BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,407,017	C→T	60.1%	I83I (ATC→ATT)	yjfP →	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,407,017	0	C	T	60.1%	209.2 / inf	315	I83I (ATC→ATT)	yjfP	acyl CoA esterase
Reads supporting (aligned to +/- strand): ref base C (50/75); new base T (81/108); total (131/184)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 318 total aligned reads displayed.

CACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAG  >  CP009273/4406868‑4407163

cACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                                                                                                                                    >  2:187104/1‑150 (MQ=255)

cACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                                                                                                                                    <  1:2362931/150‑1 (MQ=255)

  cGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCtt                                                                                                                                                  <  1:3444687/150‑1 (MQ=255)

   ggCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTg                                                                                                                                                 >  2:699984/1‑150 (MQ=255)

   ggCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTg                                                                                                                                                 >  1:2234308/1‑150 (MQ=255)

    gCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGc                                                                                                                                                >  1:731871/1‑150 (MQ=255)

       ttACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTAc                                                                                                                                             <  1:3349433/150‑1 (MQ=255)

       ttACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTAc                                                                                                                                             >  1:367765/1‑150 (MQ=255)

        tACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACa                                                                                                                                            <  2:3238572/150‑1 (MQ=255)

         aCTTCATCCAGTCTGGTGTATAGCTATTTTGCCGATGCGCTGGCGCAGGCTTGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACaa                                                                                                                                           >  1:2997211/1‑150 (MQ=255)

            tCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGt                                                                                                                                        <  1:4085010/150‑1 (MQ=255)

               tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATg                                                                                                                                     <  1:592697/150‑1 (MQ=255)

               tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATg                                                                                                                                     <  1:4210036/150‑1 (MQ=255)

                  aGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAg                                                                                                                                  >  1:863454/1‑150 (MQ=255)

                     cTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGaa                                                                                                                               <  1:3173186/150‑1 (MQ=255)

                        gtgtATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTc                                                                                                                            <  2:1247557/150‑1 (MQ=255)

                        gtgtATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTc                                                                                                                            <  2:1984488/150‑1 (MQ=255)

                         tgtATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCa                                                                                                                           <  1:1209918/150‑1 (MQ=255)

                          gtATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCac                                                                                                                          >  2:2398699/1‑150 (MQ=255)

                           tataGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCact                                                                                                                         <  1:186082/150‑1 (MQ=255)

                              aGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCactact                                                                                                                      <  1:2139496/150‑1 (MQ=255)

                              aGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCAGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCactact                                                                                                                      <  2:4063985/150‑1 (MQ=255)

                               gCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACtt                                                                                                                     <  2:355980/150‑1 (MQ=255)

                                cTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACttt                                                                                                                    >  2:782570/1‑150 (MQ=255)

                                cTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACttt                                                                                                                    <  2:1128839/150‑1 (MQ=255)

                                 tATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTa                                                                                                                   <  2:1197355/150‑1 (MQ=255)

                                  aTTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTAc                                                                                                                  <  2:3786525/150‑1 (MQ=255)

                                          gTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCa                                                                                                          <  2:4176802/150‑1 (MQ=255)

                                          gTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCa                                                                                                          <  2:2783160/150‑1 (MQ=255)

                                          gTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCa                                                                                                          >  2:4316855/1‑150 (MQ=255)

                                          gTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCa                                                                                                          >  1:1056194/1‑150 (MQ=255)

                                           ttGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCaa                                                                                                         <  1:4333879/150‑1 (MQ=255)

                                            tGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAAt                                                                                                        >  1:1527297/1‑150 (MQ=255)

                                             gcgcTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCATTTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATa                                                                                                       <  2:2332183/150‑1 (MQ=255)

                                                cTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGcc                                                                                                    <  1:4171201/150‑1 (MQ=255)

                                                   gcgcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaa                                                                                                 >  2:2442112/1‑150 (MQ=255)

                                                    cgcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaag                                                                                                <  1:1784234/150‑1 (MQ=255)

                                                    cgcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaag                                                                                                >  1:1484661/1‑150 (MQ=255)

                                                    cgcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaag                                                                                                <  2:202632/150‑1 (MQ=255)

                                                    cgcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaag                                                                                                <  1:940920/150‑1 (MQ=255)

                                                      cAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCgaagaa                                                                                              <  2:2940203/150‑1 (MQ=255)

                                                          cTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACt                                                                                          >  2:3514777/1‑150 (MQ=255)

                                                          cTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACt                                                                                          <  2:3458192/150‑1 (MQ=255)

                                                               ttGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgctg                                                                                     >  1:1016013/1‑150 (MQ=255)

                                                               ttGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgctg                                                                                     <  1:1239105/150‑1 (MQ=255)

                                                                tGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgctgc                                                                                    <  1:3256525/150‑1 (MQ=255)

                                                                   gggTGATCATGCCGAATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtg                                                                                 <  1:2335249/150‑1 (MQ=255)

                                                                     gTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtgat                                                                               >  1:2085768/1‑150 (MQ=255)

                                                                      tGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtgatg                                                                              <  2:574987/150‑1 (MQ=255)

                                                                        aTCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGAc                                                                            <  2:2102867/150‑1 (MQ=255)

                                                                        aTCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGAc                                                                            >  1:1597756/1‑150 (MQ=255)

                                                                         tCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCACTAGCCGAAGAAAACTGGCTGCTTGATGAcc                                                                           >  2:2141831/1‑150 (MQ=255)

                                                                          cATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCg                                                                          >  1:1534456/1‑150 (MQ=255)

                                                                             gCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCt                                                                       <  2:1819518/150‑1 (MQ=255)

                                                                               cGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTgg                                                                     <  1:3759389/150‑1 (MQ=255)

                                                                                   tGCGCCCGTTCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACATAGTATGGAGGTATTCACTACTTTACGTGCGGCTATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGt                                                                 <  1:1961974/150‑1 (MQ=255)

                                                                                    gcgcCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTc                                                                <  2:2978503/150‑1 (MQ=255)

                                                                                       cccGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGt                                                             <  1:1647016/150‑1 (MQ=255)

                                                                                        ccGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTg                                                            <  2:1478959/150‑1 (MQ=255)

                                                                                             cACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGcgcg                                                       <  1:4430251/150‑1 (MQ=255)

                                                                                             cACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGcgcg                                                       <  1:2749814/150‑1 (MQ=255)

                                                                                                ggTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCg                                                    <  1:1434812/150‑1 (MQ=255)

                                                                                                ggTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCg                                                    >  2:3947155/1‑150 (MQ=255)

                                                                                                  tAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGAt                                                  <  1:488626/150‑1 (MQ=255)

                                                                                                   aGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATg                                                 >  2:1373333/1‑150 (MQ=255)

                                                                                                      cGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgc                                              >  2:881231/1‑150 (MQ=255)

                                                                                                      cGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgc                                              <  2:1011527/150‑1 (MQ=255)

                                                                                                      cGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCGTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgc                                              <  2:386427/150‑1 (MQ=255)

                                                                                                       gTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgcg                                             >  2:911380/1‑150 (MQ=255)

                                                                                                        ttttAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgcgc                                            <  1:839061/150‑1 (MQ=255)

                                                                                                           tAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGAt                                         <  1:4619941/150‑1 (MQ=255)

                                                                                                            aGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATg                                        >  2:2750470/1‑150 (MQ=255)

                                                                                                            aGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATg                                        <  2:1265017/150‑1 (MQ=255)

                                                                                                               ggtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACg                                     <  1:4450231/150‑1 (MQ=255)

                                                                                                                    cGCAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACt                                >  2:1667699/1‑150 (MQ=255)

                                                                                                                      cAGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTgg                              >  1:4517906/1‑150 (MQ=255)

                                                                                                                       aGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTggg                             >  1:26684/1‑150 (MQ=255)

                                                                                                                       aGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTggg                             >  2:2530895/1‑150 (MQ=255)

                                                                                                                       aGCGCGGCGGTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTggg                             >  2:2814352/1‑150 (MQ=255)

                                                                                                                          gcggcgGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGAt                          >  2:2218716/1‑150 (MQ=255)

                                                                                                                           cggcggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGAtt                         <  2:2234308/150‑1 (MQ=255)

                                                                                                                             gcggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTAc                       >  1:3328413/1‑150 (MQ=255)

                                                                                                                              cggTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTAcc                      <  2:4642220/150‑1 (MQ=255)

                                                                                                                               ggTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCg                     >  1:1683103/1‑150 (MQ=255)

                                                                                                                               ggTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCg                     <  2:3250623/150‑1 (MQ=255)

                                                                                                                                gTTAAATCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGc                    <  2:399157/150‑1 (MQ=255)

                                                                                                                                  tAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTc                  >  1:1379880/1‑150 (MQ=255)

                                                                                                                                     aTCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGTTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGcc               >  1:3443874/1‑150 (MQ=255)

                                                                                                                                     aTCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGcc               >  1:3895976/1‑150 (MQ=255)

                                                                                                                                     aTCAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGcc               <  1:2523649/150‑1 (MQ=255)

                                                                                                                                       cAATTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAc             <  1:3941252/150‑1 (MQ=255)

                                                                                                                                        aaTTCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAcc            >  1:3827858/1‑150 (MQ=255)

                                                                                                                                           tCTGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCa         <  1:4018820/150‑1 (MQ=255)

                                                                                                                                             tGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACg       >  2:1781315/1‑150 (MQ=255)

                                                                                                                                             tGGCAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACg       >  2:2830548/1‑150 (MQ=255)

                                                                                                                                               gCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGt     <  1:1874449/150‑1 (MQ=255)

                                                                                                                                                cAAATTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTg    >  1:3502577/1‑150 (MQ=255)

                                                                                                                                                 aaaTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTga   <  2:1900512/150‑1 (MQ=255)

                                                                                                                                                  aaTTTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag  <  1:685797/150‑1 (MQ=255)

                                                                                                                                                  aaTCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTgag  <  2:1527297/150‑1 (MQ=255)

CACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAG  >  CP009273/4406868‑4407163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`