATACCTTCCGCCGCCAGACTATCAATATTTTGCGTATTCAGCGGTTTACCGCTATAGCAACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATC  >  CP009273/3851547‑3851845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑