BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,617,009	G→T	12.6%	A427A (GCG→GCT)	radA →	DNA repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,617,009	0	G	T	12.6%	879.5 / 64.9	259	A427A (GCG→GCT)	radA	DNA repair protein
Reads supporting (aligned to +/- strand): ref base G (102/124); new base T (17/16); total (119/140)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 264 total aligned reads displayed.

CTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACT  >  CP009273/4616860‑4617158

ctgctgGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCg                                                                                                                                                       >  2:3492661/1‑150 (MQ=255)

 tgctgGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGa                                                                                                                                                      >  1:1012892/1‑150 (MQ=255)

   ctgGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGAtt                                                                                                                                                    <  2:461593/150‑1 (MQ=255)

    tgGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTg                                                                                                                                                   >  2:2802539/1‑150 (MQ=255)

     ggCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGt                                                                                                                                                  >  2:2853967/1‑150 (MQ=255)

     ggCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGt                                                                                                                                                  <  2:1103034/150‑1 (MQ=255)

        gATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTcc                                                                                                                                               <  2:1922161/150‑1 (MQ=255)

        gATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTcc                                                                                                                                               <  2:3146166/150‑1 (MQ=255)

         aTGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCcg                                                                                                                                              <  1:1114517/150‑1 (MQ=255)

         aTGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCcg                                                                                                                                              >  1:1907503/1‑150 (MQ=255)

          tGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCcgg                                                                                                                                             <  2:1057206/150‑1 (MQ=255)

             tttCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCcggcgg                                                                                                                                          >  2:3253354/1‑150 (MQ=255)

              ttCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCggcggc                                                                                                                                         <  2:64148/150‑1 (MQ=255)

               tCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCt                                                                                                                                        <  1:3528662/150‑1 (MQ=255)

                       gcgcGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGcc                                                                                                                                <  2:1326441/150‑1 (MQ=255)

                         gcgACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGa                                                                                                                              >  1:1735444/1‑150 (MQ=255)

                          cgACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGaa                                                                                                                             >  1:4055727/1‑150 (MQ=255)

                              aGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGaaaaaa                                                                                                                         >  1:3892756/1‑150 (MQ=255)

                                 ccGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAgcg                                                                                                                      <  2:3714827/150‑1 (MQ=255)

                                  cGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAgcgc                                                                                                                     >  2:1240473/1‑150 (MQ=255)

                                  cGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAgcgc                                                                                                                     >  2:3498820/1‑150 (MQ=255)

                                   gCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGcc                                                                                                                    <  1:1450496/150‑1 (MQ=255)

                                    cTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCg                                                                                                                   <  2:2539106/150‑1 (MQ=255)

                                     tGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCgg                                                                                                                  >  2:2870214/1‑150 (MQ=255)

                                      gCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGa                                                                                                                 <  1:3854340/150‑1 (MQ=255)

                                       ccGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGaa                                                                                                                <  1:3492661/150‑1 (MQ=255)

                                        cGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGTCGAAACACGGTTTTCGCCGGGCGAGTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAg                                                                                                               >  1:1693010/1‑150 (MQ=255)

                                        cGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAg                                                                                                               >  2:1997274/1‑150 (MQ=255)

                                         gCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAgg                                                                                                              <  2:3670077/150‑1 (MQ=255)

                                          cAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAggg                                                                                                             >  1:1837773/1‑150 (MQ=255)

                                           aGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGa                                                                                                            >  2:3769239/1‑150 (MQ=255)

                                           aGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGa                                                                                                            >  2:1362091/1‑150 (MQ=255)

                                            ggATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGAt                                                                                                           >  2:3373405/1‑150 (MQ=255)

                                            ggATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGAt                                                                                                           <  1:1066482/150‑1 (MQ=255)

                                            ggATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGAt                                                                                                           <  2:1496748/150‑1 (MQ=255)

                                            ggATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGAt                                                                                                           <  1:887076/150‑1 (MQ=255)

                                                cTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAg                                                                                                       >  2:2423266/1‑150 (MQ=255)

                                                  ggtggtGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGAt                                                                                                     >  2:2303151/1‑150 (MQ=255)

                                                   gtggtgTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGAtt                                                                                                    >  1:1621632/1‑150 (MQ=255)

                                                   gtggtgTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGAtt                                                                                                    <  2:165033/150‑1 (MQ=255)

                                                    tggtgTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGAttt                                                                                                   <  2:1528501/150‑1 (MQ=255)

                                                     ggtgTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGAtttt                                                                                                  >  1:2919234/1‑150 (MQ=255)

                                                         tttGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGc                                                                                              >  1:2696984/1‑150 (MQ=255)

                                                           tGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGt                                                                                            <  2:1484699/150‑1 (MQ=255)

                                                             gTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTa                                                                                          <  1:926185/150‑1 (MQ=255)

                                                               gAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaa                                                                                        >  1:3746488/1‑150 (MQ=255)

                                                                aaGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaaa                                                                                       <  1:2439158/150‑1 (MQ=255)

                                                                  gTCGGGCTGGCAGGGTAGATCCGCCCGGGTCCAAGCGGTCAGGAAAGAATGTCTTAAGCGGCGAAACACTGTTTTCGCCGGGCGATTGTTACGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaaaaa                                                                                     <  1:554352/150‑1 (MQ=255)

                                                                      ggCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAActct                                                                                 <  2:4510765/150‑1 (MQ=255)

                                                                       gCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACtctc                                                                                <  1:646222/150‑1 (MQ=255)

                                                                       gCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACtctc                                                                                <  2:489515/150‑1 (MQ=255)

                                                                        cTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTcc                                                                               >  2:3836435/1‑150 (MQ=255)

                                                                         tGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCg                                                                              >  2:3977057/1‑150 (MQ=255)

                                                                           gCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAc                                                                            >  1:2984507/1‑150 (MQ=255)

                                                                           gCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAc                                                                            <  2:2915722/150‑1 (MQ=255)

                                                                            cAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcg                                                                           >  2:4142449/1‑150 (MQ=255)

                                                                             aGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcgc                                                                          >  1:485568/1‑150 (MQ=255)

                                                                              ggggAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcgcg                                                                         <  1:711498/150‑1 (MQ=255)

                                                                                        gCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCTAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCgtgt                                                               >  1:4339174/1‑150 (MQ=255)

                                                                                        gCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCgtgt                                                               <  2:3018838/150‑1 (MQ=255)

                                                                                            ggTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcga                                                           >  2:940543/1‑150 (MQ=255)

                                                                                             gTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgac                                                          <  2:2459446/150‑1 (MQ=255)

                                                                                             gTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgac                                                          <  2:2459539/150‑1 (MQ=255)

                                                                                              tGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgacg                                                         <  1:3253141/150‑1 (MQ=255)

                                                                                              tGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgacg                                                         <  1:691773/150‑1 (MQ=255)

                                                                                                cccAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCgacgac                                                       <  1:2303151/150‑1 (MQ=255)

                                                                                                cccAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCgacgac                                                       <  1:4274508/150‑1 (MQ=255)

                                                                                                 ccAGCGGTCAGGAACTAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGTTTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACt                                                      <  1:4004759/150‑1 (MQ=255)

                                                                                                         cAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATga                                              >  2:851748/1‑150 (MQ=255)

                                                                                                             aaCGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGata                                          >  1:864281/1‑150 (MQ=255)

                                                                                                              aCGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGatat                                         >  2:1885661/1‑150 (MQ=255)

                                                                                                               cGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAGAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGAtata                                        <  1:4387463/150‑1 (MQ=255)

                                                                                                               cGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGAtata                                        <  2:4045476/150‑1 (MQ=255)

                                                                                                               cGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGAtata                                        <  1:3374043/150‑1 (MQ=255)

                                                                                                                 aaTCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACg                                      >  2:3916238/1‑150 (MQ=255)

                                                                                                                 aaTCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACg                                      >  1:2754158/1‑150 (MQ=255)

                                                                                                                  aTCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACgg                                     <  1:2149018/150‑1 (MQ=255)

                                                                                                                   tctcTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACgga                                    >  1:1784390/1‑150 (MQ=255)

                                                                                                                    ctctGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACggag                                   >  1:1440153/1‑150 (MQ=255)

                                                                                                                     tctGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACggagg                                  <  1:4074255/150‑1 (MQ=255)

                                                                                                                      ctGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACggaggg                                 <  1:1823251/150‑1 (MQ=255)

                                                                                                                       tGAAGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACggaggga                                <  2:2346186/150‑1 (MQ=255)

                                                                                                                       tGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACggaggga                                <  2:2090264/150‑1 (MQ=255)

                                                                                                                         aaGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga                              >  2:1671812/1‑150 (MQ=255)

                                                                                                                         aaGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTACGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga                              >  1:1658780/1‑150 (MQ=255)

                                                                                                                          aGCGGCGAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGat                             <  2:2723038/150‑1 (MQ=255)

                                                                                                                             ggcgAAACACGGTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATg                          <  2:1314144/150‑1 (MQ=255)

                                                                                                                             ggcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATg                          >  2:2495424/1‑150 (MQ=255)

                                                                                                                             ggcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATg                          >  2:13722/1‑150 (MQ=255)

                                                                                                                               cgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATgtc                        >  2:3877012/1‑150 (MQ=255)

                                                                                                                                    cacGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCAt                   <  1:1099136/150‑1 (MQ=255)

                                                                                                                                       ggTTTTCGCCGGGCTATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTg                <  1:394157/150‑1 (MQ=255)

                                                                                                                                        gTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGa               <  1:2802629/150‑1 (MQ=255)

                                                                                                                                        gTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGa               >  1:3332582/1‑150 (MQ=255)

                                                                                                                                        gTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGa               <  2:1464194/150‑1 (MQ=255)

                                                                                                                                             cGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTAcc          >  2:2595614/1‑150 (MQ=255)

                                                                                                                                              gCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCt         >  2:2002367/1‑150 (MQ=255)

                                                                                                                                              gCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGA‑TTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTTTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTg        <  1:2480937/150‑1 (MQ=255)

                                                                                                                                                cGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGa       >  2:3252878/1‑150 (MQ=255)

                                                                                                                                                cGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGa       <  1:1398051/150‑1 (MQ=255)

CTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACT  >  CP009273/4616860‑4617158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`