BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,596,008	C→A	22.7%	intergenic (‑26/+9)	leuV ← / ← leuP	tRNA‑Leu/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,596,008	0	C	A	22.7%	409.9 / 84.9	179	intergenic (‑26/+9)	leuV/leuP	tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base C (61/75); new base A (17/23); total (81/98)
Fisher's exact test for biased strand distribution p-value = 8.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 182 total aligned reads displayed.

AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCC  >  CP009273/4595859‑4596157

aaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATc                                                                                                                                                       <  1:2802181/150‑1 (MQ=255)

     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTc                                                                                                                                                  <  2:2991056/150‑1 (MQ=255)

     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTc                                                                                                                                                  <  2:671219/150‑1 (MQ=255)

        gaataaACAAGAAAGGAAGTAAATATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACTCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGtt                                                                                                                                               <  1:3259828/150‑1 (MQ=255)

        gaagaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCATCGtt                                                                                                                                               >  2:3316283/1‑150 (MQ=255)

           gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTgg                                                                                                                                            <  1:4397227/150‑1 (MQ=255)

           gaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTgg                                                                                                                                            <  2:905092/150‑1 (MQ=255)

            aaaCAAGAAAGGAAGTAAATTTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACTCCTGAAGCTAGCGAGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGt                                                                                                                                           <  1:1030778/150‑1 (MQ=255)

             aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTg                                                                                                                                          <  2:1695826/150‑1 (MQ=255)

             aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTg                                                                                                                                          <  1:4409235/150‑1 (MQ=255)

             aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTg                                                                                                                                          <  2:3299417/150‑1 (MQ=255)

              aCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTAC‑TTTTTTTGA‑TATAGCGTCGTTTGGTGc                                                                                                                                         >  1:3129559/1‑150 (MQ=255)

                 aGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAg                                                                                                                                      <  1:3828700/150‑1 (MQ=255)

                  gAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTAC‑TTTGTTAGA‑TATAGCCTCGTGTGGTGCGAgg                                                                                                                                     >  2:2209106/1‑150 (MQ=21)

                   aaaGGAATTAAATATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAggg                                                                                                                                    <  1:2439162/150‑1 (MQ=255)

                   aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATGGAATCGGTTGTGGCGAgtg                                                                                                                                    >  2:3463611/1‑148 (MQ=18)

                      ggAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACAGAATAC‑TTTTTATGA‑ATTCGGAGCGTTGGGTGCGAgggggg                                                                                                                                 >  1:876282/1‑150 (MQ=17)

                       gAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGTGAggggggg                                                                                                                                <  1:1284621/150‑1 (MQ=255)

                        aaGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTAC‑TTTGATAGT‑TATCGCAGCGGTTGGTGCGCgggggggt                                                                                                                               >  1:1458/1‑149 (MQ=17)

                        aaGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCAACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAgggggggg                                                                                                                               >  1:941895/1‑150 (MQ=255)

                         aGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGa                                                                                                                              <  2:87553/150‑1 (MQ=255)

                         aGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGa                                                                                                                              <  2:839881/150‑1 (MQ=255)

                         aGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGa                                                                                                                              <  1:362824/150‑1 (MQ=255)

                            aaaGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                           <  2:4595528/150‑1 (MQ=255)

                            aaaGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                           <  2:1410808/150‑1 (MQ=255)

                              aGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGa                                                                                                                         <  1:3019172/150‑1 (MQ=255)

                                     tGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                                                                                                  <  1:3371038/150‑1 (MQ=255)

                                      ggTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCAc                                                                                                                 <  1:868533/150‑1 (MQ=255)

                                        tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGt                                                                                                               <  2:4447133/150‑1 (MQ=255)

                                          cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTGTGA‑TATAGCCGCGTGTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc                                                                                                             >  2:1619744/1‑150 (MQ=255)

                                          cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCGTAC‑TTTTTTTGA‑TATGGCCACGTGGGGGGCGAGGGGGGGGGCTGGAAGCCACACGTcc                                                                                                             >  2:952061/1‑150 (MQ=18)

                                           gagtgtggggACTTGAACCCCCACGTCCGTAAGGACACTCACACCTGAAGCTAGCGCGTCTACCTATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCg                                                                                                            <  2:3558335/144‑1 (MQ=255)

                                               ggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                        <  2:2622904/150‑1 (MQ=255)

                                                  gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAac                                                                                                     <  1:2898661/150‑1 (MQ=255)

                                                  gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTATTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAac                                                                                                     >  2:322415/1‑150 (MQ=255)

                                                    gACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAacac                                                                                                   <  1:1602600/150‑1 (MQ=255)

                                                     aCTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTATACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                  <  1:902770/150‑1 (MQ=255)

                                                      cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                 <  1:4153467/150‑1 (MQ=255)

                                                      cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                 <  2:1986285/150‑1 (MQ=255)

                                                         gAACCCCCACGTCCGTATGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                              <  1:795099/150‑1 (MQ=255)

                                                         gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                              <  1:795234/150‑1 (MQ=255)

                                                            cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                           >  2:4497646/1‑150 (MQ=255)

                                                              cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                         <  2:3262748/150‑1 (MQ=255)

                                                                cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                                                       >  2:3643359/1‑150 (MQ=255)

                                                                cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTAC‑TTTAAAAGA‑TATCGCATCTGTTGGTGCGAGGGGGGGGACTTGAACCGCCTAGTGCGGAAGAACACAAACACCTGAAg                                                                                       >  2:3788074/1‑150 (MQ=21)

                                                                cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAAAGACAACTTAC‑TTTATTTGA‑TATCGACACGGTTGGTCCGAGGGGGGGGAGTTGAACCAACACGAACGGAAGAGCACAAACACCTGAAg                                                                                       >  2:3643390/1‑150 (MQ=18)

                                                                  cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                                     <  1:685547/150‑1 (MQ=255)

                                                                       gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                                <  1:4594977/150‑1 (MQ=255)

                                                                         aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                                                              >  2:4177717/1‑150 (MQ=255)

                                                                          aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCTCGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTc                                                                             >  2:1154556/1‑150 (MQ=255)

                                                                          aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTATAGATTATC‑CCTCGGTTGGGGCGAGGGGGGGGACTTTAACCCAAACGTCCGTAAGAACAACAACACCGGAAGCTAGagcgac                                                                             >  2:935372/1‑148 (MQ=21)

                                                                            gACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACATATTAC‑TTTTTTTGA‑TATAGCATCTTTTGGTGCGAGGGGGGTGACTTGAACCCCCACTACCGTAAGAAAACTAACACCTGAAGCTAGCGCGTCTa                                                                           >  2:489176/1‑150 (MQ=25)

                                                                                cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                       >  1:1440177/1‑150 (MQ=255)

                                                                                 tAACACCTGAAGCTAGCGCGTCTACCTATTCCGCCACCTTCTCACAGTCATCTTAG‑TTTTTTTGA‑TATAGCTTCGTTTGGTGCGTGGGGGGGGAATTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                      <  1:61378/150‑1 (MQ=25)

                                                                                 tAAAACCTGCAGCTATCGAGTCTACCTATTCCGCCACGATAGCACAGTGATCTTAC‑TTTTTTTGA‑TGTAGCCACTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                      <  2:3115788/150‑1 (MQ=18)

                                                                                 tAAAACCTGAAGATAGCGCGTCTACCAATTCCGCCACCTTCGCACATTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                      <  2:602285/150‑1 (MQ=255)

                                                                                     acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                  >  1:2551117/1‑150 (MQ=255)

                                                                                       cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACGTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                 >  2:3848165/1‑150 (MQ=255)

                                                                                       cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGGTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                >  1:1733440/1‑150 (MQ=255)

                                                                                        tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                               >  1:2112061/1‑150 (MQ=255)

                                                                                          aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCcccc                                                             >  1:52616/1‑150 (MQ=255)

                                                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          >  1:3121466/1‑150 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                         <  1:2884733/150‑1 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                         >  1:1342481/1‑150 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                         >  1:1363225/1‑150 (MQ=255)

                                                                                              tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTATTTGA‑TATCGCATCGTTTGGTGCGAGGGGGGGGACTTGAACCCCAACGTACGTAAGAACACTAACACCTGAAGATAGCGACTCTACCAATTCCGCCACATTCg                                                         >  1:4516879/1‑150 (MQ=25)

                                                                                                 cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                      <  1:1514882/150‑1 (MQ=255)

                                                                                                    gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcatacc                                                   >  2:3570864/1‑145 (MQ=255)

                                                                                                    gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                   <  1:1164923/150‑1 (MQ=255)

                                                                                                         ccAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                              <  2:4345720/150‑1 (MQ=255)

                                                                                                         ccAATTCCGCCACCTACTCACATTCATCTTAC‑TTTTTTTGA‑TTTAGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                              <  2:1967989/150‑1 (MQ=18)

                                                                                                            aTTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTTA‑TATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa                                           <  2:455471/150‑1 (MQ=255)

                                                                                                             gtCCGCCACCTTCGCACAGTCATCTTAC‑ATTTTTTGA‑TCTCGACTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat                                          <  2:3493638/149‑1 (MQ=255)

                                                                                                              tCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                         <  1:231172/150‑1 (MQ=255)

                                                                                                                cGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaaaaac                                       >  1:969366/1‑146 (MQ=255)

                                                                                                                cGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                       >  1:1033710/1‑150 (MQ=255)

                                                                                                                 gCCACGTTCTAACAGTCATCTAAC‑TTTTTTTGA‑TATCTCCTCGTTTGGTGCGTGGGGGGGGTCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                      <  1:1360941/150‑1 (MQ=21)

                                                                                                                 gCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                      <  2:1452266/150‑1 (MQ=255)

                                                                                                                 gCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCaa                                     <  1:3517452/150‑1 (MQ=255)

                                                                                                                      cTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                 >  2:1175022/1‑150 (MQ=255)

                                                                                                                       ttCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                                >  2:1035609/1‑150 (MQ=255)

                                                                                                                       ttCGAACATTCATCTTAC‑ATTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                                <  2:1326322/150‑1 (MQ=255)

                                                                                                                           cacaGTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGACCGTAAGAACACTAACACCTGAAGCTAGCGAGTCGACCAATTCCGCCACCTTCGCCAAGGGCCAGAAATAGCAAAGtcgaaat                            >  1:3550089/1‑144 (MQ=18)

                                                                                                                              aGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGATCACTATCAACGTGGTTATTgg                         >  2:1087541/1‑150 (MQ=255)

                                                                                                                               gTCATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                        >  1:2274640/1‑150 (MQ=255)

                                                                                                                                tCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTTGTGCGAGGGGGGGGACTTGAACCCCCACGTACGTAATAACACTAACACCTTAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAAACAGCGAACAATATAAACGTGGTTTTTGGTg                       >  2:1710625/1‑150 (MQ=18)

                                                                                                                                tCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                       >  2:1710628/1‑150 (MQ=255)

                                                                                                                                 cATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                      <  2:1139514/150‑1 (MQ=255)

                                                                                                                                 cATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                      <  2:204999/150‑1 (MQ=255)

                                                                                                                                 cATCTTAC‑TTTTTTTGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGAGCGAGCACTATCAAAGTGGTTGTAGGTGc                      >  2:4274253/1‑150 (MQ=18)

                                                                                                                                  aTCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                     <  2:1259542/150‑1 (MQ=255)

                                                                                                                                    cTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAACAGAGAGCTCTATCAAAGTGTTGATTGGTGCGAg                   >  2:2271023/1‑150 (MQ=17)

                                                                                                                                        c‑TTTTTTTGA‑TATCTCCACTTTTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg               <  1:842869/150‑1 (MQ=255)

                                                                                                                                           ttttttGA‑TATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg             <  1:3316283/150‑1 (MQ=255)

                                                                                                                                             ttttGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa           <  1:4568190/150‑1 (MQ=255)

                                                                                                                                             tttgGA‑CATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa           <  2:221493/150‑1 (MQ=255)

                                                                                                                                                tGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt        >  1:2047520/1‑150 (MQ=255)

                                                                                                                                                tGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGAGCGAGCAATATCAACGTGGTTGTTGGTGCGAGGGGGGGGAAtt        >  1:4109793/1‑150 (MQ=255)

                                                                                                                                                 gA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg       >  1:533450/1‑150 (MQ=255)

                                                                                                                                                     atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACAGAGAGAAATATCAAAGAGGTGTTTGGAGCGAGGCGGGGTCCTTGaaa    >  2:583719/1‑149 (MQ=17)

AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTTGA‑TATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCC  >  CP009273/4595859‑4596157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`