Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 437,371 G→T 11.8% D123Y (GAT→TAT)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273437,3710GT11.8% 969.5 / 68.7 271D123Y (GAT→TAT) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base G (111/128);  new base T (16/16);  total (127/144)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 280 total aligned reads displayed.

TCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTC  >  CP009273/437222‑437520
                                                                                                                                                     |                                                                                                                                                     
tCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTg                                                                                                                                                       <  1:1794001/150‑1 (MQ=255)
  caccaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGAt                                                                                                                                                     >  2:1062416/1‑150 (MQ=255)
   accaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTAtg                                                                                                                                                    <  1:1795117/150‑1 (MQ=255)
   accaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGAtg                                                                                                                                                    >  1:4187051/1‑150 (MQ=255)
   accaTATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGAtg                                                                                                                                                    <  2:705882/150‑1 (MQ=255)
      atatTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTgg                                                                                                                                                 >  1:473393/1‑150 (MQ=255)
        atTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGaa                                                                                                                                               >  1:1326557/1‑150 (MQ=255)
          tctcGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGCACg                                                                                                                                             <  2:4519622/150‑1 (MQ=255)
            tcgaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGtt                                                                                                                                           <  1:2648893/150‑1 (MQ=255)
            tcgaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTAAGCTCGATTGATGTGGAACGtt                                                                                                                                           >  1:2428881/1‑150 (MQ=255)
             cgaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTa                                                                                                                                          <  2:1078607/150‑1 (MQ=255)
             cgaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTa                                                                                                                                          <  1:4487707/150‑1 (MQ=255)
             cgaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTa                                                                                                                                          <  2:2731302/150‑1 (MQ=255)
             cgaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTa                                                                                                                                          <  2:2571253/150‑1 (MQ=255)
              gaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTAc                                                                                                                                         >  2:4171998/1‑150 (MQ=255)
              gaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTAc                                                                                                                                         >  2:4071497/1‑150 (MQ=255)
              gaagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTAc                                                                                                                                         <  2:3834004/150‑1 (MQ=255)
               aagtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGACCGTTACg                                                                                                                                        >  2:2488979/1‑150 (MQ=255)
                agtcgaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGt                                                                                                                                       >  1:2020760/1‑150 (MQ=255)
                    gaagACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTcggc                                                                                                                                   >  2:3267298/1‑150 (MQ=255)
                      agACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTcggcgg                                                                                                                                 <  1:4076363/150‑1 (MQ=255)
                      agACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTcggcgg                                                                                                                                 <  2:2881820/150‑1 (MQ=255)
                          gTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGt                                                                                                                             <  1:634886/150‑1 (MQ=255)
                            gCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGttt                                                                                                                           >  1:987374/1‑150 (MQ=255)
                            gCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGttt                                                                                                                           <  1:404940/150‑1 (MQ=255)
                             ccGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTa                                                                                                                          >  1:2132828/1‑150 (MQ=255)
                             ccGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTa                                                                                                                          <  2:1391788/150‑1 (MQ=255)
                              cGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTaa                                                                                                                         >  2:2173019/1‑150 (MQ=255)
                              cGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTaa                                                                                                                         <  2:415209/150‑1 (MQ=255)
                               gTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGGTTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTaaa                                                                                                                        >  1:2844883/1‑150 (MQ=255)
                               gTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTaaa                                                                                                                        <  1:346307/150‑1 (MQ=255)
                               gTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTaaa                                                                                                                        <  1:361118/150‑1 (MQ=255)
                                  tACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCa                                                                                                                     <  2:1232991/150‑1 (MQ=255)
                                    ccGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGc                                                                                                                   >  1:347433/1‑150 (MQ=255)
                                      gACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCat                                                                                                                 >  2:3848306/1‑150 (MQ=255)
                                      gACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCat                                                                                                                 <  1:1145857/150‑1 (MQ=255)
                                       aCATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCata                                                                                                                <  2:3953529/150‑1 (MQ=255)
                                        cATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCatat                                                                                                               >  1:2125203/1‑150 (MQ=255)
                                         aTGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATAtt                                                                                                              <  2:1752382/150‑1 (MQ=255)
                                          tGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTg                                                                                                             <  1:2981974/150‑1 (MQ=255)
                                             aCGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAAt                                                                                                          >  2:3352191/1‑150 (MQ=255)
                                             aCGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAAt                                                                                                          <  2:231766/150‑1 (MQ=255)
                                               gATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATcc                                                                                                        >  2:2694864/1‑150 (MQ=255)
                                                atatTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCcg                                                                                                       >  2:3809440/1‑150 (MQ=255)
                                                  atTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCcgcg                                                                                                     >  1:3199995/1‑150 (MQ=255)
                                                        gagaAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGcgtgcgtg                                                                                               >  2:3162784/1‑150 (MQ=255)
                                                         agaAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGa                                                                                              >  1:2701998/1‑150 (MQ=255)
                                                          gaAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGaa                                                                                             <  1:2905440/150‑1 (MQ=255)
                                                            aaGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGc                                                                                           >  1:1710405/1‑150 (MQ=255)
                                                              gCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTg                                                                                         <  1:686004/150‑1 (MQ=255)
                                                               cGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGa                                                                                        >  2:4526551/1‑150 (MQ=255)
                                                                gTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGAc                                                                                       >  2:3948638/1‑150 (MQ=255)
                                                                  tGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCa                                                                                     <  1:4505196/150‑1 (MQ=255)
                                                                    gTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAAt                                                                                   >  1:1044617/1‑150 (MQ=255)
                                                                     ttCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATc                                                                                  <  1:3785053/150‑1 (MQ=255)
                                                                        aGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCgg                                                                               >  2:4319728/1‑150 (MQ=255)
                                                                          tATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGAt                                                                             <  2:1923469/150‑1 (MQ=255)
                                                                           aTCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGAtg                                                                            >  1:3131357/1‑150 (MQ=255)
                                                                           aTCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCCTATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGAtg                                                                            >  2:392381/1‑150 (MQ=255)
                                                                            tCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGAtgt                                                                           <  1:4158624/150‑1 (MQ=255)
                                                                            tCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGAtgt                                                                           <  2:3233597/150‑1 (MQ=255)
                                                                               cgATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGAc                                                                        >  2:1466146/1‑150 (MQ=255)
                                                                               cgATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGAc                                                                        >  1:3922659/1‑150 (MQ=255)
                                                                                    aGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGAGGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTcc                                                                   >  1:2991632/1‑150 (MQ=255)
                                                                                     gCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCa                                                                  >  1:193690/1‑150 (MQ=255)
                                                                                        ggAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCt                                                               >  1:3239829/1‑150 (MQ=255)
                                                                                          aaGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCtgg                                                             <  1:2710922/150‑1 (MQ=255)
                                                                                           aGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCtgga                                                            >  2:1738375/1‑150 (MQ=255)
                                                                                             gCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCtggaag                                                          <  1:1874419/150‑1 (MQ=255)
                                                                                              cAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCtggaagt                                                         >  2:1116942/1‑150 (MQ=255)
                                                                                               aaaaCCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCtggaagtg                                                        >  2:2469833/1‑150 (MQ=255)
                                                                                                 aaCCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTTGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCtggaagtgga                                                      >  1:2319934/1‑150 (MQ=255)
                                                                                                 aaCCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCtggaagtgga                                                      >  2:3624956/1‑150 (MQ=255)
                                                                                                    cTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGa                                                   >  1:90707/1‑150 (MQ=255)
                                                                                                          cGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCg                                             <  2:1146253/150‑1 (MQ=255)
                                                                                                           gTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGt                                            >  2:3705732/1‑150 (MQ=255)
                                                                                                             aCGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGtct                                          <  1:1614826/150‑1 (MQ=255)
                                                                                                             aCGCGTGAAGCGCCGGGGCAAACATGATTTTAGCTCGATTGAGGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGtct                                          <  2:3605966/150‑1 (MQ=255)
                                                                                                                   gAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCctgct                                    >  2:790753/1‑150 (MQ=255)
                                                                                                                    aaGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCctgctg                                   >  2:3533143/1‑150 (MQ=255)
                                                                                                                     aGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGa                                  >  2:2335214/1‑150 (MQ=255)
                                                                                                                     aGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGa                                  >  2:4385160/1‑150 (MQ=255)
                                                                                                                       cgcCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGAtt                                >  1:80023/1‑150 (MQ=255)
                                                                                                                        gcCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTa                               <  2:2492785/150‑1 (MQ=255)
                                                                                                                         ccGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTaa                              <  1:4610255/150‑1 (MQ=255)
                                                                                                                          cGTGGCAAACATGATTTTAGCTCGATTTATGTTGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTaaa                             <  2:2401826/150‑1 (MQ=255)
                                                                                                                            tGGCAAACATGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAgg                           >  2:2125920/1‑150 (MQ=255)
                                                                                                                               cAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCg                        <  2:4638016/150‑1 (MQ=255)
                                                                                                                               cAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCg                        >  2:1268926/1‑150 (MQ=255)
                                                                                                                                 aaCATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCt                      >  1:3046901/1‑150 (MQ=255)
                                                                                                                                  aCATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCCATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTa                     <  2:1555386/150‑1 (MQ=255)
                                                                                                                                     tGATTTTAGCTCGATTTATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGa                  >  2:2767355/1‑150 (MQ=255)
                                                                                                                                      gATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGaa                 <  1:443308/150‑1 (MQ=255)
                                                                                                                                        ttttAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAgg               >  1:3679742/1‑150 (MQ=255)
                                                                                                                                           tAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTAt            <  2:1828777/150‑1 (MQ=255)
                                                                                                                                            aGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTAtt           >  2:4251164/1‑150 (MQ=255)
                                                                                                                                              cTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTgg         <  2:401431/150‑1 (MQ=255)
                                                                                                                                                cGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCg       <  2:2246699/150‑1 (MQ=255)
                                                                                                                                                  aTTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGt     <  2:1326542/150‑1 (MQ=255)
                                                                                                                                                     gATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTc  >  2:2936914/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCACCATATTCTCGAAGTCGAAGACGTGCCGTTTACCGACATGCACGATATTTTCGAGAAAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTC  >  CP009273/437222‑437520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑