Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,120,612 A→T 16.2% F57L (TTT→TTA)  mdtH ← multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,120,6120AT16.2% 696.6 / 114.5 251F57L (TTT→TTA) mdtHmultidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand):  ref base A (112/98);  new base T (20/21);  total (132/119)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

AGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTT  >  CP009273/1120463‑1120758
                                                                                                                                                     |                                                                                                                                                  
aGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCAATGTGGCGAATCCGGCGGCGCGCAACAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATa                                                                                                                                                    >  1:979739/1‑150 (MQ=255)
   cATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAAt                                                                                                                                                 >  1:1015526/1‑150 (MQ=255)
   cATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAAt                                                                                                                                                 >  1:2814962/1‑150 (MQ=255)
    aTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAAtt                                                                                                                                                >  2:3862388/1‑150 (MQ=255)
    aTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAAtt                                                                                                                                                >  2:1536100/1‑150 (MQ=255)
       aaaaCCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGc                                                                                                                                             >  2:3040267/1‑150 (MQ=255)
            cacaATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGa                                                                                                                                        <  2:907134/150‑1 (MQ=255)
             acaATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGAc                                                                                                                                       >  2:1301918/1‑150 (MQ=255)
               aaTAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCg                                                                                                                                     <  2:75455/150‑1 (MQ=255)
               aaTAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCg                                                                                                                                     <  2:4331604/150‑1 (MQ=255)
                 tAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCgag                                                                                                                                   <  1:3109534/150‑1 (MQ=255)
                  aGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCgaga                                                                                                                                  >  2:3646762/1‑150 (MQ=255)
                         gTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATAc                                                                                                                           >  2:2898299/1‑150 (MQ=255)
                         gTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATAc                                                                                                                           >  1:2706912/1‑150 (MQ=255)
                          ttCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATAcc                                                                                                                          <  1:1131362/150‑1 (MQ=255)
                             gTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGAc                                                                                                                       >  1:3241680/1‑150 (MQ=255)
                               gggCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCa                                                                                                                     >  1:4415459/1‑150 (MQ=255)
                                ggCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCAt                                                                                                                    >  2:3775583/1‑150 (MQ=255)
                                ggCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCAt                                                                                                                    <  2:1070336/150‑1 (MQ=255)
                                   gATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGAc                                                                                                                 <  1:928889/150‑1 (MQ=255)
                                   gATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAc                                                                                                                 >  2:51006/1‑150 (MQ=255)
                                    aTACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGAcg                                                                                                                >  2:617945/1‑150 (MQ=255)
                                    aTACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAcg                                                                                                                <  2:4463003/150‑1 (MQ=255)
                                    aTACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAcg                                                                                                                >  1:4263902/1‑150 (MQ=255)
                                     tACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCCTACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAcgg                                                                                                               >  1:2914002/1‑150 (MQ=255)
                                      aCCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAcggc                                                                                                              <  1:104997/150‑1 (MQ=255)
                                        ccATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAcggcgg                                                                                                            <  2:2356485/150‑1 (MQ=255)
                                        ccATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAcggcgg                                                                                                            >  2:1481352/1‑150 (MQ=255)
                                        ccATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGAcggcgg                                                                                                            >  1:4145933/1‑150 (MQ=255)
                                          aTTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGgcc                                                                                                          <  2:128354/150‑1 (MQ=255)
                                           ttGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGgccc                                                                                                         <  1:2204396/150‑1 (MQ=255)
                                            tgtgGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGgccca                                                                                                        <  1:4275139/150‑1 (MQ=255)
                                            tgtgGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGgccca                                                                                                        <  1:2023233/150‑1 (MQ=255)
                                              tgGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCCTGACGGCGgcccagc                                                                                                      >  2:4241537/1‑150 (MQ=255)
                                                  gAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCAt                                                                                                  >  1:3894661/1‑150 (MQ=255)
                                                  gAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCAt                                                                                                  >  1:2755002/1‑150 (MQ=255)
                                                  gAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCAt                                                                                                  >  1:3605191/1‑150 (MQ=255)
                                                    aTCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCAttt                                                                                                >  1:587527/1‑150 (MQ=255)
                                                    aTCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCAttt                                                                                                <  1:4594478/150‑1 (MQ=255)
                                                     tCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTg                                                                                               <  2:3965250/150‑1 (MQ=255)
                                                      ccGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGa                                                                                              >  1:2606008/1‑150 (MQ=255)
                                                       cggcggCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGAt                                                                                             <  1:2182315/150‑1 (MQ=255)
                                                        ggcggcGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATc                                                                                            >  2:4643083/1‑150 (MQ=255)
                                                          cggcgCGCATCAGCATACCGGTAACACTCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCaa                                                                                          <  1:2779860/150‑1 (MQ=255)
                                                          cggcgCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCaa                                                                                          <  2:1961819/150‑1 (MQ=255)
                                                           ggcgCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAAc                                                                                         >  1:4606468/1‑150 (MQ=255)
                                                              gcgcATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGaa                                                                                      >  1:4632660/1‑150 (MQ=255)
                                                              gcgcATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGaa                                                                                      <  1:1625909/150‑1 (MQ=255)
                                                                  aTCAGCCTACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCgg                                                                                  >  2:323878/1‑150 (MQ=255)
                                                                  aTCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCgg                                                                                  <  2:680577/150‑1 (MQ=255)
                                                                   tCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGa                                                                                 >  2:2122093/1‑150 (MQ=255)
                                                                   tCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGa                                                                                 <  2:762494/150‑1 (MQ=255)
                                                                       cATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATaga                                                                             >  2:3360097/1‑150 (MQ=255)
                                                                       cATACCGGTAACAAACATCGGTTTGGCACCAAAGCGGTCGGCAAATGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCAATTTGATCAACGAAGCGGATaga                                                                             >  2:431209/1‑150 (MQ=255)
                                                                          aCCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGAt                                                                          <  2:1735111/150‑1 (MQ=255)
                                                                          aCCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGAt                                                                          >  1:1126991/1‑150 (MQ=255)
                                                                           ccGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATc                                                                         <  2:2622169/150‑1 (MQ=255)
                                                                            cGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCa                                                                        <  1:4130054/150‑1 (MQ=255)
                                                                             ggTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAg                                                                       <  2:4182387/150‑1 (MQ=255)
                                                                              gTAACTATCATCGGTTTGGCAACTTAGCGGTCGTCTATTGCACCGACGTATATACCCAGACCTTGCTGATTTTATTGGCGTAGTCCGAGTGCAATACCGACCATGACGGCGGCCCTGCCCTTTTGATCAACGAAGCGGATAGATATCAGc                                                                      <  2:4292425/150‑1 (MQ=255)
                                                                                aaCAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCgg                                                                    >  2:539507/1‑150 (MQ=255)
                                                                                 aCAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCggg                                                                   <  2:3551962/150‑1 (MQ=255)
                                                                                   aaTCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGaa                                                                 >  1:3402071/1‑150 (MQ=255)
                                                                                       atcGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGaca                                                             <  1:738765/150‑1 (MQ=255)
                                                                                       atcGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGaca                                                             >  1:494260/1‑150 (MQ=255)
                                                                                             ttGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAg                                                       <  2:2587841/150‑1 (MQ=255)
                                                                                                 cACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAAcc                                                   >  2:2972374/1‑150 (MQ=255)
                                                                                                     aaaGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAg                                               >  1:997876/1‑150 (MQ=255)
                                                                                                     aaaGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAg                                               >  1:3223538/1‑150 (MQ=255)
                                                                                                     aaaGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAg                                               >  1:1285544/1‑150 (MQ=255)
                                                                                                     aaaGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAg                                               >  1:3550050/1‑150 (MQ=255)
                                                                                                       aGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCa                                             >  1:3725266/1‑150 (MQ=255)
                                                                                                        gCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCAc                                            <  1:4587109/150‑1 (MQ=255)
                                                                                                         cGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACg                                           <  1:3646762/150‑1 (MQ=255)
                                                                                                         cGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACg                                           >  2:3471543/1‑150 (MQ=255)
                                                                                                         cGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACg                                           <  1:2402237/150‑1 (MQ=255)
                                                                                                         cGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACg                                           >  2:1148006/1‑150 (MQ=255)
                                                                                                                cAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCa                                    >  1:2993009/1‑150 (MQ=255)
                                                                                                                 aaTTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCat                                   >  2:699569/1‑150 (MQ=255)
                                                                                                                 aaTTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCat                                   >  1:2454451/1‑150 (MQ=255)
                                                                                                                       aCCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATc                             <  2:2942955/150‑1 (MQ=255)
                                                                                                                       aCCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATc                             <  2:3983479/150‑1 (MQ=255)
                                                                                                                         cgccgAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGa                           >  2:1702387/1‑150 (MQ=255)
                                                                                                                          gccgAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGAt                          <  1:3042513/150‑1 (MQ=255)
                                                                                                                             gAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAg                       >  2:551650/1‑150 (MQ=255)
                                                                                                                             gAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAg                       <  1:1253714/150‑1 (MQ=255)
                                                                                                                               aaaTACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCa                     >  1:28625/1‑150 (MQ=255)
                                                                                                                                aaTACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAg                    <  2:2318993/150‑1 (MQ=255)
                                                                                                                                aaTACCCAGACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAg                    <  2:952455/150‑1 (MQ=255)
                                                                                                                                aaTACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAg                    >  1:1786132/1‑150 (MQ=255)
                                                                                                                                    cccAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGaaa                >  1:2515276/1‑150 (MQ=255)
                                                                                                                                      cAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAata              >  1:3840793/1‑150 (MQ=255)
                                                                                                                                       aGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAatat             >  2:3576005/1‑150 (MQ=255)
                                                                                                                                       aGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAatat             >  2:239174/1‑150 (MQ=255)
                                                                                                                                        gACCTTGCTGAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATAtt            <  1:521928/150‑1 (MQ=255)
                                                                                                                                        gACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATAtt            >  1:2104086/1‑150 (MQ=255)
                                                                                                                                          ccTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTa          <  2:1759222/150‑1 (MQ=255)
                                                                                                                                            ttGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTAcc        <  2:3611142/150‑1 (MQ=255)
                                                                                                                                             tGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTAccc       <  1:4430881/150‑1 (MQ=255)
                                                                                                                                                 gAATTAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGt   >  1:329681/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTT  >  CP009273/1120463‑1120758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑