BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,210,835	G→C	15.7%	C527W (TGC→TGG)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,210,835	0	G	C	15.7%	879.4 / 152.6	262	C527W (TGC→TGG)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (108/113); new base C (21/20); total (129/133)
Fisher's exact test for biased strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 264 total aligned reads displayed.

CCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGG  >  CP009273/4210687‑4210983

ccGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGc                                                                                                                                                     >  2:422675/1‑150 (MQ=255)

ccGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGc                                                                                                                                                     >  2:422758/1‑150 (MQ=255)

ccGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGc                                                                                                                                                     <  2:1379254/150‑1 (MQ=255)

       gcgcgcAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATa                                                                                                                                              <  1:1328691/150‑1 (MQ=255)

           gcAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATaaaaa                                                                                                                                          <  2:51618/150‑1 (MQ=255)

           gcAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATaaaaa                                                                                                                                          >  2:4510792/1‑150 (MQ=255)

            cAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAAt                                                                                                                                         <  2:2437041/150‑1 (MQ=255)

             aaGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATc                                                                                                                                        >  1:1673792/1‑150 (MQ=255)

                 cTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCtat                                                                                                                                    >  1:3936738/1‑148 (MQ=255)

                    ttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGcc                                                                                                                                 <  2:1854551/150‑1 (MQ=255)

                    ttAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGcc                                                                                                                                 >  1:2102950/1‑150 (MQ=255)

                            cAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGgag                                                                                                                         >  1:4195421/1‑150 (MQ=255)

                             agaagaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGgaga                                                                                                                        <  1:3569680/150‑1 (MQ=255)

                               aagaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCTTTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATc                                                                                                                      <  1:3455093/150‑1 (MQ=255)

                               aagaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATc                                                                                                                      <  1:2963080/150‑1 (MQ=255)

                                agaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCg                                                                                                                     >  2:3067321/1‑150 (MQ=255)

                                agaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCg                                                                                                                     <  2:3784442/150‑1 (MQ=255)

                                agaTCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCg                                                                                                                     >  2:369961/1‑150 (MQ=255)

                                     tACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTAc                                                                                                                >  1:198352/1‑150 (MQ=255)

                                     tACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTAc                                                                                                                >  1:1058238/1‑150 (MQ=255)

                                     tACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTAc                                                                                                                >  1:3676529/1‑150 (MQ=255)

                                     aaCAATATCGGAAGCTGAAATGTTAATTTCATGGGCTAGGCTGGGCAATGATTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTTCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCATTCAGGAGAACGAGTAc                                                                                                                >  2:2284871/2‑150 (MQ=255)

                                       cAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCt                                                                                                              >  1:2144517/1‑150 (MQ=255)

                                       cAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCt                                                                                                              <  1:2832580/150‑1 (MQ=255)

                                        aaTATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTg                                                                                                             >  2:391193/1‑150 (MQ=255)

                                        aaTATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTg                                                                                                             >  2:391242/1‑150 (MQ=255)

                                         atatCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGt                                                                                                            >  1:3364145/1‑150 (MQ=255)

                                           atCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTc                                                                                                          >  1:2167833/1‑150 (MQ=255)

                                            tCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCt                                                                                                         <  2:1216673/150‑1 (MQ=255)

                                             cGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                                                                                                        >  1:2250142/1‑150 (MQ=255)

                                             cGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCtt                                                                                                        <  2:3041762/150‑1 (MQ=255)

                                              ggAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCttt                                                                                                       >  2:2063213/1‑150 (MQ=255)

                                                aaGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAg                                                                                                     <  1:755220/150‑1 (MQ=255)

                                                    tGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaa                                                                                                 <  1:3715903/150‑1 (MQ=255)

                                                     gAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaaa                                                                                                <  2:792558/150‑1 (MQ=255)

                                                     gAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCaaa                                                                                                >  2:3254105/1‑150 (MQ=255)

                                                       aaTGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATg                                                                                              <  2:449545/150‑1 (MQ=255)

                                                         tGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGAc                                                                                            >  1:2443817/1‑150 (MQ=255)

                                                         tGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGAc                                                                                            <  2:2650151/150‑1 (MQ=255)

                                                         tGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGAc                                                                                            >  1:33219/1‑150 (MQ=255)

                                                           ttAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAt                                                                                          <  2:2324155/150‑1 (MQ=255)

                                                            tAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACAtg                                                                                         <  2:2890719/150‑1 (MQ=255)

                                                                  ccTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGaa                                                                                   >  1:1976020/1‑150 (MQ=255)

                                                                   cTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAAt                                                                                  <  1:4404347/150‑1 (MQ=255)

                                                                    tGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAAtt                                                                                 >  1:1866615/1‑150 (MQ=255)

                                                                    tGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAAtt                                                                                 <  1:4237217/150‑1 (MQ=255)

                                                                      ggCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTaa                                                                               >  2:3177947/1‑150 (MQ=255)

                                                                       gCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAAc                                                                              <  1:1185247/150‑1 (MQ=255)

                                                                       gCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAAc                                                                              <  2:1064033/150‑1 (MQ=255)

                                                                          aGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACaaa                                                                           >  2:2700011/1‑150 (MQ=255)

                                                                           ggCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAg                                                                          <  2:3927238/150‑1 (MQ=255)

                                                                                ggCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAAc                                                                     >  1:3903738/1‑150 (MQ=255)

                                                                                   aaTGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAAcgcg                                                                  >  1:3489966/1‑150 (MQ=255)

                                                                                        ttCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCaa                                                             <  1:4612804/150‑1 (MQ=255)

                                                                                           aaGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAAc                                                          <  1:2864028/150‑1 (MQ=255)

                                                                                            aGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACa                                                         <  2:2384229/150‑1 (MQ=255)

                                                                                            aGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACa                                                         <  2:2384219/150‑1 (MQ=255)

                                                                                             gAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACaa                                                        >  1:1087585/1‑150 (MQ=255)

                                                                                              aaTAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAt                                                       <  1:3814437/150‑1 (MQ=255)

                                                                                               aTAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAtt                                                      <  1:2958580/150‑1 (MQ=255)

                                                                                               aTAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAtt                                                      <  2:1637789/150‑1 (MQ=255)

                                                                                               aTAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAtt                                                      <  1:1471714/150‑1 (MQ=255)

                                                                                                tAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAttt                                                     <  2:938694/150‑1 (MQ=255)

                                                                                                tAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAttt                                                     <  2:2103126/150‑1 (MQ=255)

                                                                                                 aaCTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAAtttt                                                    <  2:3364145/150‑1 (MQ=255)

                                                                                                  aCTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTc                                                   <  2:1014671/150‑1 (MQ=255)

                                                                                                    tttCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAg                                                 >  2:3965287/1‑150 (MQ=255)

                                                                                                     ttCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGc                                                >  1:1502167/1‑150 (MQ=255)

                                                                                                     ttCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGc                                                >  1:3672727/1‑150 (MQ=255)

                                                                                                       cACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCAt                                              >  1:2007574/1‑150 (MQ=255)

                                                                                                        aCGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATg                                             <  1:3707351/150‑1 (MQ=255)

                                                                                                          gATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTc                                           <  1:2591884/150‑1 (MQ=255)

                                                                                                            tatCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCgg                                         <  1:2433482/150‑1 (MQ=255)

                                                                                                            tatCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCgg                                         >  1:271834/1‑150 (MQ=255)

                                                                                                             atCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGc                                        >  2:4146399/1‑150 (MQ=255)

                                                                                                                  tATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCAc                                   <  1:3046416/150‑1 (MQ=255)

                                                                                                                   aTGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTCACGCGTTCAAAACAATTTTCAGCATGTCGGCATCAcc                                  >  1:1628326/1‑150 (MQ=255)

                                                                                                                    tGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCa                                 >  2:1805245/1‑150 (MQ=255)

                                                                                                                     gTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCAt                                >  2:4074037/1‑150 (MQ=255)

                                                                                                                     gTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCAt                                >  2:3387739/1‑150 (MQ=255)

                                                                                                                       ccATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTc                              >  2:1436014/1‑150 (MQ=255)

                                                                                                                         aTTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGc                            >  1:4110507/1‑150 (MQ=255)

                                                                                                                         aTTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGc                            <  2:2642588/150‑1 (MQ=255)

                                                                                                                           tttGCATCGCCAGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCa                          <  1:3554618/150‑1 (MQ=255)

                                                                                                                              gCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATaa                       >  2:2207859/1‑150 (MQ=255)

                                                                                                                              gCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATaa                       >  1:1201002/1‑150 (MQ=255)

                                                                                                                                   gCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATgg                  <  1:2371610/150‑1 (MQ=255)

                                                                                                                                      aGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGtat               >  1:3152870/1‑150 (MQ=255)

                                                                                                                                      aGATATAGTCCTGGCCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGtat               >  2:3807466/1‑150 (MQ=255)

                                                                                                                                       gATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGtata              <  1:617583/150‑1 (MQ=255)

                                                                                                                                          ataGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATaaaa           >  1:3396134/1‑150 (MQ=255)

                                                                                                                                           taGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAAc          <  2:631317/150‑1 (MQ=255)

                                                                                                                                            aGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACa         >  1:2568875/1‑150 (MQ=255)

                                                                                                                                            aGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACa         >  1:1552091/1‑150 (MQ=255)

                                                                                                                                               ccTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATgg      <  2:3635193/150‑1 (MQ=255)

                                                                                                                                                cTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGc     <  2:3676529/150‑1 (MQ=255)

                                                                                                                                                 tGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCt    >  1:2487404/1‑150 (MQ=255)

                                                                                                                                                 tGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCt    >  2:3830077/1‑150 (MQ=255)

                                                                                                                                                 tGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCt    >  2:2113539/1‑150 (MQ=255)

                                                                                                                                                   ggCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTgg  >  2:2903302/1‑150 (MQ=255)

CCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGG  >  CP009273/4210687‑4210983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`