BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,891,421	C→A	15.3%	H14N (CAT→AAT)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,891,421	0	C	A	15.3%	812.4 / 85.8	248	H14N (CAT→AAT)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand): ref base C (115/95); new base A (19/19); total (134/114)
Fisher's exact test for biased strand distribution p-value = 6.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 258 total aligned reads displayed.

GAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGT  >  CP009273/3891272‑3891570

gAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATatc                                                                                                                                                       >  1:463263/1‑150 (MQ=255)

  aTCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATatcat                                                                                                                                                     >  2:1965235/1‑150 (MQ=255)

     aTTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATccc                                                                                                                                                  <  1:1912385/150‑1 (MQ=255)

          ccGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGcc                                                                                                                                             <  1:2922331/150‑1 (MQ=255)

          ccGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGcc                                                                                                                                             <  1:3459089/150‑1 (MQ=255)

              cTACCCTGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTg                                                                                                                                         <  2:2434701/150‑1 (MQ=255)

               tACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTgg                                                                                                                                        <  2:726467/150‑1 (MQ=255)

               tACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTgg                                                                                                                                        <  2:801249/150‑1 (MQ=255)

               tACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTgg                                                                                                                                        >  1:2303859/1‑150 (MQ=255)

                aCCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGa                                                                                                                                       >  1:4532020/1‑150 (MQ=255)

                 cccGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGaa                                                                                                                                      <  1:3270739/150‑1 (MQ=255)

                 cccGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGaa                                                                                                                                      <  1:4513970/150‑1 (MQ=255)

                  ccGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGaaa                                                                                                                                     <  2:3387681/150‑1 (MQ=255)

                    ggATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACa                                                                                                                                   <  2:1686008/150‑1 (MQ=255)

                    ggATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACa                                                                                                                                   >  1:3930446/1‑150 (MQ=255)

                    ggATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACa                                                                                                                                   >  1:2404809/1‑150 (MQ=255)

                    ggATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACa                                                                                                                                   <  1:3279438/150‑1 (MQ=255)

                     gATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAg                                                                                                                                  <  2:1799740/150‑1 (MQ=255)

                      aTTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAgg                                                                                                                                 <  2:4455179/150‑1 (MQ=255)

                      aTTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAgg                                                                                                                                 <  1:570077/150‑1 (MQ=255)

                        tGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGgcg                                                                                                                               >  2:640586/1‑150 (MQ=255)

                          gATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCa                                                                                                                             >  1:554883/1‑150 (MQ=255)

                          gATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCa                                                                                                                             >  1:1032209/1‑150 (MQ=255)

                                tCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGaa                                                                                                                       >  1:1822769/1‑150 (MQ=255)

                                  aTGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCAGTTGAACa                                                                                                                     >  1:3224488/1‑150 (MQ=255)

                                    gTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAgg                                                                                                                   <  2:1903593/150‑1 (MQ=255)

                                    gTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAgg                                                                                                                   >  1:728265/1‑150 (MQ=255)

                                        tGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATaa                                                                                                               >  1:2651744/1‑150 (MQ=255)

                                          aaaCAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATaaaa                                                                                                             <  2:3967968/150‑1 (MQ=255)

                                             cAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCg                                                                                                          >  1:2614137/1‑150 (MQ=255)

                                             cAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCg                                                                                                          <  2:1717896/150‑1 (MQ=255)

                                              aGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGt                                                                                                         >  2:4212841/1‑150 (MQ=255)

                                              aGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGt                                                                                                         >  2:4568558/1‑150 (MQ=255)

                                              aGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGt                                                                                                         >  1:4581030/1‑150 (MQ=255)

                                                 ctctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTaga                                                                                                      >  1:1408534/1‑150 (MQ=255)

                                                 ctctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTaga                                                                                                      >  2:1638933/1‑150 (MQ=255)

                                                  tctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTagag                                                                                                     >  2:3770058/1‑150 (MQ=255)

                                                   ctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGt                                                                                                    >  1:2155004/1‑150 (MQ=255)

                                                     ttaagttaagCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGc                                                                                                  >  2:2751832/1‑150 (MQ=255)

                                                      taagttaagCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCg                                                                                                 <  2:3004308/150‑1 (MQ=255)

                                                            aagCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCt                                                                                           >  1:2656462/1‑150 (MQ=255)

                                                             agCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCtg                                                                                          >  2:1293329/1‑150 (MQ=255)

                                                               cGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCtgtg                                                                                        >  2:4015733/1‑150 (MQ=255)

                                                                gggATACTTTATCTTTGTGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGa                                                                                       >  2:702607/1‑150 (MQ=255)

                                                                  gATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGaac                                                                                     >  1:3991/1‑150 (MQ=255)

                                                                   aTACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGaaca                                                                                    <  1:3511681/150‑1 (MQ=255)

                                                                      cTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAacaaca                                                                                 >  1:2498496/1‑150 (MQ=255)

                                                                          aTCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACg                                                                             >  2:3422780/1‑150 (MQ=255)

                                                                            cTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTc                                                                           >  2:3625235/1‑150 (MQ=255)

                                                                            cTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTc                                                                           >  2:4131712/1‑150 (MQ=255)

                                                                                gggCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCACGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGtt                                                                       <  1:4232080/150‑1 (MQ=255)

                                                                                  gCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTtta                                                                     <  1:1546958/150‑1 (MQ=255)

                                                                                  gCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTtta                                                                     >  2:2048860/1‑150 (MQ=255)

                                                                                   cTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTttat                                                                    <  1:3871527/150‑1 (MQ=255)

                                                                                    tACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTttatt                                                                   >  1:2881702/1‑150 (MQ=255)

                                                                                        cAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTc                                                               >  1:666695/1‑150 (MQ=255)

                                                                                              cAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTcc                                                         <  1:84300/150‑1 (MQ=255)

                                                                                               aGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTccc                                                        <  1:4565476/150‑1 (MQ=255)

                                                                                               aGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTccc                                                        <  1:3204399/150‑1 (MQ=255)

                                                                                                gACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCt                                                       <  2:565406/150‑1 (MQ=255)

                                                                                                gACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCt                                                       >  2:4627128/1‑150 (MQ=255)

                                                                                                  ctggATGTTTATATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCttt                                                     <  1:910750/148‑1 (MQ=255)

                                                                                                   aGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCACTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCtttt                                                    <  2:3414166/150‑1 (MQ=255)

                                                                                                   aGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCtttt                                                    >  2:933403/1‑150 (MQ=255)

                                                                                                     gATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTAt                                                  <  2:666245/150‑1 (MQ=255)

                                                                                                        gTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGc                                               <  1:2811316/150‑1 (MQ=255)

                                                                                                             tATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTtga                                          <  2:93140/150‑1 (MQ=255)

                                                                                                             tATGACTAAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCCAGCCACTGGAATCAGGCGCATTTGAACAGGATTAATCCGTAGAGTGCGATTGCTGTGAACAACAGTCGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTtga                                          <  1:3113505/150‑1 (MQ=255)

                                                                                                                gACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTtgatga                                       <  2:3108352/150‑1 (MQ=255)

                                                                                                                 aCTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGaa                                      <  1:1578262/150‑1 (MQ=255)

                                                                                                                 aCTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGaa                                      <  2:2548155/150‑1 (MQ=255)

                                                                                                                     aaaaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTg                                  >  1:3802661/1‑150 (MQ=255)

                                                                                                                      aaaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                 >  2:198431/1‑150 (MQ=255)

                                                                                                                      aaaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGa                                 <  1:2987474/150‑1 (MQ=255)

                                                                                                                       aaTATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGaa                                >  2:1546745/1‑150 (MQ=255)

                                                                                                                        atatCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAAc                               <  1:3689811/150‑1 (MQ=255)

                                                                                                                        atatCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAAc                               <  1:4465885/150‑1 (MQ=255)

                                                                                                                           tCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtc                            <  1:1145250/150‑1 (MQ=255)

                                                                                                                           tCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtc                            <  2:1861445/150‑1 (MQ=255)

                                                                                                                           tCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtc                            >  2:2583283/1‑150 (MQ=255)

                                                                                                                             aGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtctc                          >  1:2012783/1‑150 (MQ=255)

                                                                                                                             aGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtctc                          >  2:2334285/1‑150 (MQ=255)

                                                                                                                               gCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTg                        <  1:918783/150‑1 (MQ=255)

                                                                                                                               gCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTg                        <  2:1265028/150‑1 (MQ=255)

                                                                                                                                 cGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTc                      >  2:3744795/1‑150 (MQ=255)

                                                                                                                                 cGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTc                      >  2:3843023/1‑150 (MQ=255)

                                                                                                                                  gTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                     >  1:4362828/1‑150 (MQ=255)

                                                                                                                                   ttACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCg                    >  2:1521173/1‑150 (MQ=255)

                                                                                                                                    tACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgt                   <  1:1741171/150‑1 (MQ=255)

                                                                                                                                     aCCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtg                  >  2:1803072/1‑150 (MQ=255)

                                                                                                                                      ccccAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtgg                 <  1:2267846/150‑1 (MQ=255)

                                                                                                                                        ccAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtggtg               <  2:4114013/150‑1 (MQ=255)

                                                                                                                                          aaTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTa             >  2:247220/1‑150 (MQ=255)

                                                                                                                                          aaTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTa             >  2:749599/1‑150 (MQ=255)

                                                                                                                                           aTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTAt            <  2:2667225/150‑1 (MQ=255)

                                                                                                                                           aTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTAt            >  2:4492347/1‑150 (MQ=255)

                                                                                                                                             tCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTg          >  1:2683646/1‑150 (MQ=255)

                                                                                                                                             tCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTg          >  1:2130829/1‑150 (MQ=255)

                                                                                                                                                   atcatcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACg    <  1:2751809/150‑1 (MQ=255)

                                                                                                                                                     catcCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGt  <  1:1290204/150‑1 (MQ=255)

GAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGT  >  CP009273/3891272‑3891570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`