BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	832,317	C→A	6.3%	R171S (CGT→AGT)	ybiC →	putative dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	832,317	0	C	A	6.3%	980.8 / 26.4	255	R171S (CGT→AGT)	ybiC	putative dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (139/100); new base A (8/8); total (147/108)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 263 total aligned reads displayed.

TCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATG  >  CP009273/832168‑832466

tCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTc                                                                                                                                                       >  1:3209303/1‑150 (MQ=255)

tCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTc                                                                                                                                                       <  1:2995319/150‑1 (MQ=255)

tCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTc                                                                                                                                                       <  2:2896738/150‑1 (MQ=255)

tCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTc                                                                                                                                                       <  2:4302414/150‑1 (MQ=255)

tCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTc                                                                                                                                                       <  1:2126084/150‑1 (MQ=255)

 cGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCg                                                                                                                                                      <  1:4446558/150‑1 (MQ=255)

  ggCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGt                                                                                                                                                     <  2:131825/150‑1 (MQ=255)

  ggCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTAGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTAGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGt                                                                                                                                                     >  1:3459463/1‑150 (MQ=255)

    ccGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTaa                                                                                                                                                   <  2:2286824/150‑1 (MQ=255)

      gTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAg                                                                                                                                                 >  1:4192961/1‑150 (MQ=255)

          cGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATaa                                                                                                                                             >  1:3916440/1‑150 (MQ=255)

              tACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTc                                                                                                                                         >  1:419206/1‑150 (MQ=255)

               aCTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTcc                                                                                                                                        >  2:3384543/1‑150 (MQ=255)

                 tGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGG‑TTTCCCTCGTAAAGATAATTTCCCGc                                                                                                                                     >  1:1321319/1‑150 (MQ=255)

                 tGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGG‑TTTCCCTCGTAAAGATAATTTCCCGc                                                                                                                                     >  1:3069526/1‑150 (MQ=255)

                     cGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGt                                                                                                                                  <  2:3949044/150‑1 (MQ=255)

                       gAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACGTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTg                                                                                                                                >  2:2965877/1‑150 (MQ=255)

                          cAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCtt                                                                                                                             <  1:621982/150‑1 (MQ=255)

                           aGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTg                                                                                                                            <  2:4263180/150‑1 (MQ=255)

                           aGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTg                                                                                                                            >  2:2743101/1‑150 (MQ=255)

                            gtgtGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGa                                                                                                                           <  2:2969705/150‑1 (MQ=255)

                                  aGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGc                                                                                                                     >  1:1290242/1‑150 (MQ=255)

                                  aGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTAGTAAAGATAATTTCCCGCTGTTGCTTGATTACGc                                                                                                                     >  1:1003046/1‑150 (MQ=255)

                                  aGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTAGTAAAGATAATTTCCCGCTGTTGCTTGATTACGc                                                                                                                     >  1:1002993/1‑150 (MQ=255)

                                     ggcggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGccac                                                                                                                  >  2:2754298/1‑150 (MQ=255)

                                      gcggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGccacc                                                                                                                 <  2:4045242/150‑1 (MQ=255)

                                       cggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGccacca                                                                                                                >  2:1898316/1‑150 (MQ=255)

                                       cggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGccacca                                                                                                                <  2:1803231/150‑1 (MQ=255)

                                          ggTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAgcg                                                                                                             <  1:2139439/150‑1 (MQ=255)

                                          ggTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAgcg                                                                                                             <  2:766868/150‑1 (MQ=255)

                                            tttGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGAGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCGTGATTACGCCACCAGCGcc                                                                                                           >  2:4228875/1‑150 (MQ=255)

                                              tGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCAt                                                                                                         <  1:70694/150‑1 (MQ=255)

                                              tGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCAt                                                                                                         >  1:2017378/1‑150 (MQ=255)

                                              tGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCAt                                                                                                         >  1:1851690/1‑150 (MQ=255)

                                                tctcTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTg                                                                                                       >  1:4507460/1‑150 (MQ=255)

                                                    tATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCAtt                                                                                                   >  2:4342617/1‑150 (MQ=255)

                                                    tATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTATTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCAtt                                                                                                   >  1:2348628/1‑150 (MQ=255)

                                                        cACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGc                                                                                               <  2:2872651/150‑1 (MQ=255)

                                                             tggtAGCGTGGTCGGTATTCCTATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCTCCAATCCGTTCTGTGTGGTTTTCCCTCGTATAGATATTTTCACGCTTTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAAc                                                                                          <  2:2495401/147‑1 (MQ=255)

                                                              gTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAAcc                                                                                         <  1:794853/150‑1 (MQ=255)

                                                                tAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCcg                                                                                       <  2:2627412/150‑1 (MQ=255)

                                                                tAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCcg                                                                                       <  1:2044206/150‑1 (MQ=255)

                                                                tAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCcg                                                                                       <  2:61845/150‑1 (MQ=255)

                                                                tAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCcg                                                                                       <  2:309299/150‑1 (MQ=255)

                                                                   cGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGt                                                                                    >  2:2873140/1‑150 (MQ=255)

                                                                    gTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTc                                                                                   >  2:1080444/1‑150 (MQ=255)

                                                                       gTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGcc                                                                                <  1:1067111/150‑1 (MQ=255)

                                                                       gTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTAGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGcc                                                                                <  1:1648383/150‑1 (MQ=255)

                                                                       gTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTAGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGcc                                                                                <  1:1648411/150‑1 (MQ=255)

                                                                         cGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCTCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTg                                                                              <  1:2785376/150‑1 (MQ=255)

                                                                          ggTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTgg                                                                             <  2:834850/150‑1 (MQ=255)

                                                                           gTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGc                                                                            >  2:2024966/1‑150 (MQ=255)

                                                                            tATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCAGTTGGCAAAACCCGCGTCGCCTGGCa                                                                           >  2:367100/1‑150 (MQ=255)

                                                                               tCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATaa                                                                        <  1:2598704/150‑1 (MQ=255)

                                                                                 cAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAg                                                                      >  2:3197692/1‑150 (MQ=255)

                                                                                 cAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAg                                                                      >  2:2551445/1‑150 (MQ=255)

                                                                                   aTGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGc                                                                    <  2:807188/150‑1 (MQ=255)

                                                                                   aTGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGc                                                                    <  1:337029/150‑1 (MQ=255)

                                                                                     ggTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGt                                                                  >  2:1280300/1‑150 (MQ=255)

                                                                                       tCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTcc                                                                <  2:1603213/150‑1 (MQ=255)

                                                                                        cgcgCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTAGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTccc                                                               <  2:3111133/150‑1 (MQ=255)

                                                                                         gcgcCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTcccc                                                              <  2:3945000/150‑1 (MQ=255)

                                                                                          cgcCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCg                                                             <  1:4029199/150‑1 (MQ=255)

                                                                                           gcCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGt                                                            >  1:4560015/1‑150 (MQ=255)

                                                                                            ccGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTg                                                           <  1:1387094/150‑1 (MQ=255)

                                                                                              gTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTgcc                                                         <  1:3745872/150‑1 (MQ=255)

                                                                                               ttCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTgccg                                                        <  1:2939999/150‑1 (MQ=255)

                                                                                                 ccACGGTCGCGACAGCCGCAATGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGTCGTCCCCGTgccgcc                                                      >  2:899572/1‑150 (MQ=255)

                                                                                                      gTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGtt                                                 <  2:2710589/150‑1 (MQ=255)

                                                                                                      gTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGtt                                                 <  1:1480687/150‑1 (MQ=255)

                                                                                                      gTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGtt                                                 >  2:4617073/1‑150 (MQ=255)

                                                                                                           gACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTg                                            >  2:1126750/1‑150 (MQ=255)

                                                                                                             cAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGAt                                          >  1:211013/1‑150 (MQ=255)

                                                                                                              aGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGAtt                                         >  1:2336627/1‑150 (MQ=255)

                                                                                                                 cGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGAc                                      >  2:189571/1‑150 (MQ=255)

                                                                                                                  gCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACg                                     <  1:3732878/150‑1 (MQ=255)

                                                                                                                   cTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGt                                    <  2:3854689/150‑1 (MQ=255)

                                                                                                                   cTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTAGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGt                                    >  2:3569352/1‑150 (MQ=255)

                                                                                                                     ttGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTa                                  >  1:3800272/1‑150 (MQ=255)

                                                                                                                      tGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTaa                                 >  2:1844575/1‑150 (MQ=255)

                                                                                                                       ggCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAAc                                <  1:596063/150‑1 (MQ=255)

                                                                                                                        gCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACg                               >  2:60125/1‑150 (MQ=255)

                                                                                                                          aCCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGc                             <  1:3564298/150‑1 (MQ=255)

                                                                                                                            cAATCCGTTCTGTGTGGTTTTCCCTAGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGt                           <  2:4145072/150‑1 (MQ=255)

                                                                                                                               tCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGcc                        >  2:4203519/1‑150 (MQ=255)

                                                                                                                                 cGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCCGGTTGCCTGATTGACGTTAACGGCGTGccta                      >  1:2721209/1‑148 (MQ=255)

                                                                                                                                 cGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGccta                      >  1:1160108/1‑148 (MQ=255)

                                                                                                                                       tagagtttttCCCTCGTAAAGATAATTTCCCGCAGTTGCTTGATTACGCCACAAGCGCCATTGCATTTGTCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGTTTGCCTGATTGACGTTAACGGCGTGCCTACGAACa                >  2:2309153/7‑150 (MQ=255)

                                                                                                                                         gtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAAt              >  2:51571/1‑150 (MQ=255)

                                                                                                                                         gtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAAt              >  1:935706/1‑150 (MQ=255)

                                                                                                                                            gTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcg           <  2:486282/150‑1 (MQ=255)

                                                                                                                                            gTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcg           >  2:3284743/1‑150 (MQ=255)

                                                                                                                                            gTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcg           >  2:1098258/1‑150 (MQ=255)

                                                                                                                                             ttttCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcgg          >  2:3467273/1‑150 (MQ=255)

                                                                                                                                               ttCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCcggcg        >  1:2599172/1‑150 (MQ=255)

                                                                                                                                                tCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcggcgg       <  2:4192955/150‑1 (MQ=255)

                                                                                                                                                  ccTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCAAAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTa     >  1:3475938/1‑150 (MQ=255)

                                                                                                                                                   cTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTaa    <  1:3675770/150‑1 (MQ=255)

                                                                                                                                                   cTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTaa    <  1:4338324/150‑1 (MQ=255)

                                                                                                                                                    tCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAAt   <  1:2884157/150‑1 (MQ=255)

TCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATG  >  CP009273/832168‑832466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`