Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,068,091 G→T 19.6% Q77K (CAG→AAG)  rutB ← ureidoacrylate amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,068,0910GT19.6% 783.1 / 120.0 267Q77K (CAG→AAG) rutBureidoacrylate amidohydrolase
Reads supporting (aligned to +/- strand):  ref base G (115/99);  new base T (24/29);  total (139/128)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

AGGCTGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGT  >  CP009273/1067942‑1068240
                                                                                                                                                     |                                                                                                                                                     
aGGCTGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTg                                                                                                                                                       >  1:1054887/1‑150 (MQ=255)
aGGCTGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTg                                                                                                                                                       >  1:481029/1‑150 (MQ=255)
aGGCTGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTg                                                                                                                                                       >  1:958535/1‑150 (MQ=255)
  gCTGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGtt                                                                                                                                                     >  2:3945310/1‑150 (MQ=255)
  gCTGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGtt                                                                                                                                                     <  1:2411281/150‑1 (MQ=255)
    tGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCa                                                                                                                                                   <  1:2796591/150‑1 (MQ=255)
     gCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCAt                                                                                                                                                  >  2:4087257/1‑150 (MQ=255)
       ggCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATcc                                                                                                                                                >  1:386115/1‑150 (MQ=255)
          aCCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAg                                                                                                                                             <  2:1786278/150‑1 (MQ=255)
          aCCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAg                                                                                                                                             >  1:2654502/1‑150 (MQ=255)
                 cATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCAtttt                                                                                                                                      <  1:3657840/150‑1 (MQ=255)
                  aTCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTg                                                                                                                                     <  1:865454/150‑1 (MQ=255)
                  aTCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTg                                                                                                                                     >  1:2044076/1‑150 (MQ=255)
                  aTCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTg                                                                                                                                     >  1:714913/1‑150 (MQ=255)
                      accaGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAAc                                                                                                                                 >  1:3935793/1‑150 (MQ=255)
                      accaGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAAc                                                                                                                                 <  1:2585897/150‑1 (MQ=255)
                        caGTTTATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTTCTTTCGCATGGTTTTCAGGGCGTTCGATTTTTGAAAATTCGGTGTGCCTGGTCCGCCAGCATCGACATACTTTTCATCCCAGCCATTTTGAAACCa                                                                                                                               <  2:1190116/150‑1 (MQ=255)
                         aGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAg                                                                                                                              <  1:3755579/150‑1 (MQ=255)
                         aGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAg                                                                                                                              <  1:315796/150‑1 (MQ=255)
                          gTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAga                                                                                                                             <  1:3655653/150‑1 (MQ=255)
                             gATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAgatga                                                                                                                          >  1:2131096/1‑150 (MQ=255)
                               tAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATc                                                                                                                        >  1:4597409/1‑150 (MQ=255)
                                 aTCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAg                                                                                                                      <  2:258398/150‑1 (MQ=255)
                                  tCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGc                                                                                                                     >  2:4316206/1‑150 (MQ=255)
                                      aGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATcc                                                                                                                 >  1:2531383/1‑150 (MQ=255)
                                      aGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATcc                                                                                                                 <  2:3967472/150‑1 (MQ=255)
                                      aGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATcc                                                                                                                 >  1:418312/1‑150 (MQ=255)
                                      aGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATcc                                                                                                                 >  1:957634/1‑150 (MQ=255)
                                      aGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATcc                                                                                                                 >  2:891950/1‑150 (MQ=255)
                                       ggAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATccc                                                                                                                >  2:411882/1‑150 (MQ=255)
                                        gAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCt                                                                                                               <  2:64418/150‑1 (MQ=255)
                                        gAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCt                                                                                                               >  2:1069944/1‑150 (MQ=255)
                                         aGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTg                                                                                                              >  1:3783872/1‑150 (MQ=255)
                                            cTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCg                                                                                                           <  1:2232358/150‑1 (MQ=255)
                                            cTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCg                                                                                                           <  2:2056714/150‑1 (MQ=255)
                                               tCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTc                                                                                                        <  1:3248089/150‑1 (MQ=255)
                                               tCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTc                                                                                                        <  2:1000035/150‑1 (MQ=255)
                                                 gCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTcgc                                                                                                      <  1:3394176/150‑1 (MQ=255)
                                                 gCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTcgc                                                                                                      <  2:2777510/150‑1 (MQ=255)
                                                    aGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCt                                                                                                   <  1:2205109/150‑1 (MQ=255)
                                                     gCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTg                                                                                                  <  1:736177/150‑1 (MQ=255)
                                                     gCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTg                                                                                                  <  1:3480265/150‑1 (MQ=255)
                                                            ccccTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCAc                                                                                           >  2:360201/1‑150 (MQ=255)
                                                            ccccTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCAc                                                                                           <  2:2001281/150‑1 (MQ=255)
                                                             cccTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCAcg                                                                                          >  2:1538430/1‑150 (MQ=255)
                                                               cTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCAcggc                                                                                        <  2:3384264/150‑1 (MQ=255)
                                                               cTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCAcggc                                                                                        <  2:3512052/150‑1 (MQ=255)
                                                                tGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCAcggcg                                                                                       <  2:1519227/150‑1 (MQ=255)
                                                                 gCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCAcggcgg                                                                                      >  2:3334028/1‑150 (MQ=255)
                                                                   aGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGtt                                                                                    <  2:4531825/150‑1 (MQ=255)
                                                                     cTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTg                                                                                  >  1:1881411/1‑150 (MQ=255)
                                                                        cGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAt                                                                               >  2:3747243/1‑150 (MQ=255)
                                                                        cGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAt                                                                               >  1:1057917/1‑150 (MQ=255)
                                                                        cGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAAt                                                                               >  2:848917/1‑150 (MQ=255)
                                                                         ggCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATg                                                                              >  2:791210/1‑150 (MQ=255)
                                                                          gctgctTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGt                                                                             <  1:1734356/150‑1 (MQ=255)
                                                                               ttACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCa                                                                        <  1:1005832/150‑1 (MQ=255)
                                                                                   gCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGa                                                                    >  1:1423917/1‑150 (MQ=255)
                                                                                      tGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCg                                                                 >  2:603734/1‑150 (MQ=255)
                                                                                       ggTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCgg                                                                >  2:4399632/1‑150 (MQ=255)
                                                                                       ggTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCgg                                                                >  2:2699635/1‑150 (MQ=255)
                                                                                           ttCAGGGATTTCGATTTATGAAAATTCGGTGAGCCGGGTACTCCAGCCTCGACATTCTTTTCATCCGAGCCTTTTTGAATCCAGATGATCAGTATCCCTGCCGCTAGCGCTGCGGTCACGGCGGTTTGAATGTTGGGATTTAACGGGCGa                                                            <  2:2191232/150‑1 (MQ=255)
                                                                                            tCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAg                                                           <  1:4211932/150‑1 (MQ=255)
                                                                                              aGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTg                                                         >  1:2060203/1‑150 (MQ=255)
                                                                                                 gCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGtt                                                      <  1:2930458/150‑1 (MQ=255)
                                                                                                   gTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTga                                                    >  1:1410411/1‑150 (MQ=255)
                                                                                                     tCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTgaga                                                  <  2:1919159/150‑1 (MQ=255)
                                                                                                        aTTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACAt                                               >  1:388285/1‑150 (MQ=255)
                                                                                                          ttATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCa                                             >  1:4043079/1‑150 (MQ=255)
                                                                                                            aTGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCaaa                                           >  1:1809375/1‑150 (MQ=255)
                                                                                                            aTGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCaaa                                           <  1:517316/150‑1 (MQ=255)
                                                                                                              gAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAAcc                                         >  2:4255911/1‑150 (MQ=255)
                                                                                                               aaaaTTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAAccc                                        <  1:4248768/150‑1 (MQ=255)
                                                                                                               aaaaTTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAAccc                                        >  1:553601/1‑150 (MQ=255)
                                                                                                                   tgcGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCg                                    >  1:1666185/3‑150 (MQ=255)
                                                                                                                         gAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCt                              >  1:637348/1‑150 (MQ=255)
                                                                                                                          aGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTa                             <  2:2098296/150‑1 (MQ=255)
                                                                                                                             cGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGt                          >  1:270680/1‑150 (MQ=255)
                                                                                                                              gggTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTa                         <  2:385978/150‑1 (MQ=255)
                                                                                                                              gggTCCGCCAGCCTCGACATACTTTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTa                         >  1:3207158/1‑150 (MQ=255)
                                                                                                                              gggTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTa                         <  1:810333/150‑1 (MQ=255)
                                                                                                                                 tCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgcc                      <  2:292026/150‑1 (MQ=255)
                                                                                                                                  ccGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgccg                     >  2:3445560/1‑150 (MQ=255)
                                                                                                                                    gCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgccgcc                   >  1:708141/1‑150 (MQ=255)
                                                                                                                                    gCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgccgcc                   >  1:3028254/1‑150 (MQ=255)
                                                                                                                                    gCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgccgcc                   >  1:1146498/1‑150 (MQ=255)
                                                                                                                                    gCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgccgcc                   >  2:1753283/1‑150 (MQ=255)
                                                                                                                                     ccAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCt                  >  2:4471707/1‑150 (MQ=255)
                                                                                                                                     ccAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCt                  <  1:1611213/150‑1 (MQ=255)
                                                                                                                                      cAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTAGGCAATGACCGGGCGAGTGGTTGAGACATAAAACCCGGCGAGATCTAAGTAGCCGCCTg                 >  1:2610888/1‑150 (MQ=255)
                                                                                                                                       aGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgc                       <  2:2754385/143‑1 (MQ=255)
                                                                                                                                       aGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAgc                       >  1:2754702/1‑143 (MQ=255)
                                                                                                                                          cTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGTGCTAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGt             >  2:5409/1‑150 (MQ=255)
                                                                                                                                          cTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGt             >  1:4506957/1‑150 (MQ=255)
                                                                                                                                           tCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTg            >  2:2975588/1‑150 (MQ=255)
                                                                                                                                               cATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCAt        >  1:236181/1‑150 (MQ=255)
                                                                                                                                                  aCTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAg     <  1:4316206/150‑1 (MQ=255)
                                                                                                                                                    tGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCg   <  1:591647/150‑1 (MQ=255)
                                                                                                                                                    tGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCg   >  1:1850862/1‑150 (MQ=255)
                                                                                                                                                     gTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGt  >  2:1704701/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGCTGCGGCACCAGTTCATCCACCAGTTGATAATCCCAGGAGCCTTTCGCCAGCAATTTCCCCTGCAGCTGCGGCTGCTTACGCATGGTTTTCAGGGCGTTCGATTTATGAAAATTCGGTGAGCCGGGTCCGCCAGCCTCGACATACTGTTCATCCCAGCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGT  >  CP009273/1067942‑1068240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑