BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,653	C→A	91.6%	E44* (GAA→TAA)	ibpA ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,653	0	C	A	91.6%	812.8 / 69.4	285	E44* (GAA→TAA)	ibpA	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base C (16/8); new base A (133/128); total (149/136)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 285 total aligned reads displayed.

GTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAA  >  CP009273/3860506‑3860799

gTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGt                                                                                                                                                  >  2:3274682/1‑150 (MQ=255)

    aGCGATGCCCTGGTACAGATAGGTGCGCTCTTCTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTAc                                                                                                                                              >  1:4190994/1‑150 (MQ=255)

        aggCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGt                                                                                                                                          <  2:1835229/148‑1 (MQ=255)

         tGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGtt                                                                                                                                         >  1:291810/1‑150 (MQ=255)

           cccTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCATATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCa                                                                                                                                       <  1:3478056/150‑1 (MQ=255)

             cTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAAc                                                                                                                                     <  2:2031143/150‑1 (MQ=255)

             cTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAAc                                                                                                                                     >  2:702901/1‑150 (MQ=255)

              tGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACg                                                                                                                                    >  1:4528005/1‑150 (MQ=255)

              tGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACg                                                                                                                                    >  1:1205060/1‑150 (MQ=255)

              tGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACAACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAAACAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACg                                                                                                                                    >  2:435545/1‑150 (MQ=255)

               ggTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGt                                                                                                                                   >  2:2159745/1‑150 (MQ=255)

                 tACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTta                                                                                                                                 <  1:312910/150‑1 (MQ=255)

                  aCAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTtat                                                                                                                                <  1:1325164/150‑1 (MQ=255)

                  aCAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTtat                                                                                                                                <  1:3572288/150‑1 (MQ=255)

                      aTAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACgg                                                                                                                            <  1:2465441/150‑1 (MQ=255)

                       tAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGa                                                                                                                           >  2:2845227/1‑150 (MQ=255)

                       tAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGa                                                                                                                           <  2:865833/150‑1 (MQ=255)

                        aGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAg                                                                                                                          <  1:3190590/150‑1 (MQ=255)

                           tGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGt                                                                                                                       <  2:1419907/150‑1 (MQ=255)

                           tGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGt                                                                                                                       <  1:3357833/150‑1 (MQ=255)

                               ctctTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAgcc                                                                                                                   <  2:2530805/150‑1 (MQ=255)

                               ctctTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAgcc                                                                                                                   <  1:1400900/150‑1 (MQ=255)

                                 ctTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAgccgc                                                                                                                 >  1:629070/1‑150 (MQ=255)

                                   ttttGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCa                                                                                                               >  1:2718347/1‑150 (MQ=255)

                                    tttGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCAt                                                                                                              <  2:2318888/150‑1 (MQ=255)

                                     ttGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCAtt                                                                                                             <  2:3777216/150‑1 (MQ=255)

                                      tGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTa                                                                                                            >  2:332365/1‑150 (MQ=255)

                                       gTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTAc                                                                                                           >  1:3497734/1‑150 (MQ=255)

                                        ttCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTAct                                                                                                          >  2:3489174/1‑150 (MQ=255)

                                         tcgtcgGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActc                                                                                                         >  1:1632795/1‑150 (MQ=255)

                                         tcgtcgGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActc                                                                                                         >  1:1632612/1‑150 (MQ=255)

                                              ggCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggct                                                                                                    <  1:813902/150‑1 (MQ=255)

                                              ggCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggct                                                                                                    >  1:278212/1‑150 (MQ=255)

                                               gCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctc                                                                                                   >  1:2252029/1‑150 (MQ=255)

                                                 gTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctctg                                                                                                 >  2:575131/1‑150 (MQ=255)

                                                 gTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctctg                                                                                                 <  2:1283765/150‑1 (MQ=255)

                                                  tGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctctgg                                                                                                <  2:1683565/150‑1 (MQ=255)

                                                    aGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgtt                                                                                              <  2:4576235/150‑1 (MQ=255)

                                                    aGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTCTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgtt                                                                                              <  1:2499317/150‑1 (MQ=255)

                                                      cACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgttgt                                                                                            <  2:3382028/150‑1 (MQ=255)

                                                       aCCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgttgtt                                                                                           <  2:3616113/150‑1 (MQ=255)

                                                          tttCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTc                                                                                        <  1:872997/150‑1 (MQ=255)

                                                             caccacCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAAAGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTaa                                                                                     <  1:4329629/150‑1 (MQ=255)

                                                              accaccAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAg                                                                                    >  1:959178/1‑150 (MQ=255)

                                                                 accaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTgg                                                                                 <  2:3544415/150‑1 (MQ=255)

                                                                 accaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTgg                                                                                 <  2:4101557/150‑1 (MQ=255)

                                                                      cagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaa                                                                            >  2:2613717/1‑150 (MQ=255)

                                                                       agATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaac                                                                           >  1:3983539/1‑150 (MQ=255)

                                                                       agATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaac                                                                           >  2:62850/1‑150 (MQ=255)

                                                                        gATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaaca                                                                          <  2:822034/150‑1 (MQ=255)

                                                                         aTTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaacaa                                                                         >  1:210742/1‑150 (MQ=255)

                                                                         aTTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaacaa                                                                         >  2:4196736/1‑150 (MQ=255)

                                                                           tATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaacaaac                                                                       >  2:2644428/1‑150 (MQ=255)

                                                                           tATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaacaaac                                                                       >  1:1064432/1‑150 (MQ=255)

                                                                             tCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACgg                                                                     <  1:3003311/150‑1 (MQ=255)

                                                                               cTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTc                                                                   >  2:1082279/1‑150 (MQ=255)

                                                                                tGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCa                                                                  <  1:503904/150‑1 (MQ=255)

                                                                                 gggCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCaa                                                                 <  2:1939981/150‑1 (MQ=255)

                                                                                     ggTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATcc                                                             >  2:1619560/1‑150 (MQ=255)

                                                                                      gTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCa                                                            <  2:4215610/150‑1 (MQ=255)

                                                                                      gTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCa                                                            <  1:696278/150‑1 (MQ=255)

                                                                                          tttCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAg                                                        <  1:4071165/150‑1 (MQ=255)

                                                                                            tCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCa                                                      <  2:3172883/150‑1 (MQ=255)

                                                                                            tCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCa                                                      <  1:3166623/150‑1 (MQ=255)

                                                                                             ccAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAg                                                     >  2:580977/1‑150 (MQ=255)

                                                                                                gTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAAc                                                  <  2:1497052/150‑1 (MQ=255)

                                                                                                gTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAAc                                                  <  1:560854/150‑1 (MQ=255)

                                                                                                 ttCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACg                                                 <  2:2779467/150‑1 (MQ=255)

                                                                                                 ttCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACg                                                 <  2:1203056/150‑1 (MQ=255)

                                                                                                     ctctCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTaa                                             >  2:2637822/1‑150 (MQ=255)

                                                                                                     ctctCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTaa                                             >  2:3069368/1‑150 (MQ=255)

                                                                                                      tctcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTaaa                                            <  1:1562392/150‑1 (MQ=255)

                                                                                                          aGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCgg                                        <  1:3652915/150‑1 (MQ=255)

                                                                                                           gCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCggg                                       <  2:4520855/150‑1 (MQ=255)

                                                                                                             aaaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGa                                     <  1:749813/150‑1 (MQ=255)

                                                                                                             aaaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGa                                     >  2:4280607/1‑150 (MQ=255)

                                                                                                              aaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGAt                                    <  1:2521520/150‑1 (MQ=255)

                                                                                                                  cAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAAt                                <  1:4561756/150‑1 (MQ=255)

                                                                                                                   aGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATc                               >  1:3916337/1‑150 (MQ=255)

                                                                                                                   aGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATc                               <  2:2196630/150‑1 (MQ=255)

                                                                                                                     ccACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCaa                             <  2:291810/150‑1 (MQ=255)

                                                                                                                        caGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGt                          <  1:56874/150‑1 (MQ=255)

                                                                                                                           cGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTAc                       >  1:1957332/1‑150 (MQ=255)

                                                                                                                            gATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACg                      >  2:1183468/1‑150 (MQ=255)

                                                                                                                            gATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATACAATAGCAGAACGGAAAAGCGGGGATAAATCAAAGTTACg                      >  1:4081095/1‑150 (MQ=255)

                                                                                                                             aTAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGc                     <  2:1875573/150‑1 (MQ=255)

                                                                                                                              tAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCa                    >  2:2176077/1‑150 (MQ=255)

                                                                                                                               aGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCAt                   >  2:3667697/1‑150 (MQ=255)

                                                                                                                               aGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCAt                   >  2:4033005/1‑150 (MQ=255)

                                                                                                                                gCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATa                  <  2:421978/150‑1 (MQ=255)

                                                                                                                                 cAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATaa                 <  1:1796008/150‑1 (MQ=255)

                                                                                                                                   aTGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATc               <  1:2605601/150‑1 (MQ=255)

                                                                                                                                   aTGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATc               >  1:2587361/1‑150 (MQ=255)

                                                                                                                                   aTGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATc               >  2:2015280/1‑150 (MQ=255)

                                                                                                                                    tGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCa              <  1:3842804/150‑1 (MQ=255)

                                                                                                                                           aTGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTc       <  1:4231485/150‑1 (MQ=255)

                                                                                                                                            tGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTcc      >  2:659608/1‑150 (MQ=255)

                                                                                                                                               ttttCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGa   <  1:1307405/150‑1 (MQ=255)

                                                                                                                                               ttttAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGa   >  2:3893745/1‑150 (MQ=255)

                                                                                                                                                tttAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGaa  <  1:2005216/150‑1 (MQ=255)

GTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAA  >  CP009273/3860506‑3860799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`