BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,874,397	C→A	13.8%	R62L (CGC→CTC)	recF ←	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,874,397	0	C	A	13.8%	1033.4 / 94.2	303	R62L (CGC→CTC)	recF	gap repair protein
Reads supporting (aligned to +/- strand): ref base C (132/129); new base A (18/24); total (150/153)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 303 total aligned reads displayed.

CAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTT  >  CP009273/3874249‑3874545

cAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCg                                                                                                                                                     <  1:1045322/150‑1 (MQ=255)

   gTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCGGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAAt                                                                                                                                                  <  1:824858/150‑1 (MQ=255)

       gcCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGAcg                                                                                                                                              >  1:2545985/1‑150 (MQ=255)

       gcCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGAcg                                                                                                                                              >  2:135147/1‑150 (MQ=255)

         cAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGAcgcg                                                                                                                                            <  2:1984859/150‑1 (MQ=255)

         cAGTTCCGCGACCTTAGGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGAcgcg                                                                                                                                            <  2:3566734/150‑1 (MQ=255)

          aGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAATCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGa                                                                                                                                           >  2:2114709/1‑150 (MQ=255)

          aGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGa                                                                                                                                           <  1:4638065/150‑1 (MQ=255)

          aGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGa                                                                                                                                           <  1:3435893/150‑1 (MQ=255)

             tCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCa                                                                                                                                        <  1:3146014/150‑1 (MQ=255)

                 cgACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCt                                                                                                                                    >  2:4020514/1‑150 (MQ=255)

                  gACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTg                                                                                                                                   >  1:1158771/1‑150 (MQ=255)

                  gACCTAATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGAGGAGAACAAACGCCTCCTGCTCAGGTCGAATGACGCGACCAATCTg                                                                                                                                   >  2:230966/1‑150 (MQ=255)

                   aCCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGc                                                                                                                                  >  2:630148/1‑150 (MQ=255)

                        aTGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACt                                                                                                                             <  1:1643038/150‑1 (MQ=255)

                        aTGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACt                                                                                                                             <  1:2806390/150‑1 (MQ=255)

                        aTGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACt                                                                                                                             >  1:2749914/1‑150 (MQ=255)

                              gTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCTAATGACGCGACCAATCTGCAAACTGCGaaa                                                                                                                       >  2:489750/1‑150 (MQ=255)

                               tCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAAc                                                                                                                      >  1:1278130/1‑150 (MQ=255)

                                  gTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGcc                                                                                                                   <  1:869609/150‑1 (MQ=255)

                                               ggACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGa                                                                                                      <  1:2662524/150‑1 (MQ=255)

                                                gACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAg                                                                                                     <  2:2391265/150‑1 (MQ=255)

                                                 aCTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGc                                                                                                    <  2:2743729/150‑1 (MQ=255)

                                                  cTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCg                                                                                                   <  2:2831146/150‑1 (MQ=255)

                                                  cTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCg                                                                                                   <  1:409880/150‑1 (MQ=255)

                                                    ttGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGta                                                                                                 <  1:2414856/150‑1 (MQ=255)

                                                    ttGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGta                                                                                                 <  1:943265/150‑1 (MQ=255)

                                                     tGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGtat                                                                                                >  2:3270700/1‑150 (MQ=255)

                                                        tGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGa                                                                                             >  1:1369219/1‑150 (MQ=255)

                                                        tGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATAGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCTACCAATCTGCAAACTGCGAAACGCCCGACAATGGCCGAGCGTATAGa                                                                                             >  1:4539527/1‑150 (MQ=255)

                                                         gTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGGATAGAt                                                                                            >  1:110039/1‑150 (MQ=255)

                                                           cGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATgg                                                                                          >  2:1229089/1‑150 (MQ=255)

                                                           cGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATgg                                                                                          <  1:643342/150‑1 (MQ=255)

                                                           cGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATgg                                                                                          >  1:1967652/1‑150 (MQ=255)

                                                               cTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTc                                                                                      <  2:3972158/150‑1 (MQ=255)

                                                                 gTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCa                                                                                    >  2:865906/1‑150 (MQ=255)

                                                                 gTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCa                                                                                    >  2:561526/1‑150 (MQ=255)

                                                                 gTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCa                                                                                    >  1:306569/1‑150 (MQ=255)

                                                                 gTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCa                                                                                    >  2:3301739/1‑150 (MQ=255)

                                                                  tttGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAg                                                                                   >  2:2762824/1‑150 (MQ=255)

                                                                    tGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCa                                                                                 <  2:2730939/150‑1 (MQ=255)

                                                                     gTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCAc                                                                                >  1:4443200/1‑150 (MQ=255)

                                                                       cTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGc                                                                              >  2:3073835/1‑150 (MQ=255)

                                                                         ttGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTg                                                                            <  1:4203554/150‑1 (MQ=255)

                                                                           ggTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGt                                                                          >  2:2558662/1‑150 (MQ=255)

                                                                            gTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGtt                                                                         >  1:484237/1‑150 (MQ=255)

                                                                            gTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGtt                                                                         >  1:3782896/1‑150 (MQ=255)

                                                                                    aaTCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACt                                                                 >  2:460195/1‑150 (MQ=255)

                                                                                    aaTCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACt                                                                 >  2:260961/1‑150 (MQ=255)

                                                                                    aaTCGCTGTCTAGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACt                                                                 >  1:3194735/1‑150 (MQ=255)

                                                                                     aTCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTg                                                                <  2:90258/150‑1 (MQ=255)

                                                                                      tCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGc                                                               <  2:3019265/150‑1 (MQ=255)

                                                                                         cTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGt                                                            <  1:3024156/150‑1 (MQ=255)

                                                                                          tGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGtt                                                           <  2:3907763/150‑1 (MQ=255)

                                                                                          tGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGtt                                                           >  2:1662518/1‑150 (MQ=255)

                                                                                          tGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGtt                                                           <  2:288232/150‑1 (MQ=255)

                                                                                          tGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGtt                                                           <  1:2429917/150‑1 (MQ=255)

                                                                                           gTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTg                                                          <  1:1383835/150‑1 (MQ=255)

                                                                                             ctcGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGc                                                        <  2:474063/150‑1 (MQ=255)

                                                                                               cgcgCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCAc                                                      >  1:1925462/1‑150 (MQ=255)

                                                                                                  gcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTa                                                   >  2:3839419/1‑150 (MQ=255)

                                                                                                  gcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTa                                                   <  2:3195555/150‑1 (MQ=255)

                                                                                                  gcTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTa                                                   <  1:4479385/150‑1 (MQ=255)

                                                                                                    tctTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTAcc                                                 >  2:1049205/1‑150 (MQ=255)

                                                                                                    tctTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTAcc                                                 >  1:3476913/1‑150 (MQ=255)

                                                                                                    tctTCGCCCTATAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTAcc                                                 >  1:1017889/1‑150 (MQ=255)

                                                                                                        cGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGaa                                             >  1:2057207/1‑150 (MQ=255)

                                                                                                         gCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGaaa                                            >  2:4485860/1‑150 (MQ=255)

                                                                                                           ccTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGt                                          >  1:3901721/1‑150 (MQ=255)

                                                                                                           ccTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGt                                          >  1:3901735/1‑150 (MQ=255)

                                                                                                               tAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTaaa                                      <  2:782460/150‑1 (MQ=255)

                                                                                                                aaTCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAg                                     <  1:4495783/150‑1 (MQ=255)

                                                                                                                 aTCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGc                                    <  2:36919/150‑1 (MQ=255)

                                                                                                                 aTCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGc                                    <  2:737182/150‑1 (MQ=255)

                                                                                                                  tCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGcc                                   <  1:135173/150‑1 (MQ=255)

                                                                                                                  tCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACCGCCGTTGGCACCTACCAGAAAGTTAAAGcc                                   <  2:4124254/150‑1 (MQ=255)

                                                                                                                    gCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCgg                                 >  1:3537342/1‑150 (MQ=255)

                                                                                                                    gCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCgg                                 >  2:1099653/1‑150 (MQ=255)

                                                                                                                     cccGTGGAGAACAAACGCCTCCTGCTCCTGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGATGGCACCTACCAGAAAGATAAAGCCggg                                >  2:4220142/1‑150 (MQ=255)

                                                                                                                     cccGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCggg                                <  2:561656/150‑1 (MQ=255)

                                                                                                                       cGTGGAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGgag                              <  2:1374149/150‑1 (MQ=255)

                                                                                                                        gTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGgaga                             >  2:2542552/1‑150 (MQ=255)

                                                                                                                         tGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGAt                            >  2:3605798/1‑150 (MQ=255)

                                                                                                                         tGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGAt                            <  2:1838621/150‑1 (MQ=255)

                                                                                                                          ggAGAACAAACGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATa                           <  1:2340588/150‑1 (MQ=255)

                                                                                                                             gaACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAgg                        <  1:247031/150‑1 (MQ=255)

                                                                                                                                 aaaCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgag                    <  1:1526459/150‑1 (MQ=255)

                                                                                                                                  aaCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgaga                   >  2:2652435/1‑150 (MQ=255)

                                                                                                                                  aaCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgaga                   >  2:1261633/1‑150 (MQ=255)

                                                                                                                                  aaCGCCTCCTGCTCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCgaga                   >  1:3707728/1‑150 (MQ=255)

                                                                                                                                   aCGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGAt                  <  1:2114709/150‑1 (MQ=255)

                                                                                                                                     gCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCTAGATcc                <  1:558248/150‑1 (MQ=255)

                                                                                                                                     gCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATcc                <  1:3143192/150‑1 (MQ=255)

                                                                                                                                        tcctGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCcgcg             >  2:3593592/1‑150 (MQ=255)

                                                                                                                                              tCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCa       <  1:43025/150‑1 (MQ=255)

                                                                                                                                              tCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCa       <  1:1226086/150‑1 (MQ=255)

                                                                                                                                              tCATGGAGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCa       <  2:2321016/150‑1 (MQ=255)

                                                                                                                                                 tGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATTGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATg    <  1:1903162/150‑1 (MQ=255)

                                                                                                                                                 tGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATg    <  2:3962510/150‑1 (MQ=255)

                                                                                                                                                 tGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATg    <  2:1395278/150‑1 (MQ=255)

CAGGTGCGCCAGTTCCGCGACCTTATGCCCGTCTGTACCGTCGATGCGGACTTTGCTGTCGCCCTGTTTGTCTTTGGTTAAGCCAATCGCTGTCTCGCGCTCTTCGCCCTGTAATCGCCCGTGGAGAACAAACGCCTCCTGCTCATGGCGAATGACGCGACCAATCTGCAAACTGCGAAACGCCCGACCATGGCCGAGCGTATAGATGGCTTCCAGCACGCTGGTTTTGCCACTGCCGTTGGCACCTACCAGAAAGTTAAAGCCGGGAGATAAGGCGAGATCCGCGGTTTCAATGTT  >  CP009273/3874249‑3874545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`