Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,925,420 A→T 31.2% T249S (ACC→TCC)  kup → potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,925,4200AT31.2% 428.8 / 274.4 276T249S (ACC→TCC) kuppotassium transporter
Reads supporting (aligned to +/- strand):  ref base A (103/87);  new base T (38/48);  total (141/135)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 276 total aligned reads displayed.

CCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGG  >  CP009273/3925273‑3925567
                                                                                                                                                   |                                                                                                                                                   
ccAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTcc                                                                                                                                                   <  2:2812004/150‑1 (MQ=255)
ccAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAcc                                                                                                                                                   <  2:3224059/150‑1 (MQ=255)
 cAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcg                                                                                                                                                  >  2:3138419/1‑150 (MQ=255)
       gggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAt                                                                                                                                            >  1:2646310/1‑150 (MQ=255)
       gggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATGACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTAt                                                                                                                                            <  2:3599107/150‑1 (MQ=255)
             tGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAcctt                                                                                                                                      <  2:1538200/150‑1 (MQ=255)
               cATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTAccttcc                                                                                                                                    <  2:518007/150‑1 (MQ=255)
               cATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcc                                                                                                                                    <  2:4423885/150‑1 (MQ=255)
                  ttcttcCTTGAATACAAAACGGTTTCTTTTATCGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTg                                                                                                                                 <  1:3173011/150‑1 (MQ=255)
                  ttattcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTg                                                                                                                                 >  2:590106/4‑150 (MQ=255)
                   tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGa                                                                                                                                <  1:792787/150‑1 (MQ=255)
                     ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGAcc                                                                                                                              <  2:4547512/150‑1 (MQ=255)
                     ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGAcc                                                                                                                              <  1:4121257/150‑1 (MQ=255)
                      tcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGAccc                                                                                                                             >  2:1809833/1‑150 (MQ=255)
                       ccTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCt                                                                                                                            <  1:3406781/150‑1 (MQ=255)
                        cTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCtt                                                                                                                           >  2:4007536/1‑150 (MQ=255)
                        cTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCtt                                                                                                                           <  1:1282437/150‑1 (MQ=255)
                         ttGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTa                                                                                                                          <  1:1845637/150‑1 (MQ=255)
                            aaTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAAtt                                                                                                                       >  1:2308874/1‑150 (MQ=255)
                              tACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTAc                                                                                                                     >  2:3759069/1‑150 (MQ=255)
                              tACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTAc                                                                                                                     >  2:1852990/1‑150 (MQ=255)
                               aCAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTAca                                                                                                                    >  1:1645781/1‑149 (MQ=255)
                                 aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTc                                                                                                                  >  1:3788506/1‑150 (MQ=255)
                                 aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTc                                                                                                                  <  2:1435943/150‑1 (MQ=255)
                                    aCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGt                                                                                                               <  2:2932996/150‑1 (MQ=255)
                                    aCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGt                                                                                                               <  1:611669/150‑1 (MQ=255)
                                    aCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGt                                                                                                               >  1:2444997/1‑150 (MQ=255)
                                        tttCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAgg                                                                                                           <  1:150056/150‑1 (MQ=255)
                                        tttCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAgg                                                                                                           >  2:376640/1‑150 (MQ=255)
                                        tttCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAgg                                                                                                           >  2:1424920/1‑150 (MQ=255)
                                         ttCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAggg                                                                                                          >  2:4644681/1‑150 (MQ=255)
                                             tttATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAgcg                                                                                                      <  1:3665123/150‑1 (MQ=255)
                                              ttATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAgcgc                                                                                                     >  1:1411802/1‑150 (MQ=255)
                                              ttATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAgcgc                                                                                                     <  2:3594342/150‑1 (MQ=255)
                                              ttATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATGACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAgcgc                                                                                                     <  2:1640864/150‑1 (MQ=255)
                                                aTTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCtg                                                                                                   >  1:1604904/1‑150 (MQ=255)
                                                 ttGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTGGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCtgt                                                                                                  >  2:1727539/1‑150 (MQ=255)
                                                  tGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCtgtt                                                                                                 <  2:971750/150‑1 (MQ=255)
                                                  tGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCtgtt                                                                                                 >  1:2927223/1‑150 (MQ=255)
                                                    cATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCtgttgt                                                                                               <  2:4335100/150‑1 (MQ=255)
                                                     aTTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTgttgtt                                                                                              <  2:1183891/150‑1 (MQ=255)
                                                     aTTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTgttgtt                                                                                              >  1:415822/1‑150 (MQ=255)
                                                       tAGGGGCAGTGGTGTTGTCGATAACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATACGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTaa                                                                                            >  1:1919455/1‑150 (MQ=255)
                                                             cAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAAcc                                                                                      <  2:346739/150‑1 (MQ=255)
                                                              aGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAAccc                                                                                     <  2:329405/150‑1 (MQ=255)
                                                              aGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAAccc                                                                                     >  1:4076503/1‑150 (MQ=255)
                                                               gtggtgTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCg                                                                                    >  2:2221324/1‑150 (MQ=255)
                                                                   tgttgtCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAg                                                                                <  2:4552807/150‑1 (MQ=255)
                                                                   tgttgtCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAg                                                                                <  1:3030594/150‑1 (MQ=255)
                                                                        tCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGAtt                                                                           >  1:1655379/1‑150 (MQ=255)
                                                                         cGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTa                                                                          >  2:690892/1‑150 (MQ=255)
                                                                          gATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTaa                                                                         >  1:4350137/1‑150 (MQ=255)
                                                                          gATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTaa                                                                         >  1:2200867/1‑150 (MQ=255)
                                                                          gATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTaa                                                                         >  1:827121/1‑150 (MQ=255)
                                                                           aTTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAg                                                                        >  2:888397/1‑150 (MQ=255)
                                                                               cGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAAcc                                                                    <  2:2507947/150‑1 (MQ=255)
                                                                                gggggTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAAccc                                                                   >  2:337678/1‑150 (MQ=255)
                                                                                gggggTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAAccc                                                                   >  2:341296/1‑150 (MQ=255)
                                                                                   ggTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGtt                                                                <  1:1584579/150‑1 (MQ=255)
                                                                                     tCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGttct                                                              <  1:997150/150‑1 (MQ=255)
                                                                                      cGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGttctt                                                             <  1:1014985/150‑1 (MQ=255)
                                                                                       gAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCACTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGttcttc                                                            >  2:2087907/1‑150 (MQ=255)
                                                                                       gAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGttcttc                                                            >  1:4578080/1‑150 (MQ=255)
                                                                                         ggCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                          >  2:3922665/1‑150 (MQ=255)
                                                                                          gcgcTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTg                                                         <  1:4050563/150‑1 (MQ=255)
                                                                                          gcgcTGTATGCTGATATGGGGATCTTTGGTAAGTTCCCTATTCGCCTGGCTTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCTGTCAGGGAGCTCTGTTGTTAAAGAACCCGGAAGCGATTAAGATCCCGTTCTTCCTg                                                         <  2:428411/150‑1 (MQ=255)
                                                                                           cgcTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGt                                                        >  2:1331072/1‑150 (MQ=255)
                                                                                           cgcTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGt                                                        <  2:3926694/150‑1 (MQ=255)
                                                                                            gcTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGtt                                                       <  2:3062092/150‑1 (MQ=255)
                                                                                             cTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTg                                                      >  2:226209/1‑150 (MQ=255)
                                                                                              tGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTgg                                                     >  2:3275734/1‑150 (MQ=255)
                                                                                               gTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                    >  2:2107810/1‑150 (MQ=255)
                                                                                               gTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                    >  2:1693548/1‑150 (MQ=255)
                                                                                               gTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                    >  2:1756795/1‑150 (MQ=255)
                                                                                                tATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCa                                                   >  2:1426799/1‑150 (MQ=255)
                                                                                                tATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCa                                                   >  1:1197207/1‑150 (MQ=255)
                                                                                                  tGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAcc                                                 <  1:3893761/150‑1 (MQ=255)
                                                                                                   gCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCg                                                <  2:2990563/150‑1 (MQ=255)
                                                                                                        tatGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTg                                           >  1:3255992/1‑150 (MQ=255)
                                                                                                        tatGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTg                                           >  2:1666791/1‑150 (MQ=255)
                                                                                                              gCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCt                                     <  1:963887/150‑1 (MQ=255)
                                                                                                                aCTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGa                                   >  1:3569985/1‑150 (MQ=255)
                                                                                                                aCTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGa                                   <  2:407130/150‑1 (MQ=255)
                                                                                                                 cTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGAt                                  >  2:560692/1‑150 (MQ=255)
                                                                                                                       tAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgct                            <  2:4147442/150‑1 (MQ=255)
                                                                                                                       tAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgct                            <  2:2551367/150‑1 (MQ=255)
                                                                                                                       tAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgct                            <  2:235000/150‑1 (MQ=255)
                                                                                                                          gTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgctgct                         <  1:2768219/150‑1 (MQ=255)
                                                                                                                             cccTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGat                      <  1:3500866/150‑1 (MQ=255)
                                                                                                                              ccTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGCGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGatc                     >  1:3973602/1‑150 (MQ=255)
                                                                                                                                    cGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgcc               >  2:3637931/1‑150 (MQ=255)
                                                                                                                                          gCGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTg         <  1:1728617/150‑1 (MQ=255)
                                                                                                                                          gCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTg         <  1:2599227/150‑1 (MQ=255)
                                                                                                                                           cGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTgg        <  1:2569567/150‑1 (MQ=255)
                                                                                                                                           cGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTgg        >  2:811754/1‑150 (MQ=255)
                                                                                                                                           cGTGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTgg        >  2:1774765/1‑150 (MQ=255)
                                                                                                                                             tGGTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCg      >  1:1098047/1‑150 (MQ=255)
                                                                                                                                              ggTTCTCCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGa     <  2:154145/150‑1 (MQ=255)
                                                                                                                                              ggTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGa     >  1:3934106/1‑150 (MQ=255)
                                                                                                                                                 tCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACgg  >  2:656681/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGG  >  CP009273/3925273‑3925567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑