Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,230,913 A→T 100% W424R (TGG→AGG)  xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,230,9130AT97.1% 953.2 / 12.4 277W424R (TGG→AGG) xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base A (6/2);  new base T (143/126);  total (149/128)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 277 total aligned reads displayed.

CCTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATC  >  CP009273/4230766‑4231058
                                                                                                                                                   |                                                                                                                                                 
cctcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTgg                                                                                                                                                 <  2:2717730/150‑1 (MQ=255)
  tcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATACACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCTGGAGTTTTTGTCCATCATCGGGATGGTCCTGgag                                                                                                                                               <  1:646310/150‑1 (MQ=255)
  tcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGgag                                                                                                                                               >  1:2318649/1‑150 (MQ=255)
    aaGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGAc                                                                                                                                             <  2:4642517/150‑1 (MQ=255)
    aaGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGAc                                                                                                                                             <  1:2225741/150‑1 (MQ=255)
       ggTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGaa                                                                                                                                          >  2:2463491/1‑150 (MQ=255)
         ttttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagt                                                                                                                                        >  1:3026617/1‑150 (MQ=255)
         ttttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagt                                                                                                                                        <  1:1945986/150‑1 (MQ=255)
          tttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagta                                                                                                                                       >  1:2788776/1‑150 (MQ=255)
            tACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagtagt                                                                                                                                     <  1:1360140/150‑1 (MQ=255)
             aCCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGtt                                                                                                                                    <  1:2283673/150‑1 (MQ=255)
              ccTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTc                                                                                                                                   <  1:4605694/150‑1 (MQ=255)
               cTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCg                                                                                                                                  >  2:855497/1‑150 (MQ=255)
               cTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCg                                                                                                                                  >  2:4324501/1‑150 (MQ=255)
                 ttGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATAGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgcc                                                                                                                                <  2:3982821/150‑1 (MQ=255)
                  tGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgcca                                                                                                                               <  2:3785990/150‑1 (MQ=255)
                    gTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgccagc                                                                                                                             >  2:3406413/1‑150 (MQ=255)
                        ccGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACt                                                                                                                         >  2:4198322/1‑150 (MQ=255)
                         cGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTg                                                                                                                        >  1:2460235/1‑150 (MQ=255)
                            gACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgc                                                                                                                     <  1:3309190/150‑1 (MQ=255)
                            gACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgc                                                                                                                     >  2:3301076/1‑150 (MQ=255)
                             aCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGTGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgcc                                                                                                                    <  1:3753314/150‑1 (MQ=255)
                              cAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgccg                                                                                                                   >  2:3699391/1‑150 (MQ=255)
                                 aTTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCa                                                                                                                <  1:1855907/150‑1 (MQ=255)
                                    tCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCATTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCAATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcg                                                                                                             >  1:3153356/1‑150 (MQ=255)
                                        caTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAt                                                                                                         <  1:965406/150‑1 (MQ=255)
                                        caTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAt                                                                                                         >  1:2573723/1‑150 (MQ=255)
                                         aTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAtt                                                                                                        <  1:3125418/150‑1 (MQ=255)
                                         aTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAtt                                                                                                        >  2:2780721/1‑150 (MQ=255)
                                         aTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAtt                                                                                                        >  1:1368619/1‑150 (MQ=255)
                                          tAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTg                                                                                                       >  2:559841/1‑150 (MQ=255)
                                           aaaCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGc                                                                                                      <  2:4267831/150‑1 (MQ=255)
                                            aaCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGcc                                                                                                     >  1:2095709/1‑150 (MQ=255)
                                             aCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCa                                                                                                    <  2:3905342/150‑1 (MQ=255)
                                             aCAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCa                                                                                                    <  2:2992796/150‑1 (MQ=255)
                                              cAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAg                                                                                                   >  1:1001202/1‑150 (MQ=255)
                                               aGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAgc                                                                                                  >  2:2326318/1‑150 (MQ=255)
                                                gTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAgcg                                                                                                 >  1:3693331/1‑150 (MQ=255)
                                                  gctgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCt                                                                                               >  1:1047443/1‑150 (MQ=255)
                                                     gcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTa                                                                                            >  1:3896394/1‑150 (MQ=255)
                                                     gcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTa                                                                                            <  1:1293656/150‑1 (MQ=255)
                                                      ccAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTAc                                                                                           >  1:1268634/1‑150 (MQ=255)
                                                      ccAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTAc                                                                                           >  1:4268125/1‑150 (MQ=255)
                                                       cAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTAcc                                                                                          >  1:2368278/1‑150 (MQ=255)
                                                        aGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGATTTGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCa                                                                                         <  1:4183009/150‑1 (MQ=255)
                                                        aGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCa                                                                                         <  1:402997/150‑1 (MQ=255)
                                                            cGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGaa                                                                                     >  1:3669839/1‑150 (MQ=255)
                                                             gCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAAt                                                                                    >  1:1541600/1‑150 (MQ=255)
                                                             gCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAAt                                                                                    >  2:1797063/1‑150 (MQ=255)
                                                              cccATCCAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCTCCACCGCGATTGCCAGCGCTTTACCACGAATa                                                                                   <  1:916580/150‑1 (MQ=255)
                                                              cccATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATa                                                                                   <  2:2584203/150‑1 (MQ=255)
                                                              cccATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATa                                                                                   <  2:2377689/150‑1 (MQ=255)
                                                                 aTACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCa                                                                                >  1:4270708/1‑150 (MQ=255)
                                                                  tACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCAt                                                                               <  2:2629924/150‑1 (MQ=255)
                                                                    cAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTc                                                                             <  2:3093524/150‑1 (MQ=255)
                                                                          tAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAg                                                                       >  1:1400964/1‑150 (MQ=255)
                                                                           aaaTCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACTAATAGCATTCGGGAAGa                                                                      >  2:770219/1‑150 (MQ=255)
                                                                           aaaTCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGa                                                                      >  2:28405/1‑150 (MQ=255)
                                                                            aaTCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGAt                                                                     <  2:256110/150‑1 (MQ=255)
                                                                                 aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCg                                                                <  1:1381042/150‑1 (MQ=255)
                                                                                    aGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAca                                                             <  2:3593647/150‑1 (MQ=255)
                                                                                    aGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAca                                                             <  2:2172612/150‑1 (MQ=255)
                                                                                       agaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagca                                                          >  2:3633447/1‑150 (MQ=255)
                                                                                        gaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagcag                                                         <  2:3595299/150‑1 (MQ=255)
                                                                                         aaaCCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGt                                                        >  1:2890764/1‑150 (MQ=255)
                                                                                              gTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCa                                                   >  1:108949/1‑150 (MQ=255)
                                                                                                tgtgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGc                                                 >  2:4147895/1‑150 (MQ=255)
                                                                                                tgtgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGc                                                 >  2:3746819/1‑150 (MQ=255)
                                                                                                  tgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                               <  1:48229/150‑1 (MQ=255)
                                                                                                  tgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                               <  2:2894969/150‑1 (MQ=255)
                                                                                                  tgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                               >  1:3527304/1‑150 (MQ=255)
                                                                                                    gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAc                                             >  2:1437496/1‑150 (MQ=255)
                                                                                                    gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAc                                             <  2:1359801/150‑1 (MQ=255)
                                                                                                     aaaTGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCAAGCATAcc                                            >  2:795059/1‑150 (MQ=255)
                                                                                                        tGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGa                                         <  2:1996064/150‑1 (MQ=255)
                                                                                                            ccaccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGAcccc                                     <  1:266417/150‑1 (MQ=255)
                                                                                                             caccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCa                                    >  2:1687474/1‑150 (MQ=255)
                                                                                                             caccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCa                                    <  2:4533272/150‑1 (MQ=255)
                                                                                                              accaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAg                                   >  1:4499484/1‑150 (MQ=255)
                                                                                                                 agccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGAc                                >  1:1216097/1‑150 (MQ=255)
                                                                                                                    cagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATg                             >  2:2262863/1‑150 (MQ=255)
                                                                                                                    cagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATg                             >  2:1029361/1‑150 (MQ=255)
                                                                                                                     agGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATgg                            >  2:4178958/1‑150 (MQ=255)
                                                                                                                      ggAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGc                           >  1:1562081/1‑150 (MQ=255)
                                                                                                                      ggAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGc                           <  2:923391/150‑1 (MQ=255)
                                                                                                                         gTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCaaa                        <  1:1673525/150‑1 (MQ=255)
                                                                                                                          tttttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAg                       <  2:1142222/150‑1 (MQ=255)
                                                                                                                            tttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggc                     <  1:831544/150‑1 (MQ=255)
                                                                                                                             ttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcg                    <  1:11876/150‑1 (MQ=255)
                                                                                                                               gTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcggc                  <  1:1761051/150‑1 (MQ=255)
                                                                                                                                   atcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACa              >  1:487569/1‑150 (MQ=255)
                                                                                                                                    tcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACAt             >  1:694414/1‑150 (MQ=255)
                                                                                                                                    tcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACAt             >  1:3482010/1‑150 (MQ=255)
                                                                                                                                    tcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACAt             >  1:2379116/1‑150 (MQ=255)
                                                                                                                                        cGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGaa         >  1:3907862/1‑150 (MQ=255)
                                                                                                                                        cGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGaa         >  1:3373897/1‑150 (MQ=255)
                                                                                                                                         gggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAAc        >  2:124149/1‑150 (MQ=255)
                                                                                                                                            aaGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGc     >  2:319355/1‑150 (MQ=255)
                                                                                                                                             aGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCa    >  1:2045315/1‑150 (MQ=255)
                                                                                                                                              ggTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCAt   <  2:2546748/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATC  >  CP009273/4230766‑4231058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑