Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,482,573 A→T 10.2% L199Q (CTG→CAG)  idnO ← 5‑keto‑D‑gluconate‑5‑reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,482,5730AT10.2% 958.2 / 80.7 304L199Q (CTG→CAG) idnO5‑keto‑D‑gluconate‑5‑reductase
Reads supporting (aligned to +/- strand):  ref base A (130/143);  new base T (9/22);  total (139/165)
Fisher's exact test for biased strand distribution p-value = 5.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 305 total aligned reads displayed.

CCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTCCCAGT  >  CP009273/4482426‑4482722
                                                                                                                                                   |                                                                                                                                                     
 cATCAACAAACAACAGGTGGCCGTTTACGAAATCAGATGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTg                                                                                                                                                    <  1:992340/150‑1 (MQ=255)
    caacaaACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCtt                                                                                                                                                 <  1:1708389/150‑1 (MQ=255)
    caacaaACAACAGGTGGCCGTTTACGAAATCAGAGGCTTGTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCtt                                                                                                                                                 <  2:3654539/150‑1 (MQ=255)
        aaacaaCATGTGGCCGTTTACGAAATCTGAGGCTTTTGAAGAAAGGAACACAGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGt                                                                                                                                             <  1:2399970/150‑1 (MQ=255)
        aaacaaCAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAATGAACTCCGCAGCTCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGTCGGGGGTCAGTTTGCACATCCATGCGGTGAAGGCTTCGTCCTCAAACTGTGCTTTTGt                                                                                                                                             <  2:2632363/150‑1 (MQ=255)
            aacaGGTGGCCGTTTACGAAATCAGAGTCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGTCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCAtt                                                                                                                                         <  1:483769/150‑1 (MQ=255)
            aacaGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCAtt                                                                                                                                         <  1:2994995/150‑1 (MQ=255)
            aacaGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCAtt                                                                                                                                         <  2:1455145/150‑1 (MQ=255)
              caGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTc                                                                                                                                       >  2:2846000/1‑150 (MQ=255)
                ggTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTg                                                                                                                                     <  2:1361978/150‑1 (MQ=255)
                ggTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTg                                                                                                                                     >  2:1535585/1‑150 (MQ=255)
                  tGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGtt                                                                                                                                   >  2:3635735/1‑150 (MQ=255)
                   ggCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGttt                                                                                                                                  <  1:1573172/150‑1 (MQ=255)
                    gCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGtttt                                                                                                                                 <  2:3223038/150‑1 (MQ=255)
                     ccGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTg                                                                                                                                >  1:3700310/1‑150 (MQ=255)
                     ccGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTg                                                                                                                                <  1:220030/150‑1 (MQ=255)
                     ccGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTg                                                                                                                                >  2:2562665/1‑150 (MQ=255)
                        tttACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGaaa                                                                                                                             >  1:2782309/1‑150 (MQ=255)
                        tttACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGaaa                                                                                                                             <  1:2256074/150‑1 (MQ=255)
                          tACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATa                                                                                                                           <  1:446604/150‑1 (MQ=255)
                          tACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATa                                                                                                                           <  1:550406/150‑1 (MQ=255)
                               aaTCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCg                                                                                                                      <  1:2946957/150‑1 (MQ=255)
                                aTCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCgg                                                                                                                     >  2:569175/1‑150 (MQ=255)
                                  cAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGTGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGgcg                                                                                                                   <  2:3188102/150‑1 (MQ=255)
                                   agagGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGgcgc                                                                                                                  >  1:190616/1‑150 (MQ=255)
                                    gagGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCa                                                                                                                 <  2:4293239/150‑1 (MQ=255)
                                      ggCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAAt                                                                                                               <  1:2510647/150‑1 (MQ=255)
                                       gCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATa                                                                                                              <  1:3420867/150‑1 (MQ=255)
                                         ttttGAAGAAATGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATAcc                                                                                                            <  1:3080571/150‑1 (MQ=255)
                                          tttGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCg                                                                                                           <  2:4497428/150‑1 (MQ=255)
                                          tttGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCg                                                                                                           <  2:3237546/150‑1 (MQ=255)
                                              aagaaAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGa                                                                                                       >  2:3887965/1‑150 (MQ=255)
                                                gaaAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGAcc                                                                                                     >  1:3221084/1‑150 (MQ=255)
                                                 aaaGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCt                                                                                                    <  2:1189082/150‑1 (MQ=255)
                                                  aaGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTg                                                                                                   <  1:4052571/150‑1 (MQ=255)
                                                  aaGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTg                                                                                                   <  2:4289655/150‑1 (MQ=255)
                                                   aGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGa                                                                                                  >  2:657890/1‑150 (MQ=255)
                                                    ggAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCACTACCGTTGACCTGaa                                                                                                 >  2:3563629/1‑150 (MQ=255)
                                                    ggAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGaa                                                                                                 >  1:1172830/1‑150 (MQ=255)
                                                      aaCACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAata                                                                                               >  1:2605474/1‑150 (MQ=255)
                                                       acacCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAatat                                                                                              >  1:4118056/1‑150 (MQ=255)
                                                       acacCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAatat                                                                                              >  1:357623/1‑150 (MQ=255)
                                                        cacCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATAtt                                                                                             <  2:2778856/150‑1 (MQ=255)
                                                         acCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATtg                                                                                            >  1:4412004/1‑150 (MQ=255)
                                                          ccGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATtgt                                                                                           >  2:2953595/1‑150 (MQ=255)
                                                            gcagcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTgg                                                                                         <  1:1598770/150‑1 (MQ=255)
                                                            gcagcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTgg                                                                                         >  1:1996187/1‑150 (MQ=255)
                                                            gcagcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTgg                                                                                         >  1:810727/1‑150 (MQ=255)
                                                            gcagcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTgg                                                                                         >  2:2914297/1‑150 (MQ=255)
                                                             cagcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGgc                                                                                        <  1:568263/150‑1 (MQ=255)
                                                              agcaCCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGGTGACCTGAATATTGTGgcg                                                                                       >  1:144197/1‑150 (MQ=255)
                                                                     aTCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGc                                                                                >  1:3286300/1‑150 (MQ=255)
                                                                       cAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTc                                                                              >  1:650194/1‑150 (MQ=255)
                                                                            ccTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGc                                                                         >  1:3601304/1‑150 (MQ=255)
                                                                            ccTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGc                                                                         >  2:900332/1‑150 (MQ=255)
                                                                                 ggATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATg                                                                    >  1:3634337/1‑150 (MQ=255)
                                                                                 ggATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATg                                                                    >  1:1524054/1‑150 (MQ=255)
                                                                                    tctcCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCcg                                                                 <  2:239270/150‑1 (MQ=255)
                                                                                     ctcCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCcgc                                                                >  1:436136/1‑150 (MQ=255)
                                                                                     ctcCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCcgc                                                                >  1:4071299/1‑150 (MQ=255)
                                                                                     ctcCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCcgc                                                                >  1:1741451/1‑150 (MQ=255)
                                                                                       ccccAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCgg                                                              <  2:2614971/150‑1 (MQ=255)
                                                                                        cccAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCggg                                                             <  2:3424712/150‑1 (MQ=255)
                                                                                        cccAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCggg                                                             <  2:3425025/150‑1 (MQ=255)
                                                                                           aGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGa                                                          >  2:930452/1‑150 (MQ=255)
                                                                                           aGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGa                                                          >  1:2352829/1‑150 (MQ=255)
                                                                                                 cggcggGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCAttt                                                    >  2:39077/1‑150 (MQ=255)
                                                                                                  ggcggGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCAtttt                                                   >  2:3326958/1‑150 (MQ=255)
                                                                                                  ggcggGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCAtttt                                                   <  2:4049193/150‑1 (MQ=255)
                                                                                                   gcggGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCAttttt                                                  <  2:4176559/150‑1 (MQ=255)
                                                                                                     gggggTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTAc                                                >  1:4164077/1‑150 (MQ=255)
                                                                                                        ggTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAAGATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGc                                             >  1:238398/1‑150 (MQ=255)
                                                                                                        ggTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGc                                             >  1:3970338/1‑150 (MQ=255)
                                                                                                        ggTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGc                                             >  2:2197723/1‑150 (MQ=255)
                                                                                                        ggTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGc                                             >  2:3278563/1‑150 (MQ=255)
                                                                                                            cGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCt                                         <  2:3591292/150‑1 (MQ=255)
                                                                                                             gTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAATCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCtt                                        >  1:4380001/1‑150 (MQ=255)
                                                                                                              tttGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCttt                                       <  2:3760260/150‑1 (MQ=255)
                                                                                                                tGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCg                                     <  1:478926/150‑1 (MQ=255)
                                                                                                                    caACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGc                                 <  1:1313981/150‑1 (MQ=255)
                                                                                                                     aaCCAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCg                                <  2:238601/150‑1 (MQ=255)
                                                                                                                       ccAGGCGGTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGc                              <  1:2612212/150‑1 (MQ=255)
                                                                                                                       ccAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGc                              <  1:2750297/150‑1 (MQ=255)
                                                                                                                         aGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCAt                            >  2:23483/1‑150 (MQ=255)
                                                                                                                         aGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCAt                            <  2:177266/150‑1 (MQ=255)
                                                                                                                             ggTGAAGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAgg                        <  2:2782309/150‑1 (MQ=255)
                                                                                                                              gTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAggg                       <  1:3427125/150‑1 (MQ=255)
                                                                                                                               tGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAgggg                      >  2:1428106/1‑150 (MQ=255)
                                                                                                                                 aaGGCTTCGTCCTCAACCTGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTg                    >  2:1119482/1‑150 (MQ=255)
                                                                                                                                  aGGCTTCGTCCTCCACAAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGAGCAATACCGTTGAGCTGAATTTTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCTCCCCTTTCGATGCGGCATAAGGGGTGa                   <  2:808622/150‑1 (MQ=255)
                                                                                                                                    gCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATg                 <  2:463450/150‑1 (MQ=255)
                                                                                                                                     cTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATgg                <  2:2027327/150‑1 (MQ=255)
                                                                                                                                      ttCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGgt               >  2:2122198/1‑150 (MQ=255)
                                                                                                                                      ttCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGgt               <  1:1199702/150‑1 (MQ=255)
                                                                                                                                       tCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGgtg              <  1:3750321/150‑1 (MQ=255)
                                                                                                                                       tCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGgtg              <  1:2130524/150‑1 (MQ=255)
                                                                                                                                            cTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACg         >  2:2244704/1‑150 (MQ=255)
                                                                                                                                               aaCCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTcc      >  1:1789697/1‑150 (MQ=255)
                                                                                                                                                aCCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTccc     <  1:4222143/150‑1 (MQ=255)
                                                                                                                                                aCCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTccc     >  1:514043/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATATTGTGGCGCGCCAGCTCGACGCACATGCCGCGGGTGAGCATTTTTACCGCCCCTTTCGATGCGGCATAAGGGGTGATGGTGTCACGTCCCAGT  >  CP009273/4482426‑4482722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑