BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,572,227	G→T	55.9%	I213I (ATC→ATA)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,572,227	0	G	T	55.9%	8.2 / inf	241	I213I (ATC→ATA)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base G (40/66); new base T (49/86); total (89/152)
Fisher's exact test for biased strand distribution p-value = 8.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01

Only 100 of 241 total aligned reads displayed.

ATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTT  >  CP009273/4572083‑4572375

atGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATaaa                                                                                                                                                 >  2:1461996/1‑150 (MQ=255)

  gcgcCTTCGGCAGGTTTTCACCGTCGCTACNCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcg                                                                                                                                               >  1:4285491/1‑150 (MQ=255)

    gcCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcgcg                                                                                                                                             >  1:4105590/1‑150 (MQ=255)

      cTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAAcgcgcg                                                                                                                                           <  2:2779464/150‑1 (MQ=255)

         cGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTg                                                                                                                                        <  1:1022076/150‑1 (MQ=255)

          ggCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTgg                                                                                                                                       <  1:3142537/150‑1 (MQ=255)

          ggCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTgg                                                                                                                                       <  2:3710172/150‑1 (MQ=255)

           gCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGa                                                                                                                                      <  1:638412/150‑1 (MQ=255)

            cAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGAt                                                                                                                                     <  1:4139812/150‑1 (MQ=255)

            cAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGAt                                                                                                                                     <  1:4139766/150‑1 (MQ=255)

               gTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTg                                                                                                                                  <  1:826838/150‑1 (MQ=255)

               gTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTg                                                                                                                                  <  2:4097445/150‑1 (MQ=255)

                          cGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCg                                                                                                                       <  2:2177299/150‑1 (MQ=255)

                          cGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCg                                                                                                                       >  1:1018549/1‑150 (MQ=255)

                          cGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCg                                                                                                                       <  1:2605367/150‑1 (MQ=255)

                              aCCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTc                                                                                                                   >  1:2697740/1‑150 (MQ=255)

                                ccAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGAGTGTCGc                                                                                                                 >  2:4401302/1‑150 (MQ=255)

                                 cAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGcc                                                                                                                >  1:2683889/1‑150 (MQ=255)

                                  agagTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCa                                                                                                               >  2:3587488/1‑150 (MQ=255)

                                      tgtTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCaa                                                                                                           >  1:359019/1‑150 (MQ=255)

                                       gtTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAg                                                                                                          <  2:3886333/150‑1 (MQ=255)

                                       gtTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAg                                                                                                          <  2:3094367/150‑1 (MQ=255)

                                       gtTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAg                                                                                                          <  2:2292865/150‑1 (MQ=255)

                                         tGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGgt                                                                                                        <  2:3741470/150‑1 (MQ=255)

                                         tGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGgt                                                                                                        <  1:3558127/150‑1 (MQ=255)

                                          gCCTAGAAGGATTGAGCCGCCGTGGTAGTGGTTGCCTTGAATATCGTGCTGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCTCAAGTTCGAGGCCTATAACCGCGCGGTGGATCTGTAAATCCTTCGTGTCGCCACCACGgtc                                                                                                       <  2:114689/150‑1 (MQ=255)

                                          gCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGgtc                                                                                                       >  1:1687400/1‑150 (MQ=255)

                                            ccAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGgtcgt                                                                                                     <  1:4581896/150‑1 (MQ=255)

                                                aaggATTGCGCCGCCGTGGTCGAGTTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAg                                                                                                 >  2:1275459/3‑150 (MQ=255)

                                                aCGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAg                                                                                                 <  2:2391371/150‑1 (MQ=255)

                                                aCGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAg                                                                                                 >  1:1349507/1‑150 (MQ=255)

                                                   gATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATc                                                                                              <  1:2833868/150‑1 (MQ=255)

                                                   gATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATc                                                                                              <  1:247840/150‑1 (MQ=255)

                                                       gcgcCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTg                                                                                          <  1:2924947/150‑1 (MQ=255)

                                                         gccgccGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGt                                                                                        >  2:2588169/1‑150 (MQ=255)

                                                          ccgccgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGtt                                                                                       >  2:4508855/1‑150 (MQ=255)

                                                          ccgccgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGtt                                                                                       <  1:104321/150‑1 (MQ=255)

                                                            gccgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTg                                                                                     >  1:4438769/1‑150 (MQ=255)

                                                             ccgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGc                                                                                    <  1:3561289/150‑1 (MQ=255)

                                                              cgTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCg                                                                                   <  2:683769/150‑1 (MQ=255)

                                                                tGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGAc                                                                                 <  2:4438092/150‑1 (MQ=255)

                                                                 ggTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACt                                                                                <  2:3235762/150‑1 (MQ=255)

                                                                   tCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTa                                                                              <  1:2255175/150‑1 (MQ=255)

                                                                   tCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTa                                                                              <  2:65361/150‑1 (MQ=255)

                                                                    cGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTaa                                                                             <  2:2522489/150‑1 (MQ=255)

                                                                    cGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTaa                                                                             <  2:849150/150‑1 (MQ=255)

                                                                     gAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAAc                                                                            <  2:2127056/150‑1 (MQ=255)

                                                                     gAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAAc                                                                            >  2:2402609/1‑150 (MQ=255)

                                                                        gTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATa                                                                         <  1:1325052/150‑1 (MQ=255)

                                                                             cTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGt                                                                    <  2:3476865/150‑1 (MQ=255)

                                                                              ttCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTc                                                                   <  1:21863/150‑1 (MQ=255)

                                                                              ttCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTc                                                                   <  1:1373319/150‑1 (MQ=255)

                                                                               tCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCg                                                                  <  1:1784193/150‑1 (MQ=255)

                                                                               tCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCg                                                                  <  1:507456/150‑1 (MQ=255)

                                                                               tCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCg                                                                  <  2:176083/150‑1 (MQ=255)

                                                                                cAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCgg                                                                 >  2:359589/1‑150 (MQ=255)

                                                                                   tatCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCtt                                                              <  1:2458444/150‑1 (MQ=255)

                                                                                   tatCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCtt                                                              <  2:1749588/150‑1 (MQ=255)

                                                                                    atCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTtc                                                             >  2:1886432/1‑150 (MQ=255)

                                                                                               ggcagTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGc                                                  <  1:1209862/150‑1 (MQ=255)

                                                                                                gcagTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGcc                                                 <  1:3507076/150‑1 (MQ=255)

                                                                                                 cagTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGccc                                                <  2:3733182/150‑1 (MQ=255)

                                                                                                  agTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccg                                               >  2:2266199/1‑150 (MQ=255)

                                                                                                  agTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccg                                               >  1:4193202/1‑150 (MQ=255)

                                                                                                    ttCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccgcc                                             >  2:1310283/1‑150 (MQ=255)

                                                                                                    ttCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccgcc                                             <  2:1321399/150‑1 (MQ=255)

                                                                                                    ttCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccgcc                                             <  1:3302596/150‑1 (MQ=255)

                                                                                                     tcatcaGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCccgccg                                            <  1:1460112/150‑1 (MQ=255)

                                                                                                       atcaGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTa                                          <  2:1123311/150‑1 (MQ=255)

                                                                                                        tcaGTGCCAGACGACGGGTGCCTGGCACCAGTTCGAGGCCGATAAACTCGCGGTTGTTCTGGAATTCCTGCGTGTCGCCATCAAGGTCGTCCTGATCTTTGGTTTGAGACTTAACATAGCGGTGGGCTTCATTCAAATAGCCCGCCGTAc                                         <  2:4153561/150‑1 (MQ=255)

                                                                                                        tcaGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTAc                                         <  2:1917581/150‑1 (MQ=255)

                                                                                                        tcaGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTAc                                         >  2:2842139/1‑150 (MQ=255)

                                                                                                        tcaGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTAc                                         <  2:743997/150‑1 (MQ=255)

                                                                                                           gTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTg                                      <  2:2321230/150‑1 (MQ=255)

                                                                                                             gCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTgcc                                    <  2:2629339/150‑1 (MQ=255)

                                                                                                                 gacgacGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGccgccg                                >  2:1851590/1‑150 (MQ=255)

                                                                                                                  acgacgGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCgg                               <  2:4023339/150‑1 (MQ=255)

                                                                                                                  acgacgGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCgg                               >  1:426682/1‑150 (MQ=255)

                                                                                                                   cgacgGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCggg                              >  1:1933334/1‑150 (MQ=255)

                                                                                                                    gacgGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGt                             >  1:2881698/1‑150 (MQ=255)

                                                                                                                    gacgGGTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGt                             >  1:3917333/1‑150 (MQ=255)

                                                                                                                    gacgGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGt                             <  1:2179140/150‑1 (MQ=255)

                                                                                                                       gggTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCt                          <  2:1526473/150‑1 (MQ=255)

                                                                                                                       gggTGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCt                          >  2:1572828/1‑150 (MQ=255)

                                                                                                                          tGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGca                       >  2:3392049/1‑150 (MQ=255)

                                                                                                                          tGCCGGGCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGca                       >  2:2451144/1‑150 (MQ=255)

                                                                                                                            ccGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGcacc                     >  1:4055170/1‑150 (MQ=255)

                                                                                                                              gggCACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGcaccac                   >  2:430706/1‑150 (MQ=255)

                                                                                                                              gggCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGcaccac                   >  1:3764926/1‑150 (MQ=255)

                                                                                                                                 cACCAGTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTc                <  2:3918084/150‑1 (MQ=255)

                                                                                                                                  aCCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCa               <  1:766339/150‑1 (MQ=255)

                                                                                                                                  aCCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCa               <  1:3061619/150‑1 (MQ=255)

                                                                                                                                  aCCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCa               >  2:4480422/1‑150 (MQ=255)

                                                                                                                                     aGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCAcgc            >  1:3347059/1‑150 (MQ=255)

                                                                                                                                      gTTCGAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCAcgcg           <  1:2365785/150‑1 (MQ=255)

                                                                                                                                       ttCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCgg          >  2:1007940/1‑150 (MQ=255)

                                                                                                                                         cGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCt        >  2:3049069/1‑150 (MQ=255)

                                                                                                                                          gAGGCCTATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTg       <  1:1559891/150‑1 (MQ=255)

                                                                                                                                           aGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGc      <  1:1216257/150‑1 (MQ=255)

                                                                                                                                               cGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGtt  >  2:2938650/1‑150 (MQ=255)

ATGCGCCTTCGGCAGGTTTTCACCGTCGCTACCCAGAGTGTTGCCCAGACGGATTGCGCCGCCGTGGTCGAGGTTGCCTTCAATATCGTGCAGCAGGCAGTTCATCAGTGCCAGACGACGGGTGCCGGGCACCAGTTCGAGGCCGATAAACGCGCGGTGGATCTGGAAATCCTGCGTGTCGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTT  >  CP009273/4572083‑4572375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`