Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,024,387 C→A 14.0% D347Y (GAT→TAT)  rlmI ← 23S rRNA m(5)C1962 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,024,3870CA14.0% 968.2 / 89.2 285D347Y (GAT→TAT) rlmI23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (150/95);  new base A (22/18);  total (172/113)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 285 total aligned reads displayed.

ACGCGACAGGCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCGGGTCCATCACG  >  CP009273/1024242‑1024535
                                                                                                                                                 |                                                                                                                                                    
aCGCGACAGGCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTg                                                                                                                                                  <  1:1854262/150‑1 (MQ=255)
 cgcgACAGGCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTgg                                                                                                                                                 >  1:540427/1‑150 (MQ=255)
   cgACAGGCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGtc                                                                                                                                               >  2:3183370/1‑150 (MQ=255)
       aGGCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGtcatca                                                                                                                                           >  1:2085221/1‑150 (MQ=255)
       aGGCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGtcatca                                                                                                                                           <  2:1223406/150‑1 (MQ=255)
       aGGCAAACCCTTTCAGATAGAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGtcatca                                                                                                                                           <  2:3546246/150‑1 (MQ=255)
        ggCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAg                                                                                                                                          >  1:4432195/1‑150 (MQ=255)
        ggCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAg                                                                                                                                          <  1:225397/150‑1 (MQ=255)
          cAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGAc                                                                                                                                        >  2:1701379/1‑150 (MQ=255)
           aaaCCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGAcc                                                                                                                                       <  1:611257/150‑1 (MQ=255)
             aCCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCgg                                                                                                                                     >  2:2937074/1‑150 (MQ=255)
              cccTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGa                                                                                                                                    <  1:4128710/150‑1 (MQ=255)
               acTTTCAGATATAGACCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTATATAAATTGTACATCACGACCGGCATCAATTTCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGaa                                                                                                                                   <  1:3163234/149‑1 (MQ=255)
                   tCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAgg                                                                                                                               >  2:2379901/1‑150 (MQ=255)
                     aGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGgag                                                                                                                             >  2:3277414/1‑150 (MQ=255)
                     aGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGgag                                                                                                                             >  1:1275038/1‑150 (MQ=255)
                      gATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGgaga                                                                                                                            >  2:665511/1‑150 (MQ=255)
                       atatAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGaa                                                                                                                           >  2:1568187/1‑150 (MQ=255)
                       atatAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGaa                                                                                                                           <  1:1501695/150‑1 (MQ=255)
                         ataGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGTGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAg                                                                                                                         >  2:2438980/1‑150 (MQ=255)
                          taGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGt                                                                                                                        >  2:3690069/1‑150 (MQ=255)
                          taGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGt                                                                                                                        >  2:4343170/1‑150 (MQ=255)
                          taGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGt                                                                                                                        >  1:2341475/1‑150 (MQ=255)
                            gCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCa                                                                                                                      <  1:266929/150‑1 (MQ=255)
                            gCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCa                                                                                                                      <  2:2331244/150‑1 (MQ=255)
                               cTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGga                                                                                                                   <  2:785912/150‑1 (MQ=255)
                                  ccGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGaa                                                                                                                <  1:2160626/150‑1 (MQ=255)
                                  ccGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGaa                                                                                                                >  2:1313919/1‑150 (MQ=255)
                                   cGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTTCTCTATAAATTGTACATCACGACAGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAAt                                                                                                               <  2:4073570/150‑1 (MQ=255)
                                     gATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATAc                                                                                                             <  2:2615298/150‑1 (MQ=255)
                                        aGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGc                                                                                                          >  2:1957827/1‑150 (MQ=255)
                                               gATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCttcatt                                                                                                   >  2:389909/1‑150 (MQ=255)
                                               gATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCttcatt                                                                                                   >  2:1559974/1‑150 (MQ=255)
                                                aTCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCttcattc                                                                                                  <  2:4176138/150‑1 (MQ=255)
                                                 tCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCttcattca                                                                                                 <  2:2975608/150‑1 (MQ=255)
                                                  cACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTcag                                                                                                <  2:4600397/150‑1 (MQ=255)
                                                    ccGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTcagca                                                                                              >  2:1140908/1‑150 (MQ=255)
                                                    ccGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTcagca                                                                                              >  1:2359695/1‑150 (MQ=255)
                                                      ggATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCagcagc                                                                                            >  1:305689/1‑150 (MQ=255)
                                                         tgatCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGa                                                                                         <  1:699978/150‑1 (MQ=255)
                                                           atCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAAt                                                                                       <  1:3623578/150‑1 (MQ=255)
                                                              ggCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGc                                                                                    >  2:2565564/1‑150 (MQ=255)
                                                              ggCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGc                                                                                    >  1:4437561/1‑150 (MQ=255)
                                                              ggCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGc                                                                                    >  1:3538723/1‑150 (MQ=255)
                                                               gCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGcc                                                                                   <  1:3093650/150‑1 (MQ=255)
                                                               gCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGcc                                                                                   <  1:1687582/150‑1 (MQ=255)
                                                                cTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCa                                                                                  >  2:4399274/1‑150 (MQ=255)
                                                                cTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCa                                                                                  >  2:1785683/1‑150 (MQ=255)
                                                                 tGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAg                                                                                 >  1:1956605/1‑150 (MQ=255)
                                                                 tGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAg                                                                                 >  1:997810/1‑150 (MQ=255)
                                                                 tGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAg                                                                                 >  2:2798031/1‑150 (MQ=255)
                                                                  gCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGc                                                                                >  2:4560626/1‑150 (MQ=255)
                                                                   ccTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCa                                                                               >  2:3144695/1‑150 (MQ=255)
                                                                    cTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCAt                                                                              >  2:377646/1‑150 (MQ=255)
                                                                    cTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCAt                                                                              >  2:2412934/1‑150 (MQ=255)
                                                                     tGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATg                                                                             >  2:4502453/1‑150 (MQ=255)
                                                                     tGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATg                                                                             >  2:539211/1‑150 (MQ=255)
                                                                     tGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATg                                                                             >  2:3623011/1‑150 (MQ=255)
                                                                     tGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATg                                                                             >  1:2570606/1‑150 (MQ=255)
                                                                       aCGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGtt                                                                           <  2:3472911/150‑1 (MQ=255)
                                                                         ggAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGa                                                                         >  2:2107575/1‑150 (MQ=255)
                                                                          gAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGAt                                                                        <  1:888546/150‑1 (MQ=255)
                                                                             cTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTc                                                                     <  2:1028500/150‑1 (MQ=255)
                                                                                ctctATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCttt                                                                  <  2:4606296/150‑1 (MQ=255)
                                                                                   tataAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTtata                                                               >  1:3919585/1‑150 (MQ=255)
                                                                                   tataAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTtata                                                               >  1:1053125/1‑150 (MQ=255)
                                                                                   tataAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTtata                                                               <  1:3384587/150‑1 (MQ=255)
                                                                                       aaTTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCa                                                           >  2:3997464/1‑150 (MQ=255)
                                                                                       aaTTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCa                                                           >  2:3321479/1‑150 (MQ=255)
                                                                                       aaTTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGAAGTCTTTATAACCa                                                           >  2:548512/1‑150 (MQ=255)
                                                                                         ttGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACg                                                         <  1:4307551/150‑1 (MQ=255)
                                                                                          tGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGa                                                        >  1:1071326/1‑150 (MQ=255)
                                                                                             aCATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGacac                                                     >  2:1086731/1‑150 (MQ=255)
                                                                                              cATCACGACCGGCATCATTTGCGGCATCCGCGATGATTTTCTGATATAAATCGCTGGTCATCAGACCGGATCAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGAAAcg                                                    <  2:1667767/150‑1 (MQ=255)
                                                                                               aTCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACAcgc                                                   >  1:400939/1‑150 (MQ=255)
                                                                                                 cACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACgcgc                                                 >  1:917309/1‑150 (MQ=255)
                                                                                                   cGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGACTACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGccc                                               >  2:2839903/1‑150 (MQ=255)
                                                                                                     aCCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCAt                                             <  1:2738060/150‑1 (MQ=255)
                                                                                                       cGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCa                                           <  2:3654782/150‑1 (MQ=255)
                                                                                                        ggCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCaa                                          >  2:2296735/1‑150 (MQ=255)
                                                                                                            tCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTgg                                      >  2:478681/1‑150 (MQ=255)
                                                                                                              aaTTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCt                                    <  2:3530112/150‑1 (MQ=255)
                                                                                                               aTTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCtt                                   >  1:814832/1‑150 (MQ=255)
                                                                                                                 tGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCtttt                                 <  2:2198702/150‑1 (MQ=255)
                                                                                                                  gCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTa                                >  1:3248137/1‑150 (MQ=255)
                                                                                                                    ggCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTAtt                              <  2:415821/150‑1 (MQ=255)
                                                                                                                      cATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATtct                            <  2:1724263/150‑1 (MQ=255)
                                                                                                                        tCCGCGATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTca                          >  1:3780473/1‑150 (MQ=255)
                                                                                                                          cgcgATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTcaac                        >  1:4610916/1‑150 (MQ=255)
                                                                                                                          cgcgATGATTTTCTGAAATAAATAGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTcaac                        >  1:4192180/1‑150 (MQ=255)
                                                                                                                          cgagatgatTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACTACACGCGCCCATCAACTGGCTTTTATTCTcaac                        >  2:3010100/4‑150 (MQ=255)
                                                                                                                                   tttCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTcgg               <  1:2445647/150‑1 (MQ=255)
                                                                                                                                     tCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTcggcg             >  1:4333358/1‑150 (MQ=255)
                                                                                                                                      cTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTcggcgg            <  1:2964956/150‑1 (MQ=255)
                                                                                                                                       tGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCggg           >  1:1182992/1‑150 (MQ=255)
                                                                                                                                        gAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCGGGt          <  1:2776396/150‑1 (MQ=255)
                                                                                                                                         aaataaatCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCGGGTc         <  2:952700/150‑1 (MQ=255)
                                                                                                                                          aataaatCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCGGGTcc        >  2:1056073/1‑150 (MQ=255)
                                                                                                                                              aatCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCGGGTCCATCa    >  2:2791828/1‑150 (MQ=255)
                                                                                                                                                tCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCGGGTCCATCACg  >  2:3882666/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACGCGACAGGCAAACCCTTTCAGATATAGCCCTTCCGGATAGGTAGCGATCACCGGATGATCGGCTGCCTGACGGAACTGCTCTATAAATTGTACATCACGACCGGCATCAATTGCGGCATCCGCGATGATTTTCTGAAATAAATCGCTGGTCATCAGACCGGAACAGGAGAAAGTCAGGAGAATACCGCCTTCATTCAGCAGCTGAATCGCCAGCATGTTGATGTCTTTATAACCACGACACGCGCCCATCAACTGGCTTTTATTCTCAACAAACTTCGGCGGGTCCATCACG  >  CP009273/1024242‑1024535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑