Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,494,721 G→A 91.0% A283T (GCC→ACC)  yjhB → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,494,7210GA91.0% 825.9 / 56.8 267A283T (GCC→ACC) yjhBputative transporter
Reads supporting (aligned to +/- strand):  ref base G (9/15);  new base A (110/133);  total (119/148)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

GATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATA  >  CP009273/4494576‑4494867
                                                                                                                                                 |                                                                                                                                                  
 aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCttt                                                                                                                                               <  2:618327/150‑1 (MQ=255)
 aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCttt                                                                                                                                               <  1:466773/150‑1 (MQ=255)
 aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCttt                                                                                                                                               >  1:2849644/1‑150 (MQ=255)
 aTAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCttt                                                                                                                                               <  2:4583864/150‑1 (MQ=255)
  tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTg                                                                                                                                              <  1:3695405/150‑1 (MQ=255)
   aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGt                                                                                                                                             >  2:3039951/1‑150 (MQ=255)
   aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGt                                                                                                                                             <  2:3404654/150‑1 (MQ=255)
    aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTa                                                                                                                                            >  2:772826/1‑150 (MQ=255)
    aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTa                                                                                                                                            <  2:1131607/150‑1 (MQ=255)
     aCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAg                                                                                                                                           <  1:1344865/150‑1 (MQ=255)
     aCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAg                                                                                                                                           >  1:2250517/1‑150 (MQ=255)
      cGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGt                                                                                                                                          <  2:1335274/150‑1 (MQ=255)
       ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTc                                                                                                                                         <  1:3146366/150‑1 (MQ=255)
         actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCgg                                                                                                                                       <  2:4031743/150‑1 (MQ=255)
         actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCgg                                                                                                                                       <  1:4205091/150‑1 (MQ=255)
         actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCgg                                                                                                                                       >  1:1914186/1‑150 (MQ=255)
         actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCgg                                                                                                                                       >  1:3775784/1‑150 (MQ=255)
         actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCgg                                                                                                                                       >  1:2808720/1‑150 (MQ=255)
            actTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCt                                                                                                                                    >  1:2600442/1‑150 (MQ=255)
                ccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCtaata                                                                                                                                >  1:1740899/1‑150 (MQ=255)
                ccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCtaata                                                                                                                                <  2:1868587/150‑1 (MQ=255)
                 cttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCtaataa                                                                                                                               <  1:637833/150‑1 (MQ=255)
                  ttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATCAc                                                                                                                              >  2:4499735/1‑150 (MQ=255)
                   tcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACt                                                                                                                             >  2:3764772/1‑150 (MQ=255)
                   tccgACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACt                                                                                                                             <  1:1605179/150‑1 (MQ=255)
                         cTGGCAGATAATGGATTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattt                                                                                                                       <  2:2519486/150‑1 (MQ=255)
                          tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattta                                                                                                                      >  1:289280/1‑150 (MQ=255)
                              aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTAtttt                                                                                                                  <  1:4467567/150‑1 (MQ=255)
                                 tAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCt                                                                                                               >  2:3275087/1‑150 (MQ=255)
                                        gTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                                                                                        >  2:3530834/1‑150 (MQ=255)
                                          tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctct                                                                                                      >  2:2725744/1‑150 (MQ=255)
                                           aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                     >  1:1365442/1‑150 (MQ=255)
                                            aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                                                                    <  2:1597746/150‑1 (MQ=255)
                                            aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                                                                    >  2:2389906/1‑150 (MQ=255)
                                             tACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttt                                                                                                   <  1:4487046/150‑1 (MQ=255)
                                                 gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttttttt                                                                                               <  1:764099/150‑1 (MQ=255)
                                                 gTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttttttta                                                                                               <  1:1311791/150‑2 (MQ=255)
                                                   ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAt                                                                                             <  2:3177441/150‑1 (MQ=255)
                                                    gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAtt                                                                                            <  2:2946545/150‑1 (MQ=255)
                                                     tCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAttt                                                                                           <  2:1832714/150‑1 (MQ=255)
                                                      cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTc                                                                                          <  1:4561658/150‑1 (MQ=255)
                                                       aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                         >  2:1731232/1‑150 (MQ=255)
                                                       aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                         <  1:4116126/150‑1 (MQ=255)
                                                            aaCTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaa                                                                                    <  1:516772/150‑1 (MQ=255)
                                                               tctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaaaaa                                                                                 >  2:737529/1‑150 (MQ=255)
                                                                ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAc                                                                                <  1:1067519/150‑1 (MQ=255)
                                                                ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAc                                                                                <  1:432340/150‑1 (MQ=255)
                                                                     gaCAATAGCAGGTTTAGGAACACTGACAGGGACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttc                                                                           >  1:2380336/1‑150 (MQ=255)
                                                                      aCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttct                                                                          <  1:94635/150‑1 (MQ=255)
                                                                      aCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttct                                                                          <  1:3256895/150‑1 (MQ=255)
                                                                         aTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctctt                                                                       <  2:2994336/150‑1 (MQ=255)
                                                                         aTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctctt                                                                       <  2:966221/150‑1 (MQ=255)
                                                                              aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                  >  2:1316485/1‑150 (MQ=255)
                                                                               ggTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGa                                                                 >  2:259395/1‑150 (MQ=255)
                                                                                 tttAGGAACACTGACAGGTACAATATTTTTTGTTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAtt                                                               <  2:2849644/150‑1 (MQ=255)
                                                                                     ggAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGt                                                           >  1:2625040/1‑150 (MQ=255)
                                                                                     ggAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGt                                                           >  2:2798602/1‑150 (MQ=255)
                                                                                     ggAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTATGTGAAAAACTATTATCTTATAGGATTATGt                                                           >  1:2072440/1‑150 (MQ=255)
                                                                                      gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                          >  2:1786033/1‑150 (MQ=255)
                                                                                      gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                          >  2:3377819/1‑150 (MQ=255)
                                                                                        acacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtctc                                                        >  1:1984113/1‑150 (MQ=255)
                                                                                        acacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtctc                                                        <  1:4179936/150‑1 (MQ=255)
                                                                                        acacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtctc                                                        >  1:298583/1‑150 (MQ=255)
                                                                                          acTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCtt                                                      >  2:765003/1‑150 (MQ=255)
                                                                                           cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                     <  1:740652/150‑1 (MQ=255)
                                                                                            tGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                    >  1:3555044/1‑150 (MQ=255)
                                                                                            tGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                    <  2:2352567/150‑1 (MQ=255)
                                                                                            tGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                    <  1:2195732/150‑1 (MQ=255)
                                                                                             gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTGTTTCTGTGAAAAACTCTTCTCTTATAGGAGTATGTCTCTTTgg                                                   >  2:1386695/1‑150 (MQ=255)
                                                                                             gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                   <  1:4451786/150‑1 (MQ=255)
                                                                                               cAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAt                                                 <  2:4478892/150‑1 (MQ=255)
                                                                                                aGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAtt                                                >  1:2641038/1‑150 (MQ=255)
                                                                                                  gTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTATGTGAAAAACTCTTCTATTATAGGATTATGTCTCTTTGGATTaa                                              >  2:3127338/1‑150 (MQ=255)
                                                                                                  gTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTaa                                              >  2:861166/1‑150 (MQ=255)
                                                                                                  gTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTaa                                              >  2:1274163/1‑150 (MQ=255)
                                                                                                    aCAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATg                                            <  2:3557002/150‑1 (MQ=255)
                                                                                                        tatTTTTTGGTTTTGTTGTTGATAAGATTTGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTa                                        <  1:2392292/150‑1 (MQ=255)
                                                                                                        tatTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTa                                        <  1:804925/150‑1 (MQ=255)
                                                                                                        tatTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTa                                        >  2:2711185/1‑150 (MQ=255)
                                                                                                          ttttttGGTTTTGTTGGTGATAAGATTTGTGTAAAAAAAACCTTTTTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCGTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACa                                      <  1:1036417/150‑1 (MQ=255)
                                                                                                             tttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAt                                   >  1:1152017/1‑150 (MQ=255)
                                                                                                               tGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAttt                                 >  1:2392112/1‑150 (MQ=255)
                                                                                                                 gTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAg                               <  2:1914186/150‑1 (MQ=255)
                                                                                                                  ttttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAgg                              <  1:2552535/150‑1 (MQ=255)
                                                                                                                  ttttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAgg                              <  2:4269264/150‑1 (MQ=255)
                                                                                                                  ttttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAgg                              >  2:4411080/1‑150 (MQ=255)
                                                                                                                   tttGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGt                             >  2:2692910/1‑150 (MQ=255)
                                                                                                                       ttgGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTg                         >  1:2716310/1‑150 (MQ=255)
                                                                                                                        tgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGc                        >  1:1847428/1‑150 (MQ=255)
                                                                                                                          gTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAg                      >  2:927893/1‑150 (MQ=255)
                                                                                                                           tGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAgg                     >  2:428932/1‑150 (MQ=255)
                                                                                                                              tAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggtt                  <  2:742868/150‑1 (MQ=255)
                                                                                                                               aaGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                 <  2:3597302/150‑1 (MQ=255)
                                                                                                                               aaGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCAGTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                 >  2:1982715/1‑150 (MQ=255)
                                                                                                                                  aTTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt              >  1:553020/1‑150 (MQ=255)
                                                                                                                                  aTTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt              >  2:382001/1‑150 (MQ=255)
                                                                                                                                   ttGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTc             >  1:4150610/1‑150 (MQ=255)
                                                                                                                                        gtAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCaaaa        >  2:2824440/1‑150 (MQ=255)
                                                                                                                                         tAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAt       <  1:3519360/150‑1 (MQ=255)
                                                                                                                                             aaaaACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtat   >  2:2326481/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATA  >  CP009273/4494576‑4494867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑