Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 546,491 G→T 100% V199V (GTG→GTT)  ybcF → putative carbonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273546,4910GT98.2% 822.0 / 4.7 244V199V (GTG→GTT) ybcFputative carbonate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (110/129);  total (111/133)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 251 total aligned reads displayed.

TTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC  >  CP009273/546344‑546637
                                                                                                                                                   |                                                                                                                                                  
tttGCGACGGGTGGTGGCGTCTCCGGAACCGGGTAAAATTCTCGACAGCGAAGCATTCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCTGCGGTGTGACTGTGTCTGATGACGGTGCAGGGAGTGATGCAGttat                                                                                                                                                  <  1:1604082/150‑1 (MQ=255)
 ttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                 <  2:286023/150‑1 (MQ=255)
 ttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                 <  2:3206603/150‑1 (MQ=255)
 ttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                 <  2:3567283/150‑1 (MQ=255)
       cGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATaaa                                                                                                                                           <  1:2829234/150‑1 (MQ=255)
        gggTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGTAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAATCAGTTATTGATAAAg                                                                                                                                          <  1:2018157/150‑1 (MQ=255)
        gggTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAg                                                                                                                                          <  1:3953538/150‑1 (MQ=255)
         ggtggtGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGa                                                                                                                                         >  1:1192227/1‑150 (MQ=255)
         ggtggtGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGa                                                                                                                                         >  2:1186257/1‑150 (MQ=255)
         ggtggtGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGa                                                                                                                                         <  1:3062253/150‑1 (MQ=255)
         ggtggtGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGa                                                                                                                                         >  1:1285954/1‑150 (MQ=255)
             gtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtctc                                                                                                                                     <  1:404301/150‑1 (MQ=255)
              tggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcg                                                                                                                                    <  1:4095502/150‑1 (MQ=255)
               ggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgc                                                                                                                                   <  2:3237023/150‑1 (MQ=255)
                gCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTcgcc                                                                                                                                  <  1:2687961/150‑1 (MQ=255)
                  gTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgccgc                                                                                                                                >  1:3626293/1‑150 (MQ=255)
                  gTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgccgc                                                                                                                                >  1:2293437/1‑150 (MQ=255)
                   tctcCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCt                                                                                                                               <  1:2192195/150‑1 (MQ=255)
                          aaCCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGc                                                                                                                        >  2:2649141/1‑150 (MQ=255)
                          aaCCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGc                                                                                                                        <  2:4260777/150‑1 (MQ=255)
                            ccGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTc                                                                                                                      <  1:970266/150‑1 (MQ=255)
                             cgcgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCg                                                                                                                     >  2:2850876/1‑150 (MQ=255)
                               cgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGcc                                                                                                                   >  1:2925485/1‑150 (MQ=255)
                               cgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGcc                                                                                                                   >  1:1655868/1‑150 (MQ=255)
                               cgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGcc                                                                                                                   <  1:3506379/150‑1 (MQ=255)
                                gTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCg                                                                                                                  >  2:1174703/1‑150 (MQ=255)
                                   aaaTTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGc                                                                                                               >  1:2677105/1‑150 (MQ=255)
                                         tcGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTa                                                                                                         <  1:1747272/150‑1 (MQ=255)
                                           gACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAAt                                                                                                       <  1:1988468/150‑1 (MQ=255)
                                                cGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGa                                                                                                  >  2:3422243/1‑150 (MQ=255)
                                                cGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGa                                                                                                  >  2:3760682/1‑150 (MQ=255)
                                                  aaGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATg                                                                                                >  2:3888168/1‑150 (MQ=255)
                                                  aaGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATg                                                                                                >  2:2096842/1‑150 (MQ=255)
                                                  aaGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATg                                                                                                >  2:2096601/1‑150 (MQ=255)
                                                       aTCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTg                                                                                           <  2:2356869/150‑1 (MQ=255)
                                                         cGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                         <  2:3325962/150‑1 (MQ=255)
                                                         cGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                         >  2:2715118/1‑150 (MQ=255)
                                                           aGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGa                                                                                       <  2:846253/150‑1 (MQ=255)
                                                            gttgttGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAt                                                                                      <  1:4408584/150‑1 (MQ=255)
                                                                  gCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAc                                                                                <  1:4264965/150‑1 (MQ=255)
                                                                   cTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAcc                                                                               <  1:2113770/150‑1 (MQ=255)
                                                                     cAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGa                                                                             >  2:3124234/1‑150 (MQ=255)
                                                                      aaaGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGAt                                                                            >  2:3720986/1‑150 (MQ=255)
                                                                      aaaGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGAt                                                                            <  1:3609850/150‑1 (MQ=255)
                                                                        agagGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGc                                                                          <  1:4040635/150‑1 (MQ=255)
                                                                        agagGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGc                                                                          >  1:1385941/1‑150 (MQ=255)
                                                                          agGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTg                                                                        <  1:1712999/150‑1 (MQ=255)
                                                                          agGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTg                                                                        >  1:4490008/1‑150 (MQ=255)
                                                                                aTGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCgg                                                                  >  1:3788863/1‑150 (MQ=255)
                                                                                 tgtgGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGt                                                                 <  1:237896/150‑1 (MQ=255)
                                                                                    ggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtata                                                              >  1:4612159/1‑150 (MQ=255)
                                                                                     gtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTatat                                                             <  1:2878846/150‑1 (MQ=255)
                                                                                         tttGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGaaa                                                         >  1:2163851/1‑150 (MQ=255)
                                                                                           tGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAAc                                                       <  1:314851/150‑1 (MQ=255)
                                                                                            gCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTTATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACt                                                      <  2:184246/150‑1 (MQ=255)
                                                                                             cAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTg                                                     >  2:4409845/1‑150 (MQ=255)
                                                                                              aGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgg                                                    <  1:397576/150‑1 (MQ=255)
                                                                                               gTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTggg                                                   <  2:2265416/150‑1 (MQ=255)
                                                                                               gTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTggg                                                   <  1:1684062/150‑1 (MQ=255)
                                                                                                tGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg                                                  >  2:2374173/1‑150 (MQ=255)
                                                                                                tGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg                                                  <  2:2523219/150‑1 (MQ=255)
                                                                                                tGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg                                                  <  1:3291358/150‑1 (MQ=255)
                                                                                                 ggcggcggcGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGa                                                 >  1:2534205/1‑150 (MQ=255)
                                                                                                 ggcggcggcGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGa                                                 <  2:3540678/150‑1 (MQ=255)
                                                                                                   cggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAc                                               >  2:1121141/1‑150 (MQ=255)
                                                                                                   cggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAc                                               >  2:4562670/1‑150 (MQ=255)
                                                                                                   cggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAc                                               >  1:652507/1‑150 (MQ=255)
                                                                                                    ggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcg                                              >  2:1509435/1‑150 (MQ=255)
                                                                                                     gcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgc                                             <  2:990430/150‑1 (MQ=255)
                                                                                                         cggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCgca                                         <  1:3798294/150‑1 (MQ=255)
                                                                                                             gtgCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCaa                                     >  1:3477717/1‑150 (MQ=255)
                                                                                                              tgCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAAc                                    >  2:225682/1‑150 (MQ=255)
                                                                                                                ccTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGt                                  >  2:3160309/1‑150 (MQ=255)
                                                                                                                 cTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTg                                 <  2:3277084/150‑1 (MQ=255)
                                                                                                                 cTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTg                                 <  2:1949161/150‑1 (MQ=255)
                                                                                                                     gacggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCAt                             <  2:2776601/150‑1 (MQ=255)
                                                                                                                       cggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTc                           <  2:3868761/150‑1 (MQ=255)
                                                                                                                       cggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTc                           <  1:1824660/150‑1 (MQ=255)
                                                                                                                        ggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCg                          >  2:756061/1‑150 (MQ=255)
                                                                                                                          atgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGcc                        >  2:2812642/1‑150 (MQ=255)
                                                                                                                            gacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAt                      <  1:723514/150‑1 (MQ=255)
                                                                                                                              cggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGc                    <  1:4105376/150‑1 (MQ=255)
                                                                                                                                 aGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCcac                 <  1:3377736/150‑1 (MQ=255)
                                                                                                                                    aGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACAcc              <  1:3679243/150‑1 (MQ=255)
                                                                                                                                     gggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCg             >  2:2466090/1‑150 (MQ=255)
                                                                                                                                      ggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCgg            <  2:1687504/150‑1 (MQ=255)
                                                                                                                                      ggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCgg            <  2:1285954/150‑1 (MQ=255)
                                                                                                                                        aGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAt          <  1:2613820/150‑1 (MQ=255)
                                                                                                                                        aGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAt          <  1:1711696/150‑1 (MQ=255)
                                                                                                                                        aGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAt          <  1:3895116/150‑1 (MQ=255)
                                                                                                                                        aGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAt          <  1:4170793/150‑1 (MQ=255)
                                                                                                                                         gTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg         >  2:4101347/1‑150 (MQ=255)
                                                                                                                                         gTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg         >  1:167369/1‑150 (MQ=255)
                                                                                                                                         gTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg         <  1:3157559/150‑1 (MQ=255)
                                                                                                                                         gTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATg         >  1:1470312/1‑150 (MQ=255)
                                                                                                                                          tGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGa        <  1:204337/150‑1 (MQ=255)
                                                                                                                                          tGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGa        >  2:3066889/1‑150 (MQ=255)
                                                                                                                                              gCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTa    <  2:3429203/150‑1 (MQ=255)
                                                                                                                                               cAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCTGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAg   >  2:3369623/1‑150 (MQ=255)
                                                                                                                                               cAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAg   >  2:1555453/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC  >  CP009273/546344‑546637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑