Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,748 C→A 58.5% R28L (CGC→CTC)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,7480CA58.5% 62.2 / inf 323R28L (CGC→CTC) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base C (68/66);  new base A (104/85);  total (172/151)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

AGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGA  >  CP009273/4274601‑4274894
                                                                                                                                                   |                                                                                                                                                  
  cccGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGctc                                                                                                                                                >  2:927173/1‑150 (MQ=255)
  cccGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGctc                                                                                                                                                >  2:4272373/1‑150 (MQ=255)
            aaGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCg                                                                                                                                      <  2:814489/150‑1 (MQ=255)
            aaGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCg                                                                                                                                      <  2:3653011/150‑1 (MQ=255)
               ccAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTa                                                                                                                                   <  1:741780/150‑1 (MQ=255)
               ccAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTa                                                                                                                                   <  1:2137586/150‑1 (MQ=255)
                     atatTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCa                                                                                                                             <  1:1238620/150‑1 (MQ=255)
                     atatTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCa                                                                                                                             >  2:3373345/1‑150 (MQ=255)
                         tGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCcg                                                                                                                         <  2:48760/150‑1 (MQ=255)
                                 gCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTa                                                                                                                 <  2:459996/150‑1 (MQ=255)
                                    gtgtAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCt                                                                                                              <  1:148928/150‑1 (MQ=255)
                                    gtgtAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCt                                                                                                              <  2:575919/150‑1 (MQ=255)
                                     tgtAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTg                                                                                                             <  1:1144420/150‑1 (MQ=255)
                                       tagtagTCGCTACGAGAACGTACTCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCg                                                                                                           >  1:4604440/1‑150 (MQ=255)
                                           agtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAg                                                                                                       >  2:1694388/1‑150 (MQ=255)
                                           agtCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAg                                                                                                       >  2:536704/1‑150 (MQ=255)
                                              cGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGa                                                                                                    >  2:3245515/1‑150 (MQ=255)
                                                     agaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggc                                                                                             <  1:2010222/150‑1 (MQ=255)
                                                     agaACGTACGAGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggc                                                                                             <  2:1298667/150‑1 (MQ=255)
                                                      gaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggcg                                                                                            <  1:2379143/150‑1 (MQ=255)
                                                      gaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggcg                                                                                            >  1:1314880/1‑150 (MQ=255)
                                                      gaACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggcg                                                                                            <  1:229621/150‑1 (MQ=255)
                                                        aCGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggcggc                                                                                          >  2:4137005/1‑150 (MQ=255)
                                                           tACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAg                                                                                       <  1:623544/150‑1 (MQ=255)
                                                            aCGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAgc                                                                                      >  1:2979354/1‑150 (MQ=255)
                                                            aCGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAgc                                                                                      >  1:2184748/1‑150 (MQ=255)
                                                              gcgTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAgcgc                                                                                    <  2:1574195/150‑1 (MQ=255)
                                                               cgTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGcc                                                                                   >  1:1011037/1‑150 (MQ=255)
                                                                    tGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATACTCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAg                                                                              <  2:3296520/150‑1 (MQ=255)
                                                                    tGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAg                                                                              >  2:2347302/1‑150 (MQ=255)
                                                                     gATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGa                                                                             <  1:156065/150‑1 (MQ=255)
                                                                      aTGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGaa                                                                            >  2:2743975/1‑150 (MQ=255)
                                                                           cAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCtt                                                                       <  2:2224756/150‑1 (MQ=255)
                                                                            aGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCttt                                                                      >  2:2688508/1‑150 (MQ=255)
                                                                             gTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCtttt                                                                     <  1:3215748/150‑1 (MQ=255)
                                                                               aGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATACTCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCa                                                                   <  1:276345/150‑1 (MQ=255)
                                                                                ggTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCat                                                                  >  1:3848307/1‑150 (MQ=255)
                                                                                  tAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCatga                                                                <  1:4040356/150‑1 (MQ=255)
                                                                                   aaTGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCatgat                                                               >  1:2448788/1‑150 (MQ=255)
                                                                                    aTGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCatgatg                                                              >  1:2226499/1‑150 (MQ=255)
                                                                                     tGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGc                                                             >  2:284017/1‑150 (MQ=255)
                                                                                     tGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGc                                                             >  2:373651/1‑150 (MQ=255)
                                                                                     tGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGGGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGc                                                             >  2:4473297/1‑150 (MQ=255)
                                                                                       ccGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCtt                                                           <  2:3188426/150‑1 (MQ=255)
                                                                                         gAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGt                                                         <  1:3352516/150‑1 (MQ=255)
                                                                                           gCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTAc                                                       <  1:2154747/150‑1 (MQ=255)
                                                                                           gCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTAc                                                       <  1:2483405/150‑1 (MQ=255)
                                                                                              tAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTc                                                    <  2:1511964/150‑1 (MQ=255)
                                                                                                aaCACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCAt                                                  >  2:72894/1‑150 (MQ=255)
                                                                                                aaCACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCAt                                                  >  1:4019655/1‑150 (MQ=255)
                                                                                                 acacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATg                                                 <  1:3640716/150‑1 (MQ=255)
                                                                                                 acacGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATg                                                 <  2:2093504/150‑1 (MQ=255)
                                                                                                     gacgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAgg                                             >  2:1269644/1‑150 (MQ=255)
                                                                                                       cgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGATAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGAc                                           <  1:1185778/150‑1 (MQ=255)
                                                                                                       cgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGAc                                           >  1:4576157/1‑150 (MQ=255)
                                                                                                       cgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGAc                                           >  2:2197191/1‑150 (MQ=255)
                                                                                                       cgaAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGAc                                           >  2:1930295/1‑150 (MQ=255)
                                                                                                         aaaaTCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACtt                                         <  1:1742579/150‑1 (MQ=255)
                                                                                                         aaaaTCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACtt                                         <  2:3454206/150‑1 (MQ=255)
                                                                                                         aaaaTCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACtt                                         <  1:1676411/150‑1 (MQ=255)
                                                                                                          aaaTCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTc                                        <  2:2579006/150‑1 (MQ=255)
                                                                                                              cAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCatta                                    <  2:1634563/150‑1 (MQ=255)
                                                                                                               aGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCattat                                   <  1:918686/150‑1 (MQ=255)
                                                                                                                 gAACATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCAttatta                                 >  1:1981757/1‑150 (MQ=255)
                                                                                                                    cATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGa                              >  1:4358797/1‑150 (MQ=255)
                                                                                                                    cATAATAATCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGa                              >  2:2367727/1‑150 (MQ=255)
                                                                                                                      taataaTCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACg                            <  1:3202675/150‑1 (MQ=255)
                                                                                                                      taataaTCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACg                            <  1:2291503/150‑1 (MQ=255)
                                                                                                                      taataaTCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACg                            <  1:3053163/150‑1 (MQ=255)
                                                                                                                        ataatCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGt                          >  2:2941101/1‑150 (MQ=255)
                                                                                                                        ataatCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGt                          <  2:101336/150‑1 (MQ=255)
                                                                                                                        ataatCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGt                          <  2:3992745/150‑1 (MQ=255)
                                                                                                                         taatCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTc                         >  2:895827/1‑150 (MQ=255)
                                                                                                                         taatCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTc                         >  1:1825149/1‑150 (MQ=255)
                                                                                                                          aatCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCg                        <  1:1355098/150‑1 (MQ=255)
                                                                                                                            tCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGaa                      >  1:3436546/1‑150 (MQ=255)
                                                                                                                            tCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGaa                      <  2:2730275/150‑1 (MQ=255)
                                                                                                                            tCGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGaa                      >  2:2664035/1‑150 (MQ=255)
                                                                                                                             cGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGCGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAt                     >  2:4043563/1‑150 (MQ=255)
                                                                                                                             cGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAt                     <  1:3083988/150‑1 (MQ=255)
                                                                                                                             cGCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAt                     >  2:4477114/1‑150 (MQ=255)
                                                                                                                              gCCTGCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                    >  1:371814/1‑150 (MQ=255)
                                                                                                                               ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTTATAGCATCCGCGGGGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCTCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTTAGTCGGTCGAATTc                   <  1:408689/150‑1 (MQ=255)
                                                                                                                               ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                   <  2:857038/150‑1 (MQ=255)
                                                                                                                               ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                   <  2:1103366/150‑1 (MQ=255)
                                                                                                                               ccTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                   <  1:4252228/150‑1 (MQ=255)
                                                                                                                                 tGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGc                 >  2:1922438/1‑150 (MQ=255)
                                                                                                                                  gCCAGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGcc                <  2:3486622/150‑1 (MQ=255)
                                                                                                                                   ccAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCg               >  2:711445/1‑150 (MQ=255)
                                                                                                                                     aGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGtt             <  1:2761000/150‑1 (MQ=255)
                                                                                                                                     aGTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGtt             <  2:1719695/150‑1 (MQ=255)
                                                                                                                                      gTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc            <  1:438420/150‑1 (MQ=255)
                                                                                                                                      gTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc            <  1:4137005/150‑1 (MQ=255)
                                                                                                                                      gTTCGTTGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc            >  1:65186/1‑150 (MQ=255)
                                                                                                                                        tCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgc          >  2:2172308/1‑150 (MQ=255)
                                                                                                                                        tCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgc          <  2:4368025/150‑1 (MQ=255)
                                                                                                                                        tCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgc          >  2:2257781/1‑150 (MQ=255)
                                                                                                                                         ctttGGCTGGAGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgcc         >  2:4250377/3‑150 (MQ=255)
                                                                                                                                              gctggcGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCa    <  2:2307852/150‑1 (MQ=255)
                                                                                                                                                tggaGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGa  <  1:2685045/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGA  >  CP009273/4274601‑4274894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑