Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,470,017 G→A 100% M157I (ATG→ATA)  yjgN → DUF898 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,470,0170GA96.4% 960.8 / 17.4 279M157I (ATG→ATA) yjgNDUF898 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (3/7);  new base A (150/119);  total (153/126)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 282 total aligned reads displayed.

GTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTC  >  CP009273/4469871‑4470162
                                                                                                                                                  |                                                                                                                                                 
gTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATaggg                                                                                                                                                <  2:1358265/150‑1 (MQ=255)
 ttttGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGa                                                                                                                                               >  2:1696232/1‑150 (MQ=255)
    tGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGAGTg                                                                                                                                            >  2:4188351/1‑150 (MQ=255)
    tGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTg                                                                                                                                            <  2:217905/150‑1 (MQ=255)
      cTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGtt                                                                                                                                          <  1:1017993/150‑1 (MQ=255)
        tataTTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGtttt                                                                                                                                        >  1:1048773/1‑150 (MQ=255)
            tttATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCttt                                                                                                                                    >  1:3599668/1‑150 (MQ=255)
            tttATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTAttt                                                                                                                                    <  1:3165488/150‑1 (MQ=255)
             ttATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTa                                                                                                                                   <  2:4481573/150‑1 (MQ=255)
              tATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTAt                                                                                                                                  >  1:2884529/1‑150 (MQ=255)
                tGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTAtct                                                                                                                                >  2:1984320/1‑150 (MQ=255)
                  gcagcaAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCg                                                                                                                              <  2:3086472/150‑1 (MQ=255)
                  gcagcaAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATGTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCg                                                                                                                              >  1:2068144/1‑150 (MQ=255)
                     gcaAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACa                                                                                                                           <  2:1343244/150‑1 (MQ=255)
                       aaaaGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACaaa                                                                                                                         <  1:1690209/150‑1 (MQ=255)
                       aaaaGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACaaa                                                                                                                         >  1:3024653/1‑150 (MQ=255)
                       aaaaGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACaaa                                                                                                                         <  1:2413272/150‑1 (MQ=255)
                        aaaGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAg                                                                                                                        <  2:3237939/150‑1 (MQ=255)
                         aaGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGa                                                                                                                       >  2:2403719/1‑150 (MQ=255)
                          aGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGAt                                                                                                                      <  2:3014722/150‑1 (MQ=255)
                             aCTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCt                                                                                                                   >  1:3725502/1‑150 (MQ=255)
                             aCTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCt                                                                                                                   >  1:3428106/1‑150 (MQ=255)
                              cTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTa                                                                                                                  >  2:1392176/1‑150 (MQ=255)
                                gCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTAcc                                                                                                                <  2:2298707/150‑1 (MQ=255)
                                  gTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTg                                                                                                              >  1:2598490/1‑150 (MQ=255)
                                  gTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTg                                                                                                              <  2:1621557/150‑1 (MQ=255)
                                  gTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTg                                                                                                              >  2:3028778/1‑150 (MQ=255)
                                   tcatcaGGCCTTAGTGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATGTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGc                                                                                                             >  2:817998/1‑150 (MQ=255)
                                     atcaGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCa                                                                                                           <  1:1106646/150‑1 (MQ=255)
                                         ggCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAg                                                                                                       >  2:4314835/1‑150 (MQ=255)
                                          gCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGt                                                                                                      >  2:4635402/1‑150 (MQ=255)
                                           ccTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGtt                                                                                                     >  1:3628715/1‑150 (MQ=255)
                                                        tatcAACTGCTAAAGATTTAGTATTAATTGCTCTAAGAAAGGGTTATGTTGGGTGACCAATTTCTTGCCGATTTTAATGGCCATTTGCATATCGAATGTTTACTATATCTCGAAAAAGATCATAACTGCCAATAGTTCAAGTAGTTttat                                                                                        >  2:3686720/3‑150 (MQ=255)
                                                         ctcAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGttatt                                                                                       <  2:1406463/150‑1 (MQ=255)
                                                          tcAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGttatta                                                                                      >  2:4229995/1‑150 (MQ=255)
                                                          tcAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGttatta                                                                                      >  2:1082930/1‑150 (MQ=255)
                                                             aCGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTatat                                                                                   <  2:52525/150‑1 (MQ=255)
                                                              cGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATc                                                                                  >  2:2744919/1‑150 (MQ=255)
                                                               ggCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATcc                                                                                 <  2:3585589/150‑1 (MQ=255)
                                                               ggCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATcc                                                                                 >  2:1165372/1‑150 (MQ=255)
                                                                 cGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCAt                                                                               >  1:1306801/1‑150 (MQ=255)
                                                                  gTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATg                                                                              >  2:1712256/1‑150 (MQ=255)
                                                                    aaGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGt                                                                            <  2:2683251/150‑1 (MQ=255)
                                                                         atAGTTTTAATTGCTCTATGAAAGGGTTCTGGAGGGTGACCTTTTTCTTGACGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCACGACAAAGATGCTACCAGACAATAGTTCAAGTAGAGTTATTATATAAATGGTTCTGa                                                                       >  2:1871105/2‑150 (MQ=255)
                                                                          tAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGAt                                                                      <  1:2363952/150‑1 (MQ=255)
                                                                          tAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAAAGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGAt                                                                      <  1:3304997/150‑1 (MQ=255)
                                                                             ttttAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGc                                                                   >  1:2784927/1‑150 (MQ=255)
                                                                              tttAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCa                                                                  >  2:1193862/1‑150 (MQ=255)
                                                                              tttAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCa                                                                  <  2:1466744/150‑1 (MQ=255)
                                                                                 aaTTGCTCTATTAAAGTGTTCTGGTGGGTGACCTTTTTCTTGACGATTTTCATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATa                                                               <  1:2315657/150‑1 (MQ=255)
                                                                                  aTTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAg                                                              >  2:713177/1‑150 (MQ=255)
                                                                                   ttGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGt                                                             >  1:1962060/1‑150 (MQ=255)
                                                                                    tGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGtt                                                            >  1:473672/1‑150 (MQ=255)
                                                                                     gCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTg                                                           >  1:148968/1‑150 (MQ=255)
                                                                                        ctATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTa                                                        >  2:1004084/1‑150 (MQ=255)
                                                                                         tATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTAt                                                       >  1:3924478/1‑150 (MQ=255)
                                                                                         tATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTAt                                                       >  1:2181338/1‑150 (MQ=255)
                                                                                          aTGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTAtt                                                      >  2:847190/1‑150 (MQ=255)
                                                                                          aTGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTAtt                                                      <  1:44165/150‑1 (MQ=255)
                                                                                          aTGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTAtt                                                      >  1:2857064/1‑150 (MQ=255)
                                                                                           tGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTg                                                     >  1:4119881/1‑150 (MQ=255)
                                                                                            gAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATGTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGt                                                    >  1:1254746/1‑150 (MQ=255)
                                                                                             aaaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGtt                                                   <  2:4426335/150‑1 (MQ=255)
                                                                                             aaaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGtt                                                   <  2:3424834/150‑1 (MQ=255)
                                                                                             aaaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGtt                                                   >  2:3954455/1‑150 (MQ=255)
                                                                                             aaaGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGtt                                                   >  1:3476160/1‑150 (MQ=255)
                                                                                                gggTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTcc                                                <  1:4460885/150‑1 (MQ=255)
                                                                                                gggTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTcc                                                >  2:1027612/1‑150 (MQ=255)
                                                                                                 ggTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCa                                               >  1:2019562/1‑150 (MQ=255)
                                                                                                  gTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCAt                                              <  2:2967155/150‑1 (MQ=255)
                                                                                                   ttCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCAtt                                             <  1:2216521/150‑1 (MQ=255)
                                                                                                    tCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTg                                            >  1:3976400/1‑150 (MQ=255)
                                                                                                    tCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTg                                            <  1:3103468/150‑1 (MQ=255)
                                                                                                         tgggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTAtt                                       >  2:429530/1‑150 (MQ=255)
                                                                                                          gggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTCTCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTAttt                                      <  1:854703/150‑1 (MQ=255)
                                                                                                           ggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTAtttt                                     <  1:4052819/150‑1 (MQ=255)
                                                                                                           ggtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTAtttt                                     <  1:1548515/150‑1 (MQ=255)
                                                                                                            gtgACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTAttttt                                    >  2:1484939/1‑150 (MQ=255)
                                                                                                                ccTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATg                                >  2:2871426/1‑150 (MQ=255)
                                                                                                                ccTTTTTCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTACATTGGTATTTTTAATg                                >  2:2721899/1‑150 (MQ=255)
                                                                                                                  tttttCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGt                              <  2:1242562/150‑1 (MQ=255)
                                                                                                                   ttttCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTa                             <  1:2375998/150‑1 (MQ=255)
                                                                                                                      tCTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACtt                          <  2:1755348/150‑1 (MQ=255)
                                                                                                                       cTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACttt                         <  2:2800546/150‑1 (MQ=255)
                                                                                                                       cTTGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACttt                         <  2:2003188/150‑1 (MQ=255)
                                                                                                                         tGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTtat                       <  2:1155728/150‑1 (MQ=255)
                                                                                                                         tGCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTtat                       <  1:4181320/150‑1 (MQ=255)
                                                                                                                          gCCGATTTTAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTtata                      <  1:3360402/150‑1 (MQ=255)
                                                                                                                               ttttAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTc                 <  2:3156669/150‑1 (MQ=255)
                                                                                                                               ttttAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTc                 <  1:3151573/150‑1 (MQ=255)
                                                                                                                                 ttAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTg               <  2:3599668/150‑1 (MQ=255)
                                                                                                                                  tAATGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTgg              >  1:1837624/1‑150 (MQ=255)
                                                                                                                                   aaTGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGt             <  2:1650919/150‑1 (MQ=255)
                                                                                                                                     tGGCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTaa           <  1:532562/150‑1 (MQ=255)
                                                                                                                                      ggCCATTTGGATAGGGACTGTTTTCATTATATCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGTTTCTGATGGCAATAGATGGTATTTTTTCAATTGGTATTTTTAATGTTAATTTATATAGTCTTGTAAt          >  2:962848/1‑150 (MQ=255)
                                                                                                                                       gCCATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATg         >  1:779708/1‑150 (MQ=255)
                                                                                                                                         cATTGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAg       >  1:2100687/1‑150 (MQ=255)
                                                                                                                                            tGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGttt    >  2:353924/1‑150 (MQ=255)
                                                                                                                                            tGGGATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGttt    >  1:786440/1‑150 (MQ=255)
                                                                                                                                             gggATAGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGtttt   <  1:2948005/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTACCTGCCAATAGTTCAAGTAGTGTTATTATATCCATGGTTCTGATGGCAATAGTTGGTATTGTTTCCATTGGTATTTTTAATGGTACTTTATATAGTCTGGTAATGAGTTTTC  >  CP009273/4469871‑4470162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑