Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,999,692 G→T 12.1% D157Y (GAT→TAT)  recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,999,6920GT12.1% 1033.0 / 70.6 289D157Y (GAT→TAT) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (135/119);  new base T (16/19);  total (151/138)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 292 total aligned reads displayed.

TGATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGC  >  CP009273/3999543‑3999841
                                                                                                                                                     |                                                                                                                                                     
      cAGGCTGCCGCACCGGGCAAATTTGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTcc                                                                                                                                                 >  2:864923/1‑150 (MQ=255)
      cAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTcc                                                                                                                                                 <  2:3559237/150‑1 (MQ=255)
        ggCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGc                                                                                                                                               >  2:157498/1‑150 (MQ=255)
          cTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGccc                                                                                                                                             >  1:4069159/1‑150 (MQ=255)
           tGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCg                                                                                                                                            >  2:1004880/1‑150 (MQ=255)
           tGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCg                                                                                                                                            >  2:1731205/1‑150 (MQ=255)
            gCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCgg                                                                                                                                           >  1:2043721/1‑150 (MQ=255)
             ccGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGa                                                                                                                                          >  2:3534427/1‑150 (MQ=255)
                cACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAata                                                                                                                                       >  2:380681/1‑150 (MQ=255)
                   cGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGc                                                                                                                                    >  1:1378139/1‑150 (MQ=255)
                   cGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGc                                                                                                                                    <  1:2384560/150‑1 (MQ=255)
                     ggCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCcg                                                                                                                                  >  2:2852578/1‑150 (MQ=255)
                       cAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCcgcg                                                                                                                                <  1:2573499/150‑1 (MQ=255)
                       cAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCcgcg                                                                                                                                <  1:681882/150‑1 (MQ=255)
                         aaTTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCt                                                                                                                              <  1:597587/150‑1 (MQ=255)
                         aaTTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCt                                                                                                                              >  1:4376133/1‑150 (MQ=255)
                          aTTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTc                                                                                                                             <  1:2924719/150‑1 (MQ=255)
                          aTTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTc                                                                                                                             >  1:1168619/1‑150 (MQ=255)
                          aTTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTc                                                                                                                             <  1:2082184/150‑1 (MQ=255)
                           ttCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCg                                                                                                                            <  2:2598566/150‑1 (MQ=255)
                           ttCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCg                                                                                                                            <  1:2283902/150‑1 (MQ=255)
                           ttCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCg                                                                                                                            >  2:3963677/1‑150 (MQ=255)
                            tCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCgg                                                                                                                           >  2:4635650/1‑150 (MQ=255)
                             cGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGt                                                                                                                          >  2:1233532/1‑150 (MQ=255)
                               tCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGAGTTCCGCCCGGAATATGCCGCGCTCGGTCa                                                                                                                        <  2:445675/150‑1 (MQ=255)
                                cTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTACTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCGGTCAg                                                                                                                       <  2:1516711/150‑1 (MQ=255)
                                 tGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGt                                                                                                                      >  2:2151838/1‑150 (MQ=255)
                                  gCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCGGTCAGtt                                                                                                                     <  2:1453829/150‑1 (MQ=255)
                                   cTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTg                                                                                                                    >  2:1785360/1‑150 (MQ=255)
                                    tttATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTgc                                                                                                                   <  2:3866608/150‑1 (MQ=255)
                                        tatCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCa                                                                                                               <  2:4532318/150‑1 (MQ=255)
                                             ccccGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGt                                                                                                          >  1:3623696/1‑150 (MQ=255)
                                              cccGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGtt                                                                                                         >  1:248967/1‑150 (MQ=255)
                                               ccGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTc                                                                                                        >  2:1866956/1‑150 (MQ=255)
                                                cGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTcc                                                                                                       >  1:311712/1‑150 (MQ=255)
                                                  gAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCg                                                                                                     >  2:2771353/1‑150 (MQ=255)
                                                   aaCGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGa                                                                                                    >  1:3766274/1‑150 (MQ=255)
                                                    aCGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGAc                                                                                                   <  2:102269/150‑1 (MQ=255)
                                                    aCGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGAc                                                                                                   <  1:2820232/150‑1 (MQ=255)
                                                     cGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACg                                                                                                  <  1:3211232/150‑1 (MQ=255)
                                                          gATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGcc                                                                                             >  2:3161319/1‑150 (MQ=255)
                                                             gCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGtt                                                                                          <  2:793275/150‑1 (MQ=255)
                                                              cTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGttt                                                                                         <  1:3026338/150‑1 (MQ=255)
                                                              cTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGttt                                                                                         >  1:416402/1‑150 (MQ=255)
                                                               tGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCCATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTa                                                                                        <  2:3322675/150‑1 (MQ=255)
                                                                 gATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATg                                                                                      <  2:2091545/150‑1 (MQ=255)
                                                                 gATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATg                                                                                      >  1:888016/1‑150 (MQ=255)
                                                                      cTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCt                                                                                 >  2:551684/1‑150 (MQ=255)
                                                                       tttCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTg                                                                                >  2:915785/1‑150 (MQ=255)
                                                                          cTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGAcc                                                                             >  2:3090081/1‑150 (MQ=255)
                                                                          cTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGAcc                                                                             <  2:2677887/150‑1 (MQ=255)
                                                                           ttGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCg                                                                            <  1:1778360/150‑1 (MQ=255)
                                                                                cATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCcaca                                                                       >  1:2806381/1‑150 (MQ=255)
                                                                                cATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCcaca                                                                       >  2:3654490/1‑150 (MQ=255)
                                                                                 aTCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAg                                                                      <  1:4007398/150‑1 (MQ=255)
                                                                                  tCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGc                                                                     <  1:3841227/150‑1 (MQ=255)
                                                                                    tGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCcg                                                                   >  1:1277961/1‑150 (MQ=255)
                                                                                    tGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCcg                                                                   >  1:758648/1‑150 (MQ=255)
                                                                                      gcgcACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCcgac                                                                 <  2:4231473/150‑1 (MQ=255)
                                                                                       cgcACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCcgacg                                                                <  1:2757714/150‑1 (MQ=255)
                                                                                       cgcACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCcgacg                                                                >  1:1427196/1‑150 (MQ=255)
                                                                                          aCTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGaca                                                             <  1:380681/150‑1 (MQ=255)
                                                                                          aCTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGaca                                                             >  1:3049642/1‑150 (MQ=255)
                                                                                              gAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGAcaccac                                                         <  2:645099/150‑1 (MQ=255)
                                                                                               aaTCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCAcg                                                        >  2:3784122/1‑150 (MQ=255)
                                                                                                   cGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGcc                                                    <  1:4104387/150‑1 (MQ=255)
                                                                                                    ggTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCa                                                   <  1:51648/150‑1 (MQ=255)
                                                                                                        ttattaGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGat                                               <  2:2301204/150‑1 (MQ=255)
                                                                                                          attaGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGatat                                             <  2:2949297/150‑1 (MQ=255)
                                                                                                           ttaGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATc                                            <  1:714537/150‑1 (MQ=255)
                                                                                                               ccGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTgc                                        >  2:382686/1‑150 (MQ=255)
                                                                                                                cGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTgcg                                       >  1:3548713/1‑150 (MQ=255)
                                                                                                                    gatgaAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCctg                                   >  1:1559152/1‑150 (MQ=255)
                                                                                                                      tgaAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCctgct                                 <  1:864807/150‑1 (MQ=255)
                                                                                                                       gaAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCCGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCctgctg                                >  2:2699783/1‑150 (MQ=255)
                                                                                                                       gaAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCctgctg                                >  1:23489/1‑150 (MQ=255)
                                                                                                                        aaGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTgg                               >  2:3099413/1‑150 (MQ=255)
                                                                                                                         aGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTggg                              <  1:3534449/150‑1 (MQ=255)
                                                                                                                         aGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTggg                              <  2:2043623/150‑1 (MQ=255)
                                                                                                                            gcACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCt                           >  1:1946520/1‑150 (MQ=255)
                                                                                                                              aCTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGa                         >  2:140085/1‑150 (MQ=255)
                                                                                                                               cTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGaa                        >  1:4118506/1‑150 (MQ=255)
                                                                                                                                   aTCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGAt                    >  2:2400092/1‑150 (MQ=255)
                                                                                                                                    tctcCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATc                   <  1:1004880/150‑1 (MQ=255)
                                                                                                                                    tctcCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATc                   <  1:3044364/150‑1 (MQ=255)
                                                                                                                                    tctcCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATc                   <  1:1176089/150‑1 (MQ=255)
                                                                                                                                    tctcCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATc                   <  1:1207315/150‑1 (MQ=255)
                                                                                                                                     ctcCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATcc                  >  2:4126750/1‑150 (MQ=255)
                                                                                                                                     ctcCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATcc                  <  1:2024332/150‑1 (MQ=255)
                                                                                                                                      tcCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCg                 >  1:4458761/1‑150 (MQ=255)
                                                                                                                                       cccAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGc                <  1:1711436/150‑1 (MQ=255)
                                                                                                                                       cccAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGc                <  1:4041072/150‑1 (MQ=255)
                                                                                                                                         cAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTg              >  2:3841624/1‑150 (MQ=255)
                                                                                                                                         cAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCCGCTGGGGCTGAACGATCCGCTg              <  1:3454321/150‑1 (MQ=255)
                                                                                                                                          aaTGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGa             <  2:1987413/150‑1 (MQ=255)
                                                                                                                                          aaTGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGa             <  2:3708900/150‑1 (MQ=255)
                                                                                                                                          aaTGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGa             >  1:686708/1‑150 (MQ=255)
                                                                                                                                           aTGGGGCCACTATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGAt            >  2:2479768/1‑150 (MQ=255)
                                                                                                                                           aTGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGAt            <  2:723344/150‑1 (MQ=255)
                                                                                                                                                  cACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATc     >  1:1349294/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGCATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCGGAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACACCACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGC  >  CP009273/3999543‑3999841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑