Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 596,812 G→T 15.6% M881I (ATG→ATT)  cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273596,8120GT15.6% 880.6 / 94.6 272M881I (ATG→ATT) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base G (126/103);  new base T (22/21);  total (148/124)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

GACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGCTTTAT  >  CP009273/596663‑596961
                                                                                                                                                     |                                                                                                                                                     
gACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAATCTCATGGTGCCGATGACGTtgatg                                                                                                                                                       >  1:3195538/1‑150 (MQ=255)
 aCATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtgatga                                                                                                                                                      <  1:2389327/150‑1 (MQ=255)
   aTGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGAtt                                                                                                                                                    >  1:2754008/1‑150 (MQ=255)
   aTGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGAtt                                                                                                                                                    <  2:2708780/150‑1 (MQ=255)
   aTGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGAtt                                                                                                                                                    <  1:2275936/150‑1 (MQ=255)
    tGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGAttatta                                                                                                                                                   <  1:4584779/150‑1 (MQ=255)
     ggTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATtattat                                                                                                                                                  <  1:4358770/150‑1 (MQ=255)
     ggTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTAt                                                                                                                                                  >  2:4462087/1‑150 (MQ=255)
     ggTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTAt                                                                                                                                                  >  1:1332637/1‑150 (MQ=255)
      gtgtCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATc                                                                                                                                                 >  1:3467757/1‑150 (MQ=255)
         tCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTc                                                                                                                                              <  1:321128/150‑1 (MQ=255)
            gtggtTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTg                                                                                                                                           >  2:2665765/1‑150 (MQ=255)
             tggtTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGc                                                                                                                                          <  1:1199614/150‑1 (MQ=255)
                ttCACGATTTGCAAAAAGCGATAGCTGAAATAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAATCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCtgt                                                                                                                                       <  2:121849/150‑1 (MQ=255)
                  cACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCtgttg                                                                                                                                     >  1:1600818/1‑150 (MQ=255)
                  cACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCtgttg                                                                                                                                     <  2:200589/150‑1 (MQ=255)
                    cGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTa                                                                                                                                   <  2:4008391/150‑1 (MQ=255)
                     gATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTAt                                                                                                                                  <  1:2802518/150‑1 (MQ=255)
                         tGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTgg                                                                                                                              >  2:2205884/1‑150 (MQ=255)
                         tGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTgg                                                                                                                              <  1:2454539/150‑1 (MQ=255)
                         tGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTgg                                                                                                                              >  2:3458594/1‑150 (MQ=255)
                               aaGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTcc                                                                                                                        <  1:1683159/150‑1 (MQ=255)
                                aGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCg                                                                                                                       <  2:124060/150‑1 (MQ=255)
                                 gCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGt                                                                                                                      >  1:684194/1‑150 (MQ=255)
                                  cGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTc                                                                                                                     <  1:207861/150‑1 (MQ=255)
                                  cGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTc                                                                                                                     >  1:4222342/1‑150 (MQ=255)
                                     tAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCggg                                                                                                                  <  1:4411542/150‑1 (MQ=255)
                                     tAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCggg                                                                                                                  <  2:1507991/150‑1 (MQ=255)
                                           aaaaaGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCg                                                                                                            >  1:4120009/1‑150 (MQ=255)
                                           aaaaaGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCg                                                                                                            >  1:565063/1‑150 (MQ=255)
                                              aaGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAACCGCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAg                                                                                                         <  2:259924/150‑1 (MQ=255)
                                              aaGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAg                                                                                                         >  2:1479954/1‑150 (MQ=255)
                                              aaGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAg                                                                                                         >  1:4523718/1‑150 (MQ=255)
                                              aaGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAg                                                                                                         >  2:527588/1‑150 (MQ=255)
                                               aGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGc                                                                                                        <  2:2234118/150‑1 (MQ=255)
                                                 tGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGt                                                                                                      >  2:4282392/1‑150 (MQ=255)
                                                   cAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTg                                                                                                    >  1:4092729/1‑150 (MQ=255)
                                                     gTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCt                                                                                                  >  1:3752903/1‑150 (MQ=255)
                                                      ttAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTg                                                                                                 >  1:943157/1‑150 (MQ=255)
                                                        aaaaCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGAt                                                                                               >  2:2804051/1‑150 (MQ=255)
                                                         aaaCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGAtt                                                                                              >  1:2883838/1‑150 (MQ=255)
                                                          aaCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTa                                                                                             <  1:610070/150‑1 (MQ=255)
                                                           aCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTAt                                                                                            >  1:2824544/1‑150 (MQ=255)
                                                           aCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTAt                                                                                            <  1:990413/150‑1 (MQ=255)
                                                            ccGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATc                                                                                           <  2:4209564/150‑1 (MQ=255)
                                                                 cACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATcagcag                                                                                      <  1:3064352/150‑1 (MQ=255)
                                                                 cACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATcagcag                                                                                      >  1:2953846/1‑150 (MQ=255)
                                                                 cACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATcagcag                                                                                      <  1:2565227/150‑1 (MQ=255)
                                                                  aCCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCagcagc                                                                                     >  1:1514552/1‑150 (MQ=255)
                                                                    cAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAACCTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGt                                                                                   <  2:4518567/150‑1 (MQ=255)
                                                                        gTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCg                                                                               >  2:3175994/1‑150 (MQ=255)
                                                                         tGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGt                                                                              >  1:1653480/1‑150 (MQ=255)
                                                                         tGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGt                                                                              >  1:1653372/1‑150 (MQ=255)
                                                                           gCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGttt                                                                            <  2:2529758/150‑1 (MQ=255)
                                                                             aTTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTgc                                                                          >  1:2543460/1‑150 (MQ=255)
                                                                             aTTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTgc                                                                          >  1:3659576/1‑150 (MQ=255)
                                                                               tctcCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTgcgc                                                                        >  1:1979651/1‑150 (MQ=255)
                                                                                 tcCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCtg                                                                      <  2:1321773/150‑1 (MQ=255)
                                                                                  ccGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCtgg                                                                     <  2:153509/150‑1 (MQ=255)
                                                                                   cGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCtggt                                                                    <  2:3836682/150‑1 (MQ=255)
                                                                                     ggCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCtggtgg                                                                  <  2:2830694/150‑1 (MQ=255)
                                                                                        aGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGTCGAAGCGTTGCTGATTATCAGCAGAGTACCGTTTGCGCGGGTGggcg                                                               >  1:35458/1‑150 (MQ=255)
                                                                                           tCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCa                                                            <  2:2928674/150‑1 (MQ=255)
                                                                                              aGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCt                                                         >  2:1793519/1‑150 (MQ=255)
                                                                                                cTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTgg                                                       >  2:110497/1‑150 (MQ=255)
                                                                                                cTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTgg                                                       >  2:466465/1‑150 (MQ=255)
                                                                                                 tACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGt                                                      <  1:540541/150‑1 (MQ=255)
                                                                                                  aCTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGtt                                                     >  1:4640827/1‑150 (MQ=255)
                                                                                                    tGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGGTTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGGTGc                                                   >  1:1138915/1‑150 (MQ=255)
                                                                                                     ggAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCt                                                  <  2:616551/150‑1 (MQ=255)
                                                                                                       aGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCtgt                                                >  1:3485411/1‑150 (MQ=255)
                                                                                                       aGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCtgt                                                >  2:4086875/1‑150 (MQ=255)
                                                                                                         cgcgcCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTgtgg                                              >  1:941037/1‑150 (MQ=255)
                                                                                                          gcgcCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTgtggt                                             >  2:2281665/1‑150 (MQ=255)
                                                                                                           cgcCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTgtggtg                                            <  1:2665765/150‑1 (MQ=255)
                                                                                                            gcCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGtggtgg                                           <  1:1799249/150‑1 (MQ=255)
                                                                                                            gcCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGtggtgg                                           >  2:1857355/1‑150 (MQ=255)
                                                                                                              cAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGAt                                         >  2:1038223/1‑150 (MQ=255)
                                                                                                              cAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGAt                                         <  1:3098864/150‑1 (MQ=255)
                                                                                                                 ccATAAGCTTAAACTCATGGTGCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATggg                                      >  2:1877171/1‑150 (MQ=255)
                                                                                                                 ccATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATggg                                      >  1:289485/1‑150 (MQ=255)
                                                                                                                    tAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCtt                                   >  1:1117121/1‑150 (MQ=255)
                                                                                                                       gCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCa                                >  2:165493/1‑150 (MQ=255)
                                                                                                                       gCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCa                                <  2:3581442/150‑1 (MQ=255)
                                                                                                                                cATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGt                       <  1:794187/150‑1 (MQ=255)
                                                                                                                                   ggTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGc                    <  2:3476085/150‑1 (MQ=255)
                                                                                                                                    gTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCg                   <  2:3674913/150‑1 (MQ=255)
                                                                                                                                      gCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGAc                 >  2:3382397/1‑150 (MQ=255)
                                                                                                                                      gCCGATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGAc                 >  2:3382428/1‑150 (MQ=255)
                                                                                                                                         gATGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACggg              >  1:811603/1‑150 (MQ=255)
                                                                                                                                           tGACGTTGATTATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCa            <  2:2460517/150‑1 (MQ=255)
                                                                                                                                           tGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCa            <  1:335958/150‑1 (MQ=255)
                                                                                                                                           tGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCa            >  1:2245351/1‑150 (MQ=255)
                                                                                                                                              cGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTg         >  1:3973303/1‑150 (MQ=255)
                                                                                                                                                ttGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGc       <  2:684194/150‑1 (MQ=255)
                                                                                                                                                ttGATGATTATCTTCGTGCGGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGc       >  2:3808723/1‑150 (MQ=255)
                                                                                                                                                  gatgatTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCATCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTAGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGCtt     >  2:782165/1‑150 (MQ=255)
                                                                                                                                                    tgatTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGCTTTa   >  2:2410956/1‑150 (MQ=255)
                                                                                                                                                    tgatTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCGGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGCTTTa   >  2:393890/1‑150 (MQ=255)
                                                                                                                                                     gatTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGCTTTAt  >  2:2831519/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATCTGGTTGCTGTGGTGGATGGGCTTTCATCTTTCCGTGGCGACGGGCACTGGCTTTAT  >  CP009273/596663‑596961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑