Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,592 G→T 89.2% D814Y (GAC→TAC)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,5920GT89.2% 604.3 / 73.8 229D814Y (GAC→TAC) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (9/16);  new base T (80/123);  total (90/139)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 231 total aligned reads displayed.

CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557445‑557741
                                                                                                                                                   |                                                                                                                                                     
 cGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                                    <  2:3196309/150‑1 (MQ=255)
  gACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACgg                                                                                                                                                   <  2:2906311/150‑1 (MQ=255)
   aCGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACggg                                                                                                                                                  <  2:2240298/150‑1 (MQ=255)
   aCGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACggg                                                                                                                                                  <  2:3361229/150‑1 (MQ=255)
    cGCAAGGCGCGTTGGTTCGTGTCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGc                                                                                                                                                 <  1:393791/150‑1 (MQ=255)
    cGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGc                                                                                                                                                 <  2:80813/150‑1 (MQ=255)
    cGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGc                                                                                                                                                 <  1:4108278/150‑1 (MQ=255)
     gCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCa                                                                                                                                                >  2:1325803/1‑150 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGCTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  1:2582504/150‑1 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  2:2480205/150‑1 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  1:2767670/150‑1 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  1:2210523/150‑1 (MQ=255)
       aaGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAg                                                                                                                                              >  2:3692370/1‑150 (MQ=255)
          gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGttt                                                                                                                                           <  2:2541360/150‑1 (MQ=255)
              gTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttattt                                                                                                                                       <  2:3756633/150‑1 (MQ=255)
               ttGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGtttattta                                                                                                                                      <  1:492016/150‑1 (MQ=255)
               atGGTTCTTGCCAATTTTGATACCCGCATAGGCTTGCGGGCTCTCATTAACGTTACCCATGGCGGAAAACAGGTGCCGTTAGTATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGTGTGATACCGGGAAAGtttatttc                                                                                                                                      >  2:4627123/2‑149 (MQ=255)
                tGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTaa                                                                                                                                     >  1:492856/1‑150 (MQ=255)
                    tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtgg                                                                                                                                 <  2:411970/150‑1 (MQ=255)
                     cGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggt                                                                                                                                <  1:4073130/150‑1 (MQ=255)
                      gTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggtg                                                                                                                               <  1:2138173/150‑1 (MQ=255)
                       tGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTgc                                                                                                                              <  2:4257909/150‑1 (MQ=255)
                          cAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGTCGGAAAACCGGTGCCGTTTTGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                           <  2:634400/150‑1 (MQ=255)
                          cAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                           >  1:3462221/1‑150 (MQ=255)
                            aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCAt                                                                                                                         >  1:1446054/1‑150 (MQ=255)
                              tttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTg                                                                                                                       <  1:2606573/150‑1 (MQ=255)
                               ttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGt                                                                                                                      <  1:3329739/150‑1 (MQ=255)
                               ttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGt                                                                                                                      >  1:750969/1‑150 (MQ=255)
                                tGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTc                                                                                                                     >  2:1041133/1‑150 (MQ=255)
                                  aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTg                                                                                                                   >  1:2922543/1‑150 (MQ=255)
                                  aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACAAGTATGGTGGGTGATTACGGGCAAGTTTATATAAGTGGTGCGCCATTGTCTg                                                                                                                   >  1:614703/1‑150 (MQ=255)
                                    aCCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGt                                                                                                                 <  2:2391904/150‑1 (MQ=255)
                                     cccGCATAGGCGTGCGGGCGCTCATTACCGTGACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTg                                                                                                                <  2:4612263/150‑1 (MQ=255)
                                            aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACt                                                                                                         <  2:4434639/150‑1 (MQ=255)
                                             ggCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTg                                                                                                        <  2:3201501/150‑1 (MQ=255)
                                             ggCGTGCGGGCGCTCATTAACGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTAACGGAAAAACCAGTATGGTGGGTGATTACGGGCAAGGGTATTTAAGTGGTGCGCCCTTGTCGGGTGAATTACTg                                                                                                        >  1:2018677/1‑150 (MQ=255)
                                              gCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTgg                                                                                                       <  1:1702254/150‑1 (MQ=255)
                                                gTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGtt                                                                                                     >  1:4600/1‑150 (MQ=255)
                                                 tGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTc                                                                                                    >  2:948049/1‑150 (MQ=255)
                                                  gCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCa                                                                                                   <  2:2849300/150‑1 (MQ=255)
                                                       cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTggg                                                                                              >  2:1930608/1‑150 (MQ=255)
                                                       cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTggg                                                                                              >  2:131488/1‑150 (MQ=255)
                                                       cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTggg                                                                                              >  1:1924329/1‑150 (MQ=255)
                                                        gcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                             <  2:1619737/150‑1 (MQ=255)
                                                          tCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGgag                                                                                           >  1:1251335/1‑150 (MQ=255)
                                                           cATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGgaga                                                                                          <  1:1058021/150‑1 (MQ=255)
                                                            aTTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGAc                                                                                         >  2:3999524/1‑150 (MQ=255)
                                                                ccGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcg                                                                                     <  1:3327293/150‑1 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    <  2:2934812/150‑1 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    >  1:1791293/1‑150 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    <  2:2817623/150‑1 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    <  2:2677112/150‑1 (MQ=255)
                                                                  gTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGcgcg                                                                                   >  1:4373804/1‑150 (MQ=255)
                                                                     aCCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAc                                                                                >  1:2543245/1‑150 (MQ=255)
                                                                     aCCCAGGGCGGAAAACCGGTGCCGATTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAc                                                                                >  1:2330353/1‑150 (MQ=255)
                                                                         agtgCGGAAAAACGGTGCCGTTTGGATCACTGGTACGGTAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGTGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGTGAGACGGCGCGATCTCAc                                                                            <  2:1529263/147‑1 (MQ=255)
                                                                         aGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAc                                                                            <  1:985209/150‑1 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGTAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    <  1:1103669/150‑1 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    >  1:2061238/1‑150 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    <  1:1492047/150‑1 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    <  1:3861038/150‑1 (MQ=255)
                                                                                  aaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTg                                                                   <  1:4638875/150‑1 (MQ=255)
                                                                                     cGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTGATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTgcgc                                                                >  2:4317517/1‑150 (MQ=255)
                                                                                         gCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTa                                                            <  2:1120315/150‑1 (MQ=255)
                                                                                            gTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGt                                                         <  1:4354204/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       <  1:4312385/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       <  1:3567932/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       >  1:3836689/1‑150 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       <  2:3481740/150‑1 (MQ=255)
                                                                                               tGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAtt                                                      <  1:522976/150‑1 (MQ=255)
                                                                                                ggATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTg                                                     <  2:1067154/150‑1 (MQ=255)
                                                                                                 gATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGc                                                    <  1:4487667/150‑1 (MQ=255)
                                                                                                  aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                                   >  1:1229249/1‑150 (MQ=255)
                                                                                                   tCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCg                                                  <  1:1643046/150‑1 (MQ=255)
                                                                                                   tCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCg                                                  <  2:1347763/150‑1 (MQ=255)
                                                                                                    cACTGGTACGGGAAAACAGTACCGGACTAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGa                                                 <  1:1626057/150‑1 (MQ=255)
                                                                                                    cACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGa                                                 >  1:4521087/1‑150 (MQ=255)
                                                                                                     aCTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGaa                                                <  2:3422806/150‑1 (MQ=255)
                                                                                                      cTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAg                                               >  2:4331802/1‑150 (MQ=255)
                                                                                                        ggTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                             <  2:2078818/150‑1 (MQ=255)
                                                                                                         gTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCaa                                            >  1:2663593/1‑150 (MQ=255)
                                                                                                           aCGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAg                                          <  1:3692370/150‑1 (MQ=255)
                                                                                                               gAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                      >  2:2758183/1‑150 (MQ=255)
                                                                                                                 aaaCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTAAAGTGGTGCGCCATTGTCTGGTGAATTACGGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACa                                    >  1:3756930/1‑150 (MQ=255)
                                                                                                                     aGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCaa                                <  2:4261606/150‑1 (MQ=255)
                                                                                                                       tACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGc                              <  1:306214/150‑1 (MQ=255)
                                                                                                                           ggAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTc                          <  1:2050146/150‑1 (MQ=255)
                                                                                                                            gAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCa                         <  2:1516681/150‑1 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                       >  2:2757778/1‑150 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                       >  1:4228410/1‑150 (MQ=255)
                                                                                                                               tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTg                      >  2:1119132/1‑150 (MQ=255)
                                                                                                                                aaCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                     <  1:3953251/150‑1 (MQ=255)
                                                                                                                                aaCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                     <  1:3953337/150‑1 (MQ=255)
                                                                                                                                    aGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAtt                 <  1:815828/150‑1 (MQ=255)
                                                                                                                                      tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc               <  2:561544/150‑1 (MQ=255)
                                                                                                                                      tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGATCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc               >  2:1979527/1‑150 (MQ=255)
                                                                                                                                      tATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc               >  1:152289/1‑150 (MQ=255)
                                                                                                                                         ggtgggtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGc            <  2:2100983/150‑1 (MQ=255)
                                                                                                                                            gggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGt         <  2:3611365/150‑1 (MQ=255)
                                                                                                                                              gtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGttt       <  2:2675829/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557445‑557741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑