Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,050,102 G→T 12.8% A431A (GCC→GCA)  glnA ← glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,050,1020GT12.8% 1038.8 / 81.1 297A431A (GCC→GCA) glnAglutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (123/136);  new base T (15/23);  total (138/159)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 312 total aligned reads displayed.

CAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATACC  >  CP009273/4049953‑4050251
                                                                                                                                                     |                                                                                                                                                     
cAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCg                                                                                                                                                       <  2:2959339/150‑1 (MQ=255)
 aGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCgg                                                                                                                                                      >  2:922164/1‑150 (MQ=255)
 aGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCgg                                                                                                                                                      <  2:1999485/150‑1 (MQ=255)
 aGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCgg                                                                                                                                                      >  2:4432655/1‑150 (MQ=255)
 aGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCgg                                                                                                                                                      >  2:4432594/1‑150 (MQ=255)
 aGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGGCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCgg                                                                                                                                                      >  1:1309494/1‑150 (MQ=255)
   gCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCt                                                                                                                                                    <  2:582343/150‑1 (MQ=255)
     gAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCttt                                                                                                                                                  >  1:729215/1‑150 (MQ=255)
     gAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCttt                                                                                                                                                  >  2:2654377/1‑150 (MQ=255)
       aaaGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCa                                                                                                                                                <  1:1528745/150‑1 (MQ=255)
        aaGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAg                                                                                                                                               <  2:4287898/150‑1 (MQ=255)
        aaGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAg                                                                                                                                               >  2:234946/1‑150 (MQ=255)
          gTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGa                                                                                                                                             <  1:1924079/150‑1 (MQ=255)
          gTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGa                                                                                                                                             <  1:3991945/150‑1 (MQ=255)
          gTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCAAACGCACGCGGTCATCTACTTCGCGACGCAGAGCGATGTACGCAACAAATGCTACGACAGTGAACACGCCACCGGCTTTCAGGa                                                                                                                                             >  1:4625339/1‑150 (MQ=255)
            ttCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAAc                                                                                                                                           >  2:1188842/1‑150 (MQ=255)
             tCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACt                                                                                                                                          <  1:619844/150‑1 (MQ=255)
              ccACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTc                                                                                                                                         <  1:4293990/150‑1 (MQ=255)
              ccACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTc                                                                                                                                         <  1:3203551/150‑1 (MQ=255)
              ccACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTc                                                                                                                                         >  2:2467195/1‑150 (MQ=255)
               cACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTcg                                                                                                                                        >  1:2946547/1‑150 (MQ=255)
               cACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTcg                                                                                                                                        <  1:3293803/150‑1 (MQ=255)
                 cGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTcgcg                                                                                                                                      >  1:2633177/1‑150 (MQ=255)
                     aaCTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTcc                                                                                                                                  >  2:2503017/1‑150 (MQ=255)
                            acacTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCa                                                                                                                           <  2:3651751/150‑1 (MQ=255)
                              acTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAgt                                                                                                                         <  2:2767274/150‑1 (MQ=255)
                               cTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAgtt                                                                                                                        <  1:1470465/150‑1 (MQ=255)
                                ttAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAgttc                                                                                                                       <  1:2490047/150‑1 (MQ=255)
                                    aCGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAgttcgttc                                                                                                                   <  1:3744764/150‑1 (MQ=255)
                                     cGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCa                                                                                                                  >  2:2603270/1‑150 (MQ=255)
                                     cGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCa                                                                                                                  <  2:2241391/150‑1 (MQ=255)
                                       cTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGt                                                                                                                <  2:3438829/150‑1 (MQ=255)
                                        tGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTg                                                                                                               <  2:476903/150‑1 (MQ=255)
                                         gtagtaCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGc                                                                                                              >  1:943607/1‑150 (MQ=255)
                                          tagtaCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGct                                                                                                             >  1:3226304/1‑150 (MQ=255)
                                             taCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGcttct                                                                                                          >  1:270387/1‑150 (MQ=255)
                                             taCAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGcttcg                                                                                                          >  2:1109994/1‑149 (MQ=255)
                                               cAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCttcttc                                                                                                        <  1:1566633/150‑1 (MQ=255)
                                                  cTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGCGCTTCTTCCag                                                                                                     >  1:3636080/1‑150 (MQ=255)
                                                    cAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCagag                                                                                                   >  1:508412/1‑150 (MQ=255)
                                                        ctctACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGcc                                                                                               >  1:3134672/1‑150 (MQ=255)
                                                           tACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGc                                                                                            <  1:845012/150‑1 (MQ=255)
                                                               ggATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAAcc                                                                                        >  2:1514115/1‑150 (MQ=255)
                                                                 aTGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGTTGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCtg                                                                                      <  1:4254856/150‑1 (MQ=255)
                                                                 aTGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCtg                                                                                      >  1:3728380/1‑150 (MQ=255)
                                                                  tGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCtgt                                                                                     <  1:2292046/150‑1 (MQ=255)
                                                                    cGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTgg                                                                                   <  2:687678/150‑1 (MQ=255)
                                                                     ggAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTggg                                                                                  <  2:806024/150‑1 (MQ=255)
                                                                         tCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGAtct                                                                              >  2:2262404/1‑150 (MQ=255)
                                                                          cATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGAtctc                                                                             <  1:3923009/150‑1 (MQ=255)
                                                                          cATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGAtctc                                                                             <  2:1656148/150‑1 (MQ=255)
                                                                           aTACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATctct                                                                            >  2:1336111/1‑150 (MQ=255)
                                                                               gcacgcGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCg                                                                        <  2:749721/150‑1 (MQ=255)
                                                                                    cgGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGcttct                                                                   >  1:352803/1‑150 (MQ=255)
                                                                                    cgGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGcttct                                                                   <  1:2983370/150‑1 (MQ=255)
                                                                                     ggTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGcttctt                                                                  >  2:3909584/1‑150 (MQ=255)
                                                                                       tCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTtcttct                                                                <  1:1004148/150‑1 (MQ=255)
                                                                                       tCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTtcttct                                                                <  1:2630976/150‑1 (MQ=255)
                                                                                        cATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTg                                                               >  1:683915/1‑150 (MQ=255)
                                                                                         aTCTTCTTCGCGACGCAGAGCGATGTACGCATCAAGTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTgg                                                              <  1:1418168/150‑1 (MQ=255)
                                                                                           cttcttCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTggcg                                                            <  2:2522445/150‑1 (MQ=255)
                                                                                                    gACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCAt                                                   >  1:2117587/1‑150 (MQ=255)
                                                                                                      cGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATAc                                                 >  1:1145443/1‑150 (MQ=255)
                                                                                                      cGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATAc                                                 <  1:336379/150‑1 (MQ=255)
                                                                                                        cAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAg                                               >  1:1854673/1‑150 (MQ=255)
                                                                                                         agagCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAgg                                              >  2:3465115/1‑150 (MQ=255)
                                                                                                         agagCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAgg                                              >  2:1547734/1‑150 (MQ=255)
                                                                                                             cGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGtttt                                          <  1:1358111/150‑1 (MQ=255)
                                                                                                             cGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGtttt                                          <  2:2092935/150‑1 (MQ=255)
                                                                                                             cGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGtttt                                          >  1:2830687/1‑150 (MQ=255)
                                                                                                              gATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGttttt                                         <  1:3857665/150‑1 (MQ=255)
                                                                                                               aTGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTg                                        >  1:4608812/1‑150 (MQ=255)
                                                                                                               aTGTACGCAACAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCACATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATAAAGAttttag                                        >  2:3353129/1‑148 (MQ=255)
                                                                                                                tGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGt                                       <  1:4132069/150‑1 (MQ=255)
                                                                                                                  tACGCATCAATTGCTTCGTCAGTGAACACGCCACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTcc                                     >  2:3297253/1‑150 (MQ=255)
                                                                                                                    cGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCAt                                   <  1:3349465/150‑1 (MQ=255)
                                                                                                                     gCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGTGGCAGGTCATACAGGTTTTTGTCCATg                                  <  2:4625339/150‑1 (MQ=255)
                                                                                                                     gCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATg                                  >  1:2173672/1‑150 (MQ=255)
                                                                                                                     gCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATg                                  >  2:2734947/1‑150 (MQ=255)
                                                                                                                      cATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATgg                                 <  2:3152183/150‑1 (MQ=255)
                                                                                                                      cATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATgg                                 <  2:2321704/150‑1 (MQ=255)
                                                                                                                        tCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCt                               <  2:4455219/150‑1 (MQ=255)
                                                                                                                         cAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCtt                              <  2:4105450/150‑1 (MQ=255)
                                                                                                                         cAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCtt                              <  2:1443734/150‑1 (MQ=255)
                                                                                                                             tGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGcc                          <  1:4365881/150‑1 (MQ=255)
                                                                                                                                ttCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCgg                       >  2:3797877/1‑150 (MQ=255)
                                                                                                                                  cGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggat                     <  1:2503393/150‑1 (MQ=255)
                                                                                                                                  cGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggat                     <  2:3076940/150‑1 (MQ=255)
                                                                                                                                  cGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggat                     >  1:1900346/1‑150 (MQ=255)
                                                                                                                                   gTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggatg                    <  2:728936/150‑1 (MQ=255)
                                                                                                                                   gTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggatg                    >  1:983764/1‑150 (MQ=255)
                                                                                                                                   gTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggatg                    >  2:1800119/1‑150 (MQ=255)
                                                                                                                                    tCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggatgg                   >  2:4045112/1‑150 (MQ=255)
                                                                                                                                      aGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCggatggat                 >  2:4027737/1‑150 (MQ=255)
                                                                                                                                       gTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATc                <  1:1188842/150‑1 (MQ=255)
                                                                                                                                        tGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGTAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCt               <  1:1215611/150‑1 (MQ=255)
                                                                                                                                                ccACCTGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCAGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTATTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTg       >  2:2686156/1‑150 (MQ=255)
                                                                                                                                                 cACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGa      >  1:1446289/1‑150 (MQ=255)
                                                                                                                                                  aCCTGCTTTCCGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGAt     <  2:2051444/150‑1 (MQ=255)
                                                                                                                                                     ggCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATAcc  >  1:3576523/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGATCTCTTTCGCTTCTTCTGGCGGCAGGTCATACAGGTTTTTGTCCATGGCTTCGCCCGGATGGATCTTGTTCTTGATACC  >  CP009273/4049953‑4050251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑