BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	538,153	A→C	14.9%	E270D (GAA→GAC)	glxK →	glycerate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	538,153	0	A	C	14.9%	733.1 / 149.3	321	E270D (GAA→GAC)	glxK	glycerate kinase II
Reads supporting (aligned to +/- strand): ref base A (136/137); new base C (31/17); total (167/154)
Fisher's exact test for biased strand distribution p-value = 6.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 330 total aligned reads displayed.

AACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGAT  >  CP009273/538005‑538302

aaCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGaaa                                                                                                                                                      <  1:1166704/150‑1 (MQ=255)

aaCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGaaa                                                                                                                                                      <  1:2243035/150‑1 (MQ=255)

  cTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAAtt                                                                                                                                                    <  1:2321932/150‑1 (MQ=255)

   ttGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATtg                                                                                                                                                   <  2:1965527/150‑1 (MQ=255)

   ttGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCGGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATtg                                                                                                                                                   <  2:1645134/150‑1 (MQ=255)

    tGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATtgt                                                                                                                                                  <  2:672246/150‑1 (MQ=255)

     gAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATtgtg                                                                                                                                                 <  2:3627589/150‑1 (MQ=255)

       aCAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGtt                                                                                                                                               <  1:3596002/150‑1 (MQ=255)

        cAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTg                                                                                                                                              <  2:3344259/150‑1 (MQ=255)

        cAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTg                                                                                                                                              >  2:4534125/1‑150 (MQ=255)

           gggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGGAt                                                                                                                                           >  1:3126795/1‑150 (MQ=255)

           gggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAAt                                                                                                                                           <  2:1066099/150‑1 (MQ=255)

              cTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCg                                                                                                                                        >  2:1414585/1‑150 (MQ=255)

                     aTTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCACTGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATc                                                                                                                                 <  2:1075136/150‑1 (MQ=255)

                       tACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCtt                                                                                                                               <  2:341732/150‑1 (MQ=255)

                        aCGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTg                                                                                                                              <  2:2420065/150‑1 (MQ=255)

                        aCGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTg                                                                                                                              <  1:450074/150‑1 (MQ=255)

                         cGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTgc                                                                                                                             >  1:329962/1‑150 (MQ=255)

                         cGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTgc                                                                                                                             >  2:2556650/1‑150 (MQ=255)

                           cccGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTgcgc                                                                                                                           >  2:2418198/1‑150 (MQ=255)

                               tgtTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAAGTGTGTTGAATGCGGTCAATCTTGCgcaggc                                                                                                                       >  2:2725276/1‑150 (MQ=255)

                                 ttttACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcaggcag                                                                                                                     >  1:3439303/1‑150 (MQ=255)

                                  tttACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGt                                                                                                                    >  2:1796004/1‑150 (MQ=255)

                                    tACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGc                                                                                                                  >  2:2022917/1‑150 (MQ=255)

                                    tACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGc                                                                                                                  >  1:204579/1‑150 (MQ=255)

                                     acaccaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCa                                                                                                                 <  2:4243680/150‑1 (MQ=255)

                                       aacaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAgg                                                                                                               >  1:3438953/1‑150 (MQ=255)

                                           gCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGgcgc                                                                                                           >  1:3210249/1‑150 (MQ=255)

                                            cAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgca                                                                                                          >  1:365860/1‑150 (MQ=255)

                                            cAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgca                                                                                                          >  1:1774028/1‑150 (MQ=255)

                                                cTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCAc                                                                                                      >  2:862199/1‑150 (MQ=255)

                                                cTGAAATTAATGACTGCCAGATGGCGGGCGGCTGCGCTGCGGTCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCAc                                                                                                      >  2:48854/1‑150 (MQ=255)

                                                 tGAAATTAATGTCTGCCAGATGGCGGGCGGCGGNGCTGCGGGCNGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGTGCAGCACt                                                                                                     <  1:3081998/150‑1 (MQ=255)

                                                  gAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTg                                                                                                    >  1:4057766/1‑150 (MQ=255)

                                                  gAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTg                                                                                                    >  1:3217945/1‑150 (MQ=255)

                                                    aaTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGt                                                                                                  >  1:652540/1‑150 (MQ=255)

                                                     aTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTg                                                                                                 <  1:4365727/150‑1 (MQ=255)

                                                       tAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGAt                                                                                               <  2:4267602/150‑1 (MQ=255)

                                                       tAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGAt                                                                                               <  1:1401366/150‑1 (MQ=255)

                                                       tAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGAt                                                                                               >  2:2520544/1‑150 (MQ=255)

                                                        aaTGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGAtt                                                                                              >  1:4547639/1‑150 (MQ=255)

                                                         aTGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTa                                                                                             <  1:887214/150‑1 (MQ=255)

                                                          tGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAc                                                                                            <  1:2515749/150‑1 (MQ=255)

                                                          tGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAc                                                                                            <  1:2103476/150‑1 (MQ=255)

                                                           gTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAcc                                                                                           >  2:4175604/1‑150 (MQ=255)

                                                           gTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAcc                                                                                           >  2:3297327/1‑150 (MQ=255)

                                                           gTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAcc                                                                                           <  2:583344/150‑1 (MQ=255)

                                                           gTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAcc                                                                                           <  2:1521149/150‑1 (MQ=255)

                                                               gCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCgggg                                                                                       >  1:2443206/1‑150 (MQ=255)

                                                                ccAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTAAATGCGGATATTAAACAGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGa                                                                                      >  2:2177106/1‑150 (MQ=255)

                                                                  aGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAg                                                                                    >  2:1663458/1‑150 (MQ=255)

                                                                   gATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAgg                                                                                   >  1:3726900/1‑150 (MQ=255)

                                                                        cgggcggCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCa                                                                              >  1:1627111/1‑150 (MQ=255)

                                                                         gggcggCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCAt                                                                             <  2:2318858/150‑1 (MQ=255)

                                                                         gggcggCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCAt                                                                             >  2:2437960/1‑150 (MQ=255)

                                                                         gggcggCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCAt                                                                             >  1:2186172/1‑150 (MQ=255)

                                                                          ggcggcggcGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATc                                                                            <  1:1948238/150‑1 (MQ=255)

                                                                                ggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCg                                                                      >  1:270703/1‑150 (MQ=255)

                                                                                ggcgCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCg                                                                      >  2:3073012/1‑150 (MQ=255)

                                                                                  cgcTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCa                                                                    >  1:3855133/1‑150 (MQ=255)

                                                                                    cTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCaaa                                                                  <  1:296049/150‑1 (MQ=255)

                                                                                     tGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAAc                                                                 <  2:1227856/150‑1 (MQ=255)

                                                                                       cgggcggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACgg                                                               <  1:1103897/150‑1 (MQ=255)

                                                                                          gcggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAg                                                            <  1:4256847/150‑1 (MQ=255)

                                                                                           cggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAgg                                                           >  2:3706005/1‑150 (MQ=255)

                                                                                            ggTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGc                                                          <  1:1233855/150‑1 (MQ=255)

                                                                                             gTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCg                                                         >  2:2947934/1‑150 (MQ=255)

                                                                                                 gggTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTaa                                                     >  1:783326/1‑150 (MQ=255)

                                                                                                  ggTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTaaa                                                    <  1:408720/150‑1 (MQ=255)

                                                                                                   gTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAg                                                   >  2:3480983/1‑150 (MQ=255)

                                                                                                       tGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGcgcc                                               <  2:444666/150‑1 (MQ=255)

                                                                                                           gcggcggTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTg                                           <  1:1362313/150‑1 (MQ=255)

                                                                                                           gcggcggTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTg                                           <  1:1457018/150‑1 (MQ=255)

                                                                                                                ggTATTTCTCAATGCGGATATTAAACCGGGCATTGAAAGTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGgtgt                                      <  1:2971464/150‑1 (MQ=255)

                                                                                                                 gTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGtgtg                                     >  1:2866637/1‑150 (MQ=255)

                                                                                                                 gTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGtgtg                                     >  2:843581/1‑150 (MQ=255)

                                                                                                                       ctcAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGTCTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCg                               >  2:3877596/1‑150 (MQ=255)

                                                                                                                       ctcAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCg                               >  1:1546507/1‑150 (MQ=255)

                                                                                                                       ctcAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCg                               >  2:2122/1‑150 (MQ=255)

                                                                                                                        tcAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCgg                              >  1:2838955/1‑150 (MQ=255)

                                                                                                                         cAATGCGGATATTAAACCGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGt                             >  1:545078/1‑150 (MQ=255)

                                                                                                                         cAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGt                             >  2:4401258/1‑150 (MQ=255)

                                                                                                                          aaTGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTg                            >  2:2856566/1‑150 (MQ=255)

                                                                                                                           aTGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTgg                           >  1:4194705/1‑150 (MQ=255)

                                                                                                                            tGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGc                          <  1:510159/150‑1 (MQ=255)

                                                                                                                                gATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAg                      <  2:53425/150‑1 (MQ=255)

                                                                                                                                  tatTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCa                    >  2:4023957/1‑150 (MQ=255)

                                                                                                                                  tatTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCa                    <  1:2774003/150‑1 (MQ=255)

                                                                                                                                   atTAAACCGGGCATTGAAATGGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCATGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAg                   >  2:2234303/1‑150 (MQ=255)

                                                                                                                                      aaaCCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGttt                >  2:4343617/1‑150 (MQ=255)

                                                                                                                                      aaaCCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGttt                >  2:4532844/1‑150 (MQ=255)

                                                                                                                                         ccGGGCATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAAt             <  1:2432965/150‑1 (MQ=255)

                                                                                                                                           gggCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGt           <  1:3686121/150‑1 (MQ=255)

                                                                                                                                            ggCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTa          <  1:1000208/150‑1 (MQ=255)

                                                                                                                                             gCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTAc         <  2:2078294/150‑1 (MQ=255)

                                                                                                                                              cATTGACATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTAcc        >  2:4335529/1‑150 (MQ=255)

                                                                                                                                               aTTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCg       <  2:3260034/150‑1 (MQ=255)

                                                                                                                                                  gAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTg    >  1:1307187/1‑150 (MQ=255)

                                                                                                                                                  gAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTg    <  2:3787284/150‑1 (MQ=255)

                                                                                                                                                    aaTTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGAt  >  2:2615452/1‑150 (MQ=255)

AACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGAT  >  CP009273/538005‑538302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`