Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 632,286 C→A 19.6% E263D (GAG→GAT)  ybdO ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273632,2860CA19.6% 829.4 / 132.9 284E263D (GAG→GAT) ybdOputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (117/111);  new base A (24/32);  total (141/143)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

AGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTC  >  CP009273/632137‑632435
                                                                                                                                                     |                                                                                                                                                     
aGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTAtc                                                                                                                                                       >  2:642656/1‑150 (MQ=255)
aGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTAtc                                                                                                                                                       <  2:2559622/150‑1 (MQ=255)
    gAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCtt                                                                                                                                                   >  1:2183836/1‑150 (MQ=255)
     aaaGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCttt                                                                                                                                                  <  1:2841473/150‑1 (MQ=255)
       aGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTtta                                                                                                                                                >  2:2532600/1‑150 (MQ=255)
        gTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTttaa                                                                                                                                               <  1:2264892/150‑1 (MQ=255)
         ttATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTttaag                                                                                                                                              >  2:268085/1‑150 (MQ=255)
          tataGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTttaagt                                                                                                                                             >  1:1745682/1‑150 (MQ=255)
            tagtagCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTttaagtta                                                                                                                                           >  1:1397523/1‑150 (MQ=255)
              gtagCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTttaagttaag                                                                                                                                         >  1:4554782/1‑150 (MQ=255)
                agCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTATGTTAAGaa                                                                                                                                       >  1:2302255/1‑150 (MQ=255)
                agCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGaa                                                                                                                                       >  1:4636934/1‑150 (MQ=255)
                  ctgtctgtAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAg                                                                                                                                     >  2:2848915/1‑150 (MQ=255)
                      ctgtAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCAc                                                                                                                                 >  1:4276503/1‑150 (MQ=255)
                      ctgtAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCAc                                                                                                                                 >  1:267895/1‑150 (MQ=255)
                       tgtAGTTATATATTAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACg                                                                                                                                >  2:1786430/1‑150 (MQ=255)
                         tAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGt                                                                                                                              <  1:4090131/150‑1 (MQ=255)
                           gTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAg                                                                                                                            <  1:4100759/150‑1 (MQ=255)
                           gTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAg                                                                                                                            >  2:1888957/1‑150 (MQ=255)
                             tatataTCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGa                                                                                                                          <  1:2952400/150‑1 (MQ=255)
                                   tCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATtata                                                                                                                    <  2:2311150/150‑1 (MQ=255)
                                     aaaaaCTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATaaa                                                                                                                  >  2:4138968/1‑150 (MQ=255)
                                     aaaaaCTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATaaa                                                                                                                  <  2:3542647/150‑1 (MQ=255)
                                     aaaaaCTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATaaa                                                                                                                  <  1:1950676/150‑1 (MQ=255)
                                        aaCTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGtt                                                                                                               >  1:3124692/1‑150 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        >  1:1501080/1‑150 (MQ=255)
                                               aTTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAggg                                                                                                        >  1:1500612/1‑150 (MQ=255)
                                                   aGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACt                                                                                                    >  1:678696/1‑150 (MQ=255)
                                                   aGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACt                                                                                                    <  2:479962/150‑1 (MQ=255)
                                                   aGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACt                                                                                                    >  1:1191958/1‑150 (MQ=255)
                                                     aCGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTAt                                                                                                  <  1:1845951/150‑1 (MQ=255)
                                                       ggTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTc                                                                                                >  1:122311/1‑150 (MQ=255)
                                                       ggTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTc                                                                                                <  2:3250206/150‑1 (MQ=255)
                                                       ggTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTc                                                                                                <  1:3880974/150‑1 (MQ=255)
                                                       ggTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATATTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTc                                                                                                >  1:4400985/1‑150 (MQ=255)
                                                        gTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTCACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTcc                                                                                               >  1:3399878/1‑150 (MQ=255)
                                                           aaTAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAg                                                                                            >  2:2264505/1‑150 (MQ=255)
                                                            aTAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGt                                                                                           >  2:3397473/1‑150 (MQ=255)
                                                            aTAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGt                                                                                           >  2:4241293/1‑150 (MQ=255)
                                                                aTCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATGATAAAGTTCATAGGGGACTATTCCCAGTAAAt                                                                                       >  2:2041693/1‑150 (MQ=255)
                                                                 tCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATc                                                                                      <  2:518449/150‑1 (MQ=255)
                                                                  cGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCt                                                                                     <  1:2770337/150‑1 (MQ=255)
                                                                   gggAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTg                                                                                    >  1:511915/1‑150 (MQ=255)
                                                                        aaCACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACt                                                                               >  1:1077341/1‑150 (MQ=255)
                                                                            acAAATTATTTAAAGAAGATTTATTATAGTAAATGTAAAGCTTTATTGAAGGTAACTGATGTTCTAGAATTATCTCATTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGTACTATTCCCAGTAAATCTTTTACAGCaa                                                                           >  2:733191/2‑150 (MQ=255)
                                                                                   attTAAAGAAGATTTATTATAGGACATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTAtt                                                                    >  1:3709671/1‑150 (MQ=255)
                                                                                     ttAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTaa                                                                  <  2:2450317/150‑1 (MQ=255)
                                                                                      tAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAAt                                                                 <  2:1232191/150‑1 (MQ=255)
                                                                                       aaaGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATg                                                                >  1:864047/1‑150 (MQ=255)
                                                                                       aaaGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATg                                                                <  2:1909993/150‑1 (MQ=255)
                                                                                        aagaagATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGa                                                               >  1:77715/1‑150 (MQ=255)
                                                                                        aagaagATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGa                                                               <  1:4241293/150‑1 (MQ=255)
                                                                                         agaagaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGAt                                                              >  1:636383/1‑150 (MQ=255)
                                                                                          gaagaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTATGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGAtt                                                             >  2:616422/1‑150 (MQ=255)
                                                                                           aagaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTg                                                            >  1:3905173/1‑150 (MQ=255)
                                                                                           aagaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTg                                                            >  2:4526637/1‑150 (MQ=255)
                                                                                            agaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGt                                                           >  2:2929666/1‑150 (MQ=255)
                                                                                             gaTTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTc                                                          >  2:1678743/1‑150 (MQ=255)
                                                                                                 tattatAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCa                                                      >  1:1061482/1‑150 (MQ=255)
                                                                                                   ttatAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCaaa                                                    >  1:4336561/1‑150 (MQ=255)
                                                                                                    tataGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAag                                                   <  2:1023414/150‑1 (MQ=255)
                                                                                                      taGGAAATGTAAATATTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAagag                                                 <  2:3950152/150‑1 (MQ=255)
                                                                                                      taGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAagag                                                 <  2:3230705/150‑1 (MQ=255)
                                                                                                        ggAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGa                                               >  1:503701/1‑150 (MQ=255)
                                                                                                          aaaTGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAAc                                             <  1:4317190/150‑1 (MQ=255)
                                                                                                              gTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACg                                         <  1:3044086/150‑1 (MQ=255)
                                                                                                               tAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGa                                        >  1:2409931/1‑150 (MQ=255)
                                                                                                                aaaGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGaa                                       >  2:1185840/1‑150 (MQ=255)
                                                                                                                aaaGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGaa                                       >  2:4169556/1‑150 (MQ=255)
                                                                                                                 aaGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGaaa                                      <  2:4337166/150‑1 (MQ=255)
                                                                                                                 aaGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGaaa                                      <  2:3796031/150‑1 (MQ=255)
                                                                                                                  aGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAAt                                     <  2:1377138/150‑1 (MQ=255)
                                                                                                                   gCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATg                                    >  1:3630635/1‑150 (MQ=255)
                                                                                                                   gCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATg                                    <  2:2232887/150‑1 (MQ=255)
                                                                                                                   gCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATg                                    >  1:2121817/1‑150 (MQ=255)
                                                                                                                   gCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATg                                    >  2:1793727/1‑150 (MQ=255)
                                                                                                                   gCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATg                                    <  2:2860766/150‑1 (MQ=255)
                                                                                                                     tttATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATgag                                  <  2:945459/150‑1 (MQ=255)
                                                                                                                     tttATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATgag                                  <  2:306596/150‑1 (MQ=255)
                                                                                                                       tATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAt                                >  2:2964468/1‑150 (MQ=255)
                                                                                                                       tATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAt                                <  1:1787754/150‑1 (MQ=255)
                                                                                                                       tATTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAt                                >  2:2848090/1‑150 (MQ=255)
                                                                                                                        aTTGAAGGTAACGGATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAtt                               >  1:3221674/1‑150 (MQ=255)
                                                                                                                          tGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAtttt                             <  1:1355431/150‑1 (MQ=255)
                                                                                                                                tAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGAtt                       <  1:3640442/150‑1 (MQ=255)
                                                                                                                                 aaCGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTc                      >  1:1100327/1‑150 (MQ=255)
                                                                                                                                    ggATGTTCTAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATa                   >  2:3200985/1‑150 (MQ=255)
                                                                                                                                        gTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtc               >  1:2391777/1‑150 (MQ=255)
                                                                                                                                         ttCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtct              <  1:788398/150‑1 (MQ=255)
                                                                                                                                          tCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtctc             >  2:1256438/1‑150 (MQ=255)
                                                                                                                                            tAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtctctc           <  2:4352255/150‑1 (MQ=255)
                                                                                                                                            tAGTTTTATATCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtctctc           >  1:3389437/1‑150 (MQ=255)
                                                                                                                                              gTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGt         <  1:2741300/150‑1 (MQ=255)
                                                                                                                                                tttATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCt       <  2:2068352/150‑1 (MQ=255)
                                                                                                                                                  tATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTAt     <  2:4430347/150‑1 (MQ=255)
                                                                                                                                                  tATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTAt     >  1:2466305/1‑150 (MQ=255)
                                                                                                                                                   aTCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTAtt    <  2:1340041/150‑1 (MQ=255)
                                                                                                                                                   aTCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTAtt    <  1:989744/150‑1 (MQ=255)
                                                                                                                                                    tctcTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTAttt   <  1:2063077/150‑1 (MQ=255)
                                                                                                                                                     ctctTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTc  >  1:237235/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATCAAAAACTCTCATTTAGACGGTCAATAAATCGGGAGAACACCAAATTATTTAAAGAAGATTTATTATAGGAAATGTAAAGCTTTATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTC  >  CP009273/632137‑632435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑