Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,994,076 A→T 100% L114* (TTA→TAA)  yigE ← DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,994,0760AT95.9% 880.1 / 18.5 257L114* (TTA→TAA) yigEDUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base A (7/4);  new base T (145/101);  total (152/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 259 total aligned reads displayed.

ATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCA  >  CP009273/3993929‑3994222
                                                                                                                                                   |                                                                                                                                                  
aTCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTAc                                                                                                                                                  >  2:3020988/1‑150 (MQ=255)
  cAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGc                                                                                                                                                >  1:651923/1‑150 (MQ=255)
   aaCATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGcc                                                                                                                                               >  2:724515/1‑150 (MQ=255)
    aCATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                                                                                                                              >  2:4011981/1‑150 (MQ=255)
     cATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCAc                                                                                                                                             <  1:2136725/150‑1 (MQ=255)
      aTTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCAcc                                                                                                                                            >  1:2678341/1‑150 (MQ=255)
      aTTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCAcc                                                                                                                                            >  1:1533790/1‑150 (MQ=255)
      aTTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCAcc                                                                                                                                            >  1:3672173/1‑150 (MQ=255)
        tGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCtt                                                                                                                                          <  1:4296451/150‑1 (MQ=255)
        tGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCtt                                                                                                                                          >  1:4503610/1‑150 (MQ=255)
         ggCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTc                                                                                                                                         >  2:2940870/1‑150 (MQ=255)
          gCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTct                                                                                                                                        >  2:4105004/1‑150 (MQ=255)
          gCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTct                                                                                                                                        <  1:3432015/150‑1 (MQ=255)
           cccTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTctg                                                                                                                                       >  1:2628904/1‑150 (MQ=255)
               gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGa                                                                                                                                   >  1:4565971/1‑150 (MQ=255)
               gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGa                                                                                                                                   <  2:1629664/150‑1 (MQ=255)
                 cTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGAcc                                                                                                                                 >  2:936065/1‑150 (MQ=255)
                    cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGtt                                                                                                                              >  1:3578635/1‑150 (MQ=255)
                    cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGtt                                                                                                                              >  2:1335632/1‑150 (MQ=255)
                       cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTc                                                                                                                           <  1:1226013/150‑1 (MQ=255)
                        gCAAACTGAATCTCTTTACTGGTTTTGAATGCATCCAGACGAACGATGCCGACTTTATCACCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTACTGCTGACCGTTTTCg                                                                                                                          >  1:2717716/1‑150 (MQ=255)
                        gCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCg                                                                                                                          >  2:2771237/1‑150 (MQ=255)
                          aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGAt                                                                                                                        >  1:3195199/1‑150 (MQ=255)
                          aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGAt                                                                                                                        >  1:19595/1‑150 (MQ=255)
                          aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGAt                                                                                                                        >  2:1877778/1‑150 (MQ=255)
                              tGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTAc                                                                                                                    >  2:217565/1‑150 (MQ=255)
                                  tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc                                                                                                                <  1:2935139/150‑1 (MQ=255)
                                  tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc                                                                                                                >  1:3765520/1‑150 (MQ=255)
                                   ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc                                                                                                               <  2:1821277/150‑1 (MQ=255)
                                    tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg                                                                                                              >  1:779008/1‑150 (MQ=255)
                                    tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg                                                                                                              >  1:2841458/1‑150 (MQ=255)
                                      tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg                                                                                                            <  1:3068776/150‑1 (MQ=255)
                                       ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc                                                                                                           <  1:679159/150‑1 (MQ=255)
                                         aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcgg                                                                                                         >  2:4457471/1‑150 (MQ=255)
                                          cTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc                                                                                                        <  1:1732788/150‑1 (MQ=255)
                                          cTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc                                                                                                        <  1:3016621/150‑1 (MQ=255)
                                           tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggcg                                                                                                       <  1:22674/150‑1 (MQ=255)
                                              ttttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAt                                                                                                    <  1:725804/150‑1 (MQ=255)
                                               tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATa                                                                                                   <  2:3514312/150‑1 (MQ=255)
                                                 tGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGc                                                                                                 >  2:1459158/1‑150 (MQ=255)
                                                 tGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGc                                                                                                 >  1:1081013/1‑150 (MQ=255)
                                                  gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCt                                                                                                >  2:3764720/1‑150 (MQ=255)
                                                    aGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCttt                                                                                              <  1:2313351/150‑1 (MQ=255)
                                                        aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatc                                                                                          <  2:3961893/150‑1 (MQ=255)
                                                            aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAg                                                                                      >  1:1773965/1‑150 (MQ=255)
                                                             gACGAACGATGCCGACATTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGa                                                                                     >  1:1893653/1‑150 (MQ=255)
                                                              acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGAt                                                                                    <  2:1634166/150‑1 (MQ=255)
                                                                gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                  >  2:606404/1‑150 (MQ=255)
                                                                 aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgcc                                                                                 >  2:3199154/1‑150 (MQ=255)
                                                                 aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgcc                                                                                 >  1:1166062/1‑150 (MQ=255)
                                                                  acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccg                                                                                >  1:287380/1‑150 (MQ=255)
                                                                    gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccgcc                                                                              >  2:2785181/1‑150 (MQ=255)
                                                                       gCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGtt                                                                           <  2:3894109/150‑1 (MQ=255)
                                                                        ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTc                                                                          >  2:4578615/1‑150 (MQ=255)
                                                                         cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCa                                                                         <  1:92171/150‑1 (MQ=255)
                                                                            cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCg                                                                      <  2:360721/150‑1 (MQ=255)
                                                                                 tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATc                                                                 >  2:4502935/1‑150 (MQ=255)
                                                                                  ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGTAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                                <  1:1055941/150‑1 (MQ=255)
                                                                                  ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                                >  2:3134076/1‑150 (MQ=255)
                                                                                  ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                                >  2:1241232/1‑150 (MQ=255)
                                                                                       gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcc                                                           <  2:4105479/150‑1 (MQ=255)
                                                                                         gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                                         <  2:1580737/150‑1 (MQ=255)
                                                                                         gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                                         >  2:1292543/1‑150 (MQ=255)
                                                                                         gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                                         >  2:2525792/1‑150 (MQ=255)
                                                                                           cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgac                                                       <  1:2194757/150‑1 (MQ=255)
                                                                                              aaaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccct                                                    <  1:2771237/150‑1 (MQ=255)
                                                                                                aaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctga                                                  >  2:3352673/1‑150 (MQ=255)
                                                                                                  cacGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACt                                                >  2:1083863/1‑150 (MQ=255)
                                                                                                   acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTa                                               <  2:653772/150‑1 (MQ=255)
                                                                                                       cgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTaa                                           >  1:3022816/1‑150 (MQ=255)
                                                                                                        gccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAat                                          <  2:3912343/150‑1 (MQ=255)
                                                                                                         ccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAGACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAata                                         <  2:2607197/150‑1 (MQ=255)
                                                                                                         ccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAata                                         <  1:359450/150‑1 (MQ=255)
                                                                                                           aGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATc                                       <  2:4317034/150‑1 (MQ=255)
                                                                                                           aGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATc                                       >  2:2980110/1‑150 (MQ=255)
                                                                                                              aCGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGc                                    <  1:4219332/150‑1 (MQ=255)
                                                                                                                ggATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCca                                  >  1:2746099/1‑150 (MQ=255)
                                                                                                                ggATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCca                                  >  1:337794/1‑150 (MQ=255)
                                                                                                                     aaGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCaga                             <  1:1871549/150‑1 (MQ=255)
                                                                                                                      aGAAATTCCCTTCACCTGAAGCGAGAAATTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCagag                            >  1:374509/1‑150 (MQ=255)
                                                                                                                         aaTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCAt                         >  2:3095832/1‑150 (MQ=255)
                                                                                                                         aaTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCAt                         >  1:4331879/1‑150 (MQ=255)
                                                                                                                          aTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATg                        >  1:2701196/1‑150 (MQ=255)
                                                                                                                           ttCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGt                       >  2:1006110/1‑150 (MQ=255)
                                                                                                                             cccTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTaa                     >  2:2429509/1‑150 (MQ=255)
                                                                                                                              ccTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAAc                    <  1:2424327/150‑1 (MQ=255)
                                                                                                                              ccTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAAc                    <  1:1644677/150‑1 (MQ=255)
                                                                                                                               cTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACg                   <  1:135867/150‑1 (MQ=255)
                                                                                                                                 tCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGtt                 <  2:4404377/150‑1 (MQ=255)
                                                                                                                                  cACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTc                >  1:572772/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTccc              <  2:779008/150‑1 (MQ=255)
                                                                                                                                       gAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATATATGCCGCAGTTCATCGCCATCTGCACCTGACACAGACTATTAATATCCGACAGCAGAGCATGTAACTTTCCCCAc           >  1:3877004/1‑150 (MQ=255)
                                                                                                                                        aaGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACg          >  2:579136/1‑150 (MQ=255)
                                                                                                                                        aaGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACg          >  1:4124309/1‑150 (MQ=255)
                                                                                                                                         aGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGc         >  1:3861819/1‑150 (MQ=255)
                                                                                                                                         aGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCAGCCAGCAGAGCATGTAACGTTCCCCACGc         >  2:2086221/1‑150 (MQ=255)
                                                                                                                                          gCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCt        <  2:1981897/150‑1 (MQ=255)
                                                                                                                                            gagaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTc      >  2:2194338/1‑150 (MQ=255)
                                                                                                                                             agaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCg     >  2:2061516/1‑150 (MQ=255)
                                                                                                                                               aTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGcc   <  2:3261776/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCA  >  CP009273/3993929‑3994222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑