Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,596,108 T→A 5.6% intergenic (‑5/+24) leuP ← / ← leuQ tRNA‑Leu/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,596,1080TA 5.6% 469.0 / 4.1 163intergenic (‑5/+24)leuP/leuQtRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (88/62);  new base A (10/2);  total (99/64)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGC  >  CP009273/4595959‑4596257
                                                                                                                                                     |                                                                                                                                                     
gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                                                                                                       >  2:3570870/1‑150 (MQ=255)
    aCCAATTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATATCGACTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                                                                                                                   <  2:909018/150‑1 (MQ=255)
     ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                                                                                  >  2:3083544/1‑150 (MQ=255)
     ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                                                                                  <  2:4345720/150‑1 (MQ=255)
     ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                                                                                  <  2:4345715/150‑1 (MQ=255)
        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTTATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa                                                                                                                                               <  2:455471/150‑1 (MQ=255)
         gtCCGCCACCTTCGCACAGTCATCTTACATTTTTTGATCTCGACTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat                                                                                                                                              <  2:3493638/149‑1 (MQ=255)
            cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaaaaac                                                                                                                                           >  1:969366/1‑146 (MQ=255)
            cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                                                                                                                           >  1:1033710/1‑150 (MQ=255)
             gCCACGTTCTAACAGTCATCTAACTTTTTTTGATATCTCCTCGTTTGGTGCGTGGGGGGGGTCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                                                                                                                          <  1:1360941/150‑1 (MQ=21)
             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                                                                                                                          <  2:1452266/150‑1 (MQ=255)
             gCCACCTTCGAACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa                                                                                                                                          <  2:3776881/150‑1 (MQ=255)
                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                                                                                     >  2:1175022/1‑150 (MQ=255)
                   ttCGAACATTCATCTTACATTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                                                                                                                                    <  2:1326322/150‑1 (MQ=255)
                      gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttt                                                                                                                                 >  1:1135844/1‑150 (MQ=255)
                       cacaGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGACCGTAAGAACACTAACACCTGAAGCTAGCGAGTCGACCAATTCCGCCACCTTCGCCAAGGGCCAGAAATAGCAAAGtcgaaat                                                                                                                                >  1:3550089/1‑144 (MQ=18)
                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGATCACTATCAACGTGGTTATTgg                                                                                                                             >  2:1087541/1‑150 (MQ=255)
                           gTCATCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                                                                                                            >  1:2274640/1‑150 (MQ=255)
                           gTCATCTTACTTTTTTTGATATAGCCACGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                                                                                                            <  2:3839449/150‑1 (MQ=255)
                            tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                                                                                           >  2:1710628/1‑150 (MQ=255)
                            tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGAGAGAGCACTATCAAAGTGGTGTTTGGTg                                                                                                                           >  2:1710619/1‑150 (MQ=21)
                             cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                                                                                                                          <  2:204999/150‑1 (MQ=255)
                             cATCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGAGCGAGCACTATCAAAGTGGTTGTAGGTGc                                                                                                                          >  2:4274253/1‑150 (MQ=18)
                              aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                                                                                                         <  2:1259542/150‑1 (MQ=255)
                              aTCTTACTTTTTTTGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                                                                                                         >  1:1354671/1‑150 (MQ=255)
                                  tACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggg                                                                                                                     >  1:1168250/1‑150 (MQ=255)
                                    cTTTTTTTGATATCTCCACTTTTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                                                                                   <  1:842869/150‑1 (MQ=255)
                                      ttttttGATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                                                                                                                 <  1:3316283/150‑1 (MQ=255)
                                        ttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa                                                                                                               <  1:4568216/150‑1 (MQ=255)
                                        tttgGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa                                                                                                               <  1:4568138/150‑1 (MQ=255)
                                        tttgGACATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa                                                                                                               <  2:221493/150‑1 (MQ=255)
                                            gATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg                                                                                                           >  1:533450/1‑150 (MQ=255)
                                            gATATAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg                                                                                                           <  1:1409779/150‑1 (MQ=255)
                                               atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGAGATCACTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAc                                                                                                        >  2:583936/1‑150 (MQ=255)
                                                   ccTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTTAAccccc                                                                                                    >  1:3738730/1‑150 (MQ=255)
                                                   ccTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc                                                                                                    >  1:1601680/1‑150 (MQ=255)
                                                   ccTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc                                                                                                    >  1:4626598/1‑150 (MQ=255)
                                                    cTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                                                                                   >  2:3468624/1‑150 (MQ=255)
                                                     tCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCAc                                                                                                  <  1:322415/150‑1 (MQ=255)
                                                     tCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCAc                                                                                                  <  1:3749687/150‑1 (MQ=255)
                                                         ttGGTGCGTGGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTTGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCTACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc                                                                                              <  1:3892722/150‑1 (MQ=25)
                                                             tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGAGAGCAATATCAACGAGTTTGTTGGTGCGAGGGGGGGGACTTGAAACCCAACGTCCGTaa                                                                                          >  2:2158306/1‑150 (MQ=21)
                                                               cgcggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCCCAGTGCGAGAAATATCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                        <  1:2209104/147‑1 (MQ=255)
                                                               cGAGGGGGGGGACTTGAACCCCCTCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATTTCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                        <  1:2209106/150‑1 (MQ=255)
                                                               cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                        >  2:4506198/1‑150 (MQ=255)
                                                               cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGGAAgg                                                                                        >  1:2502725/1‑150 (MQ=255)
                                                                gAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCACACGTCCGTCAGGa                                                                                       >  2:2061436/1‑150 (MQ=255)
                                                                gAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAAAGATCGATAAATATCAACGTGTTTATTGGTGCGAGGGGGGTGACTTGAACCCCAACGTCCGTAAGGa                                                                                       >  2:1132882/1‑150 (MQ=18)
                                                                 aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                      >  2:2384370/1‑150 (MQ=255)
                                                                   gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                                                    >  1:4541815/1‑150 (MQ=255)
                                                                       gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                                                                >  1:396423/1‑150 (MQ=255)
                                                                            tcgcaCCCCCACGTCCGTAAGAACACTACCACCTTAACCTAGCGCGTCTACCAATTCCGCCACCTCCGCCCAGTGCGAGCAATATAAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                                           <  2:941895/146‑1 (MQ=25)
                                                                              caaCCCCCACGTCCCTAAGAACACTATCACATGAAGATATATATTCTACACATTACCCAACCTTAGCCCACTGCTAGCAATATCAAATTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa                                                                         <  2:451135/149‑1 (MQ=12)
                                                                                  ccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                     >  1:1546159/1‑150 (MQ=255)
                                                                                      aCGTCCGTAAGTACCCTCACCCCTTAAGCTAGCTCTTCTACCAATTCCGCCCCCTTCGCCCAGTGCGAGCTATATCAACTTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                 <  2:2300904/150‑1 (MQ=21)
                                                                                         tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGCGAGCACTATCAACGAGGTTTTTCGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                              >  2:1465244/1‑150 (MQ=255)
                                                                                          ccGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                             >  1:3233391/1‑150 (MQ=255)
                                                                                           cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                            >  2:2447623/1‑150 (MQ=255)
                                                                                             tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                          >  2:537350/1‑150 (MQ=255)
                                                                                              aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                         >  2:711232/1‑150 (MQ=255)
                                                                                              aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                         >  2:2600211/1‑150 (MQ=255)
                                                                                               aGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                        >  2:4582497/1‑150 (MQ=255)
                                                                                               aGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                        >  2:4555859/1‑150 (MQ=255)
                                                                                                gAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                       >  1:2698661/1‑150 (MQ=255)
                                                                                                gAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                       >  1:2698713/1‑150 (MQ=255)
                                                                                                  acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                     >  2:4439177/1‑150 (MQ=255)
                                                                                                  acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                     >  2:2219349/1‑150 (MQ=255)
                                                                                                   cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTTCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                    <  2:1033710/150‑1 (MQ=255)
                                                                                                      tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                 >  1:2181139/1‑150 (MQ=255)
                                                                                                       aaCACCTGCAGCTAGCGCGTCTACCAATTCCGACACCTTCGCCCATTTCGAGCAATAAAAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                <  2:4007698/150‑1 (MQ=21)
                                                                                                        acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                               >  2:4165567/1‑150 (MQ=255)
                                                                                                            cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                           >  1:4264170/1‑150 (MQ=255)
                                                                                                             tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                          >  1:2611706/1‑150 (MQ=255)
                                                                                                               aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                        >  2:1500407/1‑150 (MQ=255)
                                                                                                                 gCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa                                      >  2:3465088/1‑150 (MQ=255)
                                                                                                                  cTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAc                                     >  1:2985384/1‑150 (MQ=255)
                                                                                                                   tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAcc                                    >  1:1897782/1‑150 (MQ=255)
                                                                                                                     gcgcGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                  <  2:1135844/150‑1 (MQ=255)
                                                                                                                           cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                            >  1:3091614/1‑150 (MQ=255)
                                                                                                                             tccACTTCCGCCACATCCGCCCAGTGCTAGCAACATCAAAGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                          <  2:1458/149‑1 (MQ=18)
                                                                                                                             aCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                          >  1:4019885/1‑150 (MQ=255)
                                                                                                                                   tCCGCCACCTTCGCCCAGTGCGAGCAATTTCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAg                    <  2:1342481/150‑1 (MQ=255)
                                                                                                                                    ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                   >  1:577946/1‑150 (MQ=255)
                                                                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                  >  1:1298813/1‑150 (MQ=255)
                                                                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                  >  1:1298848/1‑150 (MQ=255)
                                                                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                  >  1:3482589/1‑150 (MQ=255)
                                                                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                  >  1:1298640/1‑150 (MQ=255)
                                                                                                                                      gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                 >  2:4200358/1‑150 (MQ=255)
                                                                                                                                       ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAtt                >  1:4369664/1‑150 (MQ=255)
                                                                                                                                       ccACCGTCGCCCTGTTCTAGCAATTTCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAta                <  2:3129559/150‑2 (MQ=18)
                                                                                                                                        cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAtag               >  2:2084070/1‑148 (MQ=255)
                                                                                                                                           cTTCGCCCAGTGCTTGAAATATCAACGTGGTTTTTGGTGCGAGGGGGGGTACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTa            <  1:489176/150‑1 (MQ=21)
                                                                                                                                            ttCGCCCAGTGCTAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTAc           <  1:2724394/150‑1 (MQ=255)
                                                                                                                                            ttCGCCCAGTGCTAGAAATATCATCGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTAc           <  1:1710619/150‑1 (MQ=255)
                                                                                                                                              cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCa         >  1:1792402/1‑150 (MQ=255)
                                                                                                                                               gCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc        >  1:837549/1‑150 (MQ=255)
                                                                                                                                               gCCAAGTGCTATCAATCTATAAGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCAc        <  1:3463611/150‑1 (MQ=18)
                                                                                                                                                   aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAg    <  2:2990312/150‑1 (MQ=255)
                                                                                                                                                   aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAg    >  2:1765298/1‑150 (MQ=255)
                                                                                                                                                   aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATAGCTACCACGGAg    >  2:1764920/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGC  >  CP009273/4595959‑4596257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑