Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,624,764 C→A 35.8% E122* (GAA→TAA)  rob ← right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,624,7640CA35.8% 419.3 / 244.0 251E122* (GAA→TAA) robright oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base C (101/60);  new base A (43/46);  total (144/107)
Fisher's exact test for biased strand distribution p-value = 3.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

CGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAAT  >  CP009273/4624615‑4624911
                                                                                                                                                     |                                                                                                                                                   
cgcgTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTc                                                                                                                                                     >  2:1043363/1‑150 (MQ=255)
  cgTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCAc                                                                                                                                                   >  1:1669013/1‑150 (MQ=255)
   gTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCAcc                                                                                                                                                  >  1:2063127/1‑150 (MQ=255)
        cGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGc                                                                                                                                             <  2:423692/150‑1 (MQ=255)
         gagaAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCg                                                                                                                                            >  1:675646/1‑150 (MQ=255)
              aaTCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAgcg                                                                                                                                       <  1:1886838/150‑1 (MQ=255)
              aaTCGTGCCAGAACTGATAACGCAATTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAgcg                                                                                                                                       <  1:3229896/150‑1 (MQ=255)
                 cGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAgcggcg                                                                                                                                    <  2:1865857/150‑1 (MQ=255)
                 cGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAgcggcg                                                                                                                                    <  2:4196912/150‑1 (MQ=255)
                  gTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGcggcgg                                                                                                                                   >  1:2263335/1‑150 (MQ=255)
                     ccAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCggcgggcg                                                                                                                                >  1:1590296/1‑150 (MQ=255)
                     ccAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCggcgggcg                                                                                                                                >  2:4585554/1‑150 (MQ=255)
                      cAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGa                                                                                                                               >  1:2590206/1‑150 (MQ=255)
                       aGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGaa                                                                                                                              >  2:236711/1‑150 (MQ=255)
                        gAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAAt                                                                                                                             <  1:1364709/150‑1 (MQ=255)
                           cTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATAcc                                                                                                                          <  2:1677603/150‑1 (MQ=255)
                            tGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCa                                                                                                                         >  2:401167/1‑150 (MQ=255)
                                aaCGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAgg                                                                                                                     <  2:107332/150‑1 (MQ=255)
                                    cATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCt                                                                                                                 >  1:2641021/1‑150 (MQ=255)
                                     aTTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTc                                                                                                                >  1:4214793/1‑150 (MQ=255)
                                         cATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCAtt                                                                                                            <  1:2333611/150‑1 (MQ=255)
                                         cATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCAtt                                                                                                            >  2:2599546/1‑150 (MQ=255)
                                           tGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCa                                                                                                          <  1:1928173/150‑1 (MQ=255)
                                           tGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCa                                                                                                          <  1:3845843/150‑1 (MQ=255)
                                             gCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAgg                                                                                                        <  1:3302451/150‑1 (MQ=255)
                                              cGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGga                                                                                                       <  2:4640178/150‑1 (MQ=255)
                                              cGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGga                                                                                                       >  2:4469414/1‑150 (MQ=255)
                                               ggAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGgag                                                                                                      <  2:2022242/150‑1 (MQ=255)
                                                gAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGgaga                                                                                                     <  2:2670700/150‑1 (MQ=255)
                                                 aaaTCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGaa                                                                                                    >  2:2317933/1‑150 (MQ=255)
                                                 aaaTCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGaa                                                                                                    >  1:1975666/1‑150 (MQ=255)
                                                   aTCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTCTCTTCCAGGGTGACCAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAAcg                                                                                                  >  1:921092/1‑150 (MQ=255)
                                                   aTCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAAcg                                                                                                  <  2:659266/150‑1 (MQ=255)
                                                   aTCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAAcg                                                                                                  >  1:521107/1‑150 (MQ=255)
                                                   aTCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAAcg                                                                                                  <  1:3412936/150‑1 (MQ=255)
                                                      agagATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAAcggcg                                                                                               >  2:1470278/1‑150 (MQ=255)
                                                          aTTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTaa                                                                                           >  1:811471/1‑150 (MQ=255)
                                                                 ccAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAg                                                                                    <  1:4114483/150‑1 (MQ=255)
                                                                  cAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGTGGTAAAGTGCAgg                                                                                   <  1:3056252/150‑1 (MQ=255)
                                                                     cGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGt                                                                                >  1:2940674/1‑150 (MQ=255)
                                                                     cGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGt                                                                                >  1:2940075/1‑150 (MQ=255)
                                                                     cGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGt                                                                                <  1:1665812/150‑1 (MQ=255)
                                                                      gAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTc                                                                               >  2:1389119/1‑150 (MQ=255)
                                                                      gAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTc                                                                               <  1:3382495/150‑1 (MQ=255)
                                                                      gAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTc                                                                               <  1:751558/150‑1 (MQ=255)
                                                                      gAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTc                                                                               <  1:727601/150‑1 (MQ=255)
                                                                      gAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTAACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTc                                                                               >  1:3635136/1‑150 (MQ=255)
                                                                       aaCAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCt                                                                              >  1:1162313/1‑150 (MQ=255)
                                                                        aCAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTg                                                                             <  2:4044544/150‑1 (MQ=255)
                                                                         cAGGAGTAGCTCTGGGTAACACCAATCCGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTgg                                                                            <  2:1169340/150‑1 (MQ=255)
                                                                          aGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTggg                                                                           >  2:3733485/1‑150 (MQ=255)
                                                                          aGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTggg                                                                           >  1:3881709/1‑150 (MQ=255)
                                                                          aGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTggg                                                                           <  2:1519952/150‑1 (MQ=255)
                                                                          aGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTggg                                                                           >  1:2372756/1‑150 (MQ=255)
                                                                            gAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCa                                                                         <  2:2306073/150‑1 (MQ=255)
                                                                              gTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCaaa                                                                       >  1:4545464/1‑150 (MQ=255)
                                                                               tAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAAc                                                                      >  2:827523/1‑150 (MQ=255)
                                                                                  ctctGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGc                                                                   >  2:268110/1‑150 (MQ=255)
                                                                                   tctGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGct                                                                  >  2:3011377/1‑150 (MQ=255)
                                                                                    ctGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGctt                                                                 <  1:1314466/150‑1 (MQ=255)
                                                                                     tGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGcttc                                                                >  1:1758083/1‑150 (MQ=255)
                                                                                     tGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGcttc                                                                >  1:2433124/1‑150 (MQ=255)
                                                                                     tGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATCTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACAGcttc                                                                >  2:1410998/1‑150 (MQ=255)
                                                                                      gggTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGcttct                                                               <  2:4604635/150‑1 (MQ=255)
                                                                                       ggTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAACTTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGcttctt                                                              <  2:4130032/150‑1 (MQ=255)
                                                                                       ggTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGcttctt                                                              <  1:2099715/150‑1 (MQ=255)
                                                                                       ggTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGcttctt                                                              >  1:2749143/1‑150 (MQ=255)
                                                                                          aaCACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGaa                                                           >  2:3542466/1‑150 (MQ=255)
                                                                                             acCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATgc                                                        <  2:597023/150‑1 (MQ=255)
                                                                                             acCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATgc                                                        <  2:1704359/150‑1 (MQ=255)
                                                                                             acCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATgc                                                        <  2:2916796/150‑1 (MQ=255)
                                                                                              ccAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATgcg                                                       >  1:3794084/1‑150 (MQ=255)
                                                                                               cAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATgcgc                                                      >  1:4174839/1‑150 (MQ=255)
                                                                                                  tCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCggg                                                   <  2:494394/150‑1 (MQ=255)
                                                                                                    aGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTa                                                 >  1:2524794/1‑150 (MQ=255)
                                                                                                    aGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTa                                                 <  2:4339734/150‑1 (MQ=255)
                                                                                                         cgTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGt                                            >  2:2304212/1‑150 (MQ=255)
                                                                                                          gTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTc                                           <  2:2358814/150‑1 (MQ=255)
                                                                                                           tATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCt                                          >  2:1217319/1‑150 (MQ=255)
                                                                                                            aTCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTg                                         <  2:3279475/150‑1 (MQ=255)
                                                                                                             tCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGt                                        >  2:2231685/1‑150 (MQ=255)
                                                                                                                 ccAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTgag                                    >  2:255857/1‑150 (MQ=255)
                                                                                                                   aGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGagag                                  >  1:2109810/1‑150 (MQ=255)
                                                                                                                     ggTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTc                                <  2:2159505/150‑1 (MQ=255)
                                                                                                                           aaaTTTGTGCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCg                          >  1:1008056/1‑150 (MQ=255)
                                                                                                                            aaTTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCgg                         >  1:4271005/1‑150 (MQ=255)
                                                                                                                            aaTTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCACCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAAATGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCgg                         >  2:3379937/1‑150 (MQ=255)
                                                                                                                                 gtgCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTg                    <  2:1812065/150‑1 (MQ=255)
                                                                                                                                  tgCTCTGGCATAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGc                   <  2:3630324/150‑1 (MQ=255)
                                                                                                                                     tctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAgc                >  1:2019466/1‑150 (MQ=255)
                                                                                                                                      ctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAgcg               >  2:2481632/1‑150 (MQ=255)
                                                                                                                                      ctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAgcg               >  2:74536/1‑150 (MQ=255)
                                                                                                                                      ctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAgcg               >  2:74491/1‑150 (MQ=255)
                                                                                                                                       tGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAgcgc              >  1:247143/1‑150 (MQ=255)
                                                                                                                                        ggCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGcgcg             >  2:3503808/1‑150 (MQ=255)
                                                                                                                                        ggCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGcgcg             >  2:2321231/1‑150 (MQ=255)
                                                                                                                                          cATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGAt           <  1:1732893/150‑1 (MQ=255)
                                                                                                                                           aTAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATg          >  1:290454/1‑150 (MQ=255)
                                                                                                                                           aTAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATg          >  1:1376042/1‑150 (MQ=255)
                                                                                                                                           aTAGTGAATTAACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTATGTTGAGAGTCGAAGCGGTATTGCAGCGCGATg          >  1:3949323/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAAT  >  CP009273/4624615‑4624911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑