Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,066,133 T→G 100% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,066,1330TG97.7% 1119.0 / 4.0 283S267R (AGC→CGC) yihUgamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (3/5);  new base G (146/129);  total (149/134)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 287 total aligned reads displayed.

ATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG  >  CP009273/4065986‑4066281
                                                                                                                                                   |                                                                                                                                                    
aTGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCgat                                                                                                                                                    <  1:4385099/150‑1 (MQ=255)
  gTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATaa                                                                                                                                                  <  1:1017532/150‑1 (MQ=255)
   tCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATaaa                                                                                                                                                 >  2:2203861/1‑150 (MQ=255)
    cGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAc                                                                                                                                                >  2:3745113/1‑150 (MQ=255)
    cGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTGACATTTTTAATTTGGCAGTCAACCCGGCACAGACACGGACCAGTTACAGAATGGCGGACCACTACAGGCGACCGCGACCCGCTGCGCGCCCCTGGCGATaaaa                                                                                                                                                >  1:2112133/1‑149 (MQ=255)
     gTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCATCCCGGCACTGACACGGACCTGTTACAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCAGCGCGCGCCTGGCGATAAAcc                                                                                                                                               >  2:2740185/1‑150 (MQ=255)
       tGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCATCCCGGCACAGACACCGACCTGTTCCAGAATGGCGGACCAGTCCAGGCGAACGCGACCCGCGGCGCGCGCCTGGCGATAAACCta                                                                                                                                             >  1:1405877/1‑149 (MQ=255)
        gATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGACCTGGCGACCGCGACCCGCTGCGCGCGCCAGGCGATAAACCTcc                                                                                                                                            >  2:3433914/1‑150 (MQ=255)
        gATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTGGCAGTCATCCCGGCACTGAAACGGACCTGTTCCAGAATGGCGGACCAGTACTGGCGACCGCGACCCGCGGCGCGCGCCTGGCGATAAACCTcc                                                                                                                                            >  2:3926627/1‑150 (MQ=255)
                aCTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAggc                                                                                                                                    <  2:4453099/150‑1 (MQ=255)
                 cTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATACCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTACCGTGAggcg                                                                                                                                   >  2:3458587/1‑150 (MQ=255)
                  ttATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCGGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCACCCGTGAggcgg                                                                                                                                  >  2:3669346/1‑150 (MQ=255)
                   tATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCAGGCGATAAACCACCCGTGAtgcggc                                                                                                                                 >  1:754126/1‑150 (MQ=255)
                    attcattcATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATACCGGCACAGACACGGACCTGTTACAGAATGGCGGAACAGTCCTGGCGACCGCGACCCGCGGCGCGCGCCTGGCGATAAACCACCCCTGAGGCGGcc                                                                                                                                >  1:451291/1‑150 (MQ=255)
                     ttcattcaTTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGccc                                                                                                                               <  1:3427091/150‑1 (MQ=255)
                       cattcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTTTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCAGGCGATAAACCTCCCGTGAGGCGGccccc                                                                                                                             >  2:1611095/1‑150 (MQ=255)
                          tcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCAGc                                                                                                                          >  1:2302168/1‑150 (MQ=255)
                               tATTCCTTTATCCAGTCGTTACAATTTTACTTTTGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAt                                                                                                                     >  1:4234646/1‑150 (MQ=255)
                               tATTCCTTTATCCAGTCGTTACAATTTTAATTTTGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCCCAGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCACCGGCAt                                                                                                                     >  1:2927068/1‑150 (MQ=255)
                                aTTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATNCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGNGGCCCCCNGCGGCAtn                                                                                                                    <  2:187451/150‑2 (MQ=255)
                                aTTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAtt                                                                                                                    <  2:2964454/150‑1 (MQ=255)
                                aTTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAtt                                                                                                                    <  1:4186932/150‑1 (MQ=255)
                                aTTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCAGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCAGCGGCAtt                                                                                                                    >  1:2587794/1‑150 (MQ=255)
                                aTTCCTTTATCCAGTCGTTACATTTTTAATTTGGCAGTCATCCCGGCACTGACACGGACCAGTTCCAGAATTGCGGACCAGTCCAGGCGACCGCGACCCGCGGCGCGCGCCTGGCGATAAACCACCCCTGATGCGGCCCCCCCCGGCaat                                                                                                                    >  2:1170762/1‑148 (MQ=255)
                                 ttCCTTTATCCAGTCGTTACATTTTTACATTGGCAGTCAACCCCGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCGGCGCGCGCCAGGCGATAAACCACCCGTGAGGCGGCCCCCAGCGGCAATg                                                                                                                   >  1:1431063/1‑150 (MQ=255)
                                 ttCCTTTATCCAGTCGTTACAATTTTAATTTGGCAGTCATACCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCCCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCAGCGGCATTg                                                                                                                   >  1:4049578/1‑150 (MQ=255)
                                   ccTTTCTCCAGTCTTGACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTTGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGc                                                                                                                 <  1:4538305/150‑1 (MQ=255)
                                   ccTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGc                                                                                                                 <  1:4169730/150‑1 (MQ=255)
                                   ccTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACAGACACGGACCTGTTACAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCGGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGc                                                                                                                 >  1:3477133/1‑150 (MQ=255)
                                          ccAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCa                                                                                                          <  2:4148904/150‑1 (MQ=255)
                                           cAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                         <  2:2680228/150‑1 (MQ=255)
                                           cAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                         <  2:304518/150‑1 (MQ=255)
                                             gTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCt                                                                                                       >  1:669131/1‑150 (MQ=255)
                                              tCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTg                                                                                                      >  2:771306/1‑150 (MQ=255)
                                                  tACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTg                                                                                                  >  2:4544501/1‑150 (MQ=255)
                                                   aCATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTgg                                                                                                 <  2:847295/150‑1 (MQ=255)
                                                    cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGc                                                                                                <  1:2879691/150‑1 (MQ=255)
                                                     aTTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                               >  1:3845764/1‑150 (MQ=255)
                                                     aTTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                               <  1:3882149/150‑1 (MQ=255)
                                                      tttttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGa                                                                                              >  1:4419690/1‑150 (MQ=255)
                                                        tttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACa                                                                                            >  1:2247032/1‑150 (MQ=255)
                                                        tttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACa                                                                                            >  2:4295633/1‑150 (MQ=255)
                                                         ttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACAt                                                                                           >  1:2627239/1‑150 (MQ=255)
                                                          tACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATc                                                                                          >  1:3140747/1‑150 (MQ=255)
                                                               tGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAggg                                                                                     <  2:326910/150‑1 (MQ=255)
                                                                ggCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGc                                                                                    >  1:3963998/1‑150 (MQ=255)
                                                                   aGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGAt                                                                                 >  2:1718169/1‑150 (MQ=255)
                                                                    gTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATg                                                                                <  1:4107892/150‑1 (MQ=255)
                                                                    gTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATg                                                                                >  2:1410355/1‑150 (MQ=255)
                                                                      cATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGcc                                                                              >  2:1114700/1‑150 (MQ=255)
                                                                         cccGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAg                                                                           >  2:4491408/1‑150 (MQ=255)
                                                                           cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAt                                                                         <  1:520870/150‑1 (MQ=255)
                                                                            ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                        <  2:237343/150‑1 (MQ=255)
                                                                            ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                        >  1:4090888/1‑150 (MQ=255)
                                                                               aCTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCCAGATCCtt                                                                     >  2:440141/1‑150 (MQ=255)
                                                                                cTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTa                                                                    <  1:874522/150‑1 (MQ=255)
                                                                                 tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                   >  2:3139688/1‑150 (MQ=255)
                                                                                 tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                   >  1:2487176/1‑150 (MQ=255)
                                                                                   acacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATgg                                                                 <  1:1729424/150‑1 (MQ=255)
                                                                                   acacGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATgg                                                                 <  1:1921838/150‑1 (MQ=255)
                                                                                       ggACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTGATGGGcca                                                             >  2:4007879/1‑148 (MQ=255)
                                                                                        gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAg                                                            >  2:1012097/1‑150 (MQ=255)
                                                                                          ccTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgat                                                          >  2:4053212/1‑150 (MQ=255)
                                                                                           ctcttCAAGAATGGCTGACCAGTCCTGGCGACCGCGACACGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGAGGAACCAAGCGGCATTGGAACAAGCATCTGGTTGGAGACATCAAGGGAGATGACAAGATCCTTATGGGCCAgatc                                                         >  1:2074148/4‑150 (MQ=255)
                                                                                           cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                         >  2:2166617/1‑150 (MQ=255)
                                                                                            tGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcg                                                        >  1:2987679/1‑150 (MQ=255)
                                                                                            tGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcg                                                        <  2:2322511/150‑1 (MQ=255)
                                                                                             gTTCCAGATTGGCGGAACAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcga                                                       <  1:1775791/150‑1 (MQ=255)
                                                                                               tCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcgatc                                                     >  2:3655860/1‑150 (MQ=255)
                                                                                                ccAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCa                                                    <  2:2853116/150‑1 (MQ=255)
                                                                                                 cAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCAt                                                   >  2:2515553/1‑150 (MQ=255)
                                                                                                   gAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGa                                                 <  1:2271800/150‑1 (MQ=255)
                                                                                                   gAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGa                                                 <  1:277779/150‑1 (MQ=255)
                                                                                                           gACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGga                                         <  1:1541676/150‑1 (MQ=255)
                                                                                                           gACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGga                                         <  2:4623157/150‑1 (MQ=255)
                                                                                                                 tCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGa                                   <  2:3872349/150‑1 (MQ=255)
                                                                                                                 tCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGa                                   <  1:3557688/150‑1 (MQ=255)
                                                                                                                  ccTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTCTGGGCACGATCGATCATGAAGGCGGGAGAAAGAt                                  >  2:1198751/1‑150 (MQ=255)
                                                                                                                  ccTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAt                                  >  1:1941051/1‑150 (MQ=255)
                                                                                                                  ccTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAt                                  >  1:1641930/1‑150 (MQ=255)
                                                                                                                  ccTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAt                                  >  1:3225356/1‑150 (MQ=255)
                                                                                                                   cTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtc                                 >  2:2935465/1‑150 (MQ=255)
                                                                                                                    tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGAtct                                >  1:3902895/1‑150 (MQ=255)
                                                                                                                    tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                                >  1:1838420/1‑150 (MQ=255)
                                                                                                                       cgaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCg                             <  1:1008095/150‑1 (MQ=255)
                                                                                                                        gaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGc                            <  1:2400022/150‑1 (MQ=255)
                                                                                                                         accgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCt                           <  1:869536/150‑1 (MQ=255)
                                                                                                                          ccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                          >  2:948809/1‑150 (MQ=255)
                                                                                                                             cgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAgg                       <  2:3455225/150‑1 (MQ=255)
                                                                                                                             cgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAgg                       >  1:1996379/1‑150 (MQ=255)
                                                                                                                              gaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGa                      >  1:1353777/1‑150 (MQ=255)
                                                                                                                                   gCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttg                 <  2:1986379/150‑1 (MQ=255)
                                                                                                                                   gCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACtttg                 <  2:754126/150‑1 (MQ=255)
                                                                                                                                        gcgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTgg            <  1:4640684/150‑1 (MQ=255)
                                                                                                                                         cgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGc           >  2:4566832/1‑150 (MQ=255)
                                                                                                                                             ccTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAgg       <  1:3493613/150‑1 (MQ=255)
                                                                                                                                             ccTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAgg       <  2:618051/150‑1 (MQ=255)
                                                                                                                                               tGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa     >  2:417609/1‑150 (MQ=255)
                                                                                                                                               tGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa     <  2:2587794/150‑1 (MQ=255)
                                                                                                                                               tGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGaa     <  2:1738379/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAGTG  >  CP009273/4065986‑4066281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑