Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,572,800 A→T 15.0% V22V (GTT→GTA)  hsdM ← DNA methyltransferase M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,572,8000AT15.0% 649.1 / 94.4 226V22V (GTT→GTA) hsdMDNA methyltransferase M
Reads supporting (aligned to +/- strand):  ref base A (102/90);  new base T (16/18);  total (118/108)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 230 total aligned reads displayed.

CGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATC  >  CP009273/4572651‑4572947
                                                                                                                                                     |                                                                                                                                                   
cGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGaa                                                                                                                                                     >  2:104643/1‑150 (MQ=255)
cGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGaa                                                                                                                                                     >  1:3392063/1‑150 (MQ=255)
     gAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAAcgcc                                                                                                                                                >  2:3465744/1‑150 (MQ=255)
         tgcaactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCa                                                                                                                                            >  2:1976038/1‑150 (MQ=255)
          gcaactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCAt                                                                                                                                           >  1:1514748/1‑150 (MQ=255)
          gcaactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCAt                                                                                                                                           <  2:1930706/150‑1 (MQ=255)
          gcaactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCAt                                                                                                                                           >  1:2295514/1‑150 (MQ=255)
          gcaactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCAt                                                                                                                                           <  1:3446541/150‑1 (MQ=255)
           caactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCATGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATc                                                                                                                                          <  2:4121178/150‑1 (MQ=255)
           caactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATc                                                                                                                                          <  2:2602063/150‑1 (MQ=255)
            aactgcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATcg                                                                                                                                         >  2:459937/1‑150 (MQ=255)
              ctgcTCCTGGTCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATcgcg                                                                                                                                       <  2:590085/150‑1 (MQ=255)
                gcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCa                                                                                                                                     >  1:31987/1‑150 (MQ=255)
                gcTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCa                                                                                                                                     >  1:545347/1‑150 (MQ=255)
                    cTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGtt                                                                                                                                 >  2:4077342/1‑150 (MQ=255)
                      ggCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGt                                                                                                                               >  1:3737515/1‑150 (MQ=255)
                           aTGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcac                                                                                                                          <  1:3168558/150‑1 (MQ=255)
                            tGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcaca                                                                                                                         >  2:1540585/1‑150 (MQ=255)
                              cGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcacagc                                                                                                                       >  1:3672349/1‑150 (MQ=255)
                              cGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcacagc                                                                                                                       >  1:2232054/1‑150 (MQ=255)
                               gggATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcacagct                                                                                                                      >  2:1427425/1‑150 (MQ=255)
                                ggATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcacagctt                                                                                                                     <  2:1725783/150‑1 (MQ=255)
                                         gTCATCCCAGCGGTAACCTTCCGGCTGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcacagcttcctctgctt                                                                                                            <  2:445745/150‑1 (MQ=255)
                                         gTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcacagcttccacagctt                                                                                                            >  1:3678594/1‑150 (MQ=255)
                                         gTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTC‑AAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGcacagcttccacagcttc                                                                                                           <  2:4197364/150‑1 (MQ=255)
                                           cATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTcg                                                                                                          >  1:1608642/1‑150 (MQ=255)
                                           cATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTcg                                                                                                          <  1:711466/150‑1 (MQ=255)
                                           cATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTcg                                                                                                          <  1:4416685/150‑1 (MQ=255)
                                                   cGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGa                                                                                                  >  1:3976027/1‑150 (MQ=255)
                                                    ggTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGAt                                                                                                 >  2:156667/1‑150 (MQ=255)
                                                    ggTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGAt                                                                                                 <  2:1822388/150‑1 (MQ=255)
                                                       aaCCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGt                                                                                              >  1:2607052/1‑150 (MQ=255)
                                                          cTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTAt                                                                                           <  2:1484626/150‑1 (MQ=255)
                                                          cTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTAt                                                                                           >  2:864936/1‑150 (MQ=255)
                                                           ttCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTAtt                                                                                          <  1:3475491/150‑1 (MQ=255)
                                                              cGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGtt                                                                                       >  2:4328286/1‑150 (MQ=255)
                                                              cGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTCCCACAGCTTCGCGACCAGATCGTTATTGtt                                                                                       <  1:4278389/150‑1 (MQ=255)
                                                                  aGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCAtt                                                                                   >  1:2338198/1‑150 (MQ=255)
                                                                      aTTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGa                                                                               >  2:3139437/1‑150 (MQ=255)
                                                                         ccGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGtt                                                                            >  2:3728314/1‑150 (MQ=255)
                                                                            cTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCg                                                                         <  2:1241777/150‑1 (MQ=255)
                                                                             ttCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGt                                                                        >  2:3303455/1‑150 (MQ=255)
                                                                              tCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTa                                                                       <  2:2290223/150‑1 (MQ=255)
                                                                               ccTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTaa                                                                      <  1:768401/150‑1 (MQ=255)
                                                                               ccTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTaa                                                                      >  1:2124622/1‑150 (MQ=255)
                                                                                cTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTaaa                                                                     >  2:2395918/1‑150 (MQ=255)
                                                                                 tGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAAt                                                                    <  2:4029104/150‑1 (MQ=255)
                                                                                 tGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAAt                                                                    <  1:3493978/150‑1 (MQ=255)
                                                                                 tGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAAt                                                                    <  1:3035959/150‑1 (MQ=255)
                                                                                  gACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAAtt                                                                   >  1:4554537/1‑150 (MQ=255)
                                                                                  gACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAAtt                                                                   >  2:3698653/1‑150 (MQ=255)
                                                                                  gACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAAtt                                                                   >  2:3698610/1‑150 (MQ=255)
                                                                                   aCCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTa                                                                  >  2:1153640/1‑150 (MQ=255)
                                                                                   aCCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTa                                                                  >  1:290728/1‑150 (MQ=255)
                                                                                     cGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAag                                                                >  1:3617503/1‑150 (MQ=255)
                                                                                     cGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAag                                                                >  1:4488132/1‑150 (MQ=255)
                                                                                     cGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAag                                                                >  1:434594/1‑150 (MQ=255)
                                                                                      ggTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAagc                                                               <  2:1290691/150‑1 (MQ=255)
                                                                                      ggTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAagc                                                               <  2:2935500/150‑1 (MQ=255)
                                                                                      ggTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAagc                                                               <  2:3614876/150‑1 (MQ=255)
                                                                                       gTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAagca                                                              <  1:4096276/150‑1 (MQ=255)
                                                                                          tctTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCg                                                           >  1:3156438/1‑150 (MQ=255)
                                                                                            tttACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGc                                                         >  2:2913384/1‑150 (MQ=255)
                                                                                              tACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGccc                                                       >  1:3898232/1‑150 (MQ=255)
                                                                                               acacATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCa                                                      <  1:2418304/150‑1 (MQ=255)
                                                                                               acacATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCa                                                      <  2:934562/150‑1 (MQ=255)
                                                                                               acacATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCa                                                      >  1:1094640/1‑150 (MQ=255)
                                                                                                  caTTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAAt                                                   <  2:4352420/150‑1 (MQ=255)
                                                                                                    ttttCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTc                                                 <  2:3265409/150‑1 (MQ=255)
                                                                                                      ttCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCAt                                               >  1:979151/1‑150 (MQ=255)
                                                                                                        cAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCg                                             <  2:2308423/150‑1 (MQ=255)
                                                                                                         aaaaaCATCAGCGAGGCGAGTTCATTGACGGAGTTTTGATAGTAAACTCCGCCATCGAGCAGTTTGTCGCACAGCTTCCACAGCTTCGCGACAAGATCGTTATTGTTAATTGTCAGTTCCGTAAATTAATCAGCGGCCCAAATTCATAGa                                            >  1:3894279/1‑150 (MQ=255)
                                                                                                         aaaaaCAGCAGCGAGGCGAGTTCTTTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGa                                            <  1:4229620/150‑1 (MQ=255)
                                                                                                         aaaaaCAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGa                                            >  1:4080336/1‑150 (MQ=255)
                                                                                                          aaaaCAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAg                                           >  1:2077620/1‑150 (MQ=255)
                                                                                                            aaCAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGcc                                         >  1:1259311/1‑150 (MQ=255)
                                                                                                                gcagcGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCag                                     <  2:2265588/150‑1 (MQ=255)
                                                                                                                 cagcGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCaga                                    <  1:459937/150‑1 (MQ=255)
                                                                                                                     gaggcgagTTCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCagaagaa                                <  2:2685308/150‑1 (MQ=255)
                                                                                                                     gaggcgagTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCagaagaa                                >  2:2286711/1‑150 (MQ=255)
                                                                                                                     gaggcgagTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCagaagaa                                >  1:44798/1‑150 (MQ=255)
                                                                                                                         cgagTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGaaa                            <  2:3573751/150‑1 (MQ=255)
                                                                                                                              tCATTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGcc                       <  2:205123/150‑1 (MQ=255)
                                                                                                                              tCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGcc                       <  1:2477746/150‑1 (MQ=255)
                                                                                                                                aTTGACGTAGTTTTGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGa                     >  1:219492/1‑150 (MQ=255)
                                                                                                                                 ttGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAg                    <  2:4363023/150‑1 (MQ=255)
                                                                                                                                     cttAGTTTTGATAGGAAACGCAGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGATTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTa                <  1:1774813/148‑1 (MQ=255)
                                                                                                                                       tAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAat              <  2:635766/150‑1 (MQ=255)
                                                                                                                                       tAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAat              >  2:2507723/1‑150 (MQ=255)
                                                                                                                                        aGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAata             <  2:3885296/150‑1 (MQ=255)
                                                                                                                                          ttttGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAAtata           <  1:873301/150‑1 (MQ=255)
                                                                                                                                          ttttGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAAtata           <  2:2861079/150‑1 (MQ=255)
                                                                                                                                          ttttGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAAtata           <  1:1066552/150‑1 (MQ=255)
                                                                                                                                             tGATAGGATACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATacac        <  2:844082/150‑1 (MQ=255)
                                                                                                                                             tGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATacac        >  1:1070244/1‑150 (MQ=255)
                                                                                                                                              gATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATAcaca       >  1:2641958/1‑150 (MQ=255)
                                                                                                                                               aTAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACaa      >  2:4221953/1‑150 (MQ=255)
                                                                                                                                                tAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACaaa     <  2:3976027/150‑1 (MQ=255)
                                                                                                                                                 aGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACaaaa    <  1:270001/150‑1 (MQ=255)
                                                                                                                                                 aGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGGTATTGTTCATTGTGAGTTCCGGAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTCATATACACaaaa    >  2:1212137/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTTCCTGACCGGTCTCTTTACACATTTTCAAAAACAGCAGCGAGGCGAGTTCATTGACGTAGTTTTGATAGGAAACGCCGCCATCGCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATC  >  CP009273/4572651‑4572947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑