Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,926,618 G→T 30.1% intergenic (+74/‑93) kup → / → rbsD potassium transporter/putative cytoplasmic sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,926,6180GT30.1% 553.6 / 191.8 266intergenic (+74/‑93)kup/rbsDpotassium transporter/putative cytoplasmic sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (102/83);  new base T (37/44);  total (139/127)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGA  >  CP009273/3926470‑3926767
                                                                                                                                                    |                                                                                                                                                     
gcgcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGGTTCGAGGt                                                                                                                                                      >  1:3314556/1‑150 (MQ=255)
 cgcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGtt                                                                                                                                                     <  1:2813505/150‑1 (MQ=255)
   cTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGa                                                                                                                                                   <  2:1007023/150‑1 (MQ=255)
       gTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTGTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATcac                                                                                                                                               >  2:755972/1‑150 (MQ=255)
           aCCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACAttt                                                                                                                                           >  2:2401882/1‑150 (MQ=255)
           aCCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACAttt                                                                                                                                           >  2:2267409/1‑150 (MQ=255)
             cAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTcc                                                                                                                                         >  1:2892281/1‑150 (MQ=255)
             cAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTcc                                                                                                                                         <  1:4351965/150‑1 (MQ=255)
             cAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTcc                                                                                                                                         >  1:1586649/1‑150 (MQ=255)
               gATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGt                                                                                                                                       >  2:4442121/1‑150 (MQ=255)
                aTCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTa                                                                                                                                      >  2:2904080/1‑150 (MQ=255)
                   aaTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACg                                                                                                                                   <  1:133594/150‑1 (MQ=255)
                   aaTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGGTGATCACATTTCCGTAACg                                                                                                                                   >  1:3487833/1‑150 (MQ=255)
                   aaTTTGAAATCCCGCCAAACAGGGATATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACg                                                                                                                                   <  2:685728/150‑1 (MQ=255)
                    aTTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGt                                                                                                                                  >  2:2090281/1‑150 (MQ=255)
                       tGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCAc                                                                                                                               >  1:4637622/1‑150 (MQ=255)
                         aaaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGa                                                                                                                             >  2:453805/1‑150 (MQ=255)
                         aaaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGa                                                                                                                             >  1:4346040/1‑150 (MQ=255)
                          aaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAt                                                                                                                            >  2:1993447/1‑150 (MQ=255)
                             cccGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGt                                                                                                                         >  1:3736088/1‑150 (MQ=255)
                             cccGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGt                                                                                                                         <  2:4058358/150‑1 (MQ=255)
                                gCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGtttt                                                                                                                      >  1:3946424/1‑150 (MQ=255)
                                    aaCAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCa                                                                                                                  <  1:3792607/150‑1 (MQ=255)
                                          gTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtcag                                                                                                            <  1:2657022/150‑1 (MQ=255)
                                           ttATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATTTAAAACGTTTAGAGGTTGATAACAATTCCGTAACGTAACGATGGTTTTACCAACtcagt                                                                                                           >  1:2257122/1‑150 (MQ=255)
                                            tATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtcagtc                                                                                                          >  2:1851585/1‑150 (MQ=255)
                                            tATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTAATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtcagtc                                                                                                          >  1:120404/1‑150 (MQ=255)
                                             aTCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtcagtca                                                                                                         >  2:1875249/1‑150 (MQ=255)
                                             aTCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtcagtca                                                                                                         <  1:1773129/150‑1 (MQ=255)
                                                gAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGa                                                                                                      <  1:613923/150‑1 (MQ=255)
                                                 aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGAt                                                                                                     >  1:4024697/1‑150 (MQ=255)
                                                 aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGAt                                                                                                     <  2:2522480/150‑1 (MQ=255)
                                                 aaCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTGGATCACCTTTCCGTAACGTCACGATGGTTTTCCCCACTCAGTCAGGag                                                                                                     >  1:1579413/1‑149 (MQ=255)
                                                  aCTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTACCAACTCAGTCAGGAtt                                                                                                    >  1:2795933/1‑150 (MQ=255)
                                                   cTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTa                                                                                                   <  1:1367003/150‑1 (MQ=255)
                                                    tGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTaa                                                                                                  <  2:2189430/150‑1 (MQ=255)
                                                     gggTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATAACCATGTAAAACGTTTCGAGGTTGATCACATTTACGTAACGTCACGATGGTTTTTACAACTCAGTCAGGATTaaa                                                                                                 >  1:780324/1‑150 (MQ=255)
                                                       gTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACt                                                                                               <  1:1814672/150‑1 (MQ=255)
                                                       gTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACt                                                                                               <  2:4346172/150‑1 (MQ=255)
                                                         aCTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACtgt                                                                                             <  2:150264/150‑1 (MQ=255)
                                                         aCTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACtgt                                                                                             <  2:2391900/150‑1 (MQ=255)
                                                           tCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTgg                                                                                           <  1:2090281/150‑1 (MQ=255)
                                                            cAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTggg                                                                                          <  2:316289/150‑1 (MQ=255)
                                                             aGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGt                                                                                         <  2:1320411/150‑1 (MQ=255)
                                                             aGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGt                                                                                         >  2:1733881/1‑150 (MQ=255)
                                                             aGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGt                                                                                         >  1:2565162/1‑150 (MQ=255)
                                                             aGGTCGAAATCTAACGCCAGACGCCTCCTTGCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGt                                                                                         >  1:3893431/1‑150 (MQ=255)
                                                             aGGTCGAAATCTAACGCCAGACGCCTCCTTGCTTCATAAGGGGGCGTGTTTTTTTTCATTGTTAAACACCATTTAAAAAGTTTAGATGTTGATCACATTTAAGTAACGTCAAGATGGTTTTACCAACTCAGTCAGGATTAAAATGTGGGt                                                                                         >  1:3893168/1‑150 (MQ=255)
                                                              ggTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTc                                                                                        >  2:1529581/1‑150 (MQ=255)
                                                                  gAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCcgcg                                                                                    >  1:1719921/1‑150 (MQ=255)
                                                                       cTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACg                                                                               <  2:1653332/150‑1 (MQ=255)
                                                                        tAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGt                                                                              >  2:1451864/1‑150 (MQ=255)
                                                                        tAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGt                                                                              <  1:2997483/150‑1 (MQ=255)
                                                                          aCGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGttt                                                                            <  2:1652875/150‑1 (MQ=255)
                                                                           cGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTc                                                                           <  1:1999815/150‑1 (MQ=255)
                                                                              cAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCCTGTAAAACGTTTCGAGGTTGATCACCTTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCt                                                                        >  2:1032569/1‑150 (MQ=255)
                                                                                 aCGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGAt                                                                     <  2:858955/150‑1 (MQ=255)
                                                                                 aCGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGAt                                                                     <  1:1355556/150‑1 (MQ=255)
                                                                                    cctcctTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGga                                                                  <  2:3928268/150‑1 (MQ=255)
                                                                                      tcctTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGgaga                                                                <  2:37035/150‑1 (MQ=255)
                                                                                       cctTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGaa                                                               <  1:574718/150‑1 (MQ=255)
                                                                                              tcATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATg                                                        <  1:732375/150‑1 (MQ=255)
                                                                                              tcATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATg                                                        <  1:786904/150‑1 (MQ=255)
                                                                                               cATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTACCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCGGATGGAGAAAAAAATGa                                                       >  2:535682/1‑150 (MQ=255)
                                                                                               cATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGa                                                       >  2:2699033/1‑150 (MQ=255)
                                                                                                aTAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGaa                                                      <  1:2461263/150‑1 (MQ=255)
                                                                                                 tAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGaaa                                                     <  1:2915899/150‑1 (MQ=255)
                                                                                                   aGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCGGATGGAGAAAAAAATGaaaaa                                                   >  2:2589571/1‑150 (MQ=255)
                                                                                                   aGGGGGCGTGTTTGTTTTCATGGTTAATAACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTTGGTCAGCGCAACGTTTGGCGGTTTGAGAAAAAAATGaaaaa                                                   >  2:1169213/1‑150 (MQ=255)
                                                                                                    gggggCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATTGTTTTCCCAACTCAGTCAGGCTTAAAAAGTGGGTCAGCGAAACGTTTAGCTGATGGAGAAAAAAAAGaaaaaa                                                  >  2:307414/1‑150 (MQ=255)
                                                                                                       ggCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAggt                                               <  2:4241518/150‑2 (MQ=255)
                                                                                                       ggCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGc                                               >  2:1136709/1‑150 (MQ=255)
                                                                                                          gTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCAcc                                            <  2:3763392/150‑1 (MQ=255)
                                                                                                          gTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCAcc                                            <  2:4392654/150‑1 (MQ=255)
                                                                                                               tGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATTAAAAAAGGCACCGTTCt                                       >  1:2395641/1‑150 (MQ=255)
                                                                                                               tGTTTTCATGGTTAAACACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCt                                       <  2:3374966/150‑1 (MQ=255)
                                                                                                                  tttCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTAGGTCAGCGAAACGTTTCGCGGAGGGAGAACAAAATGAAAAAAGGCACCGTTCTTaa                                    >  2:3101896/1‑150 (MQ=255)
                                                                                                                     cATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTc                                 <  1:4110823/150‑1 (MQ=255)
                                                                                                                       tGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTg                               <  2:2612720/150‑1 (MQ=255)
                                                                                                                         gTTAATCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCGGGTGGAGAAAAAAATGAAAAAAGGCACCGGTCTTAATTATGat                             >  1:3710131/1‑150 (MQ=255)
                                                                                                                           tAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGatat                           <  1:1183249/150‑1 (MQ=255)
                                                                                                                            aaTCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTATGATAtt                          >  1:3052552/1‑150 (MQ=255)
                                                                                                                             aTCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATAttt                         <  2:2951908/150‑1 (MQ=255)
                                                                                                                              tCACCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTc                        >  2:4140931/1‑150 (MQ=255)
                                                                                                                                aCCATGTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCAt                      <  2:4476531/150‑1 (MQ=255)
                                                                                                                                aCCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAAAGTTTAGCTGATTGAGAAAAAAATGAAAAAAATCACCGTTCTTAATTATGATATTTCAt                      >  1:1874554/1‑150 (MQ=255)
                                                                                                                                  cATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCg                    <  1:3222972/150‑1 (MQ=255)
                                                                                                                                  cATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCg                    >  2:1038922/1‑150 (MQ=255)
                                                                                                                                     gTAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCGGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTAATCGGTg                 >  1:556031/1‑150 (MQ=255)
                                                                                                                                     gTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTg                 <  2:3390117/150‑1 (MQ=255)
                                                                                                                                      tAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGa                >  1:367627/1‑150 (MQ=255)
                                                                                                                                      tAAAACGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGa                <  1:350188/150‑1 (MQ=255)
                                                                                                                                        aaaCGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGAtc              <  1:1226198/150‑1 (MQ=255)
                                                                                                                                        aaaCGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTAATCGGTGAtc              >  1:628623/1‑150 (MQ=255)
                                                                                                                                          aCGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGAtctc            <  2:3314864/150‑1 (MQ=255)
                                                                                                                                           cGTTTCGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTcc           >  1:642366/1‑150 (MQ=255)
                                                                                                                                            gTTTCGAGTTTGATCACATTTCCGTAACGTCACGTTGGTTTTCCCAACTCAGTCAGGATTAATCTGTGGGTAAGAGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTccc          <  2:1214720/150‑1 (MQ=255)
                                                                                                                                                cGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGTATTAAACTGTGGTTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCt      <  1:4131664/150‑1 (MQ=255)
                                                                                                                                                cGAGTTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCt      <  2:3587662/150‑1 (MQ=255)
                                                                                                                                                    gTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGa  <  2:2735891/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCGAAACGTTTCGCTGATGGAGAAAAAAATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGA  >  CP009273/3926470‑3926767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑