Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,514,180 A→T 10.1% A216A (GCT→GCA)  yjhH ← putative lyase/synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,514,1800AT10.1% 905.5 / 76.4 293A216A (GCT→GCA) yjhHputative lyase/synthase
Reads supporting (aligned to +/- strand):  ref base A (139/123);  new base T (16/14);  total (155/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 302 total aligned reads displayed.

ACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGAT  >  CP009273/4514031‑4514329
                                                                                                                                                     |                                                                                                                                                     
acacacTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGa                                                                                                                                                       >  2:797314/1‑150 (MQ=255)
acacacTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGa                                                                                                                                                       >  1:1286072/1‑150 (MQ=255)
acacacTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGa                                                                                                                                                       >  1:4334700/1‑150 (MQ=255)
   cacTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCa                                                                                                                                                    >  2:1739301/1‑150 (MQ=255)
        cATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGtt                                                                                                                                               >  1:3093231/1‑150 (MQ=255)
          tGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAg                                                                                                                                             >  2:3522175/1‑150 (MQ=255)
          tGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAg                                                                                                                                             <  2:2244438/150‑1 (MQ=255)
           gCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAgc                                                                                                                                            <  2:815549/150‑1 (MQ=255)
             tGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAgcgc                                                                                                                                          <  1:1966134/150‑1 (MQ=255)
                aTTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTgg                                                                                                                                       >  2:1400784/1‑150 (MQ=255)
                 tttGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGc                                                                                                                                      >  1:1694856/1‑150 (MQ=255)
                   tGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCgg                                                                                                                                    <  1:4193912/150‑1 (MQ=255)
                      tCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTa                                                                                                                                 >  2:3428239/1‑150 (MQ=255)
                       cAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTAt                                                                                                                                >  2:1189819/1‑150 (MQ=255)
                           gagaCAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCa                                                                                                                            <  2:2788334/150‑1 (MQ=255)
                             gaCAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTCTTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCAcc                                                                                                                          <  1:2090273/150‑1 (MQ=255)
                             gaCAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCAcc                                                                                                                          <  1:3287927/150‑1 (MQ=255)
                                aaaCGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTc                                                                                                                       >  1:2646935/1‑150 (MQ=255)
                                aaaCGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTc                                                                                                                       >  1:296961/1‑150 (MQ=255)
                                  aCGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTcgc                                                                                                                     >  1:3426117/1‑150 (MQ=255)
                                    ggTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTcgccg                                                                                                                   >  1:2540285/1‑150 (MQ=255)
                                    ggTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTcgccg                                                                                                                   >  2:3991525/1‑150 (MQ=255)
                                    ggTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTcgccg                                                                                                                   >  2:3362950/1‑150 (MQ=255)
                                       gtTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGccc                                                                                                                >  1:864582/1‑150 (MQ=255)
                                        tttCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCca                                                                                                               <  2:1242710/150‑1 (MQ=255)
                                           cGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCcagca                                                                                                            >  1:3680196/1‑150 (MQ=255)
                                            gAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCcagcag                                                                                                           >  2:1591305/1‑150 (MQ=255)
                                            gAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCcagcag                                                                                                           <  1:1483431/150‑1 (MQ=255)
                                              gggCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAgcagca                                                                                                         >  2:2045261/1‑150 (MQ=255)
                                               ggCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCAt                                                                                                        >  1:2189818/1‑150 (MQ=255)
                                                gCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATc                                                                                                       <  2:3782862/150‑1 (MQ=255)
                                                  gTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGt                                                                                                     >  1:2489354/1‑150 (MQ=255)
                                                   tAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTa                                                                                                    >  2:447845/1‑150 (MQ=255)
                                                   tAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTa                                                                                                    >  1:3723602/1‑150 (MQ=255)
                                                   tAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTa                                                                                                    >  2:1223686/1‑150 (MQ=255)
                                                   tAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTa                                                                                                    >  2:2364718/1‑150 (MQ=255)
                                                     aaTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACTCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTAtt                                                                                                  <  1:3028968/150‑1 (MQ=255)
                                                      aTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTc                                                                                                 <  2:4406863/150‑1 (MQ=255)
                                                      aTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTCTTc                                                                                                 >  1:2879945/1‑150 (MQ=255)
                                                      aTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTc                                                                                                 >  1:672558/1‑150 (MQ=255)
                                                      aTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTc                                                                                                 >  1:563309/1‑150 (MQ=255)
                                                       tAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCa                                                                                                <  2:2215546/150‑1 (MQ=255)
                                                       tAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCa                                                                                                <  2:2867414/150‑1 (MQ=255)
                                                       tAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCa                                                                                                <  2:3063602/150‑1 (MQ=255)
                                                         gCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGc                                                                                              >  2:3062412/1‑150 (MQ=255)
                                                         gCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGc                                                                                              >  2:4131248/1‑150 (MQ=255)
                                                                   gtagtaGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGatcat                                                                                    <  1:4445894/150‑1 (MQ=255)
                                                                   gtagtaGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGatcat                                                                                    <  1:3877651/150‑1 (MQ=255)
                                                                   gtagtaGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGatcat                                                                                    >  1:3622674/1‑150 (MQ=255)
                                                                     agtagtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCg                                                                                  <  2:1469458/150‑1 (MQ=255)
                                                                      gtagtTTTTTATTCAGCATCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGt                                                                                 <  2:4450102/150‑1 (MQ=255)
                                                                        agtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTaa                                                                               >  2:3615096/1‑150 (MQ=255)
                                                                        agtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTaa                                                                               >  1:1647236/1‑150 (MQ=255)
                                                                        agtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTaa                                                                               >  2:470128/1‑150 (MQ=255)
                                                                           tttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCg                                                                            >  2:1925416/1‑150 (MQ=255)
                                                                            ttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGc                                                                           >  1:1108915/1‑150 (MQ=255)
                                                                              ttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAg                                                                         <  1:1050004/150‑1 (MQ=255)
                                                                              ttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAg                                                                         >  1:2571336/1‑150 (MQ=255)
                                                                               tATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGa                                                                        >  1:2795338/1‑150 (MQ=255)
                                                                                aTTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGaa                                                                       <  2:2715213/150‑1 (MQ=255)
                                                                                 ttCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAAt                                                                      >  2:3450689/1‑150 (MQ=255)
                                                                                    aGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATAcc                                                                   >  1:3367118/1‑150 (MQ=255)
                                                                                      cGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGa                                                                 <  1:3525673/150‑1 (MQ=255)
                                                                                          gCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCTGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAac                                                             >  1:2277900/1‑150 (MQ=255)
                                                                                          gCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAac                                                             <  2:1787517/150‑1 (MQ=255)
                                                                                          gCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAac                                                             <  2:2755520/150‑1 (MQ=255)
                                                                                          gCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAac                                                             <  2:3743711/150‑1 (MQ=255)
                                                                                           cAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                            <  2:2388728/150‑1 (MQ=255)
                                                                                            aGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacga                                                           >  1:3894709/1‑150 (MQ=255)
                                                                                            aGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacga                                                           >  1:270007/1‑150 (MQ=255)
                                                                                               ggTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACgg                                                        <  2:628107/150‑1 (MQ=255)
                                                                                               ggTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACgg                                                        <  2:2991007/150‑1 (MQ=255)
                                                                                                gTCGCCAGATCGCCTTCACGCCTGGCGCGGTTGATGCCGAGGGAGAGTTCCGGAGCAATGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCTTCGTATTCAGCAATTGATCATCGTAACCGCAGAATACCGAAAACGACggg                                                       <  2:1558683/150‑1 (MQ=255)
                                                                                                gTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACggg                                                       >  1:2307913/1‑150 (MQ=255)
                                                                                                 tCGCCAGATCGCCTTCACGCCAGGCGCGGTTGATGCCGACGGTGAGTTCCGGAGCAAAGTTAGCGCTGGGGTTTATCGCACCGTCGCCGCCCAGCAGAATCTTATTCAGAAAATGATCATCTTAACCGCAGAATACCGAAAACGACGGGc                                                      <  1:490600/150‑1 (MQ=255)
                                                                                                   gCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGt                                                    <  2:678412/150‑1 (MQ=255)
                                                                                                     cAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACTACGGGCGAAc                                                  >  2:675318/1‑150 (MQ=255)
                                                                                                       gATCGCCATCACGCCAGGCGCGGAAGAAGCCCACGGAGACATCCGGAGCAAAGTAAGCGCAGACGGTTATCGCACCGTCGCCGCCCATCAGCAACGTATTCAGCAAATGATCAACGAAACCGCAGAATACCGAAAACGACACGCGAACCg                                                >  2:3839823/1‑150 (MQ=255)
                                                                                                            ccTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTa                                           >  1:1806067/1‑150 (MQ=255)
                                                                                                             cTTCACGCCAGGCGCGGTAGATGCCGACGGCGAGTTCCGGCGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCTTATCCAGAAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTaa                                          <  1:463571/150‑1 (MQ=255)
                                                                                                                cACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAATGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCCGCATCGTATTCTGCAAATGATAATCGTAACCGCAGATTACCGAAAACGACGGGCGTACCGACTTAACtg                                       <  2:2538428/150‑1 (MQ=255)
                                                                                                                cACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTCGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACtg                                       <  2:4053129/150‑1 (MQ=255)
                                                                                                                 aCGCCAGGCGCGGTAGATGCCGACGTAGAGTCCCGGAGCAAATTTAGCGCTGGCGTTTATCGCACCGTCGCCGCCCAGCAGCAACGTATTAAGCAAATGATCATCTTAACCGCATAATACCGAAAACGACGGGCGTACCGACTTAACtgt                                      <  1:1174697/150‑1 (MQ=255)
                                                                                                                 aCGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACtgt                                      >  2:1998418/1‑150 (MQ=255)
                                                                                                                     cAGGCGCGGTTGATGCCGACGGAGAGTTCCGGTGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTg                                  <  1:3864559/150‑1 (MQ=255)
                                                                                                                         cgcgGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGatca                              >  2:2947657/1‑150 (MQ=255)
                                                                                                                              tAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTa                         >  1:1354764/1‑150 (MQ=255)
                                                                                                                              tAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTa                         <  1:4443636/150‑1 (MQ=255)
                                                                                                                               aGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTAc                        <  1:325167/150‑1 (MQ=255)
                                                                                                                                gATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACg                       <  2:2679208/150‑1 (MQ=255)
                                                                                                                                gATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACg                       <  1:1834378/150‑1 (MQ=255)
                                                                                                                                 aTGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGc                      >  1:2408889/1‑150 (MQ=255)
                                                                                                                                         ggAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGAcc              >  1:4238195/1‑150 (MQ=255)
                                                                                                                                         ggAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGAcc              >  2:4586615/1‑150 (MQ=255)
                                                                                                                                           agagTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCaa            >  2:1002401/1‑150 (MQ=255)
                                                                                                                                             agTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACg          <  2:3254231/150‑1 (MQ=255)
                                                                                                                                               ttCCGGAGCAATGTTAGCGCGGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTACCCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCt        <  1:3244079/150‑1 (MQ=255)
                                                                                                                                                  cGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTc     <  1:3854076/150‑1 (MQ=255)
                                                                                                                                                   ggAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCg    >  2:14074/1‑150 (MQ=255)
                                                                                                                                                    gAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCCGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGa   <  2:3874614/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGAT  >  CP009273/4514031‑4514329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑