Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 706,479 C→A 57.4% R148L (CGT→CTT)  fldA ← flavodoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273706,4790CA57.4% 15.7 / inf 237R148L (CGT→CTT) fldAflavodoxin 1
Reads supporting (aligned to +/- strand):  ref base C (62/39);  new base A (82/54);  total (144/93)
Fisher's exact test for biased strand distribution p-value = 8.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 239 total aligned reads displayed.

AATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCGCA  >  CP009273/706330‑706627
                                                                                                                                                     |                                                                                                                                                    
aaTTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAc                                                                                                                                                      <  1:745014/150‑1 (MQ=255)
   tattTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGt                                                                                                                                                   >  2:4317877/1‑150 (MQ=255)
    attTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTc                                                                                                                                                  <  1:3404599/150‑1 (MQ=255)
           ttGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTtcgtcg                                                                                                                                           <  2:106487/150‑1 (MQ=255)
            tGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGa                                                                                                                                          >  2:500891/1‑150 (MQ=255)
            tGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGa                                                                                                                                          <  1:2200402/150‑1 (MQ=255)
             ggCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGAt                                                                                                                                         >  1:3253695/1‑150 (MQ=255)
               cATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACCTCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAg                                                                                                                                       <  1:3994129/150‑1 (MQ=255)
               cATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGACTTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAg                                                                                                                                       >  2:1721699/1‑150 (MQ=255)
               cATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAg                                                                                                                                       >  2:1721905/1‑150 (MQ=255)
               cATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAg                                                                                                                                       >  1:959179/1‑150 (MQ=255)
                   aTCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAg                                                                                                                                   >  1:500557/1‑150 (MQ=255)
                     cTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGAc                                                                                                                                 >  2:1885361/1‑150 (MQ=255)
                      tATTAATACGGCGTAGACACGAGTCTACGCCGCATCCCCTCAGGCATTGAGACTGTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGAcc                                                                                                                                <  2:3317433/150‑1 (MQ=255)
                       aTTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCg                                                                                                                               >  2:2042471/1‑150 (MQ=255)
                         tAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGAc                                                                                                                             <  2:180707/150‑1 (MQ=255)
                             aCGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAg                                                                                                                         >  1:1566594/1‑150 (MQ=255)
                              cGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGt                                                                                                                        >  1:894522/1‑150 (MQ=255)
                                gCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTgg                                                                                                                      <  1:1043251/150‑1 (MQ=255)
                                 cGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATGTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGgt                                                                                                                     <  2:2890831/150‑1 (MQ=255)
                                    aGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGgtcgt                                                                                                                  >  1:4617853/1‑150 (MQ=255)
                                      aCATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCa                                                                                                                >  2:238994/1‑150 (MQ=255)
                                         tGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCt                                                                                                             <  1:1014231/150‑1 (MQ=255)
                                            gTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTATTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGcc                                                                                                          >  2:667030/1‑150 (MQ=255)
                                                aCGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGAc                                                                                                      >  1:314854/1‑150 (MQ=255)
                                                    cgcATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCttt                                                                                                  <  1:1694298/150‑1 (MQ=255)
                                                    cgcATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGATCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCttt                                                                                                  >  1:4069459/1‑150 (MQ=255)
                                                     gcATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCtttt                                                                                                 <  1:2324407/150‑1 (MQ=255)
                                                      catcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTg                                                                                                >  1:3008154/1‑150 (MQ=255)
                                                      catcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTg                                                                                                <  1:4297390/150‑1 (MQ=255)
                                                       atcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGa                                                                                               <  2:2057553/150‑1 (MQ=255)
                                                       atcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGa                                                                                               >  2:1581537/1‑150 (MQ=255)
                                                        tcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGAt                                                                                              <  2:2852314/150‑1 (MQ=255)
                                                        tcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGAt                                                                                              >  1:867257/1‑150 (MQ=255)
                                                        tcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGAt                                                                                              <  1:2474921/150‑1 (MQ=255)
                                                          acctcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGc                                                                                            <  2:3740772/147‑1 (MQ=255)
                                                             tcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTc                                                                                         <  1:132752/150‑1 (MQ=255)
                                                                ggCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGaa                                                                                      >  2:3150362/1‑150 (MQ=255)
                                                                  cATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAAt                                                                                    >  1:1812088/1‑150 (MQ=255)
                                                                     tGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGAt                                                                                 <  2:4484360/150‑1 (MQ=255)
                                                                       agaATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAg                                                                               >  1:2367806/1‑150 (MQ=255)
                                                                         aaTTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGcc                                                                             <  1:3115599/150‑1 (MQ=255)
                                                                         aaTTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGcc                                                                             >  2:2991743/1‑150 (MQ=255)
                                                                          aTTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGccc                                                                            <  1:3825564/150‑1 (MQ=255)
                                                                                 cgAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTg                                                                     >  2:2397023/1‑150 (MQ=255)
                                                                                   agaTGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGc                                                                   >  1:683242/1‑150 (MQ=255)
                                                                                    gaTGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGcc                                                                  >  1:3057343/1‑150 (MQ=255)
                                                                                       gCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGt                                                               <  2:3353864/150‑1 (MQ=255)
                                                                                        cAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTg                                                              >  1:562127/1‑150 (MQ=255)
                                                                                         aaCTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGa                                                             >  2:283683/1‑150 (MQ=255)
                                                                                         aaCTCGTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGa                                                             <  2:4074490/150‑1 (MQ=255)
                                                                                           ctctTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGAcc                                                           >  2:3529263/1‑150 (MQ=255)
                                                                                              ttCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAAc                                                        >  1:4310526/1‑150 (MQ=255)
                                                                                               tCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACg                                                       >  2:1351725/1‑150 (MQ=255)
                                                                                               tCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACg                                                       >  2:3001228/1‑150 (MQ=255)
                                                                                               tCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACg                                                       <  2:769076/150‑1 (MQ=255)
                                                                                                 aGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGAt                                                     >  1:3858935/1‑150 (MQ=255)
                                                                                                 aGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGAt                                                     <  2:2651297/150‑1 (MQ=255)
                                                                                                  gAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATg                                                    <  1:3535506/150‑1 (MQ=255)
                                                                                                  gAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATg                                                    >  2:1982259/1‑150 (MQ=255)
                                                                                                   aaaTCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATgg                                                   >  1:1864916/1‑150 (MQ=255)
                                                                                                    aaTCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGt                                                  >  1:2614871/1‑150 (MQ=255)
                                                                                                     aTCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGtt                                                 >  2:2115007/1‑150 (MQ=255)
                                                                                                        tGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCa                                              >  2:797216/1‑150 (MQ=255)
                                                                                                         gTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCAc                                             >  1:4509839/1‑150 (MQ=255)
                                                                                                          tttAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCAcc                                            >  1:381499/1‑150 (MQ=255)
                                                                                                            tAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACcgc                                          >  1:1916532/1‑150 (MQ=255)
                                                                                                              aCCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACcgcgc                                        <  2:4604606/150‑1 (MQ=255)
                                                                                                              aCCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACcgcgc                                        >  1:2409844/1‑150 (MQ=255)
                                                                                                               cccATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACcgcgcg                                       <  2:1153538/150‑1 (MQ=255)
                                                                                                               cccATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACcgcgcg                                       >  1:1331604/1‑150 (MQ=255)
                                                                                                                ccATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCgg                                      >  2:163932/1‑150 (MQ=255)
                                                                                                                ccATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCgg                                      >  2:4207477/1‑150 (MQ=255)
                                                                                                                 cATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGt                                     <  2:1565401/150‑1 (MQ=255)
                                                                                                                  aTTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGtt                                    >  1:3811248/1‑150 (MQ=255)
                                                                                                                   tttttCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTc                                   <  2:2182164/150‑1 (MQ=255)
                                                                                                                    ttttCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCa                                  <  1:524490/150‑1 (MQ=255)
                                                                                                                       tCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatg                               <  2:876668/150‑1 (MQ=255)
                                                                                                                        cTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatga                              >  1:1077996/1‑150 (MQ=255)
                                                                                                                          acacGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgatg                            >  2:1766706/1‑150 (MQ=255)
                                                                                                                          acacGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgatg                            <  2:3829479/150‑1 (MQ=255)
                                                                                                                            acGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTc                          <  2:3200499/150‑1 (MQ=255)
                                                                                                                            acGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTc                          <  2:4264500/150‑1 (MQ=255)
                                                                                                                             cGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTcg                         >  2:3099767/1‑150 (MQ=255)
                                                                                                                                  aGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGAt                    >  2:248763/1‑150 (MQ=255)
                                                                                                                                     ggTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGt                 <  2:1386752/150‑1 (MQ=255)
                                                                                                                                     ggTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGt                 >  1:4263954/1‑150 (MQ=255)
                                                                                                                                     ggTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGt                 >  2:2317494/1‑150 (MQ=255)
                                                                                                                                         aGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGTCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGccc             >  2:3290540/1‑150 (MQ=255)
                                                                                                                                         aGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGccc             >  1:4107442/1‑150 (MQ=255)
                                                                                                                                          gTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCa            <  2:3253695/150‑1 (MQ=255)
                                                                                                                                           ttCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCaa           <  1:2991843/150‑1 (MQ=255)
                                                                                                                                            tCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAAt          >  2:1483939/1‑150 (MQ=255)
                                                                                                                                               ggCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCg       <  2:1956330/150‑1 (MQ=255)
                                                                                                                                               ggCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCg       >  1:4537042/1‑150 (MQ=255)
                                                                                                                                               ggCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCg       <  2:2228642/150‑1 (MQ=255)
                                                                                                                                               ggCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCg       >  2:4644130/1‑150 (MQ=255)
                                                                                                                                                  tGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCg    >  1:1465851/1‑150 (MQ=255)
                                                                                                                                                   gACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCGc   >  2:3593078/1‑150 (MQ=255)
                                                                                                                                                    aCGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCGCa  >  1:2585044/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCGCA  >  CP009273/706330‑706627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑