Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 613,755 G→T 32.0% D16Y (GAT→TAT)  fepE → regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273613,7550GT32.0% 437.5 / 191.4 227D16Y (GAT→TAT) fepEregulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base G (67/87);  new base T (40/33);  total (107/120)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

GGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGG  >  CP009273/613608‑613904
                                                                                                                                                   |                                                                                                                                                     
ggaaggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGACTATTAAACAGGGAAGTGACGCTCATTTTCCCTAt                                                                                                                                                     >  2:4220799/1‑150 (MQ=255)
ggaaggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTAt                                                                                                                                                     >  1:2755092/1‑150 (MQ=255)
 gaaggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTAtt                                                                                                                                                    >  1:2896302/1‑150 (MQ=255)
 gaaggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGAtt                                                                                                                                                    <  2:3702079/150‑1 (MQ=255)
 gaaggaaTTTACAAACTTCAGATTCTGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGAtt                                                                                                                                                    <  2:3029337/150‑1 (MQ=255)
  aaggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTa                                                                                                                                                   <  1:2591586/150‑1 (MQ=255)
   aggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCtattat                                                                                                                                                  <  2:2829707/150‑1 (MQ=255)
   aggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTAt                                                                                                                                                  >  1:1170834/1‑150 (MQ=255)
        tttACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCctct                                                                                                                                             <  2:1284193/150‑1 (MQ=255)
        tttACAAACTACAGCTTCGGATAATTATCACCACCTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCctct                                                                                                                                             <  2:1082491/150‑1 (MQ=255)
             aaaCTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGt                                                                                                                                        >  1:121892/1‑150 (MQ=255)
                 ttCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGcc                                                                                                                                    <  2:1788780/150‑1 (MQ=255)
                 ttCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGcc                                                                                                                                    <  2:3034383/150‑1 (MQ=255)
                  tCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGccc                                                                                                                                   >  1:4508178/1‑150 (MQ=255)
                   cAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCa                                                                                                                                  <  2:3099292/150‑1 (MQ=255)
                      cTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGta                                                                                                                               <  2:41302/150‑1 (MQ=255)
                        tCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGtaat                                                                                                                             >  1:1849860/1‑150 (MQ=255)
                        tCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTCCCCGATTATCCTCTGGCGTCGCCCAGtaat                                                                                                                             <  1:3374264/150‑1 (MQ=255)
                         cGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGtaata                                                                                                                            <  1:3681615/150‑1 (MQ=255)
                          ggATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGtaataa                                                                                                                           >  2:4319264/1‑150 (MQ=255)
                          ggATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACACCATGTCATCACTGAATATTAAACAGGGAAGTGAAGCTCATTTTACCGATTATCCTCTGGCGTCGCCCAGtaataa                                                                                                                           >  1:3483133/1‑150 (MQ=255)
                          ggATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATGTCCCCCTAGTGGACACATTTTCAGGTTATACCCCAAGACATCACTGAATAATAAACAGGGAAGTGACGCTCATTTTCCCGATTATACTCAGGCGTCGCCCAGtaataa                                                                                                                           >  1:27064/1‑150 (MQ=255)
                           gATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTaataat                                                                                                                          <  1:4182849/150‑1 (MQ=255)
                              aaTTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGac                                                                                                                       >  2:658778/1‑149 (MQ=255)
                              aaTTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGaa                                                                                                                       >  1:2929621/1‑150 (MQ=255)
                                ttATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAAt                                                                                                                     >  1:1732646/1‑150 (MQ=255)
                                  aTCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTg                                                                                                                   <  2:4372035/150‑1 (MQ=255)
                                   tCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGa                                                                                                                  <  1:4308022/150‑1 (MQ=255)
                                         tcTGGTATAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATAACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACt                                                                                                            <  2:924344/149‑1 (MQ=255)
                                         aCTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACt                                                                                                            <  2:730075/150‑1 (MQ=255)
                                         aCTGGTAAAGTGAGCGCCGTGGAGATGTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACt                                                                                                            <  2:2947944/150‑1 (MQ=255)
                                          cttttAAAGTGAGCGCCGTGGAGTTTTCCCACTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTACACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACtt                                                                                                           <  1:4494242/146‑1 (MQ=255)
                                          cTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACtt                                                                                                           <  1:2845590/150‑1 (MQ=255)
                                            ggTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaa                                                                                                         >  1:3869193/1‑150 (MQ=255)
                                            ggTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaa                                                                                                         <  2:782921/150‑1 (MQ=255)
                                            ggTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaa                                                                                                         <  2:1977483/150‑1 (MQ=255)
                                             gTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaat                                                                                                        <  2:4400196/150‑1 (MQ=255)
                                              tAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaatc                                                                                                       >  1:2022387/1‑150 (MQ=255)
                                              tAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaatc                                                                                                       <  2:2076967/150‑1 (MQ=255)
                                                    gAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTaatctaatct                                                                                                 >  2:2167697/1‑150 (MQ=255)
                                                     aGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTAATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAAtctc                                                                                                <  2:3577449/150‑1 (MQ=255)
                                                      gcgcCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCa                                                                                               <  2:2273996/150‑1 (MQ=255)
                                                      gcgcCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCa                                                                                               <  1:2260737/150‑1 (MQ=255)
                                                        gcCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGt                                                                                             <  2:1322908/150‑1 (MQ=255)
                                                          cGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGttt                                                                                           >  2:2344484/1‑150 (MQ=255)
                                                          cGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGttt                                                                                           <  1:2009495/150‑1 (MQ=255)
                                                            tGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTa                                                                                         <  2:3236220/150‑1 (MQ=255)
                                                            tGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTa                                                                                         >  1:326279/1‑150 (MQ=255)
                                                             ggAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTAt                                                                                        >  1:3065760/1‑150 (MQ=255)
                                                                 aTTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCg                                                                                    >  1:802726/1‑150 (MQ=255)
                                                                  tttCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCgg                                                                                   <  1:83827/150‑1 (MQ=255)
                                                                     cccccTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGcc                                                                                >  2:2514323/1‑150 (MQ=255)
                                                                     cccccTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGcc                                                                                <  2:3306014/150‑1 (MQ=255)
                                                                     cccccTATTGGACACATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGcc                                                                                <  2:481551/150‑1 (MQ=255)
                                                                       cccTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCaa                                                                              >  1:4377424/1‑150 (MQ=255)
                                                                         cTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCaaaa                                                                            <  1:4043734/150‑1 (MQ=255)
                                                                           aTTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCaaaaaa                                                                          <  2:801774/150‑1 (MQ=255)
                                                                             tGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAAc                                                                        <  1:1152382/150‑1 (MQ=255)
                                                                              ggACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTTTGGCGTGCCAAATAAACg                                                                       <  2:3893953/150‑1 (MQ=255)
                                                                                aCTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGt                                                                     <  2:2806550/150‑1 (MQ=255)
                                                                                aCTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGt                                                                     >  2:95209/1‑150 (MQ=255)
                                                                                    aTTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATg                                                                 <  1:103156/150‑1 (MQ=255)
                                                                                    aTTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTGAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATg                                                                 >  2:4601649/1‑150 (MQ=255)
                                                                                      tttCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGc                                                               <  1:2169154/150‑1 (MQ=255)
                                                                                       ttCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCg                                                              >  1:3017519/1‑150 (MQ=255)
                                                                                       ttCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCg                                                              <  1:3517097/150‑1 (MQ=255)
                                                                                            gTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGt                                                         >  2:886403/1‑150 (MQ=255)
                                                                                             ttATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGtt                                                        <  2:3333328/150‑1 (MQ=255)
                                                                                               ataCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGtttt                                                      <  2:936381/150‑1 (MQ=255)
                                                                                               ataCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTCTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGtttt                                                      <  2:936364/150‑1 (MQ=255)
                                                                                                taCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGttttt                                                     <  1:1653054/150‑1 (MQ=255)
                                                                                                  ccccATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGc                                                   <  2:2084439/150‑1 (MQ=255)
                                                                                                     cATGTCATCACTGAATATTAAACAGGGAAGNGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGtt                                                >  1:4283226/1‑150 (MQ=255)
                                                                                                       tGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTGTTTGGGTTTg                                              >  1:506152/1‑150 (MQ=255)
                                                                                                        gTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTTCGTTTGc                                             >  1:3675730/1‑150 (MQ=255)
                                                                                                          catcaCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCt                                           <  1:2418981/150‑1 (MQ=255)
                                                                                                          catcaCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCt                                           <  2:462397/150‑1 (MQ=255)
                                                                                                           atcaCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTg                                          >  1:1416990/1‑150 (MQ=255)
                                                                                                             caCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTTCGTTTTCCTgcg                                        >  2:1259216/1‑150 (MQ=255)
                                                                                                             caCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGGCGTTTTTGCGTTTGCCGgcg                                        >  2:1455108/1‑150 (MQ=255)
                                                                                                              aCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTTCGTTTGCCTgcgc                                       >  1:559184/1‑150 (MQ=255)
                                                                                                                tGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAg                                     <  1:3216803/150‑1 (MQ=255)
                                                                                                                 gAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAgg                                    >  2:155472/1‑150 (MQ=255)
                                                                                                                  aaTATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGc                                   <  2:874676/150‑1 (MQ=255)
                                                                                                                   atatTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGTCGTCGCCCAGTAATAATGAAATTGATTTACTTAATATAATCTCAGTTTTATGGCGGGCCAAAAAAACTGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCt                                  >  2:1179844/1‑150 (MQ=255)
                                                                                                                       tAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCt                              <  2:1849860/150‑1 (MQ=255)
                                                                                                                        aaaCAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTg                             <  2:2755092/150‑1 (MQ=255)
                                                                                                                         aaCAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGa                            <  1:3825884/150‑1 (MQ=255)
                                                                                                                         aaCAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGa                            >  1:3458221/1‑150 (MQ=255)
                                                                                                                            aGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGAtct                         >  2:3147601/1‑150 (MQ=255)
                                                                                                                              ggAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATctct                       <  1:683762/150‑1 (MQ=255)
                                                                                                                               gAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTTAGTTTGCCTGCGCAGGCTTGCTGAtatatt                      >  1:3441510/1‑145 (MQ=255)
                                                                                                                                      cGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCt               <  2:799998/150‑1 (MQ=255)
                                                                                                                                         tCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGcc            >  2:3440864/1‑150 (MQ=255)
                                                                                                                                         tCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGcc            >  2:4550989/1‑150 (MQ=255)
                                                                                                                                         tCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGcc            <  2:445333/150‑1 (MQ=255)
                                                                                                                                                cccTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCaaaaa     >  2:2050508/1‑150 (MQ=255)
                                                                                                                                                 ccGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAAt    >  2:1300215/1‑150 (MQ=255)
                                                                                                                                                 ccGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAAt    >  2:3744008/1‑150 (MQ=255)
                                                                                                                                                  cGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTGTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATg   >  2:1737966/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGG  >  CP009273/613608‑613904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑