Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 776,049 C→A 16.8% noncoding (40/76 nt) lysT → tRNA‑Lys

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273776,0490CA16.8% 636.7 / 78.5 212noncoding (40/76 nt)lysTtRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base C (84/92);  new base A (12/24);  total (96/116)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 215 total aligned reads displayed.

AAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAG  >  CP009273/775900‑776198
                                                                                                                                                     |                                                                                                                                                     
aaGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                                                                       <  2:783269/150‑1 (MQ=255)
aaGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                                                                       >  2:1301215/1‑150 (MQ=255)
   cAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                                                                    >  2:2990831/1‑150 (MQ=255)
      ttGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATCTGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                                                                                                                                 <  1:2860911/150‑1 (MQ=255)
      ttGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAATCAATTgg                                                                                                                                                 >  1:1291286/1‑150 (MQ=255)
       tGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                                                                                                                                <  1:2314755/150‑1 (MQ=255)
        gAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTc                                                                                                                                               <  1:2697536/150‑1 (MQ=255)
          gTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGc                                                                                                                                             >  1:1645554/1‑150 (MQ=255)
            gATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAg                                                                                                                                           >  1:113820/1‑150 (MQ=255)
                tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                                                                       >  1:1179215/1‑150 (MQ=255)
                tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                                                                       <  1:3322113/150‑1 (MQ=255)
                tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                                                                       <  1:1654069/150‑1 (MQ=255)
                tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                                                                       <  2:3515627/150‑1 (MQ=255)
                tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAATGGTAGCAGGTTc                                                                                                                                       >  1:3858602/1‑150 (MQ=255)
                 aCATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCg                                                                                                                                      <  1:3349762/150‑1 (MQ=255)
                    tCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                                                                   <  1:1955174/150‑1 (MQ=255)
                     cGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAATGGTCGCAGGTTCGAATc                                                                                                                                  >  2:239520/1‑150 (MQ=255)
                      gAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATcc                                                                                                                                 <  1:2276013/150‑1 (MQ=255)
                      gAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATcc                                                                                                                                 <  1:77664/150‑1 (MQ=255)
                       aaaTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                                                                                >  1:4298228/1‑150 (MQ=255)
                       aaaTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                                                                                <  1:3633977/150‑1 (MQ=255)
                       aaaTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                                                                                >  2:3749471/1‑150 (MQ=255)
                       aaaTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                                                                                <  2:4203124/150‑1 (MQ=255)
                        aaTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTg                                                                                                                               >  2:1458874/1‑150 (MQ=255)
                        aaTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTg                                                                                                                               <  2:1266909/150‑1 (MQ=255)
                           ttttGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAc                                                                                                                            >  1:534190/1‑150 (MQ=255)
                                ttgCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc                                                                                                                       >  2:181958/1‑150 (MQ=255)
                                    gcTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccacca                                                                                                                   >  2:1147985/1‑150 (MQ=255)
                                     cTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCaa                                                                                                                  >  2:653960/1‑150 (MQ=255)
                                      tCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAt                                                                                                                 <  1:292910/150‑1 (MQ=255)
                                       cAAGTCTGACATCAGTAATATATGCCGCCGTTGCAACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGATGGTAGAGCAGTTGACAATTAATCAAATGGTCGCAGGTTCGAATCCAGCACGACCCACCAATc                                                                                                                >  2:2394732/1‑150 (MQ=255)
                                       cAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATc                                                                                                                <  2:817952/150‑1 (MQ=255)
                                       cAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATc                                                                                                                >  2:2394241/1‑150 (MQ=255)
                                       cAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATc                                                                                                                <  2:1503590/150‑1 (MQ=255)
                                         aGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAACCACCAATCGc                                                                                                              >  2:2985104/1‑150 (MQ=255)
                                          gTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCt                                                                                                             <  1:968961/150‑1 (MQ=255)
                                          gTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCt                                                                                                             <  1:969017/150‑1 (MQ=255)
                                             tGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAg                                                                                                          >  2:3231410/1‑150 (MQ=255)
                                               aaaTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGt                                                                                                        >  2:3258622/1‑150 (MQ=255)
                                                aaTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTg                                                                                                       <  1:3468603/150‑1 (MQ=255)
                                                 aTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAATAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTgg                                                                                                      <  1:2073560/150‑1 (MQ=255)
                                                  tCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGa                                                                                                     <  2:3346975/150‑1 (MQ=255)
                                                   cAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGaa                                                                                                    >  2:181434/1‑150 (MQ=255)
                                                     gTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGc                                                                                                  <  2:3712856/150‑1 (MQ=255)
                                                      tAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCg                                                                                                 <  2:469917/150‑1 (MQ=255)
                                                      tAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCg                                                                                                 <  2:470013/150‑1 (MQ=255)
                                                         tatatGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGgta                                                                                              >  1:3690921/1‑150 (MQ=255)
                                                              gccgccGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTaaa                                                                                         >  1:714774/1‑150 (MQ=255)
                                                              gccgccGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTaaa                                                                                         >  1:268163/1‑150 (MQ=255)
                                                                  ccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGt                                                                                     >  2:3796191/1‑150 (MQ=255)
                                                                   cgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTg                                                                                    >  1:4620349/1‑150 (MQ=255)
                                                                     ttGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGaa                                                                                  <  1:3047826/150‑1 (MQ=255)
                                                                     ttGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGaa                                                                                  <  2:1229674/150‑1 (MQ=255)
                                                                     ttGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGaa                                                                                  <  1:1499037/150‑1 (MQ=255)
                                                                      tGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAg                                                                                 >  2:219925/1‑150 (MQ=255)
                                                                        ccACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGa                                                                               <  1:1521770/150‑1 (MQ=255)
                                                                         cACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGAt                                                                              <  2:2454701/150‑1 (MQ=255)
                                                                         cACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGAt                                                                              <  1:181020/150‑1 (MQ=255)
                                                                           cGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATaa                                                                            <  1:1980987/150‑1 (MQ=255)
                                                                                   caaacaaaCCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCAt                                                                    >  2:2527096/1‑150 (MQ=255)
                                                                                    aaacaaacCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATg                                                                   <  1:3614986/150‑1 (MQ=255)
                                                                                      acaaacCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAg                                                                 <  2:90553/150‑1 (MQ=255)
                                                                                      acaaacCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATACTGCACGACCCACCAATCGCTAAGGTTGAAGCGGTAGTAAAACGTGAAGGATAACGTAGCATGAg                                                                 >  1:3531522/1‑150 (MQ=255)
                                                                                         aacCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCaa                                                              >  1:2979491/1‑150 (MQ=255)
                                                                                          acCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAAc                                                             <  1:175473/150‑1 (MQ=255)
                                                                                            cGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACgg                                                           <  2:3202499/150‑1 (MQ=255)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCg                                                       <  2:27234/150‑1 (MQ=255)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGGAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCg                                                       >  1:4183702/1‑150 (MQ=255)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATATATAAGGAGGAAGCGGTAGTAAAACGAGAATGATAAAGGTGCATGAGCAACTGCCCg                                                       >  2:447240/1‑150 (MQ=18)
                                                                                                aGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCg                                                       >  1:3098102/1‑150 (MQ=255)
                                                                                                 gCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCa                                                                                                                             >  2:1079394/1‑79 (MQ=255)
                                                                                                 gCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGa                                                      <  2:4056405/150‑1 (MQ=255)
                                                                                                 gCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGa                                                      <  2:4056700/150‑1 (MQ=255)
                                                                                                  cAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGaa                                                     >  1:2935584/1‑150 (MQ=255)
                                                                                                  cAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATAGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGGGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGaa                                                     >  2:3440960/1‑150 (MQ=255)
                                                                                                   aaCGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAg                                                    <  2:4537542/150‑1 (MQ=255)
                                                                                                      gAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGc                                                 >  1:3261653/1‑150 (MQ=255)
                                                                                                       aaaaaGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCg                                                <  1:3919149/150‑1 (MQ=255)
                                                                                                        aaaaGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCga                                               <  1:376670/150‑1 (MQ=255)
                                                                                                             tGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGa                                          <  1:4362921/150‑1 (MQ=255)
                                                                                                               ggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAg                                        <  2:1878526/150‑1 (MQ=255)
                                                                                                                   gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGa                                    <  1:2249863/150‑1 (MQ=255)
                                                                                                                     tAGCTCAGTTGGTAGAGCAGTTGACTTTTACTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGCCGAGt                                  <  1:1023330/150‑1 (MQ=255)
                                                                                                                     tAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGt                                  >  2:1008978/1‑150 (MQ=255)
                                                                                                                      aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTc                                 <  1:4544222/150‑1 (MQ=255)
                                                                                                                        cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCAt                               <  1:3351906/150‑1 (MQ=255)
                                                                                                                        cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCAt                               >  2:2964114/1‑150 (MQ=255)
                                                                                                                         tCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATc                              >  2:2209691/1‑150 (MQ=255)
                                                                                                                           aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCt                            <  1:1424569/150‑1 (MQ=255)
                                                                                                                               ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                        >  2:2802275/1‑150 (MQ=255)
                                                                                                                                  agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAc                     >  2:4274556/1‑150 (MQ=255)
                                                                                                                                  agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAc                     <  2:1344126/150‑1 (MQ=255)
                                                                                                                                      cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccac                 <  2:1645554/150‑1 (MQ=255)
                                                                                                                                       aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCTCTAAGGGGGACGCGGTAGTAAAACGGGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCAACCTGCACGACccacc                >  2:2305006/1‑150 (MQ=18)
                                                                                                                                       aGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccacc                <  1:191335/150‑1 (MQ=255)
                                                                                                                                        gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccacca               >  1:2514496/1‑150 (MQ=255)
                                                                                                                                        gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccacca               <  2:1447001/150‑1 (MQ=255)
                                                                                                                                              ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATAGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCAACCAGCACGACCCACCACTAACa         >  1:605998/1‑150 (MQ=255)
                                                                                                                                               tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACAt        >  2:3557167/1‑150 (MQ=255)
                                                                                                                                                ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACAta       >  1:3837118/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAG  >  CP009273/775900‑776198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑