Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 55,590 G→T 100% T507K (ACG→AAG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927355,5900GT98.9% 941.2 / 1.1 280T507K (ACG→AAG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (108/169);  total (109/171)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 285 total aligned reads displayed.

TTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTG  >  CP009273/55443‑55735
                                                                                                                                                   |                                                                                                                                                 
ttAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGATACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCttt                                                                                                                                                 <  1:266556/150‑1 (MQ=255)
   gCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTgg                                                                                                                                              <  1:623455/150‑1 (MQ=255)
     ggAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGt                                                                                                                                            <  2:3458890/150‑1 (MQ=255)
     ggAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGt                                                                                                                                            <  2:3563037/150‑1 (MQ=255)
      gAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGta                                                                                                                                           <  2:3773060/150‑1 (MQ=255)
       aGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGACCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtat                                                                                                                                          >  1:3914829/1‑150 (MQ=255)
       aGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtat                                                                                                                                          >  1:3029359/1‑150 (MQ=255)
         gCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATaa                                                                                                                                        <  1:3331984/150‑1 (MQ=255)
         gCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATaa                                                                                                                                        <  1:1008510/150‑1 (MQ=255)
                ggTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAg                                                                                                                                 >  1:2230631/1‑150 (MQ=255)
                 gTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGc                                                                                                                                <  2:4301665/150‑1 (MQ=255)
                  tCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGcc                                                                                                                               <  1:2906336/150‑1 (MQ=255)
                    aaGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAg                                                                                                                             >  2:3708654/1‑150 (MQ=255)
                      gACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCa                                                                                                                           <  1:1814847/150‑1 (MQ=255)
                        ccgccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCAtt                                                                                                                         <  2:4540742/150‑1 (MQ=255)
                         cgccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCAttt                                                                                                                        >  1:2054703/1‑150 (MQ=255)
                         cgccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCAttt                                                                                                                        <  2:2118763/150‑1 (MQ=255)
                           ccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTcc                                                                                                                      >  2:1390426/1‑150 (MQ=255)
                           ccgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTcc                                                                                                                      <  1:447535/150‑1 (MQ=255)
                            cgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCa                                                                                                                     <  2:2961727/150‑1 (MQ=255)
                            cgTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCa                                                                                                                     <  2:3236809/150‑1 (MQ=255)
                             gTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                    <  2:4581347/150‑1 (MQ=255)
                             gTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                    >  1:2265138/1‑150 (MQ=255)
                             gTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                    <  1:2140660/150‑1 (MQ=255)
                             gTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                    >  1:686591/1‑150 (MQ=255)
                               aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCatat                                                                                                                  >  1:2479699/1‑150 (MQ=255)
                                 gTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATcg                                                                                                                <  2:1500197/150‑1 (MQ=255)
                                   ccGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATcgcg                                                                                                              <  1:669634/150‑1 (MQ=255)
                                   ccGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATcgcg                                                                                                              >  2:2699633/1‑150 (MQ=255)
                                   ccGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATcgcg                                                                                                              >  2:2787700/1‑150 (MQ=255)
                                    cGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGt                                                                                                             >  2:3491192/1‑150 (MQ=255)
                                      gTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTc                                                                                                           <  1:967052/150‑1 (MQ=255)
                                         ccGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCaaa                                                                                                        <  2:3029585/150‑1 (MQ=255)
                                          cGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAg                                                                                                       >  2:2566890/1‑150 (MQ=255)
                                                aGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtt                                                                                                 >  1:2313398/1‑150 (MQ=255)
                                                     agagTAGTCAGATTGCAGCAGAGACGAGTCGTANTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGcc                                                                                            <  1:192299/150‑1 (MQ=255)
                                                     agagTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGcc                                                                                            >  2:1138764/1‑150 (MQ=255)
                                                          agtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCaa                                                                                       <  1:1422634/150‑1 (MQ=255)
                                                           gtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAAc                                                                                      >  1:358373/1‑150 (MQ=255)
                                                           gtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAAc                                                                                      <  2:4256457/150‑1 (MQ=255)
                                                            tCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACt                                                                                     <  1:4292797/150‑1 (MQ=255)
                                                             cAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACtt                                                                                    >  2:2515721/1‑150 (MQ=255)
                                                             cAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACtt                                                                                    >  1:3510722/1‑150 (MQ=255)
                                                               gATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTg                                                                                  <  1:1086092/150‑1 (MQ=255)
                                                                  tGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAAt                                                                               <  1:1671196/150‑1 (MQ=255)
                                                                       cagAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCgg                                                                          <  1:1230237/150‑1 (MQ=255)
                                                                        agagACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGc                                                                         >  2:4170923/1‑150 (MQ=255)
                                                                         gagaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCa                                                                        >  2:1954216/1‑150 (MQ=255)
                                                                          agaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                                       <  2:309545/150‑1 (MQ=255)
                                                                          agaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                                       >  2:4107294/1‑150 (MQ=255)
                                                                           gaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAtt                                                                      <  1:2744423/150‑1 (MQ=255)
                                                                           gaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAtt                                                                      <  1:2744502/150‑1 (MQ=255)
                                                                              gAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcg                                                                   >  1:2601908/1‑150 (MQ=255)
                                                                              gAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcg                                                                   >  1:3145382/1‑150 (MQ=255)
                                                                                   gTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGtt                                                              >  1:104367/1‑150 (MQ=255)
                                                                                   gTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGtt                                                              <  2:4603054/150‑1 (MQ=255)
                                                                                     aGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTaa                                                            >  1:194407/1‑150 (MQ=255)
                                                                                      gTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAAc                                                           >  2:3919766/1‑150 (MQ=255)
                                                                                      gTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAAc                                                           <  2:2024547/150‑1 (MQ=255)
                                                                                       ttATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACg                                                          <  2:4440704/150‑1 (MQ=255)
                                                                                         ataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGa                                                        <  2:598263/150‑1 (MQ=255)
                                                                                          taGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAt                                                       <  1:1552389/150‑1 (MQ=255)
                                                                                            gATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTc                                                     <  2:1315398/150‑1 (MQ=255)
                                                                                            gATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTc                                                     <  2:4473034/150‑1 (MQ=255)
                                                                                              tGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGt                                                   <  1:1857547/150‑1 (MQ=255)
                                                                                                tCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTcc                                                 <  1:842212/150‑1 (MQ=255)
                                                                                                 cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                                                <  1:3751385/150‑1 (MQ=255)
                                                                                                  gCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAg                                               >  2:1259683/1‑150 (MQ=255)
                                                                                                       gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTgg                                          <  2:3542203/150‑1 (MQ=255)
                                                                                                        aTCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGt                                         >  1:2105525/1‑150 (MQ=255)
                                                                                                        aTCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGt                                         >  1:152239/1‑150 (MQ=255)
                                                                                                         tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTc                                        >  2:3057101/1‑150 (MQ=255)
                                                                                                         tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTc                                        <  1:4624794/150‑1 (MQ=255)
                                                                                                           gcgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgt                                      <  1:1645736/150‑1 (MQ=255)
                                                                                                            cgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtg                                     >  1:284126/1‑150 (MQ=255)
                                                                                                            cgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtg                                     >  2:2083070/1‑150 (MQ=255)
                                                                                                              aTACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGtt                                   <  1:2422962/150‑1 (MQ=255)
                                                                                                                aCGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc                                 <  1:4096777/150‑1 (MQ=255)
                                                                                                                 cGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCt                                >  1:760630/1‑150 (MQ=255)
                                                                                                                   gCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg                              >  1:189110/1‑150 (MQ=255)
                                                                                                                   gCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg                              <  2:1911787/150‑1 (MQ=255)
                                                                                                                   gCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg                              <  1:581524/150‑1 (MQ=255)
                                                                                                                      cGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt                           <  1:2518107/150‑1 (MQ=255)
                                                                                                                      cGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt                           <  1:4250113/150‑1 (MQ=255)
                                                                                                                         aCAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtat                        <  2:1380475/150‑1 (MQ=255)
                                                                                                                           aaaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAc                      >  2:4154354/1‑150 (MQ=255)
                                                                                                                            aaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAcc                     >  2:2322224/1‑150 (MQ=255)
                                                                                                                            aaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAcc                     <  1:378126/150‑1 (MQ=255)
                                                                                                                                 tGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCa                <  1:4435765/150‑1 (MQ=255)
                                                                                                                                 tGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCa                <  2:782236/150‑1 (MQ=255)
                                                                                                                                  gcgcgcgcGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCaa               <  2:1112784/150‑1 (MQ=255)
                                                                                                                                      gcgcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGAtt           >  2:4562418/1‑150 (MQ=255)
                                                                                                                                      gcgcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGAtt           <  2:1717516/150‑1 (MQ=255)
                                                                                                                                       cgcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTg          >  2:1685168/1‑150 (MQ=255)
                                                                                                                                         cgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGt        >  1:120883/1‑150 (MQ=255)
                                                                                                                                         cgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGt        >  1:4180050/1‑150 (MQ=255)
                                                                                                                                          ggTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGtt       >  1:4391256/1‑150 (MQ=255)
                                                                                                                                          ggTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGtt       >  2:3384152/1‑150 (MQ=255)
                                                                                                                                              ccAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCt   >  2:4337012/1‑150 (MQ=255)
                                                                                                                                               cAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTg  <  2:906568/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTG  >  CP009273/55443‑55735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑