Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,254 T→A 100% V271V (GTT→GTA)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,2540TA98.8% 1146.8 / 2.1 329V271V (GTT→GTA) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (166/159);  total (169/160)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 329 total aligned reads displayed.

CGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTG  >  CP009273/4458107‑4458398
                                                                                                                                                   |                                                                                                                                                
cGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAg                                                                                                                                                <  1:4035673/150‑1 (MQ=255)
cGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAg                                                                                                                                                >  2:3587473/1‑150 (MQ=255)
 gCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGTGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGt                                                                                                                                               <  2:899717/150‑1 (MQ=255)
     ccACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGc                                                                                                                                           >  1:3331790/1‑150 (MQ=255)
      cACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCa                                                                                                                                          >  1:189418/1‑150 (MQ=255)
       aCTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAg                                                                                                                                         >  2:3903425/1‑150 (MQ=255)
          cGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGaa                                                                                                                                      >  1:427090/1‑150 (MQ=255)
             cAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGc                                                                                                                                   >  2:1443520/1‑150 (MQ=255)
             cAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGc                                                                                                                                   >  1:616413/1‑150 (MQ=255)
             cAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTGTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCCATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGc                                                                                                                                   >  1:2785401/1‑150 (MQ=255)
              aGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCg                                                                                                                                  >  1:3967586/1‑150 (MQ=255)
                 cGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGc                                                                                                                               <  1:556077/150‑1 (MQ=255)
                  ggAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGcc                                                                                                                              <  2:2988367/150‑1 (MQ=255)
                   gAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCg                                                                                                                             <  2:2834106/150‑1 (MQ=255)
                     gCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGaa                                                                                                                           <  2:1837510/150‑1 (MQ=255)
                     gCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGaa                                                                                                                           >  2:4443726/1‑150 (MQ=255)
                      cacaGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAAt                                                                                                                          <  1:786702/150‑1 (MQ=255)
                      cacaGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAAt                                                                                                                          <  2:728490/150‑1 (MQ=255)
                       acaGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATg                                                                                                                         >  1:504615/1‑150 (MQ=255)
                        caGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGc                                                                                                                        >  2:2567654/1‑150 (MQ=255)
                           cAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCtt                                                                                                                     >  2:2773305/1‑150 (MQ=255)
                           cAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCtt                                                                                                                     <  2:1323909/150‑1 (MQ=255)
                             aTCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTc                                                                                                                   >  2:1879167/1‑150 (MQ=255)
                              tCCGCTGGTGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCa                                                                                                                  <  1:1680527/150‑1 (MQ=255)
                               ccGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAg                                                                                                                 <  2:3454539/150‑1 (MQ=255)
                               ccGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAg                                                                                                                 >  1:4401192/1‑150 (MQ=255)
                               ccGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAg                                                                                                                 <  2:1227769/150‑1 (MQ=255)
                                  cTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCaa                                                                                                              >  1:267087/1‑150 (MQ=255)
                                  cTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCaa                                                                                                              >  2:4253723/1‑150 (MQ=255)
                                       gTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAt                                                                                                         <  2:4032143/150‑1 (MQ=255)
                                       gTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAt                                                                                                         <  1:461924/150‑1 (MQ=255)
                                        tGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATc                                                                                                        >  2:56812/1‑150 (MQ=255)
                                        tGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATAGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATc                                                                                                        >  2:3117530/1‑150 (MQ=255)
                                         gCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCa                                                                                                       <  2:3130453/150‑1 (MQ=255)
                                         gCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCa                                                                                                       <  1:4160616/150‑1 (MQ=255)
                                          cGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAg                                                                                                      <  1:643845/150‑1 (MQ=255)
                                              acGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCcgc                                                                                                  <  1:2912026/150‑1 (MQ=255)
                                                 cTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTc                                                                                               <  2:3740040/150‑1 (MQ=255)
                                                  tgtgTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCa                                                                                              <  2:4318607/150‑1 (MQ=255)
                                                   gtgtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAg                                                                                             <  2:4134868/150‑1 (MQ=255)
                                                    tgtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGt                                                                                            >  2:2725576/1‑150 (MQ=255)
                                                     gtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTa                                                                                           >  1:3163824/1‑150 (MQ=255)
                                                     gtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTa                                                                                           >  2:2673989/1‑150 (MQ=255)
                                                      ttttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                          <  2:380196/150‑1 (MQ=255)
                                                         tATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgct                                                                                       <  2:3942390/150‑1 (MQ=255)
                                                            gggCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATgctgct                                                                                    >  1:1431413/1‑150 (MQ=255)
                                                             ggCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGctgctg                                                                                   <  1:2226389/150‑1 (MQ=255)
                                                                 ccaccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTc                                                                               <  1:859442/150‑1 (MQ=255)
                                                                  caccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCCAGACCTGGGTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGACAGTAGGCTGCTGATTCg                                                                              >  2:1969228/1‑150 (MQ=255)
                                                                  caccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCg                                                                              >  1:4470240/1‑150 (MQ=255)
                                                                   accGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGc                                                                             >  1:2717661/1‑150 (MQ=255)
                                                                      gtggtgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCttt                                                                          >  1:229529/1‑150 (MQ=255)
                                                                      gtggtgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCttt                                                                          <  2:3967586/150‑1 (MQ=255)
                                                                          tgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGc                                                                      >  1:4212503/1‑150 (MQ=255)
                                                                            aGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTg                                                                    <  2:2410846/150‑1 (MQ=255)
                                                                             gCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTgg                                                                   <  2:3342935/150‑1 (MQ=255)
                                                                               ggcacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTg                                                                 >  2:2256654/1‑150 (MQ=255)
                                                                               ggcacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTg                                                                 <  2:3276075/150‑1 (MQ=255)
                                                                                gcacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGa                                                                <  1:2457220/150‑1 (MQ=255)
                                                                                   cggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGTCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATgg                                                             <  1:1608308/150‑1 (MQ=255)
                                                                                   cggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATgg                                                             >  1:3771886/1‑150 (MQ=255)
                                                                                    ggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGc                                                            <  2:4119205/150‑1 (MQ=255)
                                                                                    ggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGc                                                            >  2:562972/1‑150 (MQ=255)
                                                                                        caAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCg                                                        >  2:3695545/1‑150 (MQ=255)
                                                                                        caAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCg                                                        <  1:847152/150‑1 (MQ=255)
                                                                                             aaTGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCATCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtggt                                                   <  1:2133774/150‑1 (MQ=255)
                                                                                                ggTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCt                                                <  1:1003003/150‑1 (MQ=255)
                                                                                                ggTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCt                                                <  1:3881475/150‑1 (MQ=255)
                                                                                                   gATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGtt                                             <  1:3265860/150‑1 (MQ=255)
                                                                                                   gATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGtt                                             <  1:1328518/150‑1 (MQ=255)
                                                                                                   gATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGtt                                             >  1:2478575/1‑150 (MQ=255)
                                                                                                   gATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGtt                                             >  1:1138189/1‑150 (MQ=255)
                                                                                                     ttGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCGGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTaa                                           >  1:3110951/1‑150 (MQ=255)
                                                                                                      tGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAAt                                          >  1:1243712/1‑150 (MQ=255)
                                                                                                       gCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATc                                         >  2:4640508/1‑150 (MQ=255)
                                                                                                       gCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATc                                         >  2:4198958/1‑150 (MQ=255)
                                                                                                        cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCa                                        >  2:3883205/1‑150 (MQ=255)
                                                                                                        cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCa                                        >  2:2464475/1‑150 (MQ=255)
                                                                                                        cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGGAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCa                                        >  2:3528954/1‑150 (MQ=255)
                                                                                                          aCAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAAt                                      >  2:2074765/1‑150 (MQ=255)
                                                                                                          aCAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAAt                                      <  1:3485935/150‑1 (MQ=255)
                                                                                                           cAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATg                                     >  1:193250/1‑150 (MQ=255)
                                                                                                                gCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTac                                >  1:1600748/1‑150 (MQ=255)
                                                                                                                  cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTacac                              <  2:193985/150‑1 (MQ=255)
                                                                                                                  cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTacac                              >  2:1009677/1‑150 (MQ=255)
                                                                                                                   aaTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACAcc                             <  2:1680197/150‑1 (MQ=255)
                                                                                                                        cTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAgg                        >  1:2047278/1‑150 (MQ=255)
                                                                                                                         tGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGc                       <  1:3796597/150‑1 (MQ=255)
                                                                                                                          ggTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCg                      >  1:3661745/1‑150 (MQ=255)
                                                                                                                               ggTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtgg                 <  1:297677/150‑1 (MQ=255)
                                                                                                                                   aaCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTggg             >  1:2281016/1‑150 (MQ=255)
                                                                                                                                   aaCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTggg             >  2:2178881/1‑150 (MQ=255)
                                                                                                                                    aCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGa            >  1:1454001/1‑150 (MQ=255)
                                                                                                                                      tGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAg          >  1:1812428/1‑150 (MQ=255)
                                                                                                                                       ggCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgc         <  2:1087242/150‑1 (MQ=255)
                                                                                                                                          ggggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcggc      <  1:2746448/150‑1 (MQ=255)
                                                                                                                                           gggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcggcg     >  1:3728899/1‑150 (MQ=255)
                                                                                                                                           gggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcggcg     >  1:2174875/1‑150 (MQ=255)
                                                                                                                                             gCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCt   <  1:1480965/150‑1 (MQ=255)
                                                                                                                                              cGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTg  <  1:3587647/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTG  >  CP009273/4458107‑4458398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑