Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,373,880 G→T 14.9% M75I (ATG→ATT)  yjeM → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,373,8800GT14.9% 965.7 / 101.1 295M75I (ATG→ATT) yjeMputative transporter
Reads supporting (aligned to +/- strand):  ref base G (140/111);  new base T (18/26);  total (158/137)
Fisher's exact test for biased strand distribution p-value = 7.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 295 total aligned reads displayed.

ATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGT  >  CP009273/4373732‑4374027
                                                                                                                                                    |                                                                                                                                                   
aTAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGa                                                                                                                                                    <  1:1726905/150‑1 (MQ=255)
     ccATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGaataat                                                                                                                                               >  2:2260609/1‑150 (MQ=255)
      cATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAataata                                                                                                                                              <  1:1087939/150‑1 (MQ=255)
       aTCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAg                                                                                                                                             >  1:3162297/1‑150 (MQ=255)
       aTCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCAGTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAACTGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAg                                                                                                                                             >  2:3983061/1‑150 (MQ=255)
          ggCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCTTGGATTAATAATAgtgt                                                                                                                                          >  1:3201018/1‑150 (MQ=255)
           gCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTc                                                                                                                                         <  1:606096/150‑1 (MQ=255)
              tattaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGa                                                                                                                                      <  1:534451/150‑1 (MQ=255)
              tattaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGa                                                                                                                                      <  1:2009405/150‑1 (MQ=255)
                ttaCTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGAcc                                                                                                                                    >  2:1721990/1‑150 (MQ=255)
                   cTTACTGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACg                                                                                                                                 <  2:303581/150‑1 (MQ=255)
                   cTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACg                                                                                                                                 <  2:3897038/150‑1 (MQ=255)
                   cTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACg                                                                                                                                 >  1:4574131/1‑150 (MQ=255)
                           gTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCt                                                                                                                         <  2:3288578/150‑1 (MQ=255)
                           gTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCt                                                                                                                         <  2:1962493/150‑1 (MQ=255)
                               taGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCAt                                                                                                                     <  2:2429530/150‑1 (MQ=255)
                               taGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCAt                                                                                                                     >  1:4420316/1‑150 (MQ=255)
                               taGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCAt                                                                                                                     >  1:3028052/1‑150 (MQ=255)
                                aGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCAtt                                                                                                                    >  1:3949691/1‑150 (MQ=255)
                                    cGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTa                                                                                                                >  2:2111699/1‑150 (MQ=255)
                                    cGAGTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTa                                                                                                                <  1:4384476/150‑1 (MQ=255)
                                     gATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTAc                                                                                                               >  2:4357898/1‑150 (MQ=255)
                                        tCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGtt                                                                                                            <  1:2839441/150‑1 (MQ=255)
                                        tCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGtt                                                                                                            <  1:3808728/150‑1 (MQ=255)
                                          ccTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTa                                                                                                          <  1:3609392/150‑1 (MQ=255)
                                          ccTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTa                                                                                                          >  1:1171286/1‑150 (MQ=255)
                                           cTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGATCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTAt                                                                                                         >  1:808602/1‑150 (MQ=255)
                                           cTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTAt                                                                                                         >  2:1700920/1‑150 (MQ=255)
                                            ttttATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTAtg                                                                                                        <  2:4082586/150‑1 (MQ=255)
                                                  atatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGttt                                                                                                  >  1:948703/1‑150 (MQ=255)
                                                  atatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGttt                                                                                                  >  2:2212324/1‑150 (MQ=255)
                                                  atatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGttt                                                                                                  <  2:3773471/150‑1 (MQ=255)
                                                   tatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGtttt                                                                                                 >  2:4214932/1‑150 (MQ=255)
                                                   tatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGtttt                                                                                                 >  2:940624/1‑150 (MQ=255)
                                                   tatTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGtttt                                                                                                 >  1:1362989/1‑150 (MQ=255)
                                                     ttttCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTcc                                                                                               <  1:1970020/150‑1 (MQ=255)
                                                      tttCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCct                                                                                              <  1:4453125/150‑1 (MQ=255)
                                                      tttCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCct                                                                                              <  2:306888/150‑1 (MQ=255)
                                                         cTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCtt                                                                                           >  2:46229/1‑150 (MQ=255)
                                                          tGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTta                                                                                          <  2:1264773/150‑1 (MQ=255)
                                                          tGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTta                                                                                          <  1:2962905/150‑1 (MQ=255)
                                                           gCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTtat                                                                                         <  1:2254210/150‑1 (MQ=255)
                                                             aTTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTCTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTatat                                                                                       >  1:3500663/1‑150 (MQ=255)
                                                               tGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATatca                                                                                     >  1:248074/1‑150 (MQ=255)
                                                               tGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATatca                                                                                     >  1:2564607/1‑150 (MQ=255)
                                                               tGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATatca                                                                                     <  2:2268005/150‑1 (MQ=255)
                                                                 ttATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATatcatc                                                                                   >  2:3261583/1‑150 (MQ=255)
                                                                  tATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCt                                                                                  >  2:4369967/1‑150 (MQ=255)
                                                                    ttCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTgg                                                                                >  2:3156662/1‑150 (MQ=255)
                                                                       tttATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATg                                                                             <  1:3318822/150‑1 (MQ=255)
                                                                       tttATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGGTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATg                                                                             >  2:4168775/1‑150 (MQ=255)
                                                                        ttATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATgg                                                                            >  2:1995371/1‑150 (MQ=255)
                                                                             ccATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGt                                                                       >  2:2810741/1‑150 (MQ=255)
                                                                              cATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTa                                                                      >  2:1017557/1‑150 (MQ=255)
                                                                               aTTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTAc                                                                     >  2:1934997/1‑150 (MQ=255)
                                                                                  cGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTc                                                                  >  2:3443572/1‑150 (MQ=255)
                                                                                  cGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTc                                                                  >  2:636637/1‑150 (MQ=255)
                                                                                   gCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTcc                                                                 <  1:2643249/150‑1 (MQ=255)
                                                                                        aaTGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGaa                                                            <  2:3625710/150‑1 (MQ=255)
                                                                                         atgatgGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGaaa                                                           >  2:874273/1‑150 (MQ=255)
                                                                                             tgGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGttt                                                       <  2:2291104/150‑1 (MQ=255)
                                                                                                 tGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGt                                                   >  1:1246629/1‑150 (MQ=255)
                                                                                                  gAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTa                                                  >  1:2316816/1‑150 (MQ=255)
                                                                                                     aTGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTGTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCg                                               >  2:3558145/1‑150 (MQ=255)
                                                                                                        ggAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTtc                                            <  1:4499005/150‑1 (MQ=255)
                                                                                                           gctgctTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCa                                         <  1:3559122/150‑1 (MQ=255)
                                                                                                            ctgctTATCGCAAAGAAGAAGGCGGTATCTATTCTTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCaa                                        <  2:3201018/150‑1 (MQ=255)
                                                                                                             tgctTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCTAAAGTTTGGGTACCGTTCTCAAc                                       <  1:1196349/150‑1 (MQ=255)
                                                                                                               ctTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACAt                                     >  2:2945317/1‑150 (MQ=255)
                                                                                                               ctTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACAt                                     >  2:1587021/1‑150 (MQ=255)
                                                                                                                 tATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTc                                   <  2:4450578/150‑1 (MQ=255)
                                                                                                                 tATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTc                                   <  2:2564607/150‑1 (MQ=255)
                                                                                                                   tCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCct                                 <  1:1499559/150‑1 (MQ=255)
                                                                                                                      cAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTa                              <  1:3499542/150‑1 (MQ=255)
                                                                                                                        aagaagaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATg                            >  2:2926303/1‑150 (MQ=255)
                                                                                                                        aagaagaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCGTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATg                            >  2:1789952/1‑150 (MQ=255)
                                                                                                                         agaagaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATgg                           >  2:584779/1‑150 (MQ=255)
                                                                                                                         agaagaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATgg                           <  2:3842507/150‑1 (MQ=255)
                                                                                                                           aagaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTa                         >  1:21442/1‑150 (MQ=255)
                                                                                                                             gaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGc                       >  1:2051205/1‑150 (MQ=255)
                                                                                                                             gaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGc                       <  1:2925007/150‑1 (MQ=255)
                                                                                                                             gaagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTGTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGc                       >  2:446925/1‑150 (MQ=255)
                                                                                                                              aagGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCg                      >  1:1849347/1‑150 (MQ=255)
                                                                                                                                    gTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGa                <  1:4555517/150‑1 (MQ=255)
                                                                                                                                    gTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGa                >  1:3178619/1‑150 (MQ=255)
                                                                                                                                       tCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGAccc             <  2:494343/150‑1 (MQ=255)
                                                                                                                                        cTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCa            <  2:207632/150‑1 (MQ=255)
                                                                                                                                         tATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAg           >  1:2198722/1‑150 (MQ=255)
                                                                                                                                         tATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAg           >  1:4080774/1‑150 (MQ=255)
                                                                                                                                          aTTCCTGGATTAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGAGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCAATTGTAGCGACATGACCCAGc          >  2:1157260/1‑150 (MQ=255)
                                                                                                                                          aTTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGc          >  1:2375270/1‑150 (MQ=255)
                                                                                                                                          aTTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGc          >  2:1472685/1‑150 (MQ=255)
                                                                                                                                          aTTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGc          >  2:14555/1‑150 (MQ=255)
                                                                                                                                          aTTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGc          <  1:3388900/150‑1 (MQ=255)
                                                                                                                                          aTTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGc          >  1:1686490/1‑150 (MQ=255)
                                                                                                                                           ttCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCa         >  1:3832601/1‑150 (MQ=255)
                                                                                                                                            tCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCAc        >  1:2340109/1‑150 (MQ=255)
                                                                                                                                             ccTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACt       >  2:530407/1‑150 (MQ=255)
                                                                                                                                                 gATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCg   <  1:2735795/150‑1 (MQ=255)
                                                                                                                                                  aTGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGt  >  1:426477/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATAGCCCATCGGCTTATTACTTAATGGGTTATAGTGCGATTCCCTTTTATATATTTTCTGCATTGTTATTCTTTATTCCATTCGCCTTAATGATGGCTGAAATGGGAGCTGCTTATCGCAAAGAAGAAGGCGGTATCTATTCCTGGATGAATAATAGTGTCGGACCACGTTTTGCCTTCATTGGTACGTTTATGTGGTTTTCCTCTTATATCATCTGGATGGTGAGTACCTCCGCGAAAGTTTGGGTACCGTTCTCAACATTCCTCTATGGTAGCGACATGACCCAGCACTGGCGT  >  CP009273/4373732‑4374027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑